LOCUS       Exported                9347 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  1_1.
ACCESSION   1_1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..9347
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             5443..5694
                     /label=GVQW1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=73.8"
                     /note="/match_length=43.1"
                     /note="/other=CDS"
     CDS             complement(6694..8346)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(8393..8892)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(8949..9232)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgacttac aaagtaaaat cattctttaa
     4681 ttattaaaaa aaaatcactg ttgggctttt ttttttaaag gtccataaat tttcaatgca
     4741 atgttgaact ggggaaatgg ttatagcagc tctcacataa gttatttgaa ttaataaaca
     4801 ttatcacagt attaagaagg caaggtaaac aaatagctta agtattttat ttgtttgcaa
     4861 ctaagcttgt taacactata taatgaaaat gtacaaagaa taatgttgca gtttttggtt
     4921 agatttaagt caaagacatt cattaataag gaatcttcga atgtgaatat tccagaaatt
     4981 tctctaagcc atctgatcac aacaactgtc tctgaatatt accttacttt ggaaggtatt
     5041 ggcatacatt tgaattaaac tcagaatttt tcaaaataag ccctaattaa aaaaactatt
     5101 ctatattcaa agtaaaacaa tattaataga actttccttt ccagaaacaa tgaaggaaac
     5161 aaggcatttt gtaaaatgtg gtcctgaaca agtcacagta aaaaataaat gtatacttaa
     5221 cttccacctc ctcaaaacag aggctacttt ctgttcctca gccttggaaa caaggtgaag
     5281 aacagcctat atgcgacttc gggcaggggg ttgccaaaac aaagctgaag cagttgacca
     5341 tggacacctc aaaagaattt gggagaaccc gaccaaagga tccaaattct atgttttttt
     5401 ttttttgaga cgaagtctcg ttttgttgcc caggctggag tgcagtggca tgatctgtgc
     5461 tcactgcaac cgccacctcc agggttcaag tgattctcct gcctcagcct cccaagcagc
     5521 tgggattaca gacgcctgcc accacgcccg gctaactttg ttgtattttt agtagagatg
     5581 gggtttcacc atattggcca gggtggtctc gaactcctga cctcaagtga tccgcatgcc
     5641 tcggactccc aaagtgctgg gattacaggc gtgagccact gcgcccagcc caaattatat
     5701 tttttaataa ctaatacaaa ggtgaagtgg ctcctcccaa gtagcagtgc tgccggggcc
     5761 tggcccaggc ctagccctgg gctcacctct catgctccca agccaaccct gatgctcctg
     5821 gctccagggt agaccttttc cctttagagg ttcagtgtcc agggagctct atgataattg
     5881 tgtgaatgag gtgagcgctg actccatgtg agtattttaa ggcttggagt caaagaatta
     5941 acctaacatg cccaggcaga gtccatgtgt caatggcccg tctccagttt gttctaattt
     6001 ctcaccacgt gtctgtagat ataggaccgc agaccttcgc tttatcatca taatcacatc
     6061 taatgtccat cctaacttac gattcatatt ctgtgcctgg aaacagtccc tgtatttaac
     6121 aatacctaca cataaaaaca atccactgtt acaacttgta tggtcacaaa acattaatga
     6181 tcttctttag accaagcaaa tgttttttta ccataattgt cgtgcaccaa attcacagcc
     6241 agaatcccca agatcaaaaa aaaatttttg cacaatacaa ataataactt aaaatacaca
     6301 tacacacata tacacacact ctcacacgca cttcttacag aactgtgctt ggggaaactc
     6361 catttgttga atgccactga aggcatattt tgctaaactg ctgctccagg tatttttgtt
     6421 tggaaaatct atcacagtag ggcacagctg tttattggat gagcagaaaa gaaagatatg
     6481 cttgtggcaa ccagaaagtt ttacggtctt tcaagttgta taaaatggac ctgcagaaac
     6541 gtttaagtgt tctcaggatg caaagttgga gctgaaatgt gacatcaaac cagttgcaaa
     6601 aagtgtacag cagccatctc ttgaggtcaa acctggtacc cagatatgtg acaaagctac
     6661 tcgaggctag cgagctcgtt taaacaacta gaattacacg gcgatcttgc cgcccttctt
     6721 ggccttaatg agaatctcgc ggatcttgcg ggcgtccaac ttgccggtca gtcctttagg
     6781 cacctcgtcc acgaacacaa caccaccgcg cagcttcttg gcggttgtaa cctggctggc
     6841 cacatagtcc acgatctcct tctcggtcat ggttttaccg tgttccagca cgacgactgc
     6901 ggcgggcagc tcgccggcat cgtcgtcggg caggccggcg accccggcgt cgaagatgtt
     6961 ggggtgttgc agcaggatgc tctccagttc ggctggggct acctggtagc ccttgtattt
     7021 gatcaggctc ttcagccggt ccacgatgaa gaagtgctcg tcctcgtccc agtaggcgat
     7081 gtcgccgctg tgcagccagc cgtccttgtc gatgagagcg tttgtagcct cggggttgtt
     7141 aacgtagccg ctcatgatca tggggccacg gacgcacagc tcgccgcgct ggttcacacc
     7201 cagtgtctta ccggtgtcca agtccaccac cttagcctcg aagaagggca ccaccttgcc
     7261 tactgcgcca ggcttgtcgt ccccttcggg ggtgatcaga atggcgctgg ttgtttctgt
     7321 caggccgtag ccctggcgga tgcctggtag gtggaagcgt ttggccacgg cctcacctac
     7381 ctccttgctg agcggcgccc cgccgctggc gatctcgtgc aagttgctta ggtcgtactt
     7441 gtcgatgaga gtgctcttag cgaagaagct aaatagtgtg ggcaccagca gggcagattg
     7501 aatcttatag tcttgcaagc tgcgcaagaa tagctcctcc tcgaagcggt acatgagcac
     7561 gacccgaaag ccgcagatca agtagcccag cgtggtgaac atgccgaagc cgtggtgaaa
     7621 tggcaccacg ctgaggatag cggtgtcggg gatgatctgg ttgccgaaga tggggtcgcg
     7681 ggcatgactg aatcggacac aagcggtgcg gtgcggtagg gctacgccct tgggcaatcc
     7741 ggtactgcca ctactgttca tgatcagggc gatggttttg tcccggtcga agctctcggg
     7801 cacgaagtcg tactcgttga agccgggtgg caaatgggaa gtcacgaagg tgtacatgct
     7861 ttggaagccc tggtagtcgg tcttgctatc catgatgatg atcttttgta tgatcggtag
     7921 cttcttttgc acgttgagga tcttttgcag ccctttcttg ctcacgaata cgacggtggg
     7981 ctggctgatg cccatgctgt tcagcagctc gcgctcgttg tagatgtcgt tagctggggc
     8041 cacagccaca ccgatgaaca gggcacccaa cacgggcatg aagaactgca agctattctc
     8101 gctgcacacc acgatccgat ggtttgtatt cagcccatag cgcttcatag cttctgccag
     8161 ccgaacgctc atctcgaagt actcggcgta ggtaatgtcc acctcgatat gtgcgtcggt
     8221 aaaggcgatg gtgccgggca ccagggcgta gcgcttcatg gctttgtgca gctgctcgcc
     8281 ggcggtcccg tcttcgagtg ggtagaatgg cgctgggccc ttcttaatgt ttttggcatc
     8341 ttccatggtg gctttaccaa cagtaccgga ttgccaagct tgggctgcag gtcgaaaggc
     8401 ccggagatga ggaagaggag aacagcgcgg cagacgtgcg cttttgaagc gtgcagaatg
     8461 ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct gagcccgccc ctgagcccgc
     8521 ccccggaccc accccttccc agcctctgag cccagaaagc gaaggagcaa agctgctatt
     8581 ggccgctgcc ccaaaggcct acccgcttcc attgctcagc ggtgctgtcc atctgcacga
     8641 gactagtgag acgtgctact tccatttgtc acgtcctgca cgacgcgagc tgcggggcgg
     8701 gggggaactt cctgactagg ggaggagtag aaggtggcgc gaaggggcca ccaaagaacg
     8761 gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg aggccagagg ccacttgtgt
     8821 agcgccaagt gcccagcggg gctgctaaag cgcatgctcc agactgcctt gggaaaagcg
     8881 cctcccctac ccggtagatc tcgatcctct acgccggacg catcgtggcc ggagtactgc
     8941 ggtttatctc gatggctacg agggcagaca gtaagtggat ttaccataat cccttaattg
     9001 tacgcaccgc taaaacgcgt tcagcgcgat cacggcagca gacaggtaaa aatggcaaca
     9061 aaccacccga aaaactgccg caatcgcgcc tgataaattt taaccgtatg aatacctatg
     9121 caaccagagg gtacaggcca cattaccccc acttaatcca ctgaagctgc catttttcat
     9181 ggtttcacca tcccagcgaa gggccatcca gcgtgcgttc ctgtatttcc ggactataaa
     9241 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     9301 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
