LOCUS       PB-PE_iSM       13112 bp DNA     circular SYN 06-MAR-2025
DEFINITION  Ligation of ~PmlI-iSM HiFi amp-BamHI~ into ~BamHI-PB-PE_KK-PmlI~.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 6, 2025 from SnapGene Viewer 6.2.2
            https://www.snapgene.com
COMMENT     Vector molecule:  ~BamHI-PB-PE_KK-PmlI~
               Fragment ends: BamHI and PmlI
               Fragment size: 11803
            Insert molecule:  ~PmlI-iSM HiFi amp-BamHI~
               Fragment ends: PmlI and BamHI
               Fragment size: 1363
FEATURES             Location/Qualifiers
     source          1..13112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    294..369
                     /label=pegRNA scaffold
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    410..2130
                     /label=CAG promoter
                     /note="color: #ff99cc"
     misc_feature    2182..2238
                     /label=bpSV40 NLS
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    2239..5532
                     /label=Cas9(N)_H840A
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    2896..2898
                     /label=R221K
                     /note="color: #c0c0c0"
     misc_feature    3415..3417
                     /label=N394K
                     /note="color: #c0c0c0"
     misc_feature    4753..4755
                     /label=H840A
                     /note="color: #c0c0c0"
     misc_feature    5533..6315
                     /label=Smac PID
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    6316..6414
                     /label=33 AA linker
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    6415..8445
                     /label=PE2 M-MLV RT
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    8458..8508
                     /label=bpSV40 NLS
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    8509..8574
                     /label=P2A
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    8575..10173
                     /label=puro_dTK
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    10174..10425
                     /label=bGH p(A)
                     /note="color: #00ccff"
     misc_feature    10434..10675
                     /label=3' piggyBac repeat
                     /note="color: #00ccff"
     misc_feature    10737..11325
                     /label=pUC ori
                     /note="color: #ffff00"
     misc_feature    complement(11496..12356)
                     /label=AmpR
                     /note="color: #cc0000; direction: LEFT"
     misc_feature    12357..12461
                     /label=AmpR promoter
                     /note="color: #ff99cc"
     misc_feature    12746..13059
                     /label=5' piggyBac repeat
                     /note="color: #00ccff"
     misc_feature    join(13060..13112,1..265)
                     /label=U6 promoter
                     /note="color: #ff99cc"
ORIGIN
        1 caaggtcggg caggaagagg gcctatttcc catgattcct tcatatttgc atatacgata
       61 caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca
      121 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      181 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      241 tatcttgtgg aaaggacgaa acaccgggtc ttcgtggagc tcatcgaaga cctgttttag
      301 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
      361 agtcggtgca gagaccgaat cgaccagagg tctcgttttt ttctagacgc tagcgacatt
      421 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      481 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      541 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      601 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      661 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      721 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      781 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
      841 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
      901 cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg
      961 aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
     1021 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct
     1081 gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga
     1141 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     1201 tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg
     1261 ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
     1321 cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc
     1381 gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     1441 ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
     1501 agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag
     1561 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     1621 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     1681 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     1741 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     1801 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     1861 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     1921 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     1981 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2041 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2101 tggttattgt gctgtctcat cattttggca aagaattgat agcggccgct aatacgactc
     2161 actataggga gagccgccac catgaaacgg acagccgacg gaagcgagtt cgagtcacca
     2221 aagaagaagc ggaaagtcga caagaagtac agcatcggcc tggacatcgg caccaactct
     2281 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg
     2341 ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc
     2401 ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg
     2461 aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac
     2521 agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg
     2581 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc
     2641 taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat
     2701 ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac
     2761 cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg
     2821 ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga
     2881 ctgagcaaga gcagaaagct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat
     2941 ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac
     3001 ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg
     3061 gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac
     3121 ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc
     3181 cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg
     3241 aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc
     3301 aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc
     3361 atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaagaga
     3421 gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac
     3481 ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac
     3541 aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg
     3601 gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc
     3661 tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg
     3721 accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac
     3781 gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga
     3841 aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc
     3901 aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc
     3961 gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac
     4021 gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt
     4081 ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg
     4141 ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga
     4201 tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc
     4261 ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag
     4321 ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc
     4381 cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag
     4441 ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag
     4501 cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag
     4561 aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc
     4621 ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac
     4681 ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac
     4741 tacgatgtgg acgctatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag
     4801 gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc
     4861 gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga
     4921 aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc
     4981 ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg
     5041 gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg
     5101 atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg
     5161 cgcgagatca acaactacca ccacgcccac gacgcctacc taaacgccgt cgtgggaacc
     5221 gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg
     5281 tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag
     5341 tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc
     5401 gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat
     5461 aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg
     5521 aaaaagaccg agatccagac cgtgggacag aatggaggcc tgttcgacga caaccccaag
     5581 agccccctgg aggtgacccc cagcaaactg gtgcccctga agaaagagct gaaccccaag
     5641 aaatacggcg gctaccagaa gcccaccacc gcctaccccg tgctgctgat caccgacacc
     5701 aagcagctga tccccatcag cgtgatgaac aagaaacagt tcgagcagaa ccccgtgaag
     5761 ttcctgcggg accggggcta ccagcaggtg ggcaagaacg acttcatcaa gctgcccaag
     5821 tacaccctgg tggacatcgg cgacggcatc aagcggctgt gggccagcag caaggagatc
     5881 cacaagggca accagctggt ggtgagcaag aagagccaga tcctgctgta ccacgcccac
     5941 cacctggaca gcgacctgag caacgactac ctgcagaacc acaaccagca gttcgacgtg
     6001 ctgttcaacg agatcatcag cttcagcaaa aagtgcaagc tgggcaagga gcacatccag
     6061 aagatcgaga acgtgtacag caacaagaag aacagcgcca gcatcgagga gctggccgag
     6121 agctttatca aactgctcgg cttcacccag ctgggcgcca ccagcccctt caacttcctg
     6181 ggcgtgaagc tgaaccagaa gcagtacaag ggcaagaagg actacatcct gccctgcacc
     6241 gagggcaccc tgatcagaca gagcatcacc ggactgtacg aaaccagagt ggatctgagc
     6301 aagatcggcg aggattctgg aggatctagc ggaggatcct ctggcagcga gacaccagga
     6361 acaagcgagt cagcaacacc agagagcagt ggcggcagca gcggcggcag cagcacccta
     6421 aatatagaag atgagtatcg gctacatgaa acctcaaaag agccagatgt ttctctaggg
     6481 tccacatggc tgtctgattt tcctcaggcc tgggcggaaa ccgggggcat gggactggca
     6541 gttcgccaag ctcctctgat catacctctg aaagcaacct ctacccccgt gtccataaaa
     6601 caatacccca tgtcacaaga agccagactg gggatcaagc cccacataca gagactgttg
     6661 gaccagggaa tactggtacc ctgccagtcc ccctggaaca cgcccctgct acccgttaag
     6721 aaaccaggga ctaatgatta taggcctgtc caggatctga gagaagtcaa caagcgggtg
     6781 gaggacatcc accccaccgt gcccaaccct tacaacctct tgagcgggct cccaccgtcc
     6841 caccagtggt acactgtgct tgatttaaag gatgcctttt tctgcctgag actccacccc
     6901 accagtcagc ctctcttcgc ctttgagtgg agagatccag agatgggaat ctcaggacaa
     6961 ttgacctgga ccagactccc acagggtttc aaaaacagtc ccaccctgtt taatgaggca
     7021 ctgcacaggg acctagcaga cttcagaatc cagcacccag acttgatcct gctacagtac
     7081 gtggatgact tactgctggc cgccacttct gagctagact gccaacaagg tactcgggcc
     7141 ctgttacaaa ccctagggaa cctcgggtat cgggcctcgg ccaagaaagc ccaaatttgc
     7201 cagaaacagg tcaagtatct ggggtatctt ctaaaagagg gtcagagatg gctgactgag
     7261 gccagaaaag agactgtgat ggggcagcct actcccaaga cccctcgaca actaagggag
     7321 ttcctaggga aggcaggctt ctgtcgcctc ttcatccctg ggtttgcaga aatggcagcc
     7381 cccctgtacc ctctcaccaa accggggact ctgtttaatt ggggcccaga ccaacaaaag
     7441 gcctatcaag aaatcaagca agctcttcta actgccccag ccctggggtt gccagatttg
     7501 actaagccct ttgaactctt tgtcgacgag aagcagggct acgccaaagg tgtcctaacg
     7561 caaaaactgg gaccttggcg tcggccggtg gcctacctgt ccaaaaagct agacccagta
     7621 gcagctgggt ggcccccttg cctacggatg gtagcagcca ttgccgtact gacaaaggat
     7681 gcaggcaagc taaccatggg acagccacta gtcattctgg ccccccatgc agtagaggca
     7741 ctagtcaaac aaccccccga ccgctggctt tccaacgccc ggatgactca ctatcaggcc
     7801 ttgcttttgg acacggaccg ggtccagttc ggcccggtgg tagccctgaa cccggctacg
     7861 ctgctcccac tgcctgagga agggctgcaa cacaactgcc ttgatatcct ggccgaagcc
     7921 cacggaaccc gacccgacct aacggaccag ccgctcccag acgccgacca cacctggtac
     7981 acggatggaa gcagtctctt acaagaggga cagcgtaagg cgggagctgc ggtgaccacc
     8041 gagacagagg taatctgggc taaagccctg ccagccggga catccgctca gcgggctgaa
     8101 ctgatagcac tcacccaggc cctaaagatg gcagaaggta agaagctaaa tgtttatact
     8161 gatagccgtt atgcttttgc tactgcccat atccatggag aaatatacag aaggcgtggg
     8221 tggctcacat cagaaggcaa agagatcaaa aataaagacg agatcttggc cctactaaaa
     8281 gccctctttc tgcccaaaag acttagcata atccattgtc caggacatca aaagggacac
     8341 agcgccgagg ctagaggcaa ccggatggct gaccaagcgg cccgaaaggc agccatcaca
     8401 gagactccag acacctctac cctcctcata gaaaattcat caccctctgg cggctcaaaa
     8461 agaaccgccg acggcagcga attcgagccc aagaagaaga ggaaagtcgg ctccggcgct
     8521 accaatttct ccctgctcaa gcaagccgga gacgtcgagg aaaacccagg cccaatgacc
     8581 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     8641 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     8701 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     8761 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     8821 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     8881 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     8941 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     9001 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagaca
     9061 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     9121 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc cggctccatg
     9181 cccacgctac tgcgggttta tatagacggt cctcacggga tggggaaaac caccaccacg
     9241 caactgctgg tggccctggg ttcgcgcgac gatatcgtct acgtacccga gccgatgact
     9301 tactggcagg tgctgggggc ttccgagaca atcgcgaaca tctacaccac acaacaccgc
     9361 ctcgaccagg gtgagatatc ggccggggac gcggcggtgg taatgacaag cgcccagata
     9421 acaatgggca tgccttatgc cgtgaccgac gccgttctgg ctcctcatat cgggggggag
     9481 gctgggagct cacatgcccc gcccccggcc ctcaccctca tcttcgaccg ccatcccatc
     9541 gccgccctcc tgtgctaccc ggccgcgcga taccttatgg gcagcatgac cccccaggcc
     9601 gtgctggcgt tcgtggccct catcccgccg accttgcccg gcacaaacat cgtgttgggg
     9661 gcccttccgg aggacagaca catcgaccgc ctggccaaac gccagcgccc cggcgagcgg
     9721 cttgacctgg ctatgctggc cgcgattcgc cgcgtttacg ggctgcttgc caatacggtg
     9781 cggtatctgc agggcggcgg gtcgtggcgg gaggattggg gacagctttc ggggacggcc
     9841 gtgccgcccc agggtgccga gccccagagc aacgcgggcc cacgacccca tatcggggac
     9901 acgttattta ccctgtttcg ggcccccgag ttgctggccc ccaacggcga cctgtacaac
     9961 gtgtttgcct gggccttgga cgtcttggcc aaacgcctcc gtcccatgca cgtctttatc
    10021 ctggattacg accaatcgcc cgccggctgc cgggacgccc tgctgcaact tacctccggg
    10081 atggtccaga cccacgtcac cacccccggc tccataccga cgatctgcga cctggcgcgc
    10141 acgtttgccc gggagatggg ggaggctaac tgagctctag agctcgctga tcagcctcga
    10201 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    10261 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    10321 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    10381 gggaggacaa tagcaggcat gctggggatg cggtgggctc tatggggcgc gcctaaaagt
    10441 tttgttactt tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat
    10501 aaataaattg tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat
    10561 atctattcaa attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt
    10621 ttacgcatga ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaaacatg
    10681 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10741 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10801 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10861 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10921 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10981 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    11041 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    11101 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    11161 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    11221 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    11281 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    11341 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    11401 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    11461 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    11521 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    11581 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgactc
    11641 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    11701 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11761 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    11821 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11881 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11941 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    12001 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    12061 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    12121 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    12181 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    12241 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    12301 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    12361 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    12421 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    12481 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    12541 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
    12601 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
    12661 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
    12721 attgtactga gagtgcacca atttgttaac cctagaaaga tagtctgcgt aaaattgacg
    12781 catgcattct tgaaatattg ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt
    12841 taggacatct cagtcgccgc ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg
    12901 tcactgattt tgaactataa cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg
    12961 tgacttttaa gatttaactc atacgataat tatattgtta tttcatgttc tacttacgtg
    13021 ataacttatt atatatatat tttcttgtta tagatatcat gtacaaaaaa gcaggcttta
    13081 aaggaaccaa ttcagtcgac tggacccggt ac
//
