LOCUS       Exported                5293 bp ds-DNA     circular SYN 19-OCT-2018
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..3768
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
     source          4429..5293
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /label=source:synthetic DNA construct(1)
     rep_origin      595..1050
                     /label=M13 origin
     rep_origin      612..918
                     /label=F1 ori
     misc_feature    612..629
                     /label=ov242r
     rep_origin      1161..1749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    1161..1749
                     /label=pMB1 ori (from pBR322, without ROP gene)
     misc_feature    complement(1200..1307)
                     /label=RNAI
     misc_feature    1935..2075
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2520..3398)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPPRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     3200..3218
                     /label=SP19
     promoter        3425..3709
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     RBS             3736..3758
                     /label=araBAD promoter
                     /label=araBAD promoter(1)
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    3741..3749
                     /label=TRANSLATIONAL ENHANCER
     CDS             3766..3768
                     /codon_start=1
                     /product="Q67A= reduced Hydrolysis activity"
                     /label=Rab1b_3-174_Q67A
                     /translation="M"
     misc_feature    4015..4017
                     /label=New Feature
     primer_bind     4429..4437
                     /label=SLIM_Fb_Tail_HisTag
     primer_bind     4429..4437
                     /label=SLIM_Fa_Short_HisTag
     primer_bind     complement(4436..4453)
                     /label=R_pBAD_Duet_Backbone
     misc_feature    complement(4500..4517)
                     /label=pBAD reverse Primer
     terminator      4670..4756
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4848..4875
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     primer_bind     4875..4898
                     /label=F_pBAD_Duet_backbone
     promoter        4894..4985
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4986..5293,1..553)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGEPLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
       61 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
      121 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
      181 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
      241 aatggcaaca acgttgcgca aactattaac tggcgaacca cttactctag cttcccggca
      301 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
      361 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
      421 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
      481 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
      541 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttacgcgc
      601 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      661 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      721 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
      781 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
      841 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
      901 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga
      961 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     1021 attttaacaa aatattaacg tttacaattt aaaaggatct aggtgaagat cctttttgat
     1081 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1141 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1201 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1261 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     1321 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1381 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1441 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1501 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1561 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1621 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1681 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1741 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1801 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     1861 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     1921 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     1981 cgcatctggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     2041 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     2101 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     2161 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagaagg
     2221 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     2281 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
     2341 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
     2401 tctgctcatg tttgacagct tatcatcgat gcataatgtg cctgtcaaat ggacgaagca
     2461 gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat
     2521 tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc
     2581 gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca
     2641 acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg
     2701 ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga
     2761 tgtgacagac gcggcggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg
     2821 ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt
     2881 ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt
     2941 atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac
     3001 aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat
     3061 attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg
     3121 tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac
     3181 agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga
     3241 ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa
     3301 tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag
     3361 tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc
     3421 agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg
     3481 ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg
     3541 accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac
     3601 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta
     3661 gcggatacta cctgacgctt tttatcgcaa ctctctactg tttctccata cccgtttttt
     3721 tgggctagaa ataattttgt ttaactttaa gaaggagata tacatatggc tagtcgaggc
     3781 gcaacaagac ccaacgggcc aaatactgga aataaaatat ctcagttcaa actagtactt
     3841 ctgggagagt ccgctgttgg caaatcaagc ctagtgcttc gttttgtgaa aggccaattt
     3901 catgaatttc aagagagtac cattggggct gcttttctaa cccaaactgt atctcttgat
     3961 gacactacag taaagtttga aatatgggat acagctggtc tcgaacgata ccattagcta
     4021 gcaccaatgt actacagagg agcacaagca gccatagttg tatatgatat cacaaatgag
     4081 gagtcctttg caagagcaaa aaattgggtt aaagaacttc agaggcaagc aagtcctaac
     4141 attgtaatag ctttatcggg aaacaaggcc gacctagcaa ataaaagagc agtagatttc
     4201 caggaagcac agtcctatgc agatgacaat agtttattat tcatggagac atccgctaaa
     4261 acatcaatga atgtaaatga aatattcatg gcaatagcta aaaaattgcc aaagaatgaa
     4321 ccacaaaatc caggagcaaa ttctgccaga ggaagaggag tagaccttac cgaacccaca
     4381 caaccaacca ggaatcagtg tcatcaccac catcatcact aactcgaggg atccgggccc
     4441 tctagatgcg gccgcatgca taagcttggc tgttttggcg gatgagagaa gattttcagc
     4501 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc
     4561 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc
     4621 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
     4681 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     4741 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg
     4801 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct
     4861 gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa atacattcaa
     4921 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4981 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5041 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5101 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5161 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5221 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5281 acttggttga gta
//
