LOCUS       pYY62_new_P1_int        5818 bp DNA     circular SYN 13-DEC-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Chang Liu Lab
  TITLE     Direct Submission
  JOURNAL   Exported Mar 5, 2025 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..25)
                     /label=6156
                     /note="color: black; sequence: AGGTGGCACTTTTCGGGGAAATGTG; 
                     added: 2024-11-04"
     primer_bind     18..40
                     /label=8048
                     /note="color: black; sequence: TGCCACCTGACGTCTAAGAAACC; 
                     added: 2024-11-04"
     primer_bind     35..84
                     /label=H448
                     /note="color: black; sequence: 
                     gaaaccattattatcatgacattaacctataaaaataggcgtatcacgag; added: 
                     2024-11-04"
     primer_bind     complement(79..95)
                     /label=9386
                     /note="color: black; sequence: gacgaaagggcctcgtg; added: 
                     2024-11-04"
     primer_bind     complement(81..97)
                     /label=9385
                     /note="color: black; sequence: gagacgaaagggcctcg; added: 
                     2024-11-04"
     misc_feature    98
                     /label=G in source
                     /note="color: #84b0dc; direction: RIGHT"
     primer_bind     122..143
                     /label=1959
                     /note="color: black; sequence: AACCTCTGACACATGCAGCTCC; 
                     added: 2024-11-04"
     primer_bind     128..145
                     /label=K184
                     /note="color: black; sequence: TGACACATGCAGCTCCCG; added: 
                     2024-11-04"
     primer_bind     complement(136..188)
                     /label=7403
                     /note="color: black; sequence: 
                     ttttaaattaataatgtaattcatatattatagtactCGTCTCCGGGAGCTGC; 
                     added: 2024-11-04"
     primer_bind     complement(138..157)
                     /label=K181
                     /note="color: black; sequence: agTactCGTCTCCGGGAGCT; added:
                     2024-11-04"
     primer_bind     complement(140..157)
                     /label=A218
                     /note="color: black; sequence: agTactCGTCTCCGGGAG; added: 
                     2024-11-04"
     primer_bind     complement(145..186)
                     /label=J925
                     /note="color: black; sequence: 
                     ttaaattaataatgtaattcatatattatagtactCGTCTCC; added: 
                     2024-11-04"
     primer_bind     149..208
                     /label=9772
                     /note="color: black; sequence: 
                     ACGagtactataatatatgaattacattattaatttaaaaGATACTAGATGGTgcagca
                     g; added: 2024-11-04"
     primer_bind     complement(150..188)
                     /label=8782
                     /note="color: black; sequence: 
                     ttttaaattaataatgtaattcatatattatagtactCG; added: 2024-11-04"
     misc_feature    complement(155..364)
                     /label=US junk p1 int flank 4
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    155..188
                     /label=pGKL1/ORF1
                     /note="color: #ff9ccd"
     misc_feature    165..188
                     /label=UTR?
                     /note="color: #ff120e"
     CDS             165..188
                     /label=TP-Polymerase
                     /note="color: #993366; direction: RIGHT"
     misc_feature    189..364
                     /label=junk DNA from Kll
                     /note="color: #85dae9; direction: RIGHT"
     primer_bind     228..247
                     /label=H857
                     /note="color: black; sequence: GCCAGTATTGGCGTTTCCCC; added:
                     2024-11-04"
     primer_bind     complement(273..307)
                     /label=K458
                     /note="color: black; sequence: 
                     GCTCTTTGAAGAGGTCACTATCTATTTCAAAGTCG; added: 2024-11-04"
     primer_bind     273..307
                     /label=K459
                     /note="color: black; sequence: 
                     CGACTTTGAAATAGATAGTGACCTCTTCAAAGAGC; added: 2024-11-04"
     misc_feature    291
                     /label=remove BsaI site
                     /note="color: #a6acb3"
     primer_bind     complement(331..354)
                     /label=A606
                     /note="color: black; sequence: GGCTTCATCTAATGTGGACTCTTC; 
                     added: 2024-11-04"
     primer_bind     complement(336..415)
                     /label=J324
                     /note="color: black; sequence: 
                     AAAAGGAGTTTTTTAGTTACCTTAATTtgAATAAACGAAATAAAAACTCGCAATCCATA
                     ATGGCTTCATCTAATGTGGAC; added: 2024-11-04"
     primer_bind     complement(336..395)
                     /label=J241
                     /note="color: black; sequence: 
                     CTTAATTtgAATAAACGAAATAAAAACTCGCAATCCATAATGGCTTCATCTAATGTGGA
                     C; added: 2024-11-04"
     primer_bind     complement(338..396)
                     /label=L027
                     /note="color: black; sequence: 
                     CCTTAATTtgAATAAACGAAATAAAAACTCGCAATCCATAATGGCTTCATCTAATGTGG
                     ; added: 2024-11-04"
     primer_bind     complement(339..398)
                     /label=H802
                     /note="color: black; sequence: 
                     TACCTTAATTtgAATAAACGAAATAAAAACTCGCAATCCATAATGGCTTCATCTAATGT
                     G; added: 2024-11-04"
     primer_bind     complement(356..415)
                     /label=J243
                     /note="color: black; sequence: 
                     AAAAGGAGTTTTTTAGTTACCTTAATTtgAATAAACGAAATAAAAACTCGCAATCCATA
                     A; added: 2024-11-04"
     primer_bind     365..453
                     /label=L028
                     /note="color: black; sequence: 
                     GCGAGTTTTTATTTCGTTTATTcaAATTAAGGTAACTAAAAAACTCCTTTTgatgacct
                     atacataggaagatctatagaaacaaaaag; added: 2024-11-04"
     misc_feature    365..388
                     /label=attP_TP901
                     /note="color: #85dae9; direction: RIGHT"
     misc_feature    365..386
                     /label=attP_LH
                     /note="color: #d6b295; direction: RIGHT"
     primer_bind     complement(369..415)
                     /label=K783
                     /note="color: black; sequence: 
                     AAAAGGAGTTTTTTAGTTACCTTAATTtgAATAAACGAAATAAAAAC; added: 
                     2024-11-04"
     misc_feature    387..388
                     /label=TP901_dinuc
                     /note="color: #ffcc99"
     misc_feature    389..415
                     /label=attP_RH
                     /note="color: #d6b295; direction: RIGHT"
     misc_feature    389..415
                     /label=attP_TP901
                     /note="color: #85dae9; direction: RIGHT"
     primer_bind     391..450
                     /label=J238
                     /note="color: black; sequence: 
                     TTAAGGTAACTAAAAAACTCCTTTTgatgacctatacataggaagatctatagaaacaa
                     a; added: 2024-11-04"
     5'UTR           416..517
                     /label=pGAaa
                     /label=p10B2
                     /note="color: #b4abac"
     primer_bind     416..445
                     /label=A820
                     /note="color: black; sequence: 
                     GATGACCTATACATAGGAAGATCTATAGAA; added: 2024-11-04"
     primer_bind     416..438
                     /label=573
                     /note="color: black; sequence: GATGACCTATACATAGGAAGATC; 
                     added: 2024-11-04"
     primer_bind     417..435
                     /label=9378
                     /note="color: black; sequence: atgacctatacataggaag; added: 
                     2024-11-04"
     primer_bind     complement(421..480)
                     /label=8544
                     /note="color: black; sequence: 
                     tttttctgatatttgaaagttattaatctttttgtttctatagatcttcctatgtatag
                     g; added: 2024-11-04"
     primer_bind     423..453
                     /label=6798
                     /note="color: black; sequence: 
                     tatacataggaagatctatagaaacaaaaag; added: 2024-11-04"
     primer_bind     428..453
                     /label=9376
                     /note="color: black; sequence: ataggaagatctatagaaacaaaaag; 
                     added: 2024-11-04"
     primer_bind     431..475
                     /label=H561
                     /note="color: black; sequence: 
                     ggaagatctatagaaacaaaaagattaataactttcaaatatcag; added: 
                     2024-11-04"
     primer_bind     442..475
                     /label=5528
                     /note="color: black; sequence: 
                     agaaacaaaaagattaataactttcaaatatcag; added: 2024-11-04"
     primer_bind     443..502
                     /label=8545
                     /note="color: black; sequence: 
                     gaaacaaaaagattaataactttcaaatatcagaaaaatAtagaaaCatGtgataagct
                     c; added: 2024-11-04"
     primer_bind     444..473
                     /label=F349
                     /note="color: black; sequence: 
                     aaacaaaaagattaataactttcaaatatc; added: 2024-11-04"
     primer_bind     complement(447..476)
                     /label=5526
                     /note="color: black; sequence: 
                     tctgatatttgaaagttattaatctttttg; added: 2024-11-04"
     primer_bind     462..494
                     /label=8481
                     /note="color: black; sequence: 
                     ctttcaaatatcagaaaaatatagaaacatgtg; added: 2024-11-04"
     primer_bind     complement(462..494)
                     /label=8482
                     /note="color: black; sequence: 
                     cacatgtttctatatttttctgatatttgaaag; added: 2024-11-04"
     primer_bind     complement(462..478)
                     /label=6627
                     /note="color: black; sequence: TTTCTGATATTTGAAAG; added: 
                     2024-11-04"
     primer_bind     complement(466..516)
                     /label=G495
                     /note="color: black; sequence: 
                     TtttatatgtctatgagcttatcaCatGtttctatatttttctgatatttg; added:
                     2024-11-04"
     primer_bind     complement(466..515)
                     /label=9076
                     /note="color: black; sequence: 
                     tttaTatgtctatgagcttatcaCatGtttctaTatttttctgatatttg; added: 
                     2024-11-04"
     primer_bind     complement(473..517)
                     /label=K602
                     /note="color: black; sequence: 
                     TTtttatatgtctatgagcttatcaCatGtttctatatttttctg; added: 
                     2024-11-04"
     primer_bind     complement(473..517)
                     /label=L130
                     /note="color: black; sequence: 
                     tttttaTatgtctatgagcttatcacatgtttctatatttttctg; added: 
                     2024-11-04"
     primer_bind     complement(473..515)
                     /label=G151
                     /note="color: black; sequence: 
                     tttatatgtctatgagcttatcaCatGtttctatatttttctg; added: 
                     2024-11-04"
     primer_bind     477..508
                     /label=F973
                     /note="color: black; sequence: 
                     aaaatatagaaacatgtgataagctcatagac; added: 2024-11-04"
     primer_bind     complement(482..515)
                     /label=D064
                     /note="color: black; sequence: 
                     tttaTatgtctatgagcttatcacatgtttctat; added: 2024-11-04"
     primer_bind     485..544
                     /label=H720
                     /note="color: black; sequence: 
                     gaaacatgtgataagctcatagacatAtaaaaaTATGAGATTCCCATCTATCTTCACCG
                     C; added: 2024-11-04"
     primer_bind     complement(485..544)
                     /label=H721
                     /note="color: black; sequence: 
                     GCGGTGAAGATAGATGGGAATCTCATAtttttaTatgtctatgagcttatcacatgttt
                     c; added: 2024-11-04"
     primer_bind     485..508
                     /label=F926
                     /note="color: black; sequence: gaaacatgtgataagctcatagac; 
                     added: 2024-11-04"
     primer_bind     488..521
                     /label=L112
                     /note="color: black; sequence: 
                     aCatGtgataagctcatagacatataaaaaTATG; added: 2024-11-04"
     primer_bind     complement(489..515)
                     /label=E889
                     /note="color: black; sequence: tttaTatgtctatgagcttatcacatg;
                     added: 2024-11-04"
     misc_feature    519..776
                     /label=app8 leader
                     /note="color: #c7b0e3"
     primer_bind     519..543
                     /label=A52
                     /note="color: black; sequence: ATGAGATTCCCATCTATCTTCACCG; 
                     added: 2024-11-04"
     primer_bind     519..543
                     /label=C947
                     /note="color: black; sequence: ATGAGATTCCCATCTATCTTCACCG; 
                     added: 2024-11-04"
     primer_bind     519..542
                     /label=9834
                     /note="color: black; sequence: ATGAGATTCCCATCTATCTTCACC; 
                     added: 2024-11-04"
     primer_bind     complement(521..543)
                     /label=K584
                     /note="color: black; sequence: CGGTGAAGATAGATGGGAATCTC; 
                     added: 2024-11-04"
     primer_bind     567..583
                     /label=7652
                     /note="color: black; sequence: GCTTTGGCTGCTCCAGC; added: 
                     2024-11-04"
     primer_bind     664..683
                     /label=C689
                     /note="color: black; sequence: ACGCTGCTGCTTTGCCATTG; added:
                     2024-11-04"
     primer_bind     672..691
                     /label=K865
                     /note="color: black; sequence: GCTTTGCCATTGTCTAACTC; added:
                     2024-11-04"
     primer_bind     694..715
                     /label=E327
                     /note="color: black; sequence: CCAACAACGGTTTGTCTTCTAC; 
                     added: 2024-11-04"
     primer_bind     725..784
                     /label=J525
                     /note="color: black; sequence: 
                     CATCGCTTCTATCGCTGCTAAGGAAGAAGGTGTTCAATTGGACAAGAGAGAAGCTGACG
                     C; added: 2024-11-04"
     primer_bind     744..765
                     /label=A33
                     /note="color: black; sequence: AAGGAAGAAGGTGTTCAATTGG; 
                     added: 2024-11-04"
     primer_bind     complement(746..776)
                     /label=7241
                     /note="color: black; sequence: 
                     TTCTCTCTTGTCCAATTGAACACCTTCTTCC; added: 2024-11-04"
     primer_bind     complement(750..785)
                     /label=8368
                     /note="color: black; sequence: 
                     TGCGTCAGCTTCTCTCTTGTCCAATTGAACACCTTC; added: 2024-11-04"
     primer_bind     complement(750..775)
                     /label=C884
                     /note="color: black; sequence: TCTCTCTTGTCCAATTGAACACCTTC; 
                     added: 2024-11-04"
     primer_bind     complement(750..774)
                     /label=C883
                     /note="color: black; sequence: CTCTCTTGTCCAATTGAACACCTTC; 
                     added: 2024-11-04"
     primer_bind     750..772
                     /label=C688
                     /note="color: black; sequence: GAAGGTGTTCAATTGGACAAGAG; 
                     added: 2024-11-04"
     primer_bind     complement(752..777)
                     /label=C885
                     /note="color: black; sequence: CTTCTCTCTTGTCCAATTGAACACCT; 
                     added: 2024-11-04"
     primer_bind     complement(753..778)
                     /label=8896
                     /note="color: black; sequence: GCTTCTCTCTTGTCCAATTGAACACC; 
                     added: 2024-11-04"
     primer_bind     753..778
                     /label=9387
                     /note="color: black; sequence: GGTGTTCAATTGGACAAGAGAGAAGC; 
                     added: 2024-11-04"
     primer_bind     764..813
                     /label=H784
                     /note="color: black; sequence: 
                     GGACAAGAGAGAAGCTGACGCAgagaccgaaagtgaaacgtgatttcatg; added: 
                     2024-11-04"
     primer_bind     complement(764..813)
                     /label=H785
                     /note="color: black; sequence: 
                     catgaaatcacgtttcactttcggtctcTGCGTCAGCTTCTCTCTTGTCC; added: 
                     2024-11-04"
     primer_bind     complement(764..785)
                     /label=B580
                     /note="color: black; sequence: TGCGTCAGCTTCTCTCTTGTCC; 
                     added: 2024-11-04"
     primer_bind     764..785
                     /label=J187
                     /note="color: black; sequence: GGACAAGAGAGAAGCTGACGCA; 
                     added: 2024-11-04"
     primer_bind     complement(764..785)
                     /label=J190
                     /note="color: black; sequence: TGCGTCAGCTTCTCTCTTGTCC; 
                     added: 2024-11-04"
     primer_bind     764..783
                     /label=9638
                     /note="color: black; sequence: GGACAAGAGAGAAGCTGACG; added:
                     2024-11-04"
     primer_bind     766..813
                     /label=K585
                     /note="color: black; sequence: 
                     ACAAGAGAGAAGCTGACGCAgagaccgaaagtgaaacgtgatttcatg; added: 
                     2024-11-04"
     primer_bind     complement(769..785)
                     /label=J642
                     /note="color: black; sequence: TGCGTCAGCTTCTCTCT; added: 
                     2024-11-04"
     misc_feature    777..785
                     /label=peptide cleavage site
                     /note="color: #ffcc00"
     primer_bind     786..810
                     /label=L128
                     /note="color: black; sequence: gagaccgaaagtgaaacgtgatttc; 
                     added: 2024-11-04"
     primer_bind     786..805
                     /label=D036
                     /note="color: black; sequence: gagaccgaaagtgaaacgtg; added:
                     2024-11-04"
     misc_feature    786..791
                     /label=BsaI
                     /note="color: #ccffff"
     promoter        792..921
                     /label=BBa_J72163 GlpT Promoter
                     /note="color: #9eff91"
     primer_bind     792..814
                     /label=C979
                     /note="color: black; sequence: gaaagtgaaacgtgatttcatgc; 
                     added: 2024-11-04"
     primer_bind     792..814
                     /label=D103
                     /note="color: black; sequence: gaaagtgaaacgtgatttcatgc; 
                     added: 2024-11-04"
     RBS             922..957
                     /label=sfGFP Ribosome Binding Site
                     /note="color: #9eff91"
     CDS             958..1674
                     /label=sfGFP
                     /note="color: #34ff03; direction: RIGHT"
     primer_bind     958..983
                     /label=B879
                     /note="color: black; sequence: atgcgtaaaggcgaagagctgttcac; 
                     added: 2024-11-04"
     primer_bind     958..981
                     /label=C807
                     /note="color: black; sequence: atgcgtaaaggcgaagagctgttc; 
                     added: 2024-11-04"
     primer_bind     958..978
                     /label=2298
                     /note="color: black; sequence: atgcgtaaaggcgaagagctg; 
                     added: 2024-11-04"
     primer_bind     complement(969..991)
                     /label=5384
                     /note="color: black; sequence: cgacaccagtgaacagctcttcg; 
                     added: 2024-11-04"
     primer_bind     complement(973..992)
                     /label=2793
                     /note="color: black; sequence: acgacaccagtgaacagctc; added:
                     2024-11-04"
     primer_bind     complement(988..1007)
                     /label=2297
                     /note="color: black; sequence: tccaccagaatagggacgac; added:
                     2024-11-04"
     primer_bind     1066..1086
                     /label=4977
                     /note="color: black; sequence: gcaactaatggtaaactgacg; 
                     added: 2024-11-04"
     primer_bind     1069..1092
                     /label=5713
                     /note="color: black; sequence: actaatggtaaactgacgctgaag; 
                     added: 2024-11-04"
     primer_bind     1083..1106
                     /label=5511
                     /note="color: black; sequence: GACGCTGAAGTTCATCTGTACTAC; 
                     added: 2024-11-04"
     primer_bind     complement(1229..1247)
                     /label=5714
                     /note="color: black; sequence: gtgcgttcctgcacatagc; added: 
                     2024-11-04"
     primer_bind     complement(1268..1288)
                     /label=6160
                     /note="color: black; sequence: ccgcacgcgttttgtacgtgc; 
                     added: 2024-11-04"
     primer_bind     1404..1423
                     /label=5886
                     /note="color: black; sequence: tgtttacatcaccgccgata; added:
                     2024-11-04"
     primer_bind     1457..1478
                     /label=H317
                     /note="color: black; sequence: ttcgccacaacgtggaggatgg; 
                     added: 2024-11-04"
     primer_bind     1613..1632
                     /label=2889
                     /note="color: black; sequence: ttctgctggagttcgtaacc; added:
                     2024-11-04"
     primer_bind     complement(1638..1671)
                     /label=8977
                     /note="color: black; sequence: 
                     tttgtacagttcatccataccatgcgtgatgccc; added: 2024-11-04"
     primer_bind     complement(1644..1674)
                     /label=B880
                     /note="color: black; sequence: 
                     tcatttgtacagttcatccataccatgcgtg; added: 2024-11-04"
     primer_bind     complement(1646..1674)
                     /label=C808
                     /note="color: black; sequence: 
                     tcatttgtacagttcatccataccatgcg; added: 2024-11-04"
     primer_bind     complement(1646..1674)
                     /label=F733
                     /note="color: black; sequence: 
                     tcatttgtacagttcatccataccatgcg; added: 2024-11-04"
     primer_bind     complement(1647..1674)
                     /label=C802
                     /note="color: black; sequence: 
                     tcatttgtacagttcatccataccatgc; added: 2024-11-04"
     primer_bind     1650..1676
                     /label=H220
                     /note="color: black; sequence: tggtatggatgaactgtacaaatgacc;
                     added: 2024-11-04"
     primer_bind     complement(1652..1674)
                     /label=4512
                     /note="color: black; sequence: tcatttgtacagttcatccatac; 
                     added: 2024-11-04"
     terminator      1675..1803
                     /label=BBa_B0015 Terminator
                     /note="color: #ccffff"
     primer_bind     1785..1833
                     /label=H786
                     /note="color: black; sequence: 
                     gggcctttctgcgtttataggtctcGGTTCCaaggacaatagctcgacg; added: 
                     2024-11-04"
     primer_bind     complement(1785..1833)
                     /label=H787
                     /note="color: black; sequence: 
                     cgtcgagctattgtccttGGAACCgagacctataaacgcagaaaggccc; added: 
                     2024-11-04"
     primer_bind     complement(1786..1830)
                     /label=K586
                     /note="color: black; sequence: 
                     cgagctattgtccttGGAACCgagacctataaacgcagaaaggcc; added: 
                     2024-11-04"
     misc_feature    1804..1809
                     /label=BsaI
                     /note="color: #ccffff"
     primer_bind     complement(1810..1869)
                     /label=J526
                     /note="color: black; sequence: 
                     gtagtctggaacgtcgtatgggtatctaccttcaatcgtcgagctattgtccttGGAAC
                     C; added: 2024-11-04"
     primer_bind     complement(1810..1833)
                     /label=J188
                     /note="color: black; sequence: cgtcgagctattgtccttGGAACC; 
                     added: 2024-11-04"
     primer_bind     1810..1833
                     /label=J189
                     /note="color: black; sequence: GGTTCCaaggacaatagctcgacg; 
                     added: 2024-11-04"
     primer_bind     complement(1810..1831)
                     /label=B525
                     /note="color: black; sequence: tcgagctattgtccttGGAACC; 
                     added: 2024-11-04"
     primer_bind     1819..1837
                     /label=J641
                     /note="color: black; sequence: gacaatagctcgacgattg; added: 
                     2024-11-04"
     misc_feature    1836..1845
                     /label=Factor Xa
                     /note="color: #85dae9"
     primer_bind     complement(1840..1861)
                     /label=A34
                     /note="color: black; sequence: gaacgtcgtatgggtatctacc; 
                     added: 2024-11-04"
     CDS             1846..1872
                     /codon_start=1
                     /label=HA tag
                     /note="color: #84b0dc"
                     /translation="YPYDVPDYA"
     primer_bind     1857..1876
                     /label=K055
                     /note="color: black; sequence: cgttccagactacgctctgc; added:
                     2024-11-04"
     primer_bind     complement(1857..1876)
                     /label=K106
                     /note="color: black; sequence: gcagagcgtagtctggaacg; added:
                     2024-11-04"
     primer_bind     complement(1870..1890)
                     /label=4042
                     /note="color: black; sequence: accaccactagcctgcagagc; 
                     added: 2024-11-04"
     misc_feature    1885..1929
                     /label=(G4S)3
                     /note="color: #ff9ccd"
     primer_bind     1913..1934
                     /label=7667
                     /note="color: black; sequence: CTGGTGGTGGTGGTTCTGCTAG; 
                     added: 2024-11-04"
     primer_bind     complement(1918..1935)
                     /label=986
                     /note="color: black; sequence: GCTAGCAGAACCACCACC; added: 
                     2024-11-04"
     primer_bind     1921..1941
                     /label=K056
                     /note="color: black; sequence: ggtggttctgctagcgacgtc; 
                     added: 2024-11-04"
     primer_bind     complement(1924..1962)
                     /label=8229
                     /note="color: black; sequence: 
                     GCATATAGTTGTCAGTTCCTGgacgtcgctagcagaacc; added: 2024-11-04"
     primer_bind     1924..1962
                     /label=8230
                     /note="color: black; sequence: 
                     ggttctgctagcgacgtcCAGGAACTGACAACTATATGC; added: 2024-11-04"
     primer_bind     complement(1924..1940)
                     /label=7415
                     /note="color: black; sequence: acgtcgctagcagaacc; added: 
                     2024-11-04"
     primer_bind     complement(1934..1952)
                     /label=C309
                     /note="color: black; sequence: GTCAGTTCCTGgacgtcgc; added: 
                     2024-11-04"
     misc_feature    1942..2148
                     /label=Aga2p
                     /note="color: #b4abac"
     primer_bind     complement(1942..1963)
                     /label=K866
                     /note="color: black; sequence: CGCATATAGTTGTCAGTTCCTG; 
                     added: 2024-11-04"
     primer_bind     complement(1942..1963)
                     /label=K875
                     /note="color: black; sequence: CGCATATAGTTGTCAGTTCCTG; 
                     added: 2024-11-04"
     primer_bind     1945..1966
                     /label=8225
                     /note="color: black; sequence: GAACTGACAACTATATGCGAGC; 
                     added: 2024-11-04"
     primer_bind     2070..2091
                     /label=E807
                     /note="color: black; sequence: AGTAACGTTTGTCAGTAATTGC; 
                     added: 2024-11-04"
     primer_bind     2081..2101
                     /label=8034
                     /note="color: black; sequence: TCAGTAATTGCGGTTCTCACC; 
                     added: 2024-11-04"
     primer_bind     complement(2090..2105)
                     /label=A384
                     /note="color: black; sequence: GAGGGGTGAGAACCGC; added: 
                     2024-11-04"
     primer_bind     2092..2111
                     /label=932
                     /note="color: black; sequence: ggttctcacccctcaacaac; added:
                     2024-11-04"
     misc_feature    2152..2168
                     /label=Addition
                     /note="This feature has 2 segments:
                        1: 2152 .. 2164 / #a6acb3
                        2: 2165 .. 2168 / #a6acb3"
     misc_feature    2169..2243
                     /label=polyA
                     /note="color: #cc66fe"
     misc_feature    2244..2292
                     /label=HHRz
                     /label=Ribozyme
                     /note="color: #ffef86"
     primer_bind     complement(2244..2266)
                     /label=H932
                     /note="color: black; sequence: cggaaaacacatccggtgacagg; 
                     added: 2024-11-04"
     primer_bind     2257..2276
                     /label=L163
                     /note="color: black; sequence: GTGTTTTCCGGTCTGATGAG; added:
                     2024-11-04"
     primer_bind     2287..2346
                     /label=L131
                     /note="color: black; sequence: 
                     CGAAACaggactaggctgcgaatttcttatgatttatgatttttattattaaataagtt
                     a; added: 2024-11-04"
     misc_feature    2297..2304
                     /label=addition
                     /note="color: #a6acb3"
     primer_bind     2304..2361
                     /label=8192
                     /note="color: black; sequence: 
                     Gcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtg;
                      added: 2024-11-04"
     primer_bind     2304..2361
                     /label=J421
                     /note="color: black; sequence: 
                     Gcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtg;
                      added: 2024-11-04"
     primer_bind     2304..2344
                     /label=1586
                     /note="color: black; sequence: 
                     GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGT; added: 
                     2024-11-04"
     primer_bind     complement(2304..2344)
                     /label=2011
                     /note="color: black; sequence: 
                     ACTTATTTAATAATAAAAATCATAAATCATAAGAAATTCGC; added: 
                     2024-11-04"
     primer_bind     2304..2343
                     /label=1682
                     /note="color: black; sequence: 
                     GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAG; added: 
                     2024-11-04"
     primer_bind     2304..2343
                     /label=A302
                     /note="color: black; sequence: 
                     gcgaatttcttatgatttatgatttttattattaaataag; added: 
                     2024-11-04"
     primer_bind     2304..2324
                     /label=6124
                     /note="color: black; sequence: GCGAATTTCTTATGATTTATG; 
                     added: 2024-11-04"
     misc_feature    complement(2305..2469)
                     /label=T ADH1
                     /note="color: #b4abac; direction: LEFT"
     primer_bind     2305..2359
                     /label=1776
                     /note="color: black; sequence: 
                     CGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAG; 
                     added: 2024-11-04"
     primer_bind     2305..2343
                     /label=1781
                     /note="color: black; sequence: 
                     CGAATTTCTTATGATTTATGATTTTTATTATTAAATAAG; added: 2024-11-04"
     primer_bind     complement(2305..2343)
                     /label=D477
                     /note="color: black; sequence: 
                     cttatttaataataaaaatcataaatcataagaaattcg; added: 2024-11-04"
     primer_bind     2305..2340
                     /label=298
                     /note="color: black; sequence: 
                     CGAATTTCTTATGATTTATGATTTTTATTATTAAAT; added: 2024-11-04"
     primer_bind     2311..2361
                     /label=C897
                     /note="color: black; sequence: 
                     tcttatgatttatgatttttattattaaataagttataaaaaaaataagtg; added:
                     2024-11-04"
     primer_bind     complement(2334..2358)
                     /label=67
                     /note="color: black; sequence: TTATTTTTTTTATAACTTATTTAAT; 
                     added: 2024-11-04"
     primer_bind     complement(2355..2387)
                     /label=A581
                     /note="color: black; sequence: 
                     ctaagagtcactttaaaatttgtatacacttat; added: 2024-11-04"
     primer_bind     complement(2377..2411)
                     /label=2875
                     /note="color: black; sequence: 
                     gaataagaattttcgttttaaaacctaagagtcac; added: 2024-11-04"
     primer_bind     complement(2420..2442)
                     /label=2344
                     /note="color: black; sequence: GCAACCTGACCTACAGGAAAGAG; 
                     added: 2024-11-04"
     primer_bind     2430..2469
                     /label=A408
                     /note="color: black; sequence: 
                     taggtcaggttgctttctcaggtatagcatgaggtcgctc; added: 
                     2024-11-04"
     primer_bind     2430..2451
                     /label=B870
                     /note="color: black; sequence: taggtcaggttgctttctcagg; 
                     added: 2024-11-04"
     primer_bind     complement(2432..2450)
                     /label=8929
                     /note="color: black; sequence: ctgagaaagcaacctgacc; added: 
                     2024-11-04"
     primer_bind     2440..2493
                     /label=C496
                     /note="color: black; sequence: 
                     tgctttctcaggtatagcatgaggtcgctcttaagcaaggattttcttaacttc; 
                     added: 2024-11-04"
     primer_bind     complement(2440..2469)
                     /label=G233
                     /note="color: black; sequence: 
                     gagcgacctcatgctatacctgagaaagca; added: 2024-11-04"
     primer_bind     complement(2446..2499)
                     /label=K454
                     /note="color: black; sequence: 
                     gccgaagaagttaagaaaatccttgcttaagagcgacctcatgctatacctgag; 
                     added: 2024-11-04"
     primer_bind     2446..2499
                     /label=K455
                     /note="color: black; sequence: 
                     ctcaggtatagcatgaggtcgctcttaagcaaggattttcttaacttcttcggc; 
                     added: 2024-11-04"
     primer_bind     complement(2446..2469)
                     /label=3651
                     /note="color: black; sequence: gagcgacctcatgctatacctgag; 
                     added: 2024-11-04"
     primer_bind     complement(2446..2469)
                     /label=K423
                     /note="color: black; sequence: gagcgacctcatgctatacctgag; 
                     added: 2024-11-04"
     primer_bind     complement(2451..2469)
                     /label=9876
                     /note="color: black; sequence: gagcgacctcatgctatac; added: 
                     2024-11-04"
     CDS             complement(2470..3564)
                     /label=LEU2
                     /note="color: #ffff99; direction: LEFT"
     primer_bind     2470..2496
                     /label=A219
                     /note="color: black; sequence: ttaagcaaggattttcttaacttcttc;
                     added: 2024-11-04"
     primer_bind     2470..2496
                     /label=F399
                     /note="color: black; sequence: ttaagcaaggattttcttaacttcttc;
                     added: 2024-11-04"
     primer_bind     2471..2497
                     /label=6783
                     /note="color: black; sequence: taagcaaggattttcttaacttcttcg;
                     added: 2024-11-04"
     primer_bind     2475..2499
                     /label=K996
                     /note="color: black; sequence: caaggattttcttaacttcttcggc; 
                     added: 2024-11-04"
     primer_bind     complement(2487..2510)
                     /label=71
                     /note="color: black; sequence: gtgatgctgtcgccgaagaagtta; 
                     added: 2024-11-04"
     primer_bind     2521..2570
                     /label=9507
                     /note="color: black; sequence: 
                     ggtactgttggaaccacctaaatcaccagttctgatacctgcatccaaaa; added: 
                     2024-11-04"
     primer_bind     complement(2531..2580)
                     /label=9506
                     /note="color: black; sequence: 
                     gttaaaaaggttttggatgcaggtatcagaactggtgatttaggtggttc; added: 
                     2024-11-04"
     primer_bind     2674..2693
                     /label=193
                     /note="color: black; sequence: ATTCTTTGGCAAATCTGGAG; added:
                     2024-11-04"
     primer_bind     2746..2765
                     /label=54
                     /note="color: black; sequence: AGAGGCCAAGGACGCAGATG; added:
                     2024-11-04"
     primer_bind     2848..2867
                     /label=393
                     /note="color: black; sequence: ATTTAGGTGGGTTGGGTTCT; added:
                     2024-11-04"
     primer_bind     2848..2867
                     /label=5763
                     /note="color: black; sequence: ATTTAGGTGGGTTGGGTTCT; added:
                     2024-11-04"
     primer_bind     2848..2867
                     /label=E708
                     /note="color: black; sequence: ATTTAGGTGGGTTGGGTTCT; added:
                     2024-11-04"
     primer_bind     complement(2885..2907)
                     /label=333
                     /note="color: black; sequence: caacatcaattgattgattctgc; 
                     added: 2024-11-04"
     primer_bind     complement(2905..2937)
                     /label=3889
                     /note="color: black; sequence: 
                     atcaagaacgaatttCCTACATTGAAGGTTCAA; added: 2024-11-04"
     primer_bind     2909..2945
                     /label=3890
                     /note="color: black; sequence: 
                     accttcaatgtaggaAATTCGTTCTTGATGGTTTCCT; added: 2024-11-04"
     primer_bind     2927..2946
                     /label=1292
                     /note="color: black; sequence: TCGTTCTTGATGGTTTCCTC; added:
                     2024-11-04"
     primer_bind     2941..2974
                     /label=H481
                     /note="color: black; sequence: 
                     TTCCTCCACAGTTTTTCTCCATAATCTTGAAGAG; added: 2024-11-04"
     primer_bind     complement(2969..2988)
                     /label=392
                     /note="color: black; sequence: GCTAATGTTTTGGCCTCTTC; added:
                     2024-11-04"
     primer_bind     complement(2969..2988)
                     /label=5762
                     /note="color: black; sequence: GCTAATGTTTTGGCCTCTTC; added:
                     2024-11-04"
     primer_bind     complement(2969..2988)
                     /label=E707
                     /note="color: black; sequence: GCTAATGTTTTGGCCTCTTC; added:
                     2024-11-04"
     primer_bind     complement(2975..2994)
                     /label=6779
                     /note="color: black; sequence: GATAAAGCTAATGTTTTGGC; added:
                     2024-11-04"
     primer_bind     2975..2994
                     /label=6780
                     /note="color: black; sequence: GCCAAAACATTAGCTTTATC; added:
                     2024-11-04"
     primer_bind     2976..2996
                     /label=5466
                     /note="color: black; sequence: CCAAAACATTAGCTTTATCCA; 
                     added: 2024-11-04"
     primer_bind     complement(2976..2995)
                     /label=76
                     /note="color: black; sequence: GGATAAAGCTAATGTTTTGG; added:
                     2024-11-04"
     primer_bind     2988..3044
                     /label=1901
                     /note="color: black; sequence: 
                     ctttatccaaggaccaaataggcaatggtggctcatgttgtagggccatgaaagcgg; 
                     added: 2024-11-04"
     primer_bind     2988..3015
                     /label=8054
                     /note="color: black; sequence: 
                     ctttatccaaggaccaaataggcaatgg; added: 2024-11-04"
     primer_bind     complement(2988..3008)
                     /label=5467
                     /note="color: black; sequence: CTATTTGGTCCTTGGATAAAG; 
                     added: 2024-11-04"
     primer_bind     complement(3007..3066)
                     /label=1899
                     /note="color: black; sequence: 
                     gtgcaaagaatcacaagaatggccgctttcatggccctacaacatgagccaccattgcc
                     t; added: 2024-11-04"
     primer_bind     complement(3007..3026)
                     /label=19
                     /note="color: black; sequence: AACATGAGCCACCATTGCCT; added:
                     2024-11-04"
     primer_bind     complement(3008..3024)
                     /label=9760
                     /note="color: black; sequence: catgagccaccattgcc; added: 
                     2024-11-04"
     primer_bind     3014..3033
                     /label=J3
                     /note="color: black; sequence: ggtggctcatgttgtagggc; added:
                     2024-11-04"
     primer_bind     3028..3063
                     /label=220
                     /note="color: black; sequence: 
                     TAGGGCCATGAAAGCGGCCATTCTTGTGATTCTTTG; added: 2024-11-04"
     primer_bind     complement(3041..3064)
                     /label=H480
                     /note="color: black; sequence: GCAAAGAATCACAAGAATGGCCGC; 
                     added: 2024-11-04"
     primer_bind     3044..3063
                     /label=24
                     /note="color: black; sequence: GCCATTCTTGTGATTCTTTG; added:
                     2024-11-04"
     primer_bind     3088..3104
                     /label=A843
                     /note="color: black; sequence: actatcccaagcgacac; added: 
                     2024-11-04"
     primer_bind     complement(3178..3197)
                     /label=75
                     /note="color: black; sequence: AGCCACAATTTGCTAAAGGT; added:
                     2024-11-04"
     primer_bind     3199..3221
                     /label=8399
                     /note="color: black; sequence: GATTGGAGATAAGTCTAAAAGAG; 
                     added: 2024-11-04"
     primer_bind     3321..3340
                     /label=69
                     /note="color: black; sequence: ATTTAGGACCACCCACAGCA; added:
                     2024-11-04"
     primer_bind     complement(3382..3402)
                     /label=175
                     /note="color: black; sequence: GGTGTTCCACTTCCAGATGAG; 
                     added: 2024-11-04"
     primer_bind     3448..3466
                     /label=8400
                     /note="color: black; sequence: GAACTTGACATTGGAACGA; added: 
                     2024-11-04"
     primer_bind     3460..3480
                     /label=7339
                     /note="color: black; sequence: ggaacgaacatcagaaatagc; 
                     added: 2024-11-04"
     primer_bind     3506..3525
                     /label=6548
                     /note="color: black; sequence: gtgatttcttgaccaacgtg; added:
                     2024-11-04"
     primer_bind     complement(3535..3554)
                     /label=192
                     /note="color: black; sequence: CTAAGAAGATCGTCGTTTTG; added:
                     2024-11-04"
     primer_bind     complement(3554..3643)
                     /label=L029
                     /note="color: black; sequence: 
                     CGAGTTTTTATTTCGTTTATTTCAATTAAGGTAACTAAAAAACTCCTTTTTATATGAAA
                     GTTTTTATAATAATTATAAAatgtctgcccc; added: 2024-11-04"
     primer_bind     complement(3562..3593)
                     /label=8541
                     /note="color: black; sequence: 
                     tatatgaaagtttttataataattataaaatg; added: 2024-11-04"
     misc_feature    complement(3565..3593)
                     /label=UTR region from pGKL1 ORF2
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    3594..3620
                     /label=attP_RH
                     /note="color: #d6b295"
     misc_feature    3594..3620
                     /label=attP_TP901
                     /note="color: #85dae9"
     primer_bind     3607..3666
                     /label=H799
                     /note="color: black; sequence: 
                     TAGTTACCTTAATTGAAATAAACGAAATAAAAACTCGCcctcagactcagattggtgtt
                     t; added: 2024-11-04"
     primer_bind     3608..3667
                     /label=J240
                     /note="color: black; sequence: 
                     AGTTACCTTAATTGAAATAAACGAAATAAAAACTCGCcctcagactcagattggtgttt
                     c; added: 2024-11-04"
     primer_bind     3613..3671
                     /label=L030
                     /note="color: black; sequence: 
                     CCTTAATTGAAATAAACGAAATAAAAACTCGCcctcagactcagattggtgtttcaaag
                     ; added: 2024-11-04"
     primer_bind     complement(3617..3644)
                     /label=G167
                     /note="color: black; sequence: 
                     GCGAGTTTTTATTTCGTTTATTTCAATT; added: 2024-11-04"
     misc_feature    3621..3644
                     /label=attP_TP901
                     /note="color: #85dae9"
     misc_feature    3621..3622
                     /label=TP901_dinuc
                     /note="color: #d59687"
     misc_feature    3623..3644
                     /label=attP_LH
                     /note="color: #d6b295"
     misc_feature    complement(3645..3799)
                     /label=Recombination flank from ORF4 pGKL1
                     /note="color: #b1ff67; direction: LEFT"
     primer_bind     3645..3687
                     /label=7673
                     /note="color: black; sequence: 
                     cctcagactcagattggtgtttcaaagatcctcaaggcaatag; added: 
                     2024-11-04"
     primer_bind     3645..3667
                     /label=5257
                     /note="color: black; sequence: cctcagactcagattggtgtttc; 
                     added: 2024-11-04"
     primer_bind     complement(3645..3664)
                     /label=6852
                     /note="color: black; sequence: ACACCAATCTGAGTCTGAGG; added:
                     2024-11-04"
     primer_bind     3645..3663
                     /label=H724
                     /note="color: black; sequence: cctcagactcagattggtg; added: 
                     2024-11-04"
     primer_bind     complement(3648..3695)
                     /label=7670
                     /note="color: black; sequence: 
                     ctgttattctattgccttgaggatctttgaaacaccaatctgagtctg; added: 
                     2024-11-04"
     primer_bind     complement(3650..3671)
                     /label=G314
                     /note="color: black; sequence: ctttgaaacaccaatctgagtc; 
                     added: 2024-11-04"
     primer_bind     complement(3667..3700)
                     /label=F563
                     /note="color: black; sequence: 
                     aaaatctgttattctattgccttgaggatctttg; added: 2024-11-04"
     primer_bind     complement(3677..3706)
                     /label=443
                     /note="color: black; sequence: 
                     ACTATCAAAATCTGTTATTCTATTGCCTTG; added: 2024-11-04"
     primer_bind     complement(3677..3706)
                     /label=E893
                     /note="color: black; sequence: 
                     ACTATCAAAATCTGTTATTCTATTGCCTTG; added: 2024-11-04"
     primer_bind     complement(3682..3741)
                     /label=8389
                     /note="color: black; sequence: 
                     acatctcttctaccaagacctaattctttattaatactatcaaaatctgttattctatt
                     g; added: 2024-11-04"
     primer_bind     complement(3701..3745)
                     /label=H856
                     /note="color: black; sequence: 
                     ttttacatctcttctaccaagacctaattctttattaatactatc; added: 
                     2024-11-04"
     primer_bind     complement(3722..3781)
                     /label=8388
                     /note="color: black; sequence: 
                     acataatttaattaaatcatcatgacctttatctaattttacatctcttctaccaagac
                     c; added: 2024-11-04"
     primer_bind     complement(3722..3745)
                     /label=6851
                     /note="color: black; sequence: TTTTACATCTCTTCTACCAAGACC; 
                     added: 2024-11-04"
     primer_bind     complement(3722..3742)
                     /label=H723
                     /note="color: black; sequence: tacatctcttctaccaagacc; 
                     added: 2024-11-04"
     primer_bind     complement(3722..3741)
                     /label=H813
                     /note="color: black; sequence: acatctcttctaccaagacc; added:
                     2024-11-04"
     primer_bind     complement(3722..3741)
                     /label=L162
                     /note="color: black; sequence: acatctcttctaccaagacc; added:
                     2024-11-04"
     primer_bind     complement(3740..3799)
                     /label=A484
                     /note="color: black; sequence: 
                     ACTATCTATTTTTTCAGTACATAATTTAATTAAATCATCATGACCTTTATCTAATTTTA
                     C; added: 2024-11-04"
     primer_bind     complement(3749..3799)
                     /label=7394
                     /note="color: black; sequence: 
                     actatctattttttcagtacataatttaattaaatcatcatgacctttatc; added:
                     2024-11-04"
     primer_bind     complement(3755..3799)
                     /label=G118
                     /note="color: black; sequence: 
                     actatctattttttcagtacataatttaattaaatcatcatgacc; added: 
                     2024-11-04"
     primer_bind     complement(3755..3799)
                     /label=K956
                     /note="color: black; sequence: 
                     actatctattttttcagtacataatttaattaaatcatcatgacc; added: 
                     2024-11-04"
     primer_bind     complement(3764..3799)
                     /label=7921
                     /note="color: black; sequence: 
                     ACTATCTATTTTTTCAGTACATAATTTAATTAAATC; added: 2024-11-04"
     primer_bind     complement(3764..3799)
                     /label=8452
                     /note="color: black; sequence: 
                     ACTATCTATTTTTTCAGTACATAATTTAATTAAATC; added: 2024-11-04"
     primer_bind     complement(3764..3796)
                     /label=7313
                     /note="color: black; sequence: 
                     ATCTATTTTTTCAGTACATAATTTAATTAAATC; added: 2024-11-04"
     primer_bind     3765..3824
                     /label=7402
                     /note="color: black; sequence: 
                     atttaattaaattatgtactgaaaaaatagatagtactATTAATTGCGTTGCGCTCACT
                     G; added: 2024-11-04"
     primer_bind     3800..3818
                     /label=D322
                     /note="color: black; sequence: actATTAATTGCGTTGCGC; added: 
                     2024-11-04"
     primer_bind     3806..3825
                     /label=H355
                     /note="color: black; sequence: AATTGCGTTGCGCTCACTGC; added:
                     2024-11-04"
     primer_bind     complement(3845..3868)
                     /label=H410
                     /note="color: black; sequence: cattaatgcagctggcacgacagg; 
                     added: 2024-11-04"
     primer_bind     complement(3846..3862)
                     /label=A385
                     /note="color: black; sequence: tgcagctggcacgacag; added: 
                     2024-11-04"
     primer_bind     complement(3886..3906)
                     /label=8053
                     /note="color: black; sequence: CAATACGCAAACCGCCTCTCC; 
                     added: 2024-11-04"
     primer_bind     3886..3906
                     /label=K298
                     /note="color: black; sequence: ggagaggcggtttgcgtattg; 
                     added: 2024-11-04"
     primer_bind     3973..3998
                     /label=B667
                     /note="color: black; sequence: cagctcactcaaaggcggtaatacgg; 
                     added: 2024-11-04"
     primer_bind     3995..4016
                     /label=G761
                     /note="color: black; sequence: acggttatccacagaatcaggg; 
                     added: 2024-11-04"
     primer_bind     3995..4014
                     /label=G065
                     /note="color: black; sequence: acggttatccacagaatcag; added:
                     2024-11-04"
     primer_bind     complement(3996..4023)
                     /label=H933
                     /note="color: black; sequence: 
                     cgttatcccctgattctgtggataaccg; added: 2024-11-04"
     primer_bind     complement(3996..4015)
                     /label=J179
                     /note="color: black; sequence: cctgattctgtggataaccg; added:
                     2024-11-04"
     primer_bind     4006..4031
                     /label=B666
                     /note="color: black; sequence: cagaatcaggggataacgcaggaaag; 
                     added: 2024-11-04"
     primer_bind     complement(4006..4023)
                     /label=6755
                     /note="color: black; sequence: CGTTATCCCCTGATTCTG; added: 
                     2024-11-04"
     primer_bind     complement(4016..4042)
                     /label=H172
                     /note="color: black; sequence: gctcacatgttctttcctgcgttatcc;
                     added: 2024-11-04"
     primer_bind     4016..4042
                     /label=H175
                     /note="color: black; sequence: ggataacgcaggaaagaacatgtgagc;
                     added: 2024-11-04"
     primer_bind     4018..4042
                     /label=H313
                     /note="color: black; sequence: ATAACGCAGGAAAGAACATGTGAGC; 
                     added: 2024-11-04"
     primer_bind     complement(4039..4058)
                     /label=E870
                     /note="color: black; sequence: cttttgctggccttttgctc; added:
                     2024-11-04"
     primer_bind     4040..4060
                     /label=F924
                     /note="color: black; sequence: AGCAAAAGGCCAGCAAAAGGC; 
                     added: 2024-11-04"
     primer_bind     complement(4040..4060)
                     /label=F925
                     /note="color: black; sequence: GCCTTTTGCTGGCCTTTTGCT; 
                     added: 2024-11-04"
     primer_bind     complement(4041..4062)
                     /label=H270
                     /note="color: black; sequence: TGGCCTTTTGCTGGCCTTTTGC; 
                     added: 2024-11-04"
     primer_bind     complement(4049..4069)
                     /label=2744
                     /note="color: black; sequence: cggttcctggccttttgctgg; 
                     added: 2024-11-04"
     primer_bind     complement(4050..4067)
                     /label=D386
                     /note="color: black; sequence: GTTCCTGGCCTTTTGCTG; added: 
                     2024-11-04"
     primer_bind     complement(4060..4080)
                     /label=G210
                     /note="color: black; sequence: cggcctttttacggttcctgg; 
                     added: 2024-11-04"
     rep_origin      4076..4758
                     /label=ColE1 origin
                     /note="color: #9eafd2"
     primer_bind     4076..4098
                     /label=E500
                     /note="color: black; sequence: ggccgcgttgctggcgtttttcc; 
                     added: 2024-11-04"
     primer_bind     4076..4095
                     /label=E640
                     /note="color: black; sequence: GGCCGCGTTGCTGGCGTTTT; added:
                     2024-11-04"
     primer_bind     complement(4080..4099)
                     /label=E418
                     /note="color: black; sequence: tggaaaaacgccagcaacgc; added:
                     2024-11-04"
     primer_bind     complement(4089..4110)
                     /label=K804
                     /note="color: black; sequence: ggcggagcctatggaaaaacgc; 
                     added: 2024-11-04"
     rep_origin      4094..4682
                     /label=pMB1 origin of replication (counter-clockwise)(
                     /label=pMB1 origin of replication (counter-clockwise)(RNAII
                     -35 to RNA/DNA switch point)
                     /note="color: #9eafd2"
     misc_RNA        complement(4094..4646)
                     /label=RNAII transcript (complementary strand)
                     /note="color: #b4abac; direction: LEFT"
     primer_bind     complement(4113..4137)
                     /label=3469
                     /note="color: black; sequence: GTCGATTTTTGTGATGCTCGTCAGG; 
                     added: 2024-11-04"
     primer_bind     complement(4113..4135)
                     /label=3095
                     /note="color: black; sequence: CGATTTTTGTGATGCTCGTCAGG; 
                     added: 2024-11-04"
     primer_bind     complement(4114..4135)
                     /label=K395
                     /note="color: black; sequence: CGATTTTTGTGATGCTCGTCAG; 
                     added: 2024-11-04"
     primer_bind     complement(4116..4139)
                     /label=H995
                     /note="color: black; sequence: GCGTCGATTTTTGTGATGCTCGTC; 
                     added: 2024-11-04"
     primer_bind     complement(4134..4153)
                     /label=K132
                     /note="color: black; sequence: cacctctgacttgagcgtcg; added:
                     2024-11-04"
     primer_bind     4138..4161
                     /label=6980
                     /note="color: black; sequence: gctcaagtcagaggtggcgaaacc; 
                     added: 2024-11-04"
     primer_bind     complement(4138..4161)
                     /label=6981
                     /note="color: black; sequence: ggtttcgccacctctgacttgagc; 
                     added: 2024-11-04"
     primer_bind     complement(4144..4163)
                     /label=6878
                     /note="color: black; sequence: Cgggtttcgccacctctgac; added:
                     2024-11-04"
     primer_bind     4151..4168
                     /label=7564
                     /note="color: black; sequence: GTGGCGAAACCCGACAGG; added: 
                     2024-11-04"
     primer_bind     complement(4167..4191)
                     /label=H312
                     /note="color: black; sequence: GAAACGCCTGGTATCTTTATAGTCC; 
                     added: 2024-11-04"
     primer_bind     4171..4197
                     /label=H996
                     /note="color: black; sequence: TATAAAGATACCAGGCGTTTCCCCCTG;
                     added: 2024-11-04"
     primer_bind     complement(4214..4232)
                     /label=1215
                     /note="color: black; sequence: CAGGGTCGGAACAGGAGAG; added: 
                     2024-11-04"
     primer_bind     complement(4235..4254)
                     /label=3750
                     /note="color: black; sequence: CGGACAGGTATCCGGTAAGC; added:
                     2024-11-04"
     primer_bind     4291..4317
                     /label=3759
                     /note="color: black; sequence: GCTCACGCTGTAGGTATCTCAGTTCGG;
                     added: 2024-11-04"
     primer_bind     complement(4291..4317)
                     /label=3761
                     /note="color: black; sequence: CCGAACTGAGATACCTACAGCGTGAGC;
                     added: 2024-11-04"
     primer_bind     complement(4304..4337)
                     /label=2173
                     /note="color: black; sequence: 
                     CTTGGAGCGAACGACCTACACCGAACTGAGATAC; added: 2024-11-04"
     primer_bind     complement(4304..4333)
                     /label=2130
                     /note="color: black; sequence: 
                     gagcgaacgacctacaccgaactgagatac; added: 2024-11-04"
     primer_bind     4306..4330
                     /label=1960
                     /note="color: black; sequence: ATCTCAGTTCGGTGTAGGTCGTTCG; 
                     added: 2024-11-04"
     primer_bind     4352..4369
                     /label=H711
                     /note="color: black; sequence: CGAACCCCCCGTTCAGCC; added: 
                     2024-11-04"
     primer_bind     complement(4370..4388)
                     /label=H712
                     /note="color: black; sequence: GGATAAGGCGCAGCGGTCG; added: 
                     2024-11-04"
     primer_bind     4414..4435
                     /label=J950
                     /note="color: black; sequence: cggtaagacacgacttatcgcc; 
                     added: 2024-11-04"
     primer_bind     complement(4415..4438)
                     /label=J951
                     /note="color: black; sequence: agtggcgataagtcgtgtcttacc; 
                     added: 2024-11-04"
     primer_bind     4465..4484
                     /label=5485
                     /note="color: black; sequence: GCAGAGCGAGGTATGTAGGC; added:
                     2024-11-04"
     primer_bind     4471..4488
                     /label=1264
                     /note="color: black; sequence: CGAGGTATGTAGGCGGTG; added: 
                     2024-11-04"
     primer_bind     complement(4483..4502)
                     /label=5484
                     /note="color: black; sequence: CAAGAACTCTGTAGCACCGC; added:
                     2024-11-04"
     -35_signal      4500..4505
                     /label=RNAI promoter (clockwise)
                     /label=RNAI promoter (clockwise); TTGAAG
                     /note="color: #c6c9d1; direction: RIGHT"
     -10_signal      4522..4527
                     /label=RNAI promoter (clockwise)
                     /label=RNAI promoter (clockwise); GCTACA
                     /note="color: #85dae9; direction: RIGHT"
     misc_RNA        4536..4643
                     /label=RNAI transcript
                     /note="color: #c6c9d1; direction: RIGHT"
     primer_bind     4557..4576
                     /label=1832
                     /note="color: black; sequence: CTGCTGAAGCCAGTTACCTT; added:
                     2024-11-04"
     primer_bind     4574..4595
                     /label=H356
                     /note="color: black; sequence: CTTCGGAAAAAGAGTTGGTAGC; 
                     added: 2024-11-04"
     primer_bind     4590..4619
                     /label=K347
                     /note="color: black; sequence: 
                     ggtagctcttgatccggcaaacaaaccacc; added: 2024-11-04"
     primer_bind     complement(4615..4633)
                     /label=K346
                     /note="color: black; sequence: ccaccgctaccagcggtgg; added: 
                     2024-11-04"
     primer_bind     complement(4646..4669)
                     /label=2031
                     /note="color: black; sequence: TTTCTGCGCGTAATCTGCTGCTTG; 
                     added: 2024-11-04"
     primer_bind     complement(4649..4668)
                     /label=1644
                     /note="color: black; sequence: TTCTGCGCGTAATCTGCTGC; added:
                     2024-11-04"
     primer_bind     4650..4669
                     /label=1216
                     /note="color: black; sequence: CAGCAGATTACGCGCAGAAA; added:
                     2024-11-04"
     primer_bind     complement(4652..4668)
                     /label=1629
                     /note="color: black; sequence: ttctgcgcgtaatctgc; added: 
                     2024-11-04"
     -10_signal      4656..4661
                     /label=RNAII promoter (counter-clockwise)
                     /label=RNAII promoter (counter-clockwise); CGTAAT
                     /note="color: #9eafd2"
     -35_signal      4677..4682
                     /label=RNAII promoter (counter-clockwise)
                     /label=RNAII promoter (counter-clockwise); TTGAGA
                     /note="color: #d6b295"
     primer_bind     4683..4706
                     /label=E868
                     /note="color: black; sequence: gaagatcctttgatcttttctacg; 
                     added: 2024-11-04"
     primer_bind     complement(4715..4738)
                     /label=C133
                     /note="color: black; sequence: CGTGAGTTTTCGTTCCACTGAGCG; 
                     added: 2024-11-04"
     primer_bind     4715..4738
                     /label=C134
                     /note="color: black; sequence: CGCTCAGTGGAACGAAAACTCACG; 
                     added: 2024-11-04"
     primer_bind     4732..4756
                     /label=3485
                     /note="color: black; sequence: actcacgttaagggattttggtcat; 
                     added: 2024-11-04"
     primer_bind     complement(4732..4756)
                     /label=3486
                     /note="color: black; sequence: ATGACCAAAATCCCTTAACGTGAGT; 
                     added: 2024-11-04"
     primer_bind     4768..4788
                     /label=D341
                     /note="color: black; sequence: AAAGGATCTTCACCTAGATCC; 
                     added: 2024-11-04"
     primer_bind     complement(4826..4855)
                     /label=G335
                     /note="color: black; sequence: 
                     taactgtcagaccaagtttactcatatata; added: 2024-11-04"
     primer_bind     4826..4855
                     /label=G336
                     /note="color: black; sequence: 
                     tatatatgagtaaacttggtctgacagtta; added: 2024-11-04"
     primer_bind     complement(4833..4852)
                     /label=H833
                     /note="color: black; sequence: CTGTCAGACCAAGTTTACTC; added:
                     2024-11-04"
     misc_feature    complement(4853..5713)
                     /label=BLA (ampR)
                     /note="color: #a6acb3; direction: LEFT"
     CDS             complement(4856..5515)
                     /codon_start=1
                     /label=AmpR
                     /note="color: #993366"
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHL
                     TDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEA
                     IPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFI
                     ADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     primer_bind     4856..4883
                     /label=C692
                     /note="color: black; sequence: 
                     ccaatgcttaatcagtgaggcacctatc; added: 2024-11-04"
     primer_bind     complement(4856..4883)
                     /label=C693
                     /note="color: black; sequence: 
                     gataggtgcctcactgattaagcattgg; added: 2024-11-04"
     primer_bind     4875..4903
                     /label=C114
                     /note="color: black; sequence: 
                     gcacctatctcagcgatctgtctatttcg; added: 2024-11-04"
     primer_bind     complement(4875..4903)
                     /label=C115
                     /note="color: black; sequence: 
                     cgaaatagacagatcgctgagataggtgc; added: 2024-11-04"
     primer_bind     complement(4883..4910)
                     /label=A541
                     /note="color: black; sequence: 
                     ggatgaacgaaatagacagatcgctgag; added: 2024-11-04"
     primer_bind     4902..4926
                     /label=6923
                     /note="color: black; sequence: CGTTCATCCATAGTTGCCTGACTCC; 
                     added: 2024-11-04"
     primer_bind     complement(4902..4926)
                     /label=6924
                     /note="color: black; sequence: GGAGTCAGGCAACTATGGATGAACG; 
                     added: 2024-11-04"
     primer_bind     complement(4980..5008)
                     /label=K456
                     /note="color: black; sequence: 
                     GGTGAGCGTGGGTCACGCGGTATCATTGC; added: 2024-11-04"
     primer_bind     4980..5008
                     /label=K457
                     /note="color: black; sequence: 
                     GCAATGATACCGCGTGACCCACGCTCACC; added: 2024-11-04"
     primer_bind     5005..5059
                     /label=3050
                     /note="color: black; sequence: 
                     CACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAG; 
                     added: 2024-11-04"
     primer_bind     5062..5121
                     /label=3051
                     /note="color: black; sequence: 
                     GTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGA
                     G; added: 2024-11-04"
     primer_bind     complement(5067..5086)
                     /label=1532
                     /note="color: black; sequence: gaggcggataaagttgcagg; added:
                     2024-11-04"
     primer_bind     5067..5086
                     /label=1533
                     /note="color: black; sequence: cctgcaactttatccgcctc; added:
                     2024-11-04"
     primer_bind     complement(5099..5121)
                     /label=4944
                     /note="color: black; sequence: ctctagcttcccggcaacaatta; 
                     added: 2024-11-04"
     primer_bind     complement(5099..5118)
                     /label=3943
                     /note="color: black; sequence: TAGCTTCCCGGCAACAATTA; added:
                     2024-11-04"
     primer_bind     5101..5124
                     /label=4945
                     /note="color: black; sequence: attgttgccgggaagctagagtaa; 
                     added: 2024-11-04"
     primer_bind     5122..5181
                     /label=3052
                     /note="color: black; sequence: 
                     TAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTG
                     G; added: 2024-11-04"
     primer_bind     complement(5173..5202)
                     /label=6036
                     /note="color: black; sequence: 
                     taccaaacgaCGAGCGTGACACCACGATGC; added: 2024-11-04"
     primer_bind     complement(5173..5202)
                     /label=6042
                     /note="color: black; sequence: 
                     taccaaacgaCGAGCGTGACACCACGATGC; added: 2024-11-04"
     primer_bind     5183..5213
                     /label=6037
                     /note="color: black; sequence: 
                     gtcacgctcgTCGTTTGGTATGGCTTCATTC; added: 2024-11-04"
     primer_bind     5183..5213
                     /label=6043
                     /note="color: black; sequence: 
                     gtcacgctcgTCGTTTGGTATGGCTTCATTC; added: 2024-11-04"
     primer_bind     5188..5206
                     /label=9778
                     /note="color: black; sequence: gctcgtcgtttggtatggc; added: 
                     2024-11-04"
     primer_bind     5191..5208
                     /label=3550
                     /note="color: black; sequence: CGTCGTTTGGTATGGCTT; added: 
                     2024-11-04"
     primer_bind     5194..5219
                     /label=7050
                     /note="color: black; sequence: cgtttggtatggcttcattcagctcc; 
                     added: 2024-11-04"
     primer_bind     complement(5194..5219)
                     /label=7051
                     /note="color: black; sequence: ggagctgaatgaagccataccaaacg; 
                     added: 2024-11-04"
     primer_bind     5219..5252
                     /label=E308
                     /note="color: black; sequence: 
                     CGGTTCCCAACGATCAAGGCGAGTTACATGATCC; added: 2024-11-04"
     primer_bind     complement(5219..5252)
                     /label=E309
                     /note="color: black; sequence: 
                     GGATCATGTAACTCGCCTTGATCGTTGGGAACCG; added: 2024-11-04"
     primer_bind     5294..5316
                     /label=6292
                     /note="color: black; sequence: GATCGTTGTCAGAAGTAAGTTGG; 
                     added: 2024-11-04"
     primer_bind     complement(5294..5316)
                     /label=6293
                     /note="color: black; sequence: CCAACTTACTTCTGACAACGATC; 
                     added: 2024-11-04"
     primer_bind     5357..5378
                     /label=H353
                     /note="color: black; sequence: TTCTCTTACTGTCATGCCATCC; 
                     added: 2024-11-04"
     primer_bind     5472..5494
                     /label=C112
                     /note="color: black; sequence: cgggataataccgcgccacatag; 
                     added: 2024-11-04"
     primer_bind     complement(5472..5494)
                     /label=C113
                     /note="color: black; sequence: ctatgtggcgcggtattatcccg; 
                     added: 2024-11-04"
     primer_bind     5524..5543
                     /label=876
                     /note="color: black; sequence: AACGTTCTTCGGGGCGAAAA; added:
                     2024-11-04"
     primer_bind     complement(5587..5624)
                     /label=C443
                     /note="color: black; sequence: 
                     agtaaaagatgctgaagatcagttgggtgcacgagtgg; added: 2024-11-04"
     primer_bind     5595..5638
                     /label=C444
                     /note="color: black; sequence: 
                     gcacccaactgatcttcagcatcttttactttcaccagcgtttc; added: 
                     2024-11-04"
     primer_bind     complement(5642..5661)
                     /label=3944
                     /note="color: black; sequence: GCCTTCCTGTTTTTGCTCAC; added:
                     2024-11-04"
     sig_peptide     5645..5713
                     /label=bla sig_peptide
                     /note="color: #d59687"
     primer_bind     complement(5692..5740)
                     /label=H892
                     /note="color: black; sequence: 
                     cttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtg; added: 
                     2024-11-04"
     primer_bind     5714..5741
                     /label=H834
                     /note="color: black; sequence: 
                     ACTCTTCCTTTTTCAATATTATTGAAGC; added: 2024-11-04"
     primer_bind     5714..5740
                     /label=H893
                     /note="color: black; sequence: actcttcctttttcaatattattgaag;
                     added: 2024-11-04"
     primer_bind     complement(5740..5769)
                     /label=C691
                     /note="color: black; sequence: 
                     CGCTCATGAGACAATAACCCTGATAAATGC; added: 2024-11-04"
     primer_bind     5740..5769
                     /label=C694
                     /note="color: black; sequence: 
                     GCATTTATCAGGGTTATTGTCTCATGAGCG; added: 2024-11-04"
     primer_bind     5746..5768
                     /label=2866
                     /note="color: black; sequence: ATCAGGGTTATTGTCTCATGAGC; 
                     added: 2024-11-04"
     primer_bind     5747..5768
                     /label=3172
                     /note="color: black; sequence: TCAGGGTTATTGTCTCATGAGC; 
                     added: 2024-11-04"
     primer_bind     complement(5747..5768)
                     /label=3173
                     /note="color: black; sequence: GCTCATGAGACAATAACCCTGA; 
                     added: 2024-11-04"
     primer_bind     5749..5770
                     /label=2920
                     /note="color: black; sequence: AGGGTTATTGTCTCATGAGCGG; 
                     added: 2024-11-04"
     primer_bind     5750..5770
                     /label=4732
                     /note="color: black; sequence: GGGTTATTGTCTCATGAGCGG; 
                     added: 2024-11-04"
     -10_signal      5757..5762
                     /label=bla promoter (counter-clockwise)
                     /label=bla promoter (counter-clockwise); GAGACA
                     /note="color: #d6b295"
     -35_signal      5778..5783
                     /label=bla promoter (counter-clockwise)
                     /label=bla promoter (counter-clockwise); TTCAAA
                     /note="color: #c7b0e3"
     primer_bind     5784..5803
                     /label=4273
                     /note="color: black; sequence: TGTATTTAGAAAAATAAACA; added:
                     2024-11-04"
     primer_bind     complement(5792..5818)
                     /label=5943
                     /note="color: black; sequence: cgcggaacccctatttgtttatttttc;
                     added: 2024-11-04"
     primer_bind     join(5800..5818,1..3)
                     /label=F792
                     /note="color: black; sequence: aacaaataggggttccgcgcac; 
                     added: 2024-11-04"
     primer_bind     join(5809..5818,1..9)
                     /label=3467
                     /note="color: black; sequence: GGGTTCCGCGCACATTTCC; added: 
                     2024-11-04"
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggccctt tcgtctcacg cgtttcggtg atgacggtga
      121 aaacctctga cacatgcagc tcccggagac gagtactata atatatgaat tacattatta
      181 atttaaaaga tactagatgg tgcagcagca gcagcagcag tagtagggcc agtattggcg
      241 tttccccctc ctgacttgaa caaccctttt aacgactttg aaatagatag tgacctcttc
      301 aaagagcttt ctctggaaat acggggctca gaagagtcca cattagatga agccattatg
      361 gattgcgagt ttttatttcg tttattcaaa ttaaggtaac taaaaaactc cttttgatga
      421 cctatacata ggaagatcta tagaaacaaa aagattaata actttcaaat atcagaaaaa
      481 tatagaaaca tgtgataagc tcatagacat ataaaaatat gagattccca tctatcttca
      541 ccgctgtttt gttcgctgct tcttctgctt tggctgctcc agctaacacc accaccgaag
      601 acgaaaccgc tcaaatccca gctgaagctg ttatcgacta ctctgacttg gaaggtgact
      661 tcgacgctgc tgctttgcca ttgtctaact ctaccaacaa cggtttgtct tctaccaaca
      721 ccaccatcgc ttctatcgct gctaaggaag aaggtgttca attggacaag agagaagctg
      781 acgcagagac cgaaagtgaa acgtgatttc atgcgtcatt ttgaacattt tgtaaatctt
      841 atttaataat gtgtgcggca attcacattt aatttatgaa tgttttctta acatcgcggc
      901 aactcaagaa acggcaggtt cggatcttag ctactagaga aagaggagaa atactagatg
      961 cgtaaaggcg aagagctgtt cactggtgtc gtccctattc tggtggaact ggatggtgat
     1021 gtcaacggtc ataagttttc cgtgcgtggc gagggtgaag gtgacgcaac taatggtaaa
     1081 ctgacgctga agttcatctg tactactggt aaactgccgg ttccttggcc gactctggta
     1141 acgacgctga cttatggtgt tcagtgcttt gctcgttatc cggaccatat gaagcagcat
     1201 gacttcttca agtccgccat gccggaaggc tatgtgcagg aacgcacgat ttcctttaag
     1261 gatgacggca cgtacaaaac gcgtgcggaa gtgaaatttg aaggcgatac cctggtaaac
     1321 cgcattgagc tgaaaggcat tgactttaaa gaggacggca atatcctggg ccataagctg
     1381 gaatacaatt ttaacagcca caatgtttac atcaccgccg ataaacaaaa aaatggcatt
     1441 aaagcgaatt ttaaaattcg ccacaacgtg gaggatggca gcgtgcagct ggctgatcac
     1501 taccagcaaa acactccaat cggtgatggt cctgttctgc tgccagacaa tcactatctg
     1561 agcacgcaaa gcgttctgtc taaagatccg aacgagaaac gcgatcatat ggttctgctg
     1621 gagttcgtaa ccgcagcggg catcacgcat ggtatggatg aactgtacaa atgaccaggc
     1681 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1741 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1801 ataggtctcg gttccaagga caatagctcg acgattgaag gtagataccc atacgacgtt
     1861 ccagactacg ctctgcaggc tagtggtggt ggtggttctg gtggtggtgg ttctggtggt
     1921 ggtggttctg ctagcgacgt ccaggaactg acaactatat gcgagcaaat cccctcacca
     1981 actttagaat cgacgccgta ctctttgtca acgactacta ttttggccaa cgggaaggca
     2041 atgcaaggag tttttgaata ttacaaatca gtaacgtttg tcagtaattg cggttctcac
     2101 ccctcaacaa ctagcaaagg cagccccata aacacacagt atgttttttg aatcctaata
     2161 atggccgcaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     2221 aaaaaaaaaa aaaaaaaaaa aaacctgtca ccggatgtgt tttccggtct gatgagtccg
     2281 tgaggacgaa acaggactag gctgcgaatt tcttatgatt tatgattttt attattaaat
     2341 aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa
     2401 aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg
     2461 aggtcgctct taagcaagga ttttcttaac ttcttcggcg acagcatcac cgacttcggt
     2521 ggtactgttg gaaccaccta aatcaccagt tctgatacct gcatccaaaa cctttttaac
     2581 tgcatcttca atggccttac cttcttcagg caagttcaat gacaatttca acatcattgc
     2641 agcagacaag atagtggcga tagggttgac cttattcttt ggcaaatctg gagcagaacc
     2701 gtggcatggt tcgtacaaac caaatgcggt gttcttgtct ggcaaagagg ccaaggacgc
     2761 agatggcaac aaacccaagg aacctgggat aacggaggct tcatcggaga tgatatcacc
     2821 aaacatgttg ctggtgatta taataccatt taggtgggtt gggttcttaa ctaggatcat
     2881 ggcggcagaa tcaatcaatt gatgttgaac cttcaatgta ggaaattcgt tcttgatggt
     2941 ttcctccaca gtttttctcc ataatcttga agaggccaaa acattagctt tatccaagga
     3001 ccaaataggc aatggtggct catgttgtag ggccatgaaa gcggccattc ttgtgattct
     3061 ttgcacttct ggaacggtgt attgttcact atcccaagcg acaccatcac catcgtcctc
     3121 ctttctctta ccaaagtaaa tacctcccac taattctctg acaacaacga agtcagtacc
     3181 tttagcaaat tgtggcttga ttggagataa gtctaaaaga gagtcggatg caaagttaca
     3241 tggtcttaag ttggcgtaca attgaagttc tttacggatt tttagtaaac cttgttcagg
     3301 tctaacacta ccggttcccc atttaggacc acccacagca cctaacaaaa cggcatcagc
     3361 cttcttggag gcttccagcg cctcatctgg aagtggaaca cctgtagcat cgatagcagc
     3421 accaccaatt aaatgatttt cgaaatcgaa cttgacattg gaacgaacat cagaaatagc
     3481 tttaagaacc ttaatggctt cggctgtgat ttcttgacca acgtggtcac ctggcaaaac
     3541 gacgatcttc ttaggggcag acattttata attattataa aaactttcat ataaaaagga
     3601 gttttttagt taccttaatt gaaataaacg aaataaaaac tcgccctcag actcagattg
     3661 gtgtttcaaa gatcctcaag gcaatagaat aacagatttt gatagtatta ataaagaatt
     3721 aggtcttggt agaagagatg taaaattaga taaaggtcat gatgatttaa ttaaattatg
     3781 tactgaaaaa atagatagta ctattaattg cgttgcgctc actgcccgct ttccagtcgg
     3841 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3901 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3961 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4021 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4081 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4141 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4201 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4261 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4321 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4381 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4441 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     4501 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     4561 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4621 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4681 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4741 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     4801 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4861 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4921 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4981 caatgatacc gcgtgaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5041 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5101 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5161 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5221 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5281 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5341 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5401 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     5461 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     5521 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     5581 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5641 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5701 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5761 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcg
//
