LOCUS       Exported               10204 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  22_1.
ACCESSION   22_1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             5618..5755
                     /label=C9orf85 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=71.7"
                     /note="/match_length=25.7"
                     /note="/other=CDS"
     CDS             complement(7551..9203)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(9250..9749)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(9806..10089)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgacttaa taataataat agctaccatt
     4681 tattgagcac ctactctact gtgtgccagg cactgtgcta ggcacttttc gtacaatatc
     4741 taatttaaat cctcacaaca accctgtgag gtaggtatta ttatcatcat tttacatacg
     4801 agggaactga ggctcagaga ggttaaataa ctctatcaca caaccaaaaa tagtagagct
     4861 ggaattcaaa cccaaaacca cctaactcca aatcccatgc tccaatgaat taaagtagag
     4921 aggggagagg gagggccaaa ttcaagacag agtgttggag tgaaatggat atgatatgct
     4981 gataagatat gggaagaagg aatgtaagct cataaatgtt tctagctggt gagcctatgt
     5041 agattgtgat gaagtaacca ggagaggaat tcaggaggaa catacttggt tgggcagatg
     5101 agttgagtct atacggaact gtccatagag atgtccagta ggtaatacca atacagatct
     5161 aaagtctggg ggaagttggg ggttggagct atgtatttgg gaatcaccag catatcagca
     5221 gcaattgagg acatcaggat gaatgactcc attttgggag taaatagaaa ggaaagagaa
     5281 gtgaatcaaa gatggaacga tggaagcact gatactgaca ggcatgagca atcgttgaga
     5341 aattctcaag agagtcggac aagacagagt cagagagaga agtacatgaa acagccctgt
     5401 agtggtggct agaagccatg gactgggttg ggaacaattc tagagctccc agaaggcagg
     5461 caagattgag tcacacttcc aacaaagccc tctgcacacc aaaaccccag gaagtattgt
     5521 ttttctttgt tagtttgctt ttgagacaga atctcactct gtcacccagg ctggagtgcg
     5581 atctcaactc actgcaacct ccgcttgctt cctggattca agcgattctt gtgcctcagc
     5641 ctcctgagta gctgagatta caggcaggtg ccaaaacacc catctaattt ttgtattttt
     5701 agtagagaca gggttttgct atgttggcca ggctggtctc gaactcccga cctcaagtga
     5761 tccacctgtc tcagcctccc aaagtgctgg gattacagac gtgagccacc gcacccgtcc
     5821 aaggaagtat tgttaattga aataaatgcc aaaatgttcc taccttggtt taccccaaaa
     5881 cctggagcta agaacttcat ctcacttttg acaccccagc ccccaaaata tggaagccca
     5941 ggagagccag gagaatttat agcagaggct taaagagaaa gttatgattt gtttaaagta
     6001 gagaataagg tgaaaaataa aacctggtac tctgtctgga agtcctggaa gtctccttgc
     6061 ccaacctcaa ctggcctgtg ggctcctgtg tccttgctct gggatgccat ggtgaatgtg
     6121 aaaacagggg aggttgtgtg tgggggtggg aatggcctgt cggttgcaag gcgagtcctt
     6181 tgctgagccc agcctgagac ccagcttatg ggctttatcc aggtgagaaa atgctgggga
     6241 catgtgtgcg aggtttaagg tctaccagga aggggagaag ggtgtcaggg gaccagcccc
     6301 acaacctagg agaggattag gagggtccag ccctttgggc agggaccagc cctgaggctt
     6361 aaatgaactt gcataggcac ctgtgtatgc atctctgtgc atgtatgcat acatgagtga
     6421 gagagaaaga taaagagagc taaagaggcc cccctccccc cgccgcaggc tgccacacgt
     6481 ggtgcgatcc gattctctct ggaaaggagg agagtctctc atgcatgggt gaagttgggc
     6541 cctccccaga agcctagtga ctcattatgt taacgtgtgg gtgtgggggc tggacatcct
     6601 tgggaagccc tgaggggctg ctgaagcagg gttcctggtc tcctcggagg ctgggcctcc
     6661 cctcgctggg ggctcacaga aagtcaaaca gcccgaaatt cttggctgct ccaggcccag
     6721 ggtaaattgg gggaggaaaa aagaaagaaa agaaatgttt caggcctgga ggtgagatga
     6781 gcctgctggt tagtagggtt aggggtctga aggaaccagc acgcagggca ggcatggggc
     6841 aggggtagag cagagatgct ccctggaagc caccagctgc tcaggcaact gagggggccc
     6901 tctgggcctc tgatctaggc acaggcaggg atggcatgga tggctattgg aggcgactac
     6961 agctttaggt tgcaggactg aggcagcagg cagggggatg gttagtaggg gcagatgaag
     7021 caaatatatt ttggtttcag taatgtgggg taaagtcaag aggtttgcac acgtgcctga
     7081 tggggtgggg ttaatagagg gatgatgtag ctgaataaat ggtgaaaggg agctaaaggg
     7141 gaaacgagag aatgagggag tgagcgtgcc accaaggtca ttgctggggt caactcccaa
     7201 aatgggggtt gcagagggga gtaaggggta gcaccaacaa gtctggtccc agagacaaaa
     7261 ggggtcctgg aagtcctgcg cagggattag tgggcagagg aaacgaactg gggccagagg
     7321 caggttaggg ggctgcagag ccaatcggta ggtgcacccc accctcgcag caccctgcga
     7381 gcagttagcg cacctggatt tagttccttt tatttccctt tcaggtacca gacaaagtaa
     7441 aaaagacgga cggatggaga gaggaacgca gccggctggg gtgggagcgg aatggagctg
     7501 ggggaggggc cctgcactcg aggctagcga gctcgtttaa acaactagaa ttacacggcg
     7561 atcttgccgc ccttcttggc cttaatgaga atctcgcgga tcttgcgggc gtccaacttg
     7621 ccggtcagtc ctttaggcac ctcgtccacg aacacaacac caccgcgcag cttcttggcg
     7681 gttgtaacct ggctggccac atagtccacg atctccttct cggtcatggt tttaccgtgt
     7741 tccagcacga cgactgcggc gggcagctcg ccggcatcgt cgtcgggcag gccggcgacc
     7801 ccggcgtcga agatgttggg gtgttgcagc aggatgctct ccagttcggc tggggctacc
     7861 tggtagccct tgtatttgat caggctcttc agccggtcca cgatgaagaa gtgctcgtcc
     7921 tcgtcccagt aggcgatgtc gccgctgtgc agccagccgt ccttgtcgat gagagcgttt
     7981 gtagcctcgg ggttgttaac gtagccgctc atgatcatgg ggccacggac gcacagctcg
     8041 ccgcgctggt tcacacccag tgtcttaccg gtgtccaagt ccaccacctt agcctcgaag
     8101 aagggcacca ccttgcctac tgcgccaggc ttgtcgtccc cttcgggggt gatcagaatg
     8161 gcgctggttg tttctgtcag gccgtagccc tggcggatgc ctggtaggtg gaagcgtttg
     8221 gccacggcct cacctacctc cttgctgagc ggcgccccgc cgctggcgat ctcgtgcaag
     8281 ttgcttaggt cgtacttgtc gatgagagtg ctcttagcga agaagctaaa tagtgtgggc
     8341 accagcaggg cagattgaat cttatagtct tgcaagctgc gcaagaatag ctcctcctcg
     8401 aagcggtaca tgagcacgac ccgaaagccg cagatcaagt agcccagcgt ggtgaacatg
     8461 ccgaagccgt ggtgaaatgg caccacgctg aggatagcgg tgtcggggat gatctggttg
     8521 ccgaagatgg ggtcgcgggc atgactgaat cggacacaag cggtgcggtg cggtagggct
     8581 acgcccttgg gcaatccggt actgccacta ctgttcatga tcagggcgat ggttttgtcc
     8641 cggtcgaagc tctcgggcac gaagtcgtac tcgttgaagc cgggtggcaa atgggaagtc
     8701 acgaaggtgt acatgctttg gaagccctgg tagtcggtct tgctatccat gatgatgatc
     8761 ttttgtatga tcggtagctt cttttgcacg ttgaggatct tttgcagccc tttcttgctc
     8821 acgaatacga cggtgggctg gctgatgccc atgctgttca gcagctcgcg ctcgttgtag
     8881 atgtcgttag ctggggccac agccacaccg atgaacaggg cacccaacac gggcatgaag
     8941 aactgcaagc tattctcgct gcacaccacg atccgatggt ttgtattcag cccatagcgc
     9001 ttcatagctt ctgccagccg aacgctcatc tcgaagtact cggcgtaggt aatgtccacc
     9061 tcgatatgtg cgtcggtaaa ggcgatggtg ccgggcacca gggcgtagcg cttcatggct
     9121 ttgtgcagct gctcgccggc ggtcccgtct tcgagtgggt agaatggcgc tgggcccttc
     9181 ttaatgtttt tggcatcttc catggtggct ttaccaacag taccggattg ccaagcttgg
     9241 gctgcaggtc gaaaggcccg gagatgagga agaggagaac agcgcggcag acgtgcgctt
     9301 ttgaagcgtg cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag
     9361 cccgcccctg agcccgcccc cggacccacc ccttcccagc ctctgagccc agaaagcgaa
     9421 ggagcaaagc tgctattggc cgctgcccca aaggcctacc cgcttccatt gctcagcggt
     9481 gctgtccatc tgcacgagac tagtgagacg tgctacttcc atttgtcacg tcctgcacga
     9541 cgcgagctgc ggggcggggg ggaacttcct gactagggga ggagtagaag gtggcgcgaa
     9601 ggggccacca aagaacggag ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg
     9661 ccagaggcca cttgtgtagc gccaagtgcc cagcggggct gctaaagcgc atgctccaga
     9721 ctgccttggg aaaagcgcct cccctacccg gtagatctcg atcctctacg ccggacgcat
     9781 cgtggccgga gtactgcggt ttatctcgat ggctacgagg gcagacagta agtggattta
     9841 ccataatccc ttaattgtac gcaccgctaa aacgcgttca gcgcgatcac ggcagcagac
     9901 aggtaaaaat ggcaacaaac cacccgaaaa actgccgcaa tcgcgcctga taaattttaa
     9961 ccgtatgaat acctatgcaa ccagagggta caggccacat tacccccact taatccactg
    10021 aagctgccat ttttcatggt ttcaccatcc cagcgaaggg ccatccagcg tgcgttcctg
    10081 tatttccgga ctataaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    10141 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10201 cttc
//
