LOCUS       Exported                7360 bp ds-DNA     circular SYN 11-AUG-2014
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      complement(1483..1789)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(1811..1879)
                     /label=LacZ alpha
     primer_bind     1950..1967
                     /label=M13-fwd
     primer_bind     1976..1996
                     /label=T7
     misc_feature    2010..2943
                     /label=VPS38 promoter
     misc_feature    2955..4271
                     /label=VPS38 coding
     misc_feature    4278..4343
                     /label=x3 FLAG
     misc_feature    4344..4549
                     /label=ADH1 terminator
     primer_bind     complement(4584..4603)
                     /label=T3
     primer_bind     complement(4621..4641)
                     /label=M13-rev
     misc_binding    complement(4647..4669)
                     /label=LacO
     rep_origin      complement(5009..5691)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(5789..6448)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtaccg cgttgtgaga gagtgtgctc cttggctaga
     2041 cacttaggtt ccttgtttag tgacgccgtt caaactgcat ccgttcaatg gttcgaaaga
     2101 actctggatg attctgctat tagaagaatt tctttaccaa gtgcattttt aaccgcagat
     2161 attctattat ctactttgtt gaacatctca tccggtttag ttgtgtatcc aaaggttatc
     2221 gaaaggagaa ttaagggtga actacctttt atggctactg aaaatatcat catggctatg
     2281 gtagaaaaga atgcctccag acaagaagta catgagcgta ttagagtgct ctctcatcaa
     2341 gccgcagcag tagtcaagga agaaggtggg gaaaatgatt taattgaacg agtaaagagg
     2401 gatgaatttt tcaagcctat ctgggaagaa ttagattctt tactggaacc atccactttt
     2461 gttggtagag ctccacaaca agttgagaaa tttgttcaaa aagacgttaa caatgcttta
     2521 caacctttcc aaaagtacct aaacgatgaa caagtcaagt taaatgttta gaagccttta
     2581 catgtacaaa taatatgttt attaccctat tatatgttac taatgaaaaa aataacaatt
     2641 ctttatttat gtacaatgca agtagaattc cctaaataga agttaactat ttttatttta
     2701 ctgggatata tttttcatct tcttccttca tactcctttc atttttcttg tttttttggc
     2761 ttacggattt tggcaatgtt tatactttag aaaaaaagga aactcataat gctggcatac
     2821 ttgaaaaacc atacggaaag atcgtagttt atttttgtaa taagacactg aagtcagctg
     2881 tttgaaagag tatattgaat tgatggtttt acctattagg gatagtaatc ataatttaaa
     2941 aatgtttcat gggcatgaaa cggttcttac tcagtcgaag acagagacac cttcgtatga
     3001 tttgctttca caatataagt ctctttagag ctaatggtga ctccaaattg atcaaggaat
     3061 atggtgatgg atttatacca tgtttcttta tcttggaatc cataagaggg gagctgcttt
     3121 atgtgagcga ggtacaatca ggttctttgc gtaaactctc ttttcaggaa ttaccaaaac
     3181 taactggtgc atctactatg attgtcctaa aattggtagg actagttccg agtgatattt
     3241 tgtgtaccat aagttcagat aaaaatggta tcattgacga taaatggtgc gttttatgta
     3301 cctacacgat agatcttaac aagttacaac ccataaatga agacacagtt ctaattacag
     3361 gcaccaacgc accagtactt gatttaatag atggatccta cacattggct gcagaaaaaa
     3421 ttaagccact aaagggcctt gttagttctc acaaaaggaa tataagtcaa gtcaagatca
     3481 aattttcatt ggcatatagc tctttactaa aattaaacaa actattggag tattcttccc
     3541 aagtccatga ggaaatcaat gaaatttcca gcaaaataga agatgacttt ttgtcactca
     3601 agaatcaaaa tcattggtat atgagaacag tacagaagtc catagaaacg ctagaaaagg
     3661 aagtcctaca aaggaaaaaa agcaagaaaa atatagaaat ggcgcaactc gagagtaatg
     3721 atactatcaa ccattctaaa acagaactgt ctctgatgtc gcaggacgag tctataaatg
     3781 atgactatgg aagcatatat tctagatttg tgcaaataaa agataggttg gaccaactga
     3841 ggtttaagaa actgtatcag ctgataggga tcttccattc aacggattta tttaattcag
     3901 atagaggcta tatttacttt gaaaaacctt ctagcgtaaa tgatgttatt aataggctca
     3961 agctaaaacc tttgaatata gagatattac ttagacaagc cggcgaatcc acaaagcaca
     4021 gagaatatgt aaacagccag ttgggttatt accttttatt tctccatttg acagccatcc
     4081 aaattttcaa ggctccttta ccctatagac taatgtatta tggtagtaca tcagtgatcg
     4141 acagtcaata tccgttgtat ttcactgatc aaatgatttc taagcatcaa gcaaagctga
     4201 ttaaagccat acattatttt aatgctgata tattgcaatt taaacagatt ctggagaatt
     4261 accgtccaac agtcgaggac tacaaagacc atgacggtga ttataaagat catgatatcg
     4321 attacaagga tgacgatgac aagtagaatt cgtgagctcg cgaatttctt atgatttatg
     4381 atttttatta ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct
     4441 taggttttaa aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt
     4501 tctcaggtat agcatgaggt cgctcttatt gaccacacct ctaccggcag cggccgccac
     4561 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     4621 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatag
     4681 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa
     4741 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     4801 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     4861 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4921 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5461 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5581 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5641 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5701 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     5761 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5821 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5881 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5941 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6001 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6061 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6121 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6181 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6241 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6301 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6361 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6421 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6481 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6541 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6601 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6661 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6721 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt
     6781 ccttttcatc acgtgctata aaaataatta taatttaaat tttttaatat aaatatataa
     6841 attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt ttccgaagat
     6901 gtaaaagact ctagggggat cgccaacaaa tactaccttt tatcttgctc ttcctgctct
     6961 caggtattaa tgccgaattg tttcatcttg tctgtgtaga agaccacaca cgaaaatcct
     7021 gtgattttac attttactta tcgttaatcg aatgtatatc tatttaatct gcttttcttg
     7081 tctaataaat atatatgtaa agtacgcttt ttgttgaaat tttttaaacc tttgtttatt
     7141 tttttttctt cattccgtaa ctcttctacc ttctttattt actttctaaa atccaaatac
     7201 aaaacataaa aataaataaa cacagagtaa attcccaaat tattccatca ttaaaagata
     7261 cgaggcgcgt gtaagttaca ggcaagcgat ccgtcctaag aaaccattat tatcatgaca
     7321 ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
