LOCUS       Exported                7644 bp ds-DNA     circular SYN 23-AUG-2014
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      complement(1483..1789)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(1811..1879)
                     /label=LacZ alpha
     primer_bind     1950..1967
                     /label=M13-fwd
     primer_bind     1976..1996
                     /label=T7
     misc_feature    2010..2943
                     /label=VPS38 promoter
     misc_feature    2955..3911
                     /label=vps38 delta BARA2
     misc_feature    3921..4634
                     /label=EGFP
     primer_bind     complement(4868..4887)
                     /label=T3
     primer_bind     complement(4905..4925)
                     /label=M13-rev
     misc_binding    complement(4931..4953)
                     /label=LacO
     rep_origin      complement(5293..5975)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6073..6732)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtaccg cgttgtgaga gagtgtgctc cttggctaga
     2041 cacttaggtt ccttgtttag tgacgccgtt caaactgcat ccgttcaatg gttcgaaaga
     2101 actctggatg attctgctat tagaagaatt tctttaccaa gtgcattttt aaccgcagat
     2161 attctattat ctactttgtt gaacatctca tccggtttag ttgtgtatcc aaaggttatc
     2221 gaaaggagaa ttaagggtga actacctttt atggctactg aaaatatcat catggctatg
     2281 gtagaaaaga atgcctccag acaagaagta catgagcgta ttagagtgct ctctcatcaa
     2341 gccgcagcag tagtcaagga agaaggtggg gaaaatgatt taattgaacg agtaaagagg
     2401 gatgaatttt tcaagcctat ctgggaagaa ttagattctt tactggaacc atccactttt
     2461 gttggtagag ctccacaaca agttgagaaa tttgttcaaa aagacgttaa caatgcttta
     2521 caacctttcc aaaagtacct aaacgatgaa caagtcaagt taaatgttta gaagccttta
     2581 catgtacaaa taatatgttt attaccctat tatatgttac taatgaaaaa aataacaatt
     2641 ctttatttat gtacaatgca agtagaattc cctaaataga agttaactat ttttatttta
     2701 ctgggatata tttttcatct tcttccttca tactcctttc atttttcttg tttttttggc
     2761 ttacggattt tggcaatgtt tatactttag aaaaaaagga aactcataat gctggcatac
     2821 ttgaaaaacc atacggaaag atcgtagttt atttttgtaa taagacactg aagtcagctg
     2881 tttgaaagag tatattgaat tgatggtttt acctattagg gatagtaatc ataatttaaa
     2941 aatgtttcat gggcatgaaa cggttcttac tcagtcgaag acagagacac cttcgtatga
     3001 tttgctttca caatataagt ctctttagag ctaatggtga ctccaaattg atcaaggaat
     3061 atggtgatgg atttatacca tgtttcttta tcttggaatc cataagaggg gagctgcttt
     3121 atgtgagcga ggtacaatca ggttctttgc gtaaactctc ttttcaggaa ttaccaaaac
     3181 taactggtgc atctactatg attgtcctaa aattggtagg actagttccg agtgatattt
     3241 tgtgtaccat aagttcagat aaaaatggta tcattgacga taaatggtgc gttttatgta
     3301 cctacacgat agatcttaac aagttacaac ccataaatga agacacagtt ctaattacag
     3361 gcaccaacgc accagtactt gatttaatag atggatccta cacattggct gcagaaaaaa
     3421 ttaagccact aaagggcctt gttagttctc acaaaaggaa tataagtcaa gtcaagatca
     3481 aattttcatt ggcatatagc tctttactaa aattaaacaa actattggag tattcttccc
     3541 aagtccatga ggaaatcaat gaaatttcca gcaaaataga agatgacttt ttgtcactca
     3601 agaatcaaaa tcattggtat atgagaacag tacagaagtc catagaaacg ctagaaaagg
     3661 aagtcctaca aaggaaaaaa agcaagaaaa atatagaaat ggcgcaactc gagagtaatg
     3721 atactatcaa ccattctaaa acagaactgt ctctgatgtc gcaggacgag tctataaatg
     3781 atgactatgg aagcatatat tctagatttg tgcaaataaa agataggttg gaccaactga
     3841 ggtttaagaa actgtatcag ctgataggga tcttccattc aacggattta tttaattcag
     3901 atagaggcta tgtcgagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3961 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     4021 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4081 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     4141 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4201 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     4261 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4321 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     4381 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     4441 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4501 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     4561 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4621 acgagctgta caagtaagag ctcgcgaatt tcttatgatt tatgattttt attattaaat
     4681 aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa
     4741 aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg
     4801 aggtcgctct tattgaccac acctctaccg gcagcggccg ccaccgcggt ggagctccag
     4861 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     4921 tcctgtgtga aattgttatc cgctcacaat tccacacaac ataggagccg gaagcataaa
     4981 gtgtaaagcc tggggtgcct aatgagtgag gtaactcaca ttaattgcgt tgcgctcact
     5041 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5101 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5161 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5221 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5281 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5341 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5401 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5461 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5521 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5581 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5641 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5701 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     5761 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5821 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5881 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5941 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6001 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6061 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6121 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6181 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6241 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6301 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6361 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6421 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6481 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     6541 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6601 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     6661 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     6721 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     6781 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     6841 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6901 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6961 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7021 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcctttt catcacgtgc
     7081 tataaaaata attataattt aaatttttta atataaatat ataaattaaa aatagaaagt
     7141 aaaaaaagaa attaaagaaa aaatagtttt tgttttccga agatgtaaaa gactctaggg
     7201 ggatcgccaa caaatactac cttttatctt gctcttcctg ctctcaggta ttaatgccga
     7261 attgtttcat cttgtctgtg tagaagacca cacacgaaaa tcctgtgatt ttacatttta
     7321 cttatcgtta atcgaatgta tatctattta atctgctttt cttgtctaat aaatatatat
     7381 gtaaagtacg ctttttgttg aaatttttta aacctttgtt tatttttttt tcttcattcc
     7441 gtaactcttc taccttcttt atttactttc taaaatccaa atacaaaaca taaaaataaa
     7501 taaacacaga gtaaattccc aaattattcc atcattaaaa gatacgaggc gcgtgtaagt
     7561 tacaggcaag cgatccgtcc taagaaacca ttattatcat gacattaacc tataaaaata
     7621 ggcgtatcac gaggcccttt cgtc
//
