LOCUS       Lyso-InPGrp             8580 bp    DNA     circular SYN 08-MAR-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 8, 2025 from SnapGene 8.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             895..2160
                     /codon_start=1
                     /label=LAMP1
                     /note="note=""direct strand 
                     translation""translation=""KLAAATMAAPGSARRPLLLLLLLLLLGLMHCA
                     SAAMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENT
                     SDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITD
                     IRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPP
                     APPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNK
                     TSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAA
                     NGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDEN
                     SMLIPIAVGGALAGLVLIVLIAYLVGRKRSHAGYQTIGGGGG"""
                     /note="color: #800040"
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMAN
                     FSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNF
                     TRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHM
                     NNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKY
                     NVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSE
                     GTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNA
                     EEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIV
                     LIAYLVGRKRSHAGYQTIGGGGG"
     CDS             2170..2886
                     /codon_start=1
                     /label=Cerulean
                     /note="color: #66ccff"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2896..3261
                     /codon_start=1
                     /label=PH-Grp1
                     /label=GRP PH
                     /note="color: #005493"
                     /translation="TFFNPDREGWLLKLGGRVKTWKRRWFILTDNCLYYFEYTTDKEPR
                     GIIPLENLSIREVEDPRKPNCFELYNPSHKGQVIKACKTEADGRVVEGNHVVYRISAPS
                     PEEKEEWMKSIKASISRD"
     CDS             3277..3318
                     /codon_start=1
                     /label=PH-pseudoligand
                     /note="color: #d6d6d6"
                     /translation="VAEEEDDEEEDEDD"
     CDS             3334..4068
                     /codon_start=1
                     /label=cpVenus(E172)
                     /note="color: #ffff0a"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVSG
                     EGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYITADKQKNGIKANFKIRHNIE"
     promoter        complement(4132..4150)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     polyA_signal    4176..4400
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      4446..4874
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        4888..5218
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5069..5204
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             5285..6079
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /note="color: #ccffcc"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6253..6374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6423..6439)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    6447..6463
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(join(6471..6477,6478..6495,6496..6501))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6471 .. 6477 / #ffffff / -10
                        2: 6478 .. 6495 / #ffffff
                        3: 6496 .. 6501 / #ffffff / -35"
     protein_bind    6516..6537
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(6825..7413)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(join(7584..8375,8376..8444))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7584 .. 8375 / #ccffcc
                        2: 8376 .. 8444 / #ccffcc / signal sequence
                     Cleavage site after base 8375"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8445..8549)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttatggcg
      901 gcccccggca gcgcccggcg acccctgctg ctgctactgc tgttgctgct gctcggcctc
      961 atgcattgtg cgtcagcagc aatgtttatg gtgaaaaatg gcaacgggac cgcgtgcata
     1021 atggccaact tctctgctgc cttctcagtg aactacgaca ccaagagtgg ccctaagaac
     1081 atgacctttg acctgccatc agatgccaca gtggtgctca accgcagctc ctgtggaaaa
     1141 gagaacactt ctgaccccag tctcgtgatt gcttttggaa gaggacatac actcactctc
     1201 aatttcacga gaaatgcaac acgttacagc gtccagctca tgagttttgt ttataacttg
     1261 tcagacacac accttttccc caatgcgagc tccaaagaaa tcaagactgt ggaatctata
     1321 actgacatca gggcagatat agataaaaaa tacagatgtg ttagtggcac ccaggtccac
     1381 atgaacaacg tgaccgtaac gctccatgat gccaccatcc aggcgtacct ttccaacagc
     1441 agcttcagca ggggagagac acgctgtgaa caagacaggc cttccccaac cacagcgccc
     1501 cctgcgccac ccagcccctc gccctcaccc gtgcccaaga gcccctctgt ggacaagtac
     1561 aacgtgagcg gcaccaacgg gacctgcctg ctggccagca tggggctgca gctgaacctc
     1621 acctatgaga ggaaggacaa cacgacggtg acaaggcttc tcaacatcaa ccccaacaag
     1681 acctcggcca gcgggagctg cggcgcccac ctggtgactc tggagctgca cagcgagggc
     1741 accaccgtcc tgctcttcca gttcgggatg aatgcaagtt ctagccggtt tttcctacaa
     1801 ggaatccagt tgaatacaat tcttcctgac gccagagacc ctgcctttaa agctgccaac
     1861 ggctccctgc gagcgctgca ggccacagtc ggcaattcct acaagtgcaa cgcggaggag
     1921 cacgtccgtg tcacgaaggc gttttcagtc aatatattca aagtgtgggt ccaggctttc
     1981 aaggtggaag gtggccagtt tggctctgtg gaggagtgtc tgctggacga gaacagcatg
     2041 ctgatcccca tcgctgtggg tggtgccctg gcggggctgg tcctcatcgt cctcatcgcc
     2101 tacctcgtcg gcaggaagag gagtcacgca ggctaccaga ctatcggtgg tggcggtggt
     2161 gcggatccca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2221 gagctggacg gcgacgtaaa cggccacagg ttcagcgtgt ccggcgaggg cgagggcgat
     2281 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2341 tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcgcccg ctaccccgac
     2401 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgt
     2461 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2521 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2581 ctggggcaca agctggagta caacgccatc agtgacaacg tctatatcac cgccgacaag
     2641 cagaagaacg gcatcaaggc ccacttcaag atccgccaca acatcgagga cggcagcgtg
     2701 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2761 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2821 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2881 tacaagcgca tgcatacctt cttcaatcca gaccgagaag gctggctgct gaagctgggg
     2941 ggccgtgtga agacctggaa gcggcgctgg ttcatcctca cagacaactg cctctactac
     3001 tttgagtaca ccacggacaa ggagcccagg gggatcatcc ccctggagaa cctgagcatc
     3061 agggaggtgg aggaccctcg gaagccgaac tgctttgagc tttataaccc cagtcacaaa
     3121 gggcaagtca ttaaagcctg caagacggag gcagatggcc gtgtggtgga ggggaaccat
     3181 gttgtatacc ggatctctgc ccccagccct gaggagaagg aggagtggat gaagtccatc
     3241 aaagcgagca tcagtaggga ctctgcaggc ggtagcgtgg ctgaggaaga ggatgacgag
     3301 gaggaagacg aggacgatgg cggcagcgag ctcatgggcg gcgtgcagct cgccgaccac
     3361 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3421 agctaccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3481 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggaggtacc
     3541 ggtggatcta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     3601 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     3661 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
     3721 tggcccaccc tcgtgaccac cctgggctac ggccttcagt gcttcgcccg ctaccccgac
     3781 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     3841 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     3901 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     3961 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
     4021 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagta agaattctgc
     4081 agatatccat cacactggcg gccgctcgag catgcatcta gagggcccta ttctatagtg
     4141 tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     4201 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4261 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     4321 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     4381 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     4441 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4501 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4561 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg
     4621 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4681 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4741 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4801 tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4861 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4921 tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4981 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5041 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     5101 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     5161 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     5221 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt
     5281 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     5341 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     5401 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     5461 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     5521 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     5581 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     5641 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     5701 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     5761 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     5821 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     5881 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     5941 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     6001 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     6061 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     6121 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     6181 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     6241 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6301 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6361 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     6421 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6481 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6541 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6601 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6661 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6721 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6781 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6841 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6901 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6961 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     7021 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7081 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7141 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7201 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     7261 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     7321 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     7381 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     7441 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7501 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7561 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7621 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7681 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7741 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7801 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7861 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7921 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7981 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8041 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8101 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8161 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     8221 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     8281 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     8341 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     8401 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     8461 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8521 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
