LOCUS       Exported                7817 bp ds-DNA     circular SYN 18-MAY-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 4, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(1351..1806)
                     /direction=LEFT
                     /label=M13 origin
     rep_origin      complement(1483..1789)
                     /direction=LEFT
                     /label=F1 ori
     CDS             complement(1811..1879)
                     /label=LacZ alpha
     primer_bind     1950..1967
                     /label=M13-fwd
     primer_bind     1976..1996
                     /label=T7
     misc_feature    2010..2223
                     /label=VPS34 pro-coding
     misc_feature    2224..2691
                     /label=C2
     misc_feature    2314..2388
                     /label=CBR1 (long)
     misc_feature    2353..2376
                     /label=CBR1
     misc_feature    2875..2895
                     /label=yoO352
     misc_feature    2905..2937
                     /label=C2 helical helix
     misc_feature    2950..3651
                     /label=helical
     misc_feature    3398..3415
                     /label=YO794
     misc_feature    3928..4698
                     /label=kinase domain
     misc_feature    4264..4266
                     /label=D731
     misc_feature    4546..4575
                     /label=Ka10
     misc_feature    4705..4770
                     /label=x3 FLAG
     misc_feature    4774..5006
                     /label=ADH1 terminator
     primer_bind     complement(5041..5060)
                     /label=T3
     primer_bind     complement(5078..5098)
                     /label=M13-rev
     misc_binding    complement(5104..5126)
                     /label=LacO
     rep_origin      complement(5466..6148)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(6246..6905)
                     /label=AmpR
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
      241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
      301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
      361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
      421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
      481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
      541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
      601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
      661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
      721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
      781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
      841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
      901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
      961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
     1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
     1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
     1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
     1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
     1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
     1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
     1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     1981 acgactcact atagggcgaa ttgggtaccg gggctgaagc tttcgacaaa tcttaaattg
     2041 tgactttgtg catgaaattt ttatatgaac gttttaaata agtggacata gtccttcatt
     2101 cggtatatgc agatccggca tcaaacactt tttcaagaaa gtttgagtca aaaggattaa
     2161 aggataaagt aaaacaacaa taacatctcc gtgaagcatt gagggaaggg tttaactcca
     2221 acaatgtcac tgaacaacat aacattctgt gtctcacagg atctggatgt tcccctgaaa
     2281 gtgaaaatca agtcattgga aggacataag ccactgttga agccatctca aaaaatcctg
     2341 aaccctgaat taatgctgat agggtcaaat gtattccctt ctagtgatct aattgtatcc
     2401 ttacaggtat ttgataaaga gagaaacaga aatttaactc ttccgatata cacgccgtat
     2461 attcctttca gaaactctag aacctgggat tattggttga ctttgcccat acgtatcaaa
     2521 cagcttacct ttagcagtca tttacgcatt attttgtggg aatacaatgg atccaagcaa
     2581 attccctttt tcaatctaga aacgagcatc tttaacttaa aagactgtac tttaaaaaga
     2641 gggtttgaat ccttaaaatt tcgttatgat gtgattgacc acttaaactt agaatttcca
     2701 atgttagagc ttcctgttgt gttcatcgaa agggaaatta tgaatactca aatgaatatt
     2761 ccaactttga agaacaatcc tggactaagc acagacttac gagaaccgaa taggaatgat
     2821 cctcaaatca aaatttcttt gggggacaaa tatcactcca cattgaagtt ttacgatcct
     2881 gaccaaccaa acaatgatcc aatagaggaa aagtatagga gattggaaag agcatctaaa
     2941 aatgcaaact tggacaagca agtgaagccg gacattaaaa agagggacta cttgaataaa
     3001 atcatcaact acccccctgg cactaaatta acagcacatg aaaagggttc aatatggaaa
     3061 tataggtatt atttgatgaa caataaaaaa gctcttacaa agttattaca gagtacaaat
     3121 ttgagagaag aatcggaaag agtagaggtt ttagaactaa tggactcatg ggcagaaatt
     3181 gatatagacg acgcattaga actattaggc tcaactttta aaaacctttc agtgagatcg
     3241 tacgccgtaa ataggctgaa gaaggcatca gacaaggagc tggaattgta cttattacag
     3301 ttggtagaag ctgtctgttt tgagaacctg tccacttttt ccgacaaatc taacagtgaa
     3361 ttcactattg tggatgccgt atcttcgcaa aagctttcgg gtgattctat gttactatct
     3421 acatcgcatg ccaaccaaaa attattaaag tccatctcga gtgaatcgga aacttccggg
     3481 acagaatcgc taccaatcgt catttctccc ttggctgagt ttttgattag gagagcactg
     3541 gtcaacccaa ggttaggaag ttttttctat tggtatttaa agtccgagtc tgaggacaag
     3601 ccatatttag accagatttt aagttccttt tggagtagac tggataaaaa atctcggaat
     3661 atactgaacg atcaagtcag gctaataaat gtgctcaggg aatgttgtga aacaattaag
     3721 aggctgaaag atactacagc caaaaagatg gaattactag tgcatttatt ggagacgaaa
     3781 gtcaggcctc ttgtcaaagt acgaccaatt gctttaccac tagaccctga tgtgttgata
     3841 tgcgacgttt gtcctgaaac ttcgaaggta tttaaaagct ctttatcgcc actaaaaata
     3901 acattcaaaa cgaccttaaa ccaaccatat cacttaatgt ttaaggttgg cgatgatttg
     3961 agacaagatc aactggtagt acagatcata agtttaatga atgagttatt aaaaaatgaa
     4021 aacgtggact tgaaattgac accatacaaa attttggcaa caggaccaca agaaggtgcc
     4081 attgaattta tccctaatga cacattagct agcatactaa gcaaatatca cggtattctt
     4141 ggctacctta aactccacta tcctgatgag aacgcgacat taggcgtaca aggctgggtt
     4201 ttagataatt ttgtcaaatc ttgtgctggc tattgtgtta ttacatacat cttaggtgtc
     4261 ggcgataggc atttagacaa cttactagtc acgccagatg ggcacttttt tcacgcagac
     4321 tttggttata tcttgggtca ggaccccaaa ccttttccgc cattaatgaa attgccccct
     4381 caaattatag aggcgtttgg gggtgcagaa tcatcaaatt atgataaatt tcgcagctac
     4441 tgttttgtgg catattcgat tttaagaagg aatgcaggct taatcttaaa cctgtttgaa
     4501 ttaatgaaga cttcgaacat acctgatatc agaatagatc ccaatggtgc tatattacgt
     4561 gtaagagaga gatttaattt gaatatgtcc gaagaagatg ccacagtgca ttttcagaat
     4621 ctaatcaatg atagtgtaaa tgctttgctg cctatcgtga ttgatcattt acataatctg
     4681 gcacaatact ggcggaccgt cgaggactac aaagaccatg acggtgatta taaagatcat
     4741 gatatcgatt acaaggatga cgatgacaag tagaattcgt gagctcgcga atttcttatg
     4801 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
     4861 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag
     4921 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc
     4981 gagcaaatgc ctgcaaatcg ctccccgcgg ccgccaccgc ggtggagctc cagcttttgt
     5041 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     5101 tgaaattgtt atccgctcac aattccacac aacataggag ccggaagcat aaagtgtaaa
     5161 gcctggggtg cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct
     5221 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5281 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5341 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5401 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5461 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5521 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5581 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5641 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5701 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5761 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5821 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5881 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     5941 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6001 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6061 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6121 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6181 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6241 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6301 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6361 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6421 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6481 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6541 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6601 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6661 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6721 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6781 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6841 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6901 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6961 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7021 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7081 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7141 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7201 gttccgcgca catttccccg aaaagtgcca cctgggtcct tttcatcacg tgctataaaa
     7261 ataattataa tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa
     7321 gaaattaaag aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc
     7381 caacaaatac taccttttat cttgctcttc ctgctctcag gtattaatgc cgaattgttt
     7441 catcttgtct gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg
     7501 ttaatcgaat gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt
     7561 acgctttttg ttgaaatttt ttaaaccttt gtttattttt ttttcttcat tccgtaactc
     7621 ttctaccttc tttatttact ttctaaaatc caaatacaaa acataaaaat aaataaacac
     7681 agagtaaatt cccaaattat tccatcatta aaagatacga ggcgcgtgta agttacaggc
     7741 aagcgatccg tcctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     7801 cacgaggccc tttcgtc
//
