LOCUS       Exported                7050 bp ds-DNA     circular SYN 31-AUG-2017
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      complement(1483..1789)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(1811..1879)
                     /label=LacZ alpha
     primer_bind     1950..1967
                     /label=M13-fwd
     primer_bind     1976..1996
                     /label=T7
     misc_feature    2016..2476
                     /label=GAL-TDH3 promoter
     misc_feature    2483..3127
                     /label=ScVPS30 coding
     misc_feature    2504
                     /label=mutation
     misc_feature    2506
                     /label=mutation
                     /label=mutation(3)
     misc_feature    2509
                     /label=mutation
                     /label=mutation(1)
     misc_feature    2666
                     /label=mutation
                     /label=mutation(2)
     misc_feature    3131..3429
                     /label=MF (ALPHA)1 terminator
     primer_bind     complement(3446..3465)
                     /label=T3
     primer_bind     complement(3483..3503)
                     /label=M13-rev
     misc_binding    complement(3509..3531)
                     /label=LacO
     rep_origin      complement(3871..4553)
                     /direction=LEFT
                     /label=ColE1 origin
     misc_feature    3933..3951
                     /label=YO032
     CDS             complement(4651..5310)
                     /label=AmpR
     misc_recomb     complement(6603..6636)
                     /label=FRT
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtaccg gccgcggatc tttgcaaaga tccaaaaaaa
     2041 gcatgcgtac ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct
     2101 ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac
     2161 tgctccgaac aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaattgg
     2221 cagtaacctg gccccacaaa cctcaaatga acgaatcaaa ttaacaacca gatatctcga
     2281 ctgaaaaaaa aggtttaaac cagttccctg aaattattcc cctacttgac taataagtat
     2341 ataaagacgg taggtattga ttgtaattct gtaaatctat ttcttaaact tcttgaattc
     2401 tacttttata gttagtcttt tttttagttt taaaacacca agaacttagt ttcgaataaa
     2461 cacacataaa taaaccatgg gcatgaagtg ccaaacatgt cacctgccgc tgcaactgga
     2521 cccatcttta gaaggtctta gtttaaccca aagaaatttg ctactttcaa ataattcaat
     2581 tatcactgca acgaatgaaa atgtcatcag caacaagggc atcgaagccg cagataattg
     2641 tgggcctcaa ataccgaagg agaggctgag gcgattaggg gagattcaga acataaaaga
     2701 cttgaattta atagatgaca aattgatcac tgattcgttt gtttttctta atcacgatga
     2761 tgatgataat gctaatatta cgagtaatag cagagaggac cagcgttatg gaaatgccaa
     2821 cggaaatgat aataaaaaag ctaatagtga tacttccgat ggaaccagta cctttcgaga
     2881 tcatgacgaa gaggaacaag aagctacgga tgaagacgaa aaccaacaaa tacaattgaa
     2941 ttccaagact ttgtccacac aagtcaacgc aatgactaat gtatttaaca ttctatcttc
     3001 tcaaacaaac atagactttc ctatctgtca ggactgctgt aacatcttga taaacagact
     3061 aaagagtgag tatgatgatg caataaaaga gagagatact tacgctcaat ttttatcgaa
     3121 gttagagtaa gtcgactttg ttcccactgt acttttagct cgtacaaaat acaatatact
     3181 tttcatttct ccgtaaacaa catgttttcc catgtaatat ccttttctat ttttcgttcc
     3241 gttaccaact ttacacatac tttatatagc tattcacttc tatacactaa aaaactaaga
     3301 caattttaat tttgctgcct gccatatttc aatttgttat aaattcctat aatttatcct
     3361 attagtagct aaaaaaagat gaatgtgaat cgaatcctaa gagaattccc ctcgaagatc
     3421 gatctggcgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     3481 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     3541 aggagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt
     3601 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     3661 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     3721 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3781 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3841 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3901 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3961 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4021 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4081 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4141 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4201 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4261 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4321 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4381 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4441 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4501 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4561 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4621 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4681 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4741 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4801 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4861 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4921 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4981 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5041 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5101 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5161 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5221 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5281 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     5341 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     5401 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     5461 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     5521 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5581 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     5641 acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc
     5701 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt
     5761 accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat
     5821 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct
     5881 attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg
     5941 ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc
     6001 agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt
     6061 gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag
     6121 cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg
     6181 tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga
     6241 aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat
     6301 tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag
     6361 agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga
     6421 gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat
     6481 acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc
     6541 ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct
     6601 ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc aaagcgtttc
     6661 cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg
     6721 tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc
     6781 gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta
     6841 gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac
     6901 cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta
     6961 tcatttcctt tgatattgga tcatactaag aaaccattat tatcatgaca ttaacctata
     7021 aaaataggcg tatcacgagg ccctttcgtc
//
