LOCUS       Exported               10289 bp ds-DNA     circular SYN 31-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10289)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10289
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      complement(1483..1789)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(1811..1879)
                     /label=LacZ alpha
     primer_bind     1950..1967
                     /label=M13-fwd
     primer_bind     1976..1996
                     /label=T7
     misc_feature    2010..2037
                     /label=GAL-GAPDH promoter
     misc_feature    2047..2507
                     /label=GAL-TDH3 promoter
     misc_feature    2514..3836
                     /label=ScVPS38 untagged
     misc_feature    3837..3839
                     /label=stop
                     /label=stop(1)
     misc_feature    3851..4149
                     /label=MF (ALPHA)1 terminator
     misc_feature    4212..4672
                     /label=GAL-TDH3 promoter
                     /label=GAL-TDH3 promoter(1)
     misc_feature    4679..6349
                     /label=ScVPS30 coding
     misc_feature    4700
                     /label=mutation
                     /label=mutation(2)
     misc_feature    4702
                     /label=mutation
                     /label=mutation(7)
     misc_feature    4705
                     /label=mutation
                     /label=mutation(3)
     misc_feature    4862
                     /label=mutation
                     /label=mutation(6)
     misc_feature    5386
                     /label=mutation
                     /label=mutation(8)
     misc_feature    5394
                     /label=mutation
                     /label=mutation(4)
     misc_feature    5443
                     /label=mutation
                     /label=mutation(9)
     misc_feature    5731
                     /label=mutation
                     /label=mutation(5)
     misc_feature    5761
                     /label=mutation
                     /label=mutation(1)
     misc_feature    5776
                     /label=mutation
     misc_feature    6356..6358
                     /label=stop
     misc_feature    6370..6668
                     /label=MF (ALPHA)1 terminator
                     /label=MF (ALPHA)1 terminator(1)
     primer_bind     complement(6685..6704)
                     /label=T3
     primer_bind     complement(6722..6742)
                     /label=M13-rev
     misc_binding    complement(6748..6770)
                     /label=LacO
     rep_origin      complement(7110..7792)
                     /direction=LEFT
                     /label=ColE1 origin
     misc_feature    7172..7190
                     /label=YO032
     CDS             complement(7890..8549)
                     /label=AmpR
     misc_recomb     complement(9842..9875)
                     /label=FRT
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtaccg ctatgacatg attacgaatt ggcgcgccgg
     2041 ccggccggat ctttgcaaag atccaaaaaa agcatgcgta cggattagaa gccgccgagc
     2101 gggtgacagc cctccgaagg aagactctcc tccgtgcgtc ctcgtcttca ccggtcgcgt
     2161 tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa caataaagat tctacaatac
     2221 tagcttttat ggttatgaag aggaaaattg gcagtaacct ggccccacaa acctcaaatg
     2281 aacgaatcaa attaacaacc agatatctcg actgaaaaaa aaggtttaaa ccagttccct
     2341 gaaattattc ccctacttga ctaataagta tataaagacg gtaggtattg attgtaattc
     2401 tgtaaatcta tttcttaaac ttcttgaatt ctacttttat agttagtctt ttttttagtt
     2461 ttaaaacacc aagaacttag tttcgaataa acacacataa ataaaccatg ggcatgaaac
     2521 ggttcttact cagtcgaaga cagagacacc ttcgtatgat ttgctttcac aatataagtc
     2581 tctttagagc taatggtgac tccaaattga tcaaggaata tggtgatgga tttataccat
     2641 gtttctttat cttggaatcc ataagagggg agctgcttta tgtgagcgag gtacaatcag
     2701 gttctttgcg taaactctct tttcaggaat taccaaaact aactggtgca tctactatga
     2761 ttgtcctaaa attggtagga ctagttccga gtgatatttt gtgtaccata agttcagata
     2821 aaaatggtat cattgacgat aaatggtgcg ttttatgtac ctacacgata gatcttaaca
     2881 agttacaacc cataaatgaa gacacagttc taattacagg caccaacgca ccagtacttg
     2941 atttaataga tggatcctac acattggctg cagaaaaaat taagccacta aagggccttg
     3001 ttagttctca caaaaggaat ataagtcaag tcaagatcaa attttcattg gcatatagct
     3061 ctttactaaa attaaacaaa ctattggagt attcttccca agtccatgag gaaatcaatg
     3121 aaatttccag caaaatagaa gatgactttt tgtcactcaa gaatcaaaat cattggtata
     3181 tgagaacagt acagaagtcc atagaaacgc tagaaaagga agtcctacaa aggaaaaaaa
     3241 gcaagaaaaa tatagaaatg gcgcaactcg agagtaatga tactatcaac cattctaaaa
     3301 cagaactgtc tctgatgtcg caggacgagt ctataaatga tgactatgga agcatatatt
     3361 ctagatttgt gcaaataaaa gataggttgg accaactgag gtttaagaaa ctgtatcagc
     3421 tgatagggat cttccattca acggatttat ttaattcaga tagaggctat atttactttg
     3481 aaaaaccttc tagcgtaaat gatgttatta ataggctcaa gctaaaacct ttgaatatag
     3541 agatattact tagacaagcc ggcgaatcca caaagcacag agaatatgta aacagccagt
     3601 tgggttatta ccttttattt ctccatttga cagccatcca aattttcaag gctcctttac
     3661 cctatagact aatgtattat ggtagtacat cagtgatcga cagtcaatat ccgttgtatt
     3721 tcactgatca aatgatttct aagcatcaag caaagctgat taaagccata cattatttta
     3781 atgctgatat attgcaattt aaacagattc tggagaatta ccgtccaaca ggatcctaac
     3841 ccaagcttaa gtcgactttg ttcccactgt acttttagct cgtacaaaat acaatatact
     3901 tttcatttct ccgtaaacaa catgttttcc catgtaatat ccttttctat ttttcgttcc
     3961 gttaccaact ttacacatac tttatatagc tattcacttc tatacactaa aaaactaaga
     4021 caattttaat tttgctgcct gccatatttc aatttgttat aaattcctat aatttatcct
     4081 attagtagct aaaaaaagat gaatgtgaat cgaatcctaa gagaattccc ctcgaagatc
     4141 gatctggcgg cccttaatta acggaccgag cttggcactg gccgtcgttt acaacgtgcg
     4201 gccgcggccg cggatctttg caaagatcca aaaaaagcat gcgtacggat tagaagccgc
     4261 cgagcgggtg acagccctcc gaaggaagac tctcctccgt gcgtcctcgt cttcaccggt
     4321 cgcgttcctg aaacgcagat gtgcctcgcg ccgcactgct ccgaacaata aagattctac
     4381 aatactagct tttatggtta tgaagaggaa aattggcagt aacctggccc cacaaacctc
     4441 aaatgaacga atcaaattaa caaccagata tctcgactga aaaaaaaggt ttaaaccagt
     4501 tccctgaaat tattccccta cttgactaat aagtatataa agacggtagg tattgattgt
     4561 aattctgtaa atctatttct taaacttctt gaattctact tttatagtta gtcttttttt
     4621 tagttttaaa acaccaagaa cttagtttcg aataaacaca cataaataaa ccatgggcat
     4681 gaagtgccaa acatgtcacc tgccgctgca actggaccca tctttagaag gtcttagttt
     4741 aacccaaaga aatttgctac tttcaaataa ttcaattatc actgcaacga atgaaaatgt
     4801 catcagcaac aagggcatcg aagccgcaga taattgtggg cctcaaatac cgaaggagag
     4861 gctgaggcga ttaggggaga ttcagaacat aaaagacttg aatttaatag atgacaaatt
     4921 gatcactgat tcgtttgttt ttcttaatca cgatgatgat gataatgcta atattacgag
     4981 taatagcaga gaggaccagc gttatggaaa tgccaacgga aatgataata aaaaagctaa
     5041 tagtgatact tccgatggaa ccagtacctt tcgagatcat gacgaagagg aacaagaagc
     5101 tacggatgaa gacgaaaacc aacaaataca attgaattcc aagactttgt ccacacaagt
     5161 caacgcaatg actaatgtat ttaacattct atcttctcaa acaaacatag actttcctat
     5221 ctgtcaggac tgctgtaaca tcttgataaa cagactaaag agtgagtatg atgatgcaat
     5281 aaaagagaga gatacttacg ctcaattttt atcgaagtta gagagtcaaa acaaagaaat
     5341 atcagaaagc aataaagaaa agcagtattc ccataatctt tcggaaaaag aaaatttgaa
     5401 aaaggaagag gaaagactct tggatcaatt attacgtttg gaaatgacag atgatgactt
     5461 agatggagaa ctagttcgtt tacaagaaaa gaaagttcaa ctggaaaatg agaaactcca
     5521 aaaactgagt gatcagaatt taatggattt aaataacatt caattcaaca aaaatttaca
     5581 gtcattaaaa ctgcaatacg aattatcctt gaatcaatta gacaaattga ggaaaatcaa
     5641 tattttcaat gctacattca aaatctcaca tagcggcccc tttgccacta taaatggatt
     5701 gagattaggc agtataccag aatcagtggt tccttggaag gaaatcaatg cagcactggg
     5761 ccaattgatt cttttactgg ctaccataaa caaaaacctg aagataaatt tagtggacta
     5821 cgaattacaa ccgatgggtt cattttccaa aatcaagaaa agaatggtta acagtgtcga
     5881 atataataat tccactacga acgctcccgg tgattggttg attctgcctg tctactatga
     5941 tgagaatttc aatttgggga gaatcttccg caaggaaacg aagtttgata aatcattaga
     6001 aacaacatta gaaataataa gcgaaatcac tcggcagctt tctacgatag cgtcatcata
     6061 ttcatctcaa acactcgcaa caagccaaga tgaatcttca atgaataacg caaatgacgt
     6121 tgaaaatagt acttccattc tcgagctacc ctatatcatg aataaggaca agattaatgg
     6181 attatccgtc aaattgcatg gtagcagtcc caaccttgaa tggacaacgg ctatgaaatt
     6241 tttattaacc aatgtaaagt ggttgttagc gttttcttcc aatttactat ccaagtcaat
     6301 taccttaagc cctacagtga atcacaatga taagaccatc agcggaaacg gatcctaacc
     6361 caagcttaag tcgactttgt tcccactgta cttttagctc gtacaaaata caatatactt
     6421 ttcatttctc cgtaaacaac atgttttccc atgtaatatc cttttctatt tttcgttccg
     6481 ttaccaactt tacacatact ttatatagct attcacttct atacactaaa aaactaagac
     6541 aattttaatt ttgctgcctg ccatatttca atttgttata aattcctata atttatccta
     6601 ttagtagcta aaaaaagatg aatgtgaatc gaatcctaag agaattcccc tcgaagatcg
     6661 atctggcgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     6721 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6781 ggagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgaggta actcacatta
     6841 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6901 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6961 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     7021 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     7081 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     7141 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     7201 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     7261 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     7321 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     7381 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     7441 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     7501 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     7561 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7621 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7681 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7741 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7801 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7861 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7921 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7981 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     8041 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     8101 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     8161 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     8221 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     8281 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     8341 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     8401 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     8461 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     8521 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     8581 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8641 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8701 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8761 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8821 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgaa
     8881 cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc taatttttca
     8941 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag cgctatttta
     9001 ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgag agcgctaatt
     9061 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gagagcgcta
     9121 ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc ccgagagcgc
     9181 tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct ctataatgca
     9241 gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc tactttggtg
     9301 tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact gattactagc
     9361 gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct ataccgatgt
     9421 ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca ttggtcagaa
     9481 aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa tgtttacatt
     9541 ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt tgtctaaaga
     9601 gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag
     9661 cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag caaagagata
     9721 cttttgagca atgtttgtgg aagcggtatt cgcaatattt tagtagctcg ttacagtccg
     9781 gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc aaaagcgctc
     9841 tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca aagcgtttcc
     9901 gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca ctgttcacgt
     9961 cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg gcatagtgcg
    10021 tgtttatgct taaatgcgta cttatatgcg tctatttatg taggatgaaa ggtagtctag
    10081 tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt cagcactacc
    10141 ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct tcaatgctat
    10201 catttccttt gatattggat catactaaga aaccattatt atcatgacat taacctataa
    10261 aaataggcgt atcacgaggc cctttcgtc
//
