LOCUS       Exported               19999 bp ds-DNA     circular SYN 31-JUL-2012
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19999
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      complement(1483..1789)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(1811..1879)
                     /label=LacZ alpha
     primer_bind     1950..1967
                     /label=M13-fwd
     primer_bind     1976..1996
                     /label=T7
     misc_feature    complement(2092..2285)
                     /label=ADH1 teminator
     misc_feature    complement(2289..2690)
                     /label=zz
     misc_feature    complement(2691..2708)
                     /label=GAGA linker
     misc_feature    complement(2709..2852)
                     /label=linker
     misc_feature    complement(2769..2789)
                     /label=TEV
                     /label=TEV(2)
     misc_feature    complement(2811..2831)
                     /label=TEV
     misc_feature    2859..4668
                     /label=ScVPS15 coding minus stop
     misc_feature    4669..4671
                     /label=I851R
     misc_feature    4672..6820
                     /label=ScVPS15 coding minus stop
                     /label=ScVPS15 coding minus stop(1)
     misc_feature    6821
                     /label=T134A
     misc_feature    6822..7220
                     /label=ScVPS15 coding minus stop
                     /label=ScVPS15 coding minus stop(2)
     misc_feature    complement(7227..7687)
                     /label=GALGAPDH
     misc_feature    complement(7688..7693)
                     /label=ATG14L N256S
     misc_feature    complement(7996..10614)
                     /label=ScVps34_3-887
     misc_feature    complement(8144..8169)
                     /label=univeresal seq op0663f
     misc_feature    complement(8200..8220)
                     /label=sam0163_seq
     misc_feature    complement(8857..8877)
                     /label=sam0162
     misc_feature    9279..9296
                     /label=YO794(Munro lab)
     misc_feature    complement(9644..9662)
                     /label=Sam0161_seqf
     misc_feature    10093..10110
                     /label=YO793 (Munro lab)
     misc_feature    complement(10158..10182)
                     /label=op0662_seqr
     misc_feature    complement(10258..10287)
                     /label=sam010
     misc_feature    complement(10618..10620)
                     /label=ATG start
     misc_feature    complement(11408..11410)
                     /label=stop
                     /label=stop(1)
     misc_feature    complement(11417..13087)
                     /label=ScVPS30 coding
     misc_feature    complement(11990)
                     /label=mutation
                     /label=mutation(4)
     misc_feature    complement(12005)
                     /label=mutation
                     /label=mutation(8)
     misc_feature    complement(12035)
                     /label=mutation
                     /label=mutation(5)
     misc_feature    complement(12323)
                     /label=mutation
                     /label=mutation(7)
     misc_feature    complement(12372)
                     /label=mutation
                     /label=mutation(6)
     misc_feature    complement(12380)
                     /label=mutation
                     /label=mutation(3)
     misc_feature    complement(12904)
                     /label=mutation
                     /label=mutation(9)
     misc_feature    complement(13061)
                     /label=mutation
                     /label=mutation(2)
     misc_feature    complement(13064)
                     /label=mutation
                     /label=mutation(1)
     misc_feature    complement(13066)
                     /label=mutation
     misc_feature    complement(13148..13168)
                     /label=TEV
                     /label=TEV(1)
     misc_feature    complement(13190..13210)
                     /label=TEV
                     /label=TEV(3)
     misc_feature    complement(13241..13261)
                     /label=TEV cleavage site
     misc_feature    complement(13262..13660)
                     /label=proteinA tag x2
     misc_feature    complement(13661..13675)
                     /label=GAGA linker
                     /label=GAGA linker(1)
     misc_feature    complement(13679..13681)
                     /label=start
     misc_feature    complement(14472..14474)
                     /label=stop
     misc_feature    complement(14475..15797)
                     /label=ScVPS38 untagged
     misc_feature    complement(16280..16305)
                     /label=Kiyoshi's MCS
     primer_bind     complement(16395..16414)
                     /label=T3
     primer_bind     complement(16432..16452)
                     /label=M13-rev
     misc_binding    complement(16458..16480)
                     /label=LacO
     rep_origin      complement(16820..17502)
                     /direction=LEFT
                     /label=ColE1 origin
     misc_feature    16882..16900
                     /label=YO032
     CDS             complement(17600..18259)
                     /label=AmpR
     misc_recomb     complement(19552..19585)
                     /label=FRT
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtaccg ggccccccct cgagtacttc tcgacaagct
     2041 atcaccactt tgtacaagaa agctgggtct agatatctcg agtgcggccg ctgccggtag
     2101 aggtgtggtc aataagagcg acctcatgct atacctgaga aagcaacctg acctacagga
     2161 aagagttact caagaataag aattttcgtt ttaaaaccta agagtcactt taaaatttgt
     2221 atacacttat tttttttata acttatttaa taataaaaat cataaatcat aagaaattcg
     2281 cgagctcacg aattcgcgtc tactttcggc gcctgagcat catttagctt tttagcttct
     2341 gctaaaaggt tagcgctttg gcttgggtca tcttttaaac tttggatgaa ggcgtttcgt
     2401 tgttcttcgt ttaagttagg taaatgtaag atctcataga acgcgttttg ttgttctttg
     2461 ttgaatttgt tgtctacttt cggcgcctga gcatcattta gctttttagc ttctgctaaa
     2521 aggttagcgc tttggcttgg gtcatctttt aaactttgga tgaaggcgtt tcgttgttct
     2581 tcgtttaagt taggtaaatg taagatctca tagaacgcgt tttgttgttc tttgttgaat
     2641 ttgttgtcta cggcttcatc gtgggcgccc tggaagtaca ggttctcgct agcacccgcc
     2701 cctgctccgg atctagcttc gtcgtgttga gccaaagcag cagtcttcaa ttcaccctcg
     2761 agtaactcac cttggaagta caagttttca gaagcagtgg tcttcaactc gccttgaaag
     2821 tacaaatttt cagaggcagt agttggtcta gaggatcctt ggaagattcc aataagccct
     2881 gagttatcac aagcaaccag caaaggtgtc tcatcaactt cacatgtaga tatagaattt
     2941 ataatatcat gatgatatag agaatcatct actgaatgag tgctagtacg tttcattttt
     3001 ctcaataaca ttgtgagatt tgccgtaact tgtgtaggaa taaaaacgtc actgaatctt
     3061 gaaggactta ttactgcttt agaagaagaa agctcattta ggctaaacat aacaatggaa
     3121 cttgttgctt catcggtaag aagaatttta tcattagata ctgagatagt gcttagtgcg
     3181 tttaaagacc tgattccaca aaaatttaat tcttctaagc ctttctcaat tggtaaaaag
     3241 tgctccatag atggctgttc atcagaattt atgaaagcat actgacaatg ccccttaaca
     3301 aaattccata ttgttagaaa tgtttttgaa ctacctccta caacaattac agaattcttt
     3361 ccataaaact gacaaacctc cacatgcgtg attggtgcgt ggtccccaaa ggaccaactc
     3421 cttatcagca cgttgaaacg gatatcccat atatcaataa tacctctagt cgtccccaaa
     3481 attaggacac aacactcttc atcgatacag atgcttgaaa cggcaccatg ccttggagaa
     3541 ttctctataa tttgtaacct ctccagggtt ctaatatcaa atataataac ccttgacaaa
     3601 ttcgtcaatg ctactagtag agatttttcc tcattcacaa atgctctcat tctcactgcg
     3661 tattcatttt taccaaaatt cttcaagtta attttcctga tgcattcgca attcaaaaat
     3721 ttgacttcac tttcttgttg gtaatgatta acctttaata caattatttg tccatcttta
     3781 ctggaaacgg caaacgcgtc aaagttagga atcatggtta tctgagttac ggtagaggag
     3841 cagtcataag ttaaagaaga agagtaaacc tcgcccacga taatctcttt caggttccaa
     3901 atcttgatta caccttgatc tgaacctgtt attaaatagg gtgtttctcc tggagaaaca
     3961 gcagaagacg taatagaatt gggttcgttt tccatcaaag tagctatcaa cttgcccctc
     4021 aaattaccca tgtttggact ccgtacaatc tcttgaatag gcccaaactc cttataatct
     4081 ctcagaggag gtaaaatttt gaaagtcgat aggaattttt ctatgctttc aacgtcgcct
     4141 tcatagctgt tgctgacgac taccttcctt tctggtttaa catttgcatt atctttcaag
     4201 ccatgatttg gagaatgcat gtgataggac gttggttcta actgaacaaa aacgttcttc
     4261 aaattagaag tagtagtggc aatggactta tttttgaata ttagtgatcc atttatgtcc
     4321 atgacggtag atagctgttt gctaccgtta atatttagct tattgctatc tttctcactt
     4381 tcatcatatt tatatatctg ttggttctct aagttagaat cttgtccctc acttgtcgac
     4441 tcttcagcaa actctacatc aaagaaaatg tttcttggca taatattcgt aaggtttagg
     4501 ttaggtggtg agttctgtac taatggacgg taattactat tattatatgg aaaatcaggg
     4561 ttcgctgcca taactctcgc tgtccttatt acataacccc tcaaagccac aattttccaa
     4621 atatcttttt ctgttagccc aataatgtgg aacttatcaa tccaatttct gtcttcagca
     4681 gttaagggaa tttccttgtt gtcatgtgaa tagacggtgg atgatgtttt aatacccttc
     4741 agcgaggaac ttgatttcgt atctctttta ttaaatccat aatttttact tgagtaattt
     4801 tttgttataa attcgattct attatttccg aatgaatcta catgatttgt gatgattttt
     4861 ttccaaaata aagattttga cgctctaaca gaccaactac acaatagatt gtaaaccgat
     4921 cttgacactg gttgcttgca acagcttatc attgatttga agttgaactc aacgtcaaat
     4981 tcgaagaaag gccttattat tggatagaga atgcagtaca cttcggcttt tgataactta
     5041 ttaataattt ctataattat catcaaagta aactgtctta tccaattatt aggatgcaac
     5101 aacaagggag atgttgtttt tgatatatca atataatatt ttttcctaat caacccagtc
     5161 ttgaacaaag attttaagct ttgtaaaaca ctgatcacta ctaattcttc cgaatcagtg
     5221 atggtctgga ttaacaatgg taaaatatac tgttctaatg taacggtacc caaaagaatt
     5281 gatattccgg atattgtttg aattaaggaa actcgcaatg ctgggtcctt atcattaagg
     5341 taggttatta aatgactcag tataatatcg tttgttcttt ccctaccaaa aaatttacaa
     5401 agtggaagaa tgttttgcaa aagtgccatc tttacataag tatcattatc tgttaaaaaa
     5461 gagacagtta aatcttcgac actttggatc aattttgctg aatacttggt actgctttcc
     5521 atgatttccg tgttgttatc cattgagtta tcattgcagt gctgagcaaa tgtaaattct
     5581 tgaaatctat taatgataat ggccaagtcg ctcaaacaat tagcaaacac aatccttaaa
     5641 taattggtat tctgcctatt ggaaataagt aatcttttca gtctcggaag taagtagtcc
     5701 acaaatatat tctcgttcaa ttgattcaat tttcttacag aggtgagtac ctggattaat
     5761 gttaacaaag atagggcctg gacgtcctgg tcactatctt caaaacaaca tacgaaataa
     5821 ggtacgactc gatcaatttt attttcatca gaaacgaatt gtgcaaatac tgctaatagt
     5881 tctaaatttt taagtttcgt agcagtcgaa acaatacttc ttatactatg agacaaataa
     5941 gagataaaga gtaaggcaca ttcctcttta actgatttta tcttttcgga tactttggta
     6001 aactgctgta agactaaatg gtaattttgg gggaaatata agtttgtgtt taaaaaccga
     6061 ctaatttcta tctctatttt gtaagattcc aaaattgggg ggtctgaacc aatctcaccc
     6121 ccgtctttta ttaaaggaaa gtccaaacaa tgacatattt gggaaaaatc tctgtatatt
     6181 ttttccgtag tttcatctaa aagttttaca ttgtcttcca aggtactatt ggtgcaagtg
     6241 ttatctgata tcggtgtgct tgttgtcata gtaaccaaat ttctgaaata atcataaatg
     6301 aaagtgtaga aataatcggg gaagaaaatg ccacgatatt tattcagtag ttcatcacat
     6361 gaaagtcttt tggatggatc tagttgaatc atgtctagaa ccaagttcct taaatcggta
     6421 gaattcattt cctccatgag aaattccctg tttacgtcat atgaattact tttatatttg
     6481 aatagctgtg ataagttgaa gatgggtctt ccttcagcaa atatttctgc aataacacat
     6541 ccaagactaa atatgtccat ttctttagtt agcctaccat tgttagattt tccatcttgg
     6601 taaagtttag agttaaacct ctccggggct agataacagg ttcttctctt cgaggtgtcg
     6661 aagtagaata aaaattcacc tggattatct tcaggcaaat atacgggttt gataaatgca
     6721 gcaaaatctg tcaatataca ccaattccaa cttgttacta ggatattttc tgtctttata
     6781 tcaccatgga caatattcag attatgaatg tcctttaatg catttaacaa ctggaaagca
     6841 atgaatttca gttcaatgtc ttgtaagtaa ggtctcaaac tcaatctgtc atataaatta
     6901 ttttttaagt gctgccgtat catgtagccg gctctatttg tctcgatcaa tttactgtag
     6961 tttaaaacgt gcggtagttg tcccaactta aacgattgag cccttatacg ttggagaaaa
     7021 ggtcgtaagc tatattggtc ttttggttta ataaacactt tgataacaat ttcgccgtta
     7081 gggtccagtg ccttgcatgt ttttaagaat cttgatgagt ttaactgtga aacgtagtgt
     7141 acttcctcta agacatcgat atatgaaaaa atggctatgg aaggtgatgc ttggaccact
     7201 agtgataatt gtgcccccat ctcgagggtt tatttatgtg tgtttattcg aaactaagtt
     7261 cttggtgttt taaaactaaa aaaaagacta actataaaag tagaattcaa gaagtttaag
     7321 aaatagattt acagaattac aatcaatacc taccgtcttt atatacttat tagtcaagta
     7381 ggggaataat ttcagggaac tggtttaaac cttttttttc agtcgagata tctggttgtt
     7441 aatttgattc gttcatttga ggtttgtggg gccaggttac tgccaatttt cctcttcata
     7501 accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat
     7561 ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac ggaggagagt cttccttcgg
     7621 agggctgtca cccgctcggc ggcttctaat ccgtacgcat gctttttttg gatctttgca
     7681 aagatccccc gggcgccaga tcgatcttcg aggggaattc tcttaggatt cgattcacat
     7741 tcatcttttt ttagctacta ataggataaa ttataggaat ttataacaaa ttgaaatatg
     7801 gcaggcagca aaattaaaat tgtcttagtt ttttagtgta tagaagtgaa tagctatata
     7861 aagtatgtgt aaagttggta acgaaacgaa aaatagaaaa ggatattaca tgggaaaaca
     7921 ggttgtttac ggagaaatga aaagtatatt gtattttgta cgagctaaaa gtacagtggg
     7981 aacaaagtcg acttaggtcc gccagtattg tgccagatta tgtaaatgat caatcacgat
     8041 aggcagcaaa gcatttacac tatcattgat tagattctga aaatgcactg tggcatcttc
     8101 ttcggacata ttcaaattaa atctctctct tacacgtaat atagcaccat tgggatctat
     8161 tctgatatca ggtatgttcg acgtcttcat taattcaaac aggtttaaga ttaagcctgc
     8221 attccttctt aaaatcgaat atgccacaaa acagtagctg cgaaatttat cataatttga
     8281 tgattctgca cccccaaacg cctctataat ttgagggggc aatttcatta atggcggaaa
     8341 aggtttgggg tcctgaccca agatataacc aaagtctgcg tgaaaaaagt gcccatctgg
     8401 tgtgactagt aagttgtcta agtgcctatc gccgacacct aagatgtatg taataacaca
     8461 atagccagca caagatttga caaaattatc taaaacccag ccctgtacgc ctaatgtcgc
     8521 gttctcatca ggatagtgga gtttaaggta gccaagaata ccgtgatatt tgcttagtat
     8581 gctagctaat gtgtcattag ggataaattc aatggcacct tcttgtggtc ctgttgccaa
     8641 aattttgtat ggtgtcaatt tcaagtccac gttttcgttt tttaataact cattcattaa
     8701 acttatgatc tgtactacca gttgatcttg tctcaaatca tcgccaacct taaacattaa
     8761 gtgatatggt tggtttaagg tcgttttgaa tgttattttt agtggcgata aggagctttt
     8821 aaataccttc gaagtttcag gacaaacgtc gcatatcaac acatcagggt ctagtggtaa
     8881 agcaattggt cgtactttga caagaggcct gactttcgtc tccaataaat gcactagtaa
     8941 ttccatcttt ttggctgtag tatctttcag cctcttaatt gtttcacaac attccctgag
     9001 cacatttatt agcctgactt gatcgttcag tatattccga gattttttat ccagtctact
     9061 ccaaaaggaa cttaaaatct ggtctaaata tggcttgtcc tcagactcgg actttaaata
     9121 ccaatagaag aaacttccta accttgggtt gaccagtgct ctcctaatca aaaactcagc
     9181 caagggagaa atgacgattg gtagcgattc tgtcccggaa gtttccgatt cactcgagat
     9241 ggactttaat aatttttggt tggcatgcga tgtagatagt aacatagaat cacccgaaag
     9301 cttttgcgaa gatacggcat ccacaatagt gaattcactg ttagatttgt cggaaaaagt
     9361 ggacaggttc tcaaaacaga cagcttctac caactgtaat aagtacaatt ccagctcctt
     9421 gtctgatgcc ttcttcagcc tatttacggc gtacgatctc actgaaaggt ttttaaaagt
     9481 tgagcctaat agttctaatg cgtcgtctat atcaatttct gcccatgagt ccattagttc
     9541 taaaacctct actctttccg attcttctct caaatttgta ctctgtaata actttgtaag
     9601 agctttttta ttgttcatca aataatacct atatttccat attgaaccct tttcatgtgc
     9661 tgttaattta gtgccagggg ggtagttgat gattttattc aagtagtccc tctttttaat
     9721 gtccggcttc acttgcttgt ccaagtttgc atttttagat gctctttcca atctcctata
     9781 cttttcctct attggatcat tgtttggttg gtcaggatcg taaaacttca atgtggagtg
     9841 atatttgtcc cccaaagaaa ttttgatttg agggtcattc ctattcggtt ctcgtaagcc
     9901 tgtgcttagt ccaggattgt tcttcaaagt tggaatattc atttgagtat tcataatttc
     9961 cctttcgatg aacacaacag gaagctctaa cattggaaat tctaagttta agacaaaggt
    10021 gccctgaggc caagtcctat tttctcgttg tttccttaat ttgcttatag tgatttcgtc
    10081 aagccaaggg agtctcgtga attcaccctg aaaatattta ttcaaatttt cttgatcttt
    10141 gttatcagtg accacttcac agtggtcaat cacatcataa cgaaatttta aggattcaaa
    10201 ccctcttttt aaagtacagt cttttaagtt aaagatgctc gtttctagat tgaaaaaggg
    10261 aatttgcttg gaaccattgt attcccataa aataatgcgt aaatgactgc taaaggtaag
    10321 ctgtttgata cgtatgggca aagtcaacca ataatcccag gttctagagt ttctgaaagg
    10381 aatatacggc gtgtatatcg gaagagttaa atttctgttt ctctctttat caaatacctg
    10441 taaggacaca attagatcac tagaagggaa tacatttgac cctatcagca ttaattcagg
    10501 gttcaggatt ttttgagatg gcttcaacag tggcttatgt ccttccaatg acttgatttt
    10561 cactttcagg ggaacatcca gatcttgtga gacacagaat gtgatgttgt tcagagccat
    10621 ggtttattta tgtgtgttta ttcgaaacta agttcttggt gttttaaaac taaaaaaaag
    10681 actaactata aaagtagaat tcaagaagtt taagaaatag atttacagaa ttacaatcaa
    10741 tacctaccgt ctttatatac ttattagtca agtaggggaa taatttcagg gaactggttt
    10801 aaaccttttt tttcagtcga gatatctggt tgttaatttg attcgttcat ttgaggtttg
    10861 tggggccagg ttactgccaa ttttcctctt cataaccata aaagctagta ttgtagaatc
    10921 tttattgttc ggagcagtgc ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga
    10981 agacgaggac gcacggagga gagtcttcct tcggagggct gtcacccgct cggcggcttc
    11041 taatccgtac gcatgctttt tttggatctt tgcaaagatc cgcggccggt ccggggccgc
    11101 cagatcgatc ttcgagggga attctcttag gattcgattc acattcatct ttttttagct
    11161 actaatagga taaattatag gaatttataa caaattgaaa tatggcaggc agcaaaatta
    11221 aaattgtctt agttttttag tgtatagaag tgaatagcta tataaagtat gtgtaaagtt
    11281 ggtaacggaa cgaaaaatag aaaaggatat tacatgggaa aacatgttgt ttacggagaa
    11341 atgaaaagta tattgtattt tgtacgagct aaaagtacag tgggaacaaa gtcgacttaa
    11401 gcttgggtta ggatccgttt ccgctgatgg tcttatcatt gtgattcact gtagggctta
    11461 aggtaattga cttggatagt aaattggaag aaaacgctaa caaccacttt acattggtta
    11521 ataaaaattt catagccgtt gtccattcaa ggttgggact gctaccatgc aatttgacgg
    11581 ataatccatt aatcttgtcc ttattcatga tatagggtag ctcgagaatg gaagtactat
    11641 tttcaacgtc atttgcgtta ttcattgaag attcatcttg gcttgttgcg agtgtttgag
    11701 atgaatatga tgacgctatc gtagaaagct gccgagtgat ttcgcttatt atttctaatg
    11761 ttgtttctaa tgatttatca aacttcgttt ccttgcggaa gattctcccc aaattgaaat
    11821 tctcatcata gtagacaggc agaatcaacc aatcaccggg agcgttcgta gtggaattat
    11881 tatattcgac actgttaacc attcttttct tgattttgga aaatgaaccc atcggttgta
    11941 attcgtagtc cactaaattt atcttcaggt ttttgtttat ggtagccagt aaaagaatca
    12001 attggcccag tgctgcattg atttccttcc aaggaaccac tgattctggt atactgccta
    12061 atctcaatcc atttatagtg gcaaaggggc cgctatgtga gattttgaat gtagcattga
    12121 aaatattgat tttcctcaat ttgtctaatt gattcaagga taattcgtat tgcagtttta
    12181 atgactgtaa atttttgttg aattgaatgt tatttaaatc cattaaattc tgatcactca
    12241 gtttttggag tttctcattt tccagttgaa ctttcttttc ttgtaaacga actagttctc
    12301 catctaagtc atcatctgtc atttccaaac gtaataattg atccaagagt ctttcctctt
    12361 cctttttcaa attttctttt tccgaaagat tatgggaata ctgcttttct ttattgcttt
    12421 ctgatatttc tttgttttga ctctctaact tcgataaaaa ttgagcgtaa gtatctctct
    12481 cttttattgc atcatcatac tcactcttta gtctgtttat caagatgtta cagcagtcct
    12541 gacagatagg aaagtctatg tttgtttgag aagatagaat gttaaataca ttagtcattg
    12601 cgttgacttg tgtggacaaa gtcttggaat tcaattgtat ttgttggttt tcgtcttcat
    12661 ccgtagcttc ttgttcctct tcgtcatgat ctcgaaaggt actggttcca tcggaagtat
    12721 cactattagc ttttttatta tcatttccgt tggcatttcc ataacgctgg tcctctctgc
    12781 tattactcgt aatattagca ttatcatcat catcgtgatt aagaaaaaca aacgaatcag
    12841 tgatcaattt gtcatctatt aaattcaagt cttttatgtt ctgaatctcc cctaatcgcc
    12901 tcagcctctc cttcggtatt tgaggcccac aattatctgc ggcttcgatg cccttgttgc
    12961 tgatgacatt ttcattcgtt gcagtgataa ttgaattatt tgaaagtagc aaatttcttt
    13021 gggttaaact aagaccttct aaagatgggt ccagttgcag cggcaggtga catgtttggc
    13081 acttcatgcc catggcttcg tcgtgttgag ccaaagcagc agtcttcaat tcaccctcga
    13141 gtaactcacc ttggaagtac aagttttcag aagcagtggt cttcaactcg ccttgaaagt
    13201 acaaattttc agaggcagta gttggtctag aagatccgcc tccctggaaa tacaggtttt
    13261 ccgcgtctac tttcggcgcc tgagcatcat ttagcttttt agcttctgct aaaaggttag
    13321 cgctttggct tgggtcatct tttaaacttt ggatgaaggc gtttcgttgt tcttcgttta
    13381 agttaggtaa atgtaagatc tcatagaacg cgttttgttg ttctttgttg aatttgttgt
    13441 ctactttcgg cgcctgagca tcatttagct ttttagcttc tgctaaaagg ttagcgcttt
    13501 ggcttgggtc atcttttaaa ctttggatga aggcgtttcg ttgttcttcg tttaagttag
    13561 gtaaatgtaa gatctcatag aacgcgtttt gttgttcttt gttgaatttg ttgtctacgg
    13621 cttcatcgtg ggcgccctgg aagtacaggt tctcgctagc acccgcccct gctcccatca
    13681 tggtttattt atgtgtgttt attcgaaact aagttcttgg tgttttaaaa ctaaaaaaaa
    13741 gactaactat aaaagtagaa ttcaagaagt ttaagaaata gatttacaga attacaatca
    13801 atacctaccg tctttatata cttattagtc aagtagggga ataatttcag ggaactggtt
    13861 taaacctttt ttttcagtcg agatatctgg ttgttaattt gattcgttca tttgaggttt
    13921 gtggggccag gttactgcca attttcctct tcataaccat aaaagctagt attgtagaat
    13981 ctttattgtt cggagcagtg cggcgcgagg cacatctgcg tttcaggaac gcgaccggtg
    14041 aagacgagga cgcacggagg agagtcttcc ttcggagggc tgtcacccgc tcggcggctt
    14101 ctaatccgta cgcatgcttt ttttggatct ttgcaaagat ccgcggccct taattaaggg
    14161 ccgccagatc gatcttcgag gggaattctc ttaggattcg attcacattc atcttttttt
    14221 agctactaat aggataaatt ataggaattt ataacaaatt gaaatatggc aggcagcaaa
    14281 attaaaattg tcttagtttt ttagtgtata gaagtgaata gctatataaa gtatgtgtaa
    14341 agttggtaac ggaacgaaaa atagaaaagg atattacatg ggaaaacatg ttgtttacgg
    14401 agaaatgaaa agtatattgt attttgtacg agctaaaagt acagtgggaa caaagtcgac
    14461 ttaagcttgg gttaggatcc tgttggacgg taattctcca gaatctgttt aaattgcaat
    14521 atatcagcat taaaataatg tatggcttta atcagctttg cttgatgctt agaaatcatt
    14581 tgatcagtga aatacaacgg atattgactg tcgatcactg atgtactacc ataatacatt
    14641 agtctatagg gtaaaggagc cttgaaaatt tggatggctg tcaaatggag aaataaaagg
    14701 taataaccca actggctgtt tacatattct ctgtgctttg tggattcgcc ggcttgtcta
    14761 agtaatatct ctatattcaa aggttttagc ttgagcctat taataacatc atttacgcta
    14821 gaaggttttt caaagtaaat atagcctcta tctgaattaa ataaatccgt tgaatggaag
    14881 atccctatca gctgatacag tttcttaaac ctcagttggt ccaacctatc ttttatttgc
    14941 acaaatctag aatatatgct tccatagtca tcatttatag actcgtcctg cgacatcaga
    15001 gacagttctg ttttagaatg gttgatagta tcattactct cgagttgcgc catttctata
    15061 tttttcttgc tttttttcct ttgtaggact tccttttcta gcgtttctat ggacttctgt
    15121 actgttctca tataccaatg attttgattc ttgagtgaca aaaagtcatc ttctattttg
    15181 ctggaaattt cattgatttc ctcatggact tgggaagaat actccaatag tttgtttaat
    15241 tttagtaaag agctatatgc caatgaaaat ttgatcttga cttgacttat attccttttg
    15301 tgagaactaa caaggccctt tagtggctta attttttctg cagccaatgt gtaggatcca
    15361 tctattaaat caagtactgg tgcgttggtg cctgtaatta gaactgtgtc ttcatttatg
    15421 ggttgtaact tgttaagatc tatcgtgtag gtacataaaa cgcaccattt atcgtcaatg
    15481 ataccatttt tatctgaact tatggtacac aaaatatcac tcggaactag tcctaccaat
    15541 tttaggacaa tcatagtaga tgcaccagtt agttttggta attcctgaaa agagagttta
    15601 cgcaaagaac ctgattgtac ctcgctcaca taaagcagct cccctcttat ggattccaag
    15661 ataaagaaac atggtataaa tccatcacca tattccttga tcaatttgga gtcaccatta
    15721 gctctaaaga gacttatatt gtgaaagcaa atcatacgaa ggtgtctctg tcttcgactg
    15781 agtaagaacc gtttcatgcc catggtttat ttatgtgtgt ttattcgaaa ctaagttctt
    15841 ggtgttttaa aactaaaaaa aagactaact ataaaagtag aattcaagaa gtttaagaaa
    15901 tagatttaca gaattacaat caatacctac cgtctttata tacttattag tcaagtaggg
    15961 gaataatttc agggaactgg tttaaacctt ttttttcagt cgagatatct ggttgttaat
    16021 ttgattcgtt catttgaggt ttgtggggcc aggttactgc caattttcct cttcataacc
    16081 ataaaagcta gtattgtaga atctttattg ttcggagcag tgcggcgcga ggcacatctg
    16141 cgtttcagga acgcgaccgg tgaagacgag gacgcacgga ggagagtctt ccttcggagg
    16201 gctgtcaccc gctcggcggc ttctaatccg tacgcatgct ttttttggat ctttgcaaag
    16261 atccgcggcc cggcgcgcct gcaggacctg gtggcctata tggccaaaga gcctgctttt
    16321 ttgtacaaac ttgtgatgat ccgcgcccga tggtgggacg ggggccgcca ccgcggtgga
    16381 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
    16441 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata ggagccggaa
    16501 gcataaagtg taaagcctgg ggtgcctaat gagtgaggta actcacatta attgcgttgc
    16561 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    16621 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    16681 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    16741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    16801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    16861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    16921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    16981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    17041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    17101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    17161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    17221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    17281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    17341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    17401 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    17461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    17521 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    17581 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    17641 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    17701 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    17761 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    17821 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    17881 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    17941 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    18001 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    18061 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    18121 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    18181 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    18241 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    18301 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    18361 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    18421 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    18481 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    18541 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgaa cgaagcatct
    18601 gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat
    18661 ctgagctgca tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga
    18721 atctgtgctt catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa
    18781 agaatctgag ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac
    18841 aaagaatcta tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta
    18901 acaaagcatc ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat
    18961 aactttttgc actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct
    19021 cttccataaa aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg
    19081 gtgcattttt tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca
    19141 tactttgtga acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac
    19201 ggtttcttct attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt
    19261 tttcgattca ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag
    19321 agataaacat aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg
    19381 atgggtaggt tatataggga tatagcacag agatatatag caaagagata cttttgagca
    19441 atgtttgtgg aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt
    19501 ggttttttga aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct
    19561 atactttcta gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc
    19621 gcttccgaaa atgcaacgcg agctgcgcac atacagctca ctgttcacgt cgcacctata
    19681 tctgcgtgtt gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct
    19741 taaatgcgta cttatatgcg tctatttatg taggatgaaa ggtagtctag tacctcctgt
    19801 gatattatcc cattccatgc ggggtatcgt atgcttcctt cagcactacc ctttagctgt
    19861 tctatatgct gccactcctc aattggatta gtctcatcct tcaatgctat catttccttt
    19921 gatattggat catactaaga aaccattatt atcatgacat taacctataa aaataggcgt
    19981 atcacgaggc cctttcgtc
//
