LOCUS       Exported                8141 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T10.
ACCESSION   T10
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4658)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=43.9"
                     /note="/other=misc_feature"
     CDS             complement(5488..7140)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7187..7686)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(7743..8026)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactccg cagcaggcag gaggcgtcct
     4681 cttcagcgta gctctccacc tctacccgga acacaccctc tcacagacgt accaatgtta
     4741 tttttataat ttcatggatt tagttataca taccttaata gttttataaa attgttgaca
     4801 tttcaggcaa atttggccaa tattatcatt gaattttctg tgttggattt cctctaggat
     4861 ttcgccagtt cctacaacgt gcagtagggc ggcggtagct cttgtgtctg tggactctgc
     4921 tcagctgtgt ccgtaggagt cggatgtgtc tgtgctttat tatggccttg tttatatatc
     4981 actgaggtat actatgccat gtaaatagac tattttttat aatctttaca tgctggttta
     5041 aattcagaag gaaatagatc aaggaaatat atatattttc ttctaaaact tattaaattc
     5101 gtgtgacaaa taatcatttt catcttggca gcaaaaagtt ctcagtgacc tattttgtgg
     5161 tgtttctttt tgaaaagaaa agctgaaata ttattaaatg ctagtatgtt tctgcccatt
     5221 atgaaagatg aaataaagta ttcaaaatat taacattttc ataaatataa ggatgtatta
     5281 ttgagaagta agttgaaggg cttataagga aaaatgtttt ataactgagt aatatattaa
     5341 gagaattgtc atggttcata aatcacatta tgctaatctg aaatttctta cataaaaatg
     5401 aagtgtctta tgtttatttt aattgctgtt gtaacttact catgaaacag taactcgagg
     5461 ctagcgagct cgtttaaaca actagaatta cacggcgatc ttgccgccct tcttggcctt
     5521 aatgagaatc tcgcggatct tgcgggcgtc caacttgccg gtcagtcctt taggcacctc
     5581 gtccacgaac acaacaccac cgcgcagctt cttggcggtt gtaacctggc tggccacata
     5641 gtccacgatc tccttctcgg tcatggtttt accgtgttcc agcacgacga ctgcggcggg
     5701 cagctcgccg gcatcgtcgt cgggcaggcc ggcgaccccg gcgtcgaaga tgttggggtg
     5761 ttgcagcagg atgctctcca gttcggctgg ggctacctgg tagcccttgt atttgatcag
     5821 gctcttcagc cggtccacga tgaagaagtg ctcgtcctcg tcccagtagg cgatgtcgcc
     5881 gctgtgcagc cagccgtcct tgtcgatgag agcgtttgta gcctcggggt tgttaacgta
     5941 gccgctcatg atcatggggc cacggacgca cagctcgccg cgctggttca cacccagtgt
     6001 cttaccggtg tccaagtcca ccaccttagc ctcgaagaag ggcaccacct tgcctactgc
     6061 gccaggcttg tcgtcccctt cgggggtgat cagaatggcg ctggttgttt ctgtcaggcc
     6121 gtagccctgg cggatgcctg gtaggtggaa gcgtttggcc acggcctcac ctacctcctt
     6181 gctgagcggc gccccgccgc tggcgatctc gtgcaagttg cttaggtcgt acttgtcgat
     6241 gagagtgctc ttagcgaaga agctaaatag tgtgggcacc agcagggcag attgaatctt
     6301 atagtcttgc aagctgcgca agaatagctc ctcctcgaag cggtacatga gcacgacccg
     6361 aaagccgcag atcaagtagc ccagcgtggt gaacatgccg aagccgtggt gaaatggcac
     6421 cacgctgagg atagcggtgt cggggatgat ctggttgccg aagatggggt cgcgggcatg
     6481 actgaatcgg acacaagcgg tgcggtgcgg tagggctacg cccttgggca atccggtact
     6541 gccactactg ttcatgatca gggcgatggt tttgtcccgg tcgaagctct cgggcacgaa
     6601 gtcgtactcg ttgaagccgg gtggcaaatg ggaagtcacg aaggtgtaca tgctttggaa
     6661 gccctggtag tcggtcttgc tatccatgat gatgatcttt tgtatgatcg gtagcttctt
     6721 ttgcacgttg aggatctttt gcagcccttt cttgctcacg aatacgacgg tgggctggct
     6781 gatgcccatg ctgttcagca gctcgcgctc gttgtagatg tcgttagctg gggccacagc
     6841 cacaccgatg aacagggcac ccaacacggg catgaagaac tgcaagctat tctcgctgca
     6901 caccacgatc cgatggtttg tattcagccc atagcgcttc atagcttctg ccagccgaac
     6961 gctcatctcg aagtactcgg cgtaggtaat gtccacctcg atatgtgcgt cggtaaaggc
     7021 gatggtgccg ggcaccaggg cgtagcgctt catggctttg tgcagctgct cgccggcggt
     7081 cccgtcttcg agtgggtaga atggcgctgg gcccttctta atgtttttgg catcttccat
     7141 ggtggcttta ccaacagtac cggattgcca agcttgggct gcaggtcgaa aggcccggag
     7201 atgaggaaga ggagaacagc gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc
     7261 ctccggagga ccttcgggcg cccgccccgc ccctgagccc gcccctgagc ccgcccccgg
     7321 acccacccct tcccagcctc tgagcccaga aagcgaagga gcaaagctgc tattggccgc
     7381 tgccccaaag gcctacccgc ttccattgct cagcggtgct gtccatctgc acgagactag
     7441 tgagacgtgc tacttccatt tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga
     7501 acttcctgac taggggagga gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg
     7561 gttggcgcct accggtggat gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc
     7621 aagtgcccag cggggctgct aaagcgcatg ctccagactg ccttgggaaa agcgcctccc
     7681 ctacccggta gatctcgatc ctctacgccg gacgcatcgt ggccggagta ctgcggttta
     7741 tctcgatggc tacgagggca gacagtaagt ggatttacca taatccctta attgtacgca
     7801 ccgctaaaac gcgttcagcg cgatcacggc agcagacagg taaaaatggc aacaaaccac
     7861 ccgaaaaact gccgcaatcg cgcctgataa attttaaccg tatgaatacc tatgcaacca
     7921 gagggtacag gccacattac ccccacttaa tccactgaag ctgccatttt tcatggtttc
     7981 accatcccag cgaagggcca tccagcgtgc gttcctgtat ttccggacta taaaaggatc
     8041 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     8101 cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
