LOCUS       Exported                3927 bp ds-DNA     circular SYN 15-JAN-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   MRC LMB
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /note="/locus_tag=source:recombinant plasmid"
     CDS             403..1062
                     /locus_tag="AmpR"
                     /label=AmpR
                     /note="ApEinfo_fwdcolor=yellow ApEinfo_revcolor=yellow"
     rep_origin      1160..1842
                     /locus_tag="ColE1 origin"
                     /label=ApEinfo_fwdcolor=gray50 ApEinfo_revcolor=gray50
                     /note="ApEinfo_fwdcolor=gray50 ApEinfo_revcolor=gray50"
     primer_bind     2310..2329
                     /locus_tag="T7"
                     /label=ApEinfo_fwdcolor=cyan ApEinfo_revcolor=green
                     /note="ApEinfo_fwdcolor=cyan ApEinfo_revcolor=green"
     misc_feature    2418..2423
                     /locus_tag="RBS (ribosome binding site)"
                     /label=ApEinfo_fwdcolor=#fffb05 ApEinfo_revcolor=#fffb05
                     /note="ApEinfo_fwdcolor=#fffb05 ApEinfo_revcolor=#fffb05"
     CDS             2435..3132
                     /codon_start=1
                     /locus_tag="GST tag"
                     /label=GST tag
                     /note="ApEinfo_fwdcolor=cyan ApEinfo_revcolor=cyan"
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDSS
                     GSENLYFQGS"
     primer_bind     2462..2479
                     /locus_tag="JBPR139"
                     /label=JBPR139
     primer_bind     2792..2809
                     /locus_tag="JBPR140"
                     /label=JBPR140
     primer_bind     3103..3120
                     /locus_tag="JBPR149 seq"
                     /label=JBPR149 seq
     misc_feature    3107..3127
                     /locus_tag="TEV cleavage site (ENLYFQ//G)"
                     /label=ApEinfo_fwdcolor=#ff6b01 ApEinfo_revcolor=#ff6b01
                     /note="ApEinfo_fwdcolor=#ff6b01 ApEinfo_revcolor=#ff6b01"
     primer_bind     3131..3151
                     /locus_tag="JBPR146"
                     /label=JBPR146
     CDS             3134..3748
                     /codon_start=1
                     /locus_tag="Rab1QL"
                     /label=Rab1QL
                     /translation="MSSMNPEYDYLFKLLLIGDSGVGKSSLLLRFADDTYTESYISTIG
                     VDFKIRTIELDGKTIKLQIWDTAGLERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQ
                     WLQEIDRYASENVNKLLVGNKSDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQ
                     SFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGC"
     primer_bind     3134..3151
                     /locus_tag="JBPR74"
                     /label=JBPR74
     primer_bind     complement(3190..3230)
                     /locus_tag="JBPR79"
                     /label=JBPR79
     primer_bind     3190..3230
                     /locus_tag="JBPR78"
                     /label=JBPR78
     primer_bind     3446..3463
                     /locus_tag="JBPR150"
                     /label=JBPR150
     primer_bind     complement(3490..3530)
                     /locus_tag="JBPR81"
                     /label=JBPR81
     primer_bind     3490..3530
                     /locus_tag="JBPR80"
                     /label=JBPR80
     primer_bind     complement(3725..3754)
                     /locus_tag="JBPR148"
                     /label=JBPR148
     primer_bind     complement(3725..3746)
                     /locus_tag="JBPR147"
                     /label=JBPR147
     primer_bind     complement(3725..3743)
                     /locus_tag="JBPR75"
                     /label=JBPR75
     misc_feature    3825..3847
                     /locus_tag="T7R (T7 terminator primer)"
                     /label=ApEinfo_fwdcolor=#fff80a ApEinfo_revcolor=#fff80a
                     /note="ApEinfo_fwdcolor=#fff80a ApEinfo_revcolor=#fff80a"
ORIGIN
        1 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
       61 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
      121 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
      181 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      241 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      301 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      361 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      421 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
      481 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
      541 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
      601 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
      661 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
      721 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
      781 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
      841 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
      901 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
      961 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     1021 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1081 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1141 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1201 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1261 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1321 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1381 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1441 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1501 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1561 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1621 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1681 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1741 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1801 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1861 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1921 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1981 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     2041 tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     2101 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     2161 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     2221 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     2281 cgcgaggcag gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg
     2341 gtttccctct agtgccggct ccggagagct ccaattggaa ttcgctagct ctagaaataa
     2401 ttttgtttaa ctttaagaag gagatataca taatatgtcc cctatactag gttattggaa
     2461 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga
     2521 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg
     2581 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat
     2641 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg
     2701 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat
     2761 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat
     2821 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac
     2881 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg
     2941 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat
     3001 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac
     3061 gtttggtggt ggcgaccatc ctccaaaatc ggattcttct ggttccgaaa acctgtattt
     3121 ccagggctcc catatgtcca gcatgaatcc cgaatatgat tatttattca agttacttct
     3181 gattggcgac tcaggggttg gaaagtcttc ccttcttctt aggtttgcag atgatacata
     3241 tacagaaagc tacatcagca caattggtgt ggatttcaaa ataagaacta tagagttaga
     3301 cgggaaaaca atcaagcttc aaatatggga cacagcaggc ctggaaagat ttcgaacaat
     3361 cacctccagt tattacagag gagcccatgg catcatagtt gtgtatgatg tgacagatca
     3421 ggagtccttc aataatgtta aacagtggct gcaggaaata gatcgttatg ccagtgaaaa
     3481 tgtcaacaaa ttgttggtag ggaacaaatc tgatctgacc acaaagaaag tagtagacta
     3541 cacaacagcg aaggaatttg ctgattccct tggaattccg tttttggaaa ccagtgctaa
     3601 gaatgcaacg aatgtagaac agtctttcat gacgatggca gctgagatta aaaagcgaat
     3661 gggtcccgga gcaacagctg gtggtgctga gaagtccaat gttaaaattc agagcactcc
     3721 agtcaagcag tcaggtggag gttgctaagg atccagatct aagcttggta ccacgcgtgc
     3781 gcgctgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     3841 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     3901 aaggaggaac tatagccgga taattct
//
