LOCUS       Exported                7967 bp ds-DNA     circular SYN 12-DEC-2012
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7967
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      complement(1483..1789)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(1811..1879)
                     /label=LacZ alpha
     primer_bind     1950..1967
                     /label=M13-fwd
     primer_bind     1976..1996
                     /label=T7
     misc_feature    2010..4848
                     /label=VPS34 pro-coding
     misc_feature    2734..2751
                     /label=YO793
     misc_feature    3548..3565
                     /label=YO794
     misc_feature    4855..4920
                     /label=x3 FLAG
     misc_feature    4924..5156
                     /label=ADH1 terminator
     primer_bind     complement(5191..5210)
                     /label=T3
     primer_bind     complement(5228..5248)
                     /label=M13-rev
     misc_binding    complement(5254..5276)
                     /label=LacO
     rep_origin      complement(5616..6298)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6396..7055)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtaccg gggctgaagc tttcgacaaa tcttaaattg
     2041 tgactttgtg catgaaattt ttatatgaac gttttaaata agtggacata gtccttcatt
     2101 cggtatatgc agatccggca tcaaacactt tttcaagaaa gtttgagtca aaaggattaa
     2161 aggataaagt aaaacaacaa taacatctcc gtgaagcatt gagggaaggg tttaactcca
     2221 acaatgtcac tgaacaacat aacattctgt gtctcacagg atctggatgt tcccctgaaa
     2281 gtgaaaatca agtcattgga aggacataag ccactgttga agccatctca aaaaatcctg
     2341 aaccctgaat taatgctgat agggtcaaat gtattccctt ctagtgatct aattgtatcc
     2401 ttacaggtat ttgataaaga gagaaacaga aatttaactc ttccgatata cacgccgtat
     2461 attcctttca gaaactctag aacctgggat tattggttga ctttgcccat acgtatcaaa
     2521 cagcttacct ttagcagtca tttacgcatt attttgtggg aatacaatgg atccaagcaa
     2581 attccctttt tcaatctaga aacgagcatc tttaacttaa aagactgtac tttaaaaaga
     2641 gggtttgaat ccttaaaatt tcgttatgat gtgattgacc actgtgaagt ggtcactgat
     2701 aacaaagatc aagaaaattt gaataaatat tttcagggtg aattcacgag actcccttgg
     2761 cttgacgaaa tcactataag caaattaagg aaacaacggg aaaataggac ttggcctcag
     2821 ggcacctttg tcttaaactt agaatttcca atgttagagc ttcctgttgt gttcatcgaa
     2881 agggaaatta tgaatactca aatgaatatt ccaactttga agaacaatcc tggactaagc
     2941 acagacttac gagaaccgaa taggaatgat cctcaaatca aaatttcttt gggggacaaa
     3001 tatcactcca cattgaagtt ttacgatcct gaccaaccaa acaatgatcc aatagaggaa
     3061 aagtatagga gattggaaag agcatctaaa aatgcaaact tggacaagca agtgaagccg
     3121 gacattaaaa agagggacta cttgaataaa atcatcaact acccccctgg cactaaatta
     3181 acagcacatg aaaagggttc aatatggaaa tataggtatt atttgatgaa caataaaaaa
     3241 gctcttacaa agttattaca gagtacaaat ttgagagaag aatcggaaag agtagaggtt
     3301 ttagaactaa tggactcatg ggcagaaatt gatatagacg acgcattaga actattaggc
     3361 tcaactttta aaaacctttc agtgagatcg tacgccgtaa ataggctgaa gaaggcatca
     3421 gacaaggagc tggaattgta cttattacag ttggtagaag ctgtctgttt tgagaacctg
     3481 tccacttttt ccgacaaatc taacagtgaa ttcactattg tggatgccgt atcttcgcaa
     3541 aagctttcgg gtgattctat gttactatct acatcgcatg ccaaccaaaa attattaaag
     3601 tccatctcga gtgaatcgga aacttccggg acagaatcgc taccaatcgt catttctccc
     3661 ttggctgagt ttttgattag gagagcactg gtcaacccaa ggttaggaag ttttttctat
     3721 tggtatttaa agtccgagtc tgaggacaag ccatatttag accagatttt aagttccttt
     3781 tggagtagac tggataaaaa atctcggaat atactgaacg atcaagtcag gctaataaat
     3841 gtgctcaggg aatgttgtga aacaattaag aggctgaaag atactacagc caaaaagatg
     3901 gaattactag tgcatttatt ggagacgaaa gtcaggcctc ttgtcaaagt acgaccaatt
     3961 gctttaccac tagaccctga tgtgttgata tgcgacgttt gtcctgaaac ttcgaaggta
     4021 tttaaaagct ctttatcgcc actaaaaata acattcaaaa cgaccttaaa ccaaccatat
     4081 cacttaatgt ttaaggttgg cgatgatttg agacaagatc aactggtagt acagatcata
     4141 agtttaatga atgagttatt aaaaaatgaa aacgtggact tgaaattgac accatacaaa
     4201 attttggcaa caggaccaca agaaggtgcc attgaattta tccctaatga cacattagct
     4261 agcatactaa gcaaatatca cggtattctt ggctacctta aactccacta tcctgatgag
     4321 aacgcgacat taggcgtaca aggctgggtt ttagataatt ttgtcaaatc ttgtgctggc
     4381 tattgtgtta ttacatacat cttaggtgtc ggcgataggc atttagacaa cttactagtc
     4441 acgccagatg ggcacttttt tcacgcagac tttggttata tcttgggtca ggaccccaaa
     4501 ccttttccgc cattaatgaa attgccccct caaattatag aggcgtttgg gggtgcagaa
     4561 tcatcaaatt atgataaatt tcgcagctac tgttttgtgg catattcgat tttaagaagg
     4621 aatgcaggct taatcttaaa cctgtttgaa ttaatgaaga cttcgaacat acctgatatc
     4681 agaatagatc ccaatggtgc tatattacgt gtaagagaga gatttaattt gaatatgtcc
     4741 gaagaagatg ccacagtgca ttttcagaat ctaatcaatg atagtgtaaa tgctttgctg
     4801 cctatcgtga ttgatcattt acataatctg gcacaatact ggcggaccgt cgaggactac
     4861 aaagaccatg acggtgatta taaagatcat gatatcgatt acaaggatga cgatgacaag
     4921 tagaattcgt gagctcgcga atttcttatg atttatgatt tttattatta aataagttat
     4981 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt
     5041 attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc
     5101 tcttattgac cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg ctccccgcgg
     5161 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     5221 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5281 aacataggag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc
     5341 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5401 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     5461 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     5521 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5821 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6061 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6301 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6361 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6421 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6481 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6541 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6601 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6661 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     6721 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6781 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6841 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6901 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6961 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7021 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7081 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7141 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7201 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7261 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7321 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7381 cctgggtcct tttcatcacg tgctataaaa ataattataa tttaaatttt ttaatataaa
     7441 tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc
     7501 cgaagatgta aaagactcta gggggatcgc caacaaatac taccttttat cttgctcttc
     7561 ctgctctcag gtattaatgc cgaattgttt catcttgtct gtgtagaaga ccacacacga
     7621 aaatcctgtg attttacatt ttacttatcg ttaatcgaat gtatatctat ttaatctgct
     7681 tttcttgtct aataaatata tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt
     7741 gtttattttt ttttcttcat tccgtaactc ttctaccttc tttatttact ttctaaaatc
     7801 caaatacaaa acataaaaat aaataaacac agagtaaatt cccaaattat tccatcatta
     7861 aaagatacga ggcgcgtgta agttacaggc aagcgatccg tcctaagaaa ccattattat
     7921 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
