LOCUS       PB-PE_iSM_PPL2       13145 bp DNA     circular SYN 06-MAR-2025
DEFINITION  Ligation of ~PmlI-iSM HiFi amp-BamHI~ into ~BamHI-PB-PE_KK-PmlI~.
ACCESSION   .
VERSION     .
KEYWORDS    PB-PE iSM_PPL2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 6, 2025 from SnapGene Viewer 6.2.2
            https://www.snapgene.com
COMMENT     Vector molecule:  ~BamHI-PB-PE_KK-PmlI~
               Fragment ends: BamHI and PmlI
               Fragment size: 11803
            Insert molecule:  ~PmlI-iSM HiFi amp-BamHI~
               Fragment ends: PmlI and BamHI
               Fragment size: 1363
FEATURES             Location/Qualifiers
     source          1..13145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    294..369
                     /label=pegRNA scaffold
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    410..2130
                     /label=CAG promoter
                     /note="color: #ff99cc"
     misc_feature    2182..2238
                     /label=bpSV40 NLS
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    2239..5532
                     /label=Cas9(N)_H840A
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    2896..2898
                     /label=R221K
                     /note="color: #c0c0c0"
     misc_feature    3415..3417
                     /label=N394K
                     /note="color: #c0c0c0"
     misc_feature    4753..4755
                     /label=H840A
                     /note="color: #c0c0c0"
     misc_feature    5533..6348
                     /label=Smac PID_PPL2
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    5686..5733
                     /label=PPL2
                     /note="color: #ff6600; direction: RIGHT"
     misc_feature    6349..6447
                     /label=33 AA linker
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    6448..8478
                     /label=PE2 M-MLV RT
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    8491..8541
                     /label=bpSV40 NLS
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    8542..8607
                     /label=P2A
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    8608..10206
                     /label=puro_dTK
                     /note="color: #cc0000; direction: RIGHT"
     misc_feature    10207..10458
                     /label=bGH p(A)
                     /note="color: #00ccff"
     misc_feature    10467..10708
                     /label=3' piggyBac repeat
                     /note="color: #00ccff"
     misc_feature    10770..11358
                     /label=pUC ori
                     /note="color: #ffff00"
     misc_feature    complement(11529..12389)
                     /label=AmpR
                     /note="color: #cc0000; direction: LEFT"
     misc_feature    12390..12494
                     /label=AmpR promoter
                     /note="color: #ff99cc"
     misc_feature    12779..13092
                     /label=5' piggyBac repeat
                     /note="color: #00ccff"
     misc_feature    join(13093..13145,1..265)
                     /label=U6 promoter
                     /note="color: #ff99cc"
ORIGIN
        1 caaggtcggg caggaagagg gcctatttcc catgattcct tcatatttgc atatacgata
       61 caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca
      121 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
      181 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
      241 tatcttgtgg aaaggacgaa acaccgggtc ttcgtggagc tcatcgaaga cctgttttag
      301 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
      361 agtcggtgca gagaccgaat cgaccagagg tctcgttttt ttctagacgc tagcgacatt
      421 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      481 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      541 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      601 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      661 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      721 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      781 tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc
      841 cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg
      901 cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg
      961 aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
     1021 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct
     1081 gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga
     1141 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     1201 tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg
     1261 ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg
     1321 cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc
     1381 gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     1441 ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
     1501 agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag
     1561 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     1621 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     1681 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     1741 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     1801 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     1861 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     1921 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     1981 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2041 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2101 tggttattgt gctgtctcat cattttggca aagaattgat agcggccgct aatacgactc
     2161 actataggga gagccgccac catgaaacgg acagccgacg gaagcgagtt cgagtcacca
     2221 aagaagaagc ggaaagtcga caagaagtac agcatcggcc tggacatcgg caccaactct
     2281 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg
     2341 ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc
     2401 ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg
     2461 aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac
     2521 agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg
     2581 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc
     2641 taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat
     2701 ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac
     2761 cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg
     2821 ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga
     2881 ctgagcaaga gcagaaagct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat
     2941 ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac
     3001 ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg
     3061 gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac
     3121 ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc
     3181 cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg
     3241 aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc
     3301 aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc
     3361 atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaagaga
     3421 gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac
     3481 ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac
     3541 aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg
     3601 gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc
     3661 tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg
     3721 accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac
     3781 gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga
     3841 aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc
     3901 aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc
     3961 gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac
     4021 gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt
     4081 ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg
     4141 ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga
     4201 tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc
     4261 ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag
     4321 ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc
     4381 cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag
     4441 ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag
     4501 cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag
     4561 aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc
     4621 ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac
     4681 ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac
     4741 tacgatgtgg acgctatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag
     4801 gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc
     4861 gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga
     4921 aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc
     4981 ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg
     5041 gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg
     5101 atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg
     5161 cgcgagatca acaactacca ccacgcccac gacgcctacc taaacgccgt cgtgggaacc
     5221 gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg
     5281 tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag
     5341 tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc
     5401 gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat
     5461 aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg
     5521 aaaaagaccg agatccagac cgtgggacag aatggaggcc tgttcgacga caaccccaag
     5581 agccccctgg aggtgacccc cagcaaactg gtgcccctga agaaagagct gaaccccaag
     5641 aaatacggcg gctaccagaa gcccaccacc gcctaccccg tgctggtggt ggccaaagtg
     5701 gaaaagggca agtccaagaa actgaagagt gtgaagcagc tgatccccat cagcgtgatg
     5761 aacaagaaac agttcgagca gaaccccgtg aagttcctgc gggaccgggg ctaccagcag
     5821 gtgggcaaga acgacttcat caagctgccc aagtacaccc tggtggacat cggcgacggc
     5881 atcaagcggc tgtgggccag cagcaaggag atccacaagg gcaaccagct ggtggtgagc
     5941 aagaagagcc agatcctgct gtaccacgcc caccacctgg acagcgacct gagcaacgac
     6001 tacctgcaga accacaacca gcagttcgac gtgctgttca acgagatcat cagcttcagc
     6061 aaaaagtgca agctgggcaa ggagcacatc cagaagatcg agaacgtgta cagcaacaag
     6121 aagaacagcg ccagcatcga ggagctggcc gagagcttta tcaaactgct cggcttcacc
     6181 cagctgggcg ccaccagccc cttcaacttc ctgggcgtga agctgaacca gaagcagtac
     6241 aagggcaaga aggactacat cctgccctgc accgagggca ccctgatcag acagagcatc
     6301 accggactgt acgaaaccag agtggatctg agcaagatcg gcgaggattc tggaggatct
     6361 agcggaggat cctctggcag cgagacacca ggaacaagcg agtcagcaac accagagagc
     6421 agtggcggca gcagcggcgg cagcagcacc ctaaatatag aagatgagta tcggctacat
     6481 gaaacctcaa aagagccaga tgtttctcta gggtccacat ggctgtctga ttttcctcag
     6541 gcctgggcgg aaaccggggg catgggactg gcagttcgcc aagctcctct gatcatacct
     6601 ctgaaagcaa cctctacccc cgtgtccata aaacaatacc ccatgtcaca agaagccaga
     6661 ctggggatca agccccacat acagagactg ttggaccagg gaatactggt accctgccag
     6721 tccccctgga acacgcccct gctacccgtt aagaaaccag ggactaatga ttataggcct
     6781 gtccaggatc tgagagaagt caacaagcgg gtggaggaca tccaccccac cgtgcccaac
     6841 ccttacaacc tcttgagcgg gctcccaccg tcccaccagt ggtacactgt gcttgattta
     6901 aaggatgcct ttttctgcct gagactccac cccaccagtc agcctctctt cgcctttgag
     6961 tggagagatc cagagatggg aatctcagga caattgacct ggaccagact cccacagggt
     7021 ttcaaaaaca gtcccaccct gtttaatgag gcactgcaca gggacctagc agacttcaga
     7081 atccagcacc cagacttgat cctgctacag tacgtggatg acttactgct ggccgccact
     7141 tctgagctag actgccaaca aggtactcgg gccctgttac aaaccctagg gaacctcggg
     7201 tatcgggcct cggccaagaa agcccaaatt tgccagaaac aggtcaagta tctggggtat
     7261 cttctaaaag agggtcagag atggctgact gaggccagaa aagagactgt gatggggcag
     7321 cctactccca agacccctcg acaactaagg gagttcctag ggaaggcagg cttctgtcgc
     7381 ctcttcatcc ctgggtttgc agaaatggca gcccccctgt accctctcac caaaccgggg
     7441 actctgttta attggggccc agaccaacaa aaggcctatc aagaaatcaa gcaagctctt
     7501 ctaactgccc cagccctggg gttgccagat ttgactaagc cctttgaact ctttgtcgac
     7561 gagaagcagg gctacgccaa aggtgtccta acgcaaaaac tgggaccttg gcgtcggccg
     7621 gtggcctacc tgtccaaaaa gctagaccca gtagcagctg ggtggccccc ttgcctacgg
     7681 atggtagcag ccattgccgt actgacaaag gatgcaggca agctaaccat gggacagcca
     7741 ctagtcattc tggcccccca tgcagtagag gcactagtca aacaaccccc cgaccgctgg
     7801 ctttccaacg cccggatgac tcactatcag gccttgcttt tggacacgga ccgggtccag
     7861 ttcggcccgg tggtagccct gaacccggct acgctgctcc cactgcctga ggaagggctg
     7921 caacacaact gccttgatat cctggccgaa gcccacggaa cccgacccga cctaacggac
     7981 cagccgctcc cagacgccga ccacacctgg tacacggatg gaagcagtct cttacaagag
     8041 ggacagcgta aggcgggagc tgcggtgacc accgagacag aggtaatctg ggctaaagcc
     8101 ctgccagccg ggacatccgc tcagcgggct gaactgatag cactcaccca ggccctaaag
     8161 atggcagaag gtaagaagct aaatgtttat actgatagcc gttatgcttt tgctactgcc
     8221 catatccatg gagaaatata cagaaggcgt gggtggctca catcagaagg caaagagatc
     8281 aaaaataaag acgagatctt ggccctacta aaagccctct ttctgcccaa aagacttagc
     8341 ataatccatt gtccaggaca tcaaaaggga cacagcgccg aggctagagg caaccggatg
     8401 gctgaccaag cggcccgaaa ggcagccatc acagagactc cagacacctc taccctcctc
     8461 atagaaaatt catcaccctc tggcggctca aaaagaaccg ccgacggcag cgaattcgag
     8521 cccaagaaga agaggaaagt cggctccggc gctaccaatt tctccctgct caagcaagcc
     8581 ggagacgtcg aggaaaaccc aggcccaatg accgagtaca agcccacggt gcgcctcgcc
     8641 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     8701 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     8761 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     8821 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     8881 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     8941 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     9001 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     9061 gagcgcgccg gggtgcccgc cttcctggag acatccgcgc cccgcaacct ccccttctac
     9121 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     9181 tgcatgaccc gcaagcccgg tgccggctcc atgcccacgc tactgcgggt ttatatagac
     9241 ggtcctcacg ggatggggaa aaccaccacc acgcaactgc tggtggccct gggttcgcgc
     9301 gacgatatcg tctacgtacc cgagccgatg acttactggc aggtgctggg ggcttccgag
     9361 acaatcgcga acatctacac cacacaacac cgcctcgacc agggtgagat atcggccggg
     9421 gacgcggcgg tggtaatgac aagcgcccag ataacaatgg gcatgcctta tgccgtgacc
     9481 gacgccgttc tggctcctca tatcgggggg gaggctggga gctcacatgc cccgcccccg
     9541 gccctcaccc tcatcttcga ccgccatccc atcgccgccc tcctgtgcta cccggccgcg
     9601 cgatacctta tgggcagcat gaccccccag gccgtgctgg cgttcgtggc cctcatcccg
     9661 ccgaccttgc ccggcacaaa catcgtgttg ggggcccttc cggaggacag acacatcgac
     9721 cgcctggcca aacgccagcg ccccggcgag cggcttgacc tggctatgct ggccgcgatt
     9781 cgccgcgttt acgggctgct tgccaatacg gtgcggtatc tgcagggcgg cgggtcgtgg
     9841 cgggaggatt ggggacagct ttcggggacg gccgtgccgc cccagggtgc cgagccccag
     9901 agcaacgcgg gcccacgacc ccatatcggg gacacgttat ttaccctgtt tcgggccccc
     9961 gagttgctgg cccccaacgg cgacctgtac aacgtgtttg cctgggcctt ggacgtcttg
    10021 gccaaacgcc tccgtcccat gcacgtcttt atcctggatt acgaccaatc gcccgccggc
    10081 tgccgggacg ccctgctgca acttacctcc gggatggtcc agacccacgt caccaccccc
    10141 ggctccatac cgacgatctg cgacctggcg cgcacgtttg cccgggagat gggggaggct
    10201 aactgagctc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
    10261 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
    10321 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
    10381 gtggggtggg gcaggacagc aagggggagg attgggagga caatagcagg catgctgggg
    10441 atgcggtggg ctctatgggg cgcgcctaaa agttttgtta ctttatagaa gaaattttga
    10501 gtttttgttt ttttttaata aataaataaa cataaataaa ttgtttgttg aatttattat
    10561 tagtatgtaa gtgtaaatat aataaaactt aatatctatt caaattaata aataaacctc
    10621 gatatacaga ccgataaaac acatgcgtca attttacgca tgattatctt taacgtacgt
    10681 cacaatatga ttatctttct agggttaaac atgtgagcaa aaggccagca aaaggccagg
    10741 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    10801 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    10861 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    10921 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    10981 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    11041 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    11101 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    11161 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    11221 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    11281 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    11341 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    11401 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    11461 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    11521 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    11581 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    11641 tctggcccca gtgctgcaat gataccgcga ctcccacgct caccggctcc agatttatca
    11701 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    11761 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    11821 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    11881 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    11941 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    12001 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    12061 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    12121 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    12181 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    12241 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    12301 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    12361 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    12421 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    12481 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
    12541 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    12601 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    12661 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    12721 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccaatttgtt
    12781 aaccctagaa agatagtctg cgtaaaattg acgcatgcat tcttgaaata ttgctctctc
    12841 tttctaaata gcgcgaatcc gtcgctgtgc atttaggaca tctcagtcgc cgcttggagc
    12901 tcccgtgagg cgtgcttgtc aatgcggtaa gtgtcactga ttttgaacta taacgaccgc
    12961 gtgagtcaaa atgacgcatg attatctttt acgtgacttt taagatttaa ctcatacgat
    13021 aattatattg ttatttcatg ttctacttac gtgataactt attatatata tattttcttg
    13081 ttatagatat catgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggaccc
    13141 ggtac
//
