LOCUS       pRW404_-_2μ-KanMX-Bxb1- 5764 bp ds-DNA     circular     06-NOV-2024
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pCas_Met15_B.ape
            ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     rep_origin      complement(13..601)
                     /label="ori"
     primer          complement(35..58)
                     /label="H995"
                     /note="sequence: GCGTCGATTTTTGTGATGCTCGTC"
     primer          90..116
                     /label="H996"
                     /note="sequence: TATAAAGATACCAGGCGTTTCCCCCTG"
     misc_feature    631..1506
                     /label="2µ origin"
     protein_bind    complement(1592..1639)
                     /label="FRT"
     primer          complement(1656..1678)
                     /label="RW.2u.97-4.GB.Rvs"
                     /note="sequence: cgtctgcaattatcggccagttcgttgcattttcggaagcgctcg"
     primer          complement(1768..1827)
                     /label="H805 Bacbone1 rv"
                     /note="sequence: aagctaaacagatctctagacctatacataacacatatacgcatttaagcataaacacgc"
     primer          1793..1830
                     /label="H806 bb1 fw"
                     /note="sequence: tgtgttatgtataggtctagagatctgtttagcttgcc"
     misc_feature    1802..1830
                     /label="xx"
     promoter        1858..2201
                     /label="TEF promoter"
     gene            1858..3214
                     /label="kanMX"
     primer          2174..2220
                     /label=6120
                     /note="sequence: ctcacatcacatccgaacataaacaaccATGGGTAAGGAAAAGACTC"
     CDS             2202..3011
                     /label="aminoglycoside phosphotransferase"
     primer          complement(2397..2421)
                     /label="A955"
                     /note="sequence: tcagccagtttagtctgaccatctc"
     primer          2671..2699
                     /label="F848"
                     /note="sequence: gtttggttgatgcgagtgattttgatgac"
     terminator      3017..3214
                     /label="TEF terminator"
     CDS             complement(3412..3828)
                     /label="YJL027C"
     primer          3870..3891
                     /label=7338
                     /note="sequence: ctttttcgctcttcttcttgtc"
     primer          complement(3948..3985)
                     /label=6958
                     /note="sequence: catGGTAATTGGACAAATAAATACGTGTATTAAGTTTG"
     primer          3961..4003
                     /label="RW.Bxb1.pRNR2.GB.Fwd"
                     /note="sequence: cgtatttatttgtccaattaccatggtgagagccctggtagtc"
     misc_feature    3983..3984
                     /label="DVLA"
     misc_feature    3983..3985
                     /label="U1d (concatenated sequence 3)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    3983..3985
                     /label="TAGAGACTAGGTCTCAAATG"
                     /note="Geneious type: motif"
     misc_feature    complement(3983..3985)
                     /label="Ligation"
                     /note="Geneious type: ligation"
     misc_feature    3983..5488
                     /label="Bxb1"
     CDS             3983..5488
                     /label="Bxb1"
                     /translation="MVRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLDVSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLVVSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGYLPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQGREPQGREWSATALKRSMISGAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAELVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTVAMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAGSPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSMNVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS*"
     primer          complement(5467..5491)
                     /label="RW.Bxb1.tAHD1.Rvs"
                     /note="sequence: cataagaaattcgcctcgagttattacgacatcccggtgtgtagc"
     primer          5489..5518
                     /label="A619"
                     /note="sequence: taaCTCGAGGcgaatttcttatgatttatg"
     terminator      5498..5722
                     /label="ScADH1 Terminator"
     primer          5723..5743
                     /label="F924"
                     /note="sequence: AGCAAAAGGCCAGCAAAAGGC"
ORIGIN
        1 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
       61 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      121 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      181 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      241 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      301 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      361 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      421 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
      481 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      541 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      601 agaagatcct ttgatctttt ctacactagt cgaagcatct gtgcttcatt ttgtagaaca
      661 aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga
      721 acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta
      781 aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt
      841 acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt
      901 ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact
      961 ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc
     1021 cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga
     1081 ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa
     1141 ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg
     1201 atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc
     1261 tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca ctctatgaat
     1321 agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat aaaaaatgta
     1381 gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt tatataggga
     1441 tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg aagcggtatt
     1501 cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga aagtgcgtct
     1561 tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta gagaatagga
     1621 acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg
     1681 agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat
     1741 atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta tatgtgttat
     1801 gtataggtct agagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac
     1861 atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt
     1921 gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat
     1981 tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag
     2041 ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat
     2101 ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg
     2161 ctaggataca gttctcacat cacatccgaa cataaacaac catgggtaag gaaaagactc
     2221 acgtttcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     2281 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg
     2341 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     2401 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     2461 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggcaaaaca gcattccagg
     2521 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     2581 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     2641 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     2701 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataagctt ttgccattct
     2761 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     2821 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     2881 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     2941 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     3001 agtttttcta atcagtactg acaataaaaa gattcttgtt ttcaagaact tgtcatttgt
     3061 atagtttttt tatattgtag ttgttctatt ttaatcaaat gttagcgtga tttatatttt
     3121 ttttcgcctc gacatcatct gcccagatgc gaagttaagt gcgcagaaag taatatcatg
     3181 cgtcaatcgt atgtgaatgc tggtcgctat actgctgtcg attcgatact aacgccgcca
     3241 tccagtgtcg aaaacgagct ctcgagaacc cttaatgtcg acagtcgaac aagaagcagg
     3301 caaagtttag agcactgccc ctccgcactc aaaaaagaaa aaactaggag gaaaataaaa
     3361 ttctcaacca cacaaacaca taaacacata caaatacaaa tacaagctta tttacttgac
     3421 atcgcgcgat cttccactat tcagcgccgt ccgccctctc tcgtgttttt tgtttacgcg
     3481 acaactatgc gaaatccgga gcaacgggca accgtttggg gaaagaccac acccacgcgc
     3541 gatcgccatg gcaacgaggt cgcacacgcc ccacacccag acctccctgc gagcgggcat
     3601 gggtacaatg tccccgttgc cacagacacc acttcgtagc acagcgcaga gcgtagcgtg
     3661 ttgttgctgc tgacaaaaga aaatttttct tagcaaagca aaggagggga agcacgggca
     3721 gatagcaccg taccataccc ttggaaactc gaaatgaacg aagcaggaaa tgagagaatg
     3781 agagttttgt aggtatatat agcggtagtg tttgcgcgtt accatcatct tctggatcta
     3841 tctattgttc ttttcctcat cactttcccc tttttcgctc ttcttcttgt cttttatttc
     3901 tttctttttt ttaattgttc cctcgattgg ctatctacca aagaatccaa acttaataca
     3961 cgtatttatt tgtccaatta ccatggtgag agccctggta gtcatccgcc tgtcccgcgt
     4021 caccgatgct acgacttcac cggagcgtca gctggagtct tgccagcagc tctgcgccca
     4081 gcgcggctgg gacgtcgtcg gggtagcgga ggatctggac gtctccgggg cggtcgatcc
     4141 gttcgaccgg aagcgcagac cgaacctggc ccggtggcta gcgttcgagg agcaaccgtt
     4201 tgacgtgatc gtggcgtacc gggtagatcg gttgacccga tcgatccggc atcttcagca
     4261 gctggtccac tgggccgagg accacaagaa gctggtcgtc tccgcgaccg aagcgcactt
     4321 cgatacgacg acgccgtttg cggcggtcgt catcgcgctt atgggaacgg tggcgcagat
     4381 ggaattagaa gcgatcaaag agcggaaccg ttcggctgcg catttcaata tccgcgccgg
     4441 gaaataccga ggatccctgc cgccgtgggg atacctgcct acgcgcgtgg acggggagtg
     4501 gcggctggtg ccggaccctg tgcagcgaga gcgcatcctc gaggtgtatc accgcgtcgt
     4561 cgacaaccac gagccgctgc atctggtggc ccacgacctg aaccggcgtg gtgtcctgtc
     4621 gccgaaggac tacttcgcgc agctgcaagg ccgcgagccg cagggccggg agtggtcggc
     4681 taccgcgctg aagcgatcga tgatctccgg ggcgatgctc gggtacgcga ctctgaacgg
     4741 taagaccgtc cgagacgacg acggagcccc gctggtgcgg gctgagccga tcctgacccg
     4801 tgagcagctg gaggcgctgc gcgccgagct cgtcaagacc tcccgggcga agcccgcggt
     4861 gtctaccccg tcgctgctgc tgcgggtgtt gttctgcgcg gtgtgcgggg agcccgcgta
     4921 caagttcgcc gggggaggac gtaagcaccc gcgctaccgc tgccgctcga tggggttccc
     4981 gaagcactgc gggaacggca cggtggcgat ggccgagtgg gacgcgttct gcgaggagca
     5041 ggtactggat ctgctcgggg acgcggagcg tctggagaaa gtctgggtag cgggctcgga
     5101 ctccgcggtc gaactcgcgg aggtgaacgc ggagctggtg gacctgacgt cgctgatcgg
     5161 ctccccggcc taccgggcgg gctctccgca gcgagaagca ctggatgccc gtattgcggc
     5221 gctggccgcg cggcaagagg agctggaggg cctggaggct cgcccgtctg gctgggagtg
     5281 gcgcgaaacc gggcagcggt tcggggactg gtggcgggag caggacaccg cggcaaagaa
     5341 cacctggctt cggtcgatga acgttcggct gacgttcgac gtccgcggcg ggctgactcg
     5401 cacgatcgac ttcggggatc ttcaggagta cgagcagcat ctcaggctcg gcagcgtggt
     5461 cgaacggcta cacaccggga tgtcgtaata actcgaggcg aatttcttat gatttatgat
     5521 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta
     5581 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc
     5641 tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc cgagcaaatg
     5701 cctgcaaatc gctccccatt tcagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5761 ccgc
//