LOCUS       Exported                9415 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  12_1.
ACCESSION   12_1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..9415
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             complement(6762..8414)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(8461..8960)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(9017..9300)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactaac tactttatgc atgaaaaaag
     4681 tctgtttcac tgatatgaaa tgttaatgca ctatttaatt tttttctctt tgttgcttca
     4741 aaatgaggtt ggtttaagat aataatagga catctgcaga taagtcatcc tctacatgaa
     4801 ggtgacagac tgttggcagt ttcaaaataa tcaagaagag aaatatcctt agcaaagaga
     4861 tgactttggg gatcatttga ggaatactaa ctctgttgca ttaatgcttc aaaaaatcat
     4921 caaatgattc atgggggcct gatttgcatt tgaaaaatgt ttgaaattag agtctcattt
     4981 gtttcaggaa tgcagctacc tgagtttttt gtctcggcaa agtcacaaag cccatatact
     5041 cacattgtgt gtctatattt gccaattaat tctaaacttc taggaaatat gccctctctt
     5101 aaaggagaat tttttttaaa tctctgagaa atgagattct gagtttattt cagctaaaag
     5161 gttgcaattc ttctgaagat atctcaaata taaggttgaa agttaagtgt taataatttt
     5221 tgtgaattta tacacaccta aacgttaagt acacaaatat tttatttgtt ttacaaaaaa
     5281 ggaataagta atttataaat taagaagtta cctataaaaa taaaaagata acaaccctat
     5341 catatagctt atttttaaat tacctgaaaa acgatattct acactgtttc ctttttgagt
     5401 ctgagttttc aaactgttac ttctcccata tttctcaatc catttcactc agttgcacag
     5461 tcttttaaac cctgtaattg tcataccaaa gtttcttttt aaaaaaaaat tactttaaat
     5521 gcttagttta ttcaaagagc gatccaataa tataaaagga acatgtgtta aacacaataa
     5581 aattttaaat ggctctaaat caagcacatc aagagtatac aagtcttaaa ggctttttaa
     5641 tacatactct tttcccatct atgtaaccca acttgcacat ttcagctgca tgtggtgaat
     5701 atgcatcata tatttacttt aagaggtaag attttacttg caaaatacat gtgcaaatta
     5761 gggatccatc agttgatgga agagatggac tctagaatat tatttcttgt ggttattact
     5821 cctttacaaa gcactttcgt ctcacttgat cctcataagg aaactaaggc tcagaatgag
     5881 tagagctggg ttcagaatct agctcttcta actccaagcc atctcctctt tccactgcag
     5941 gaaactgcct cttttgtcag tgaaataata gaaagattgt gttagttaag tgataactgt
     6001 catttgtttg aaaatgttcg agactgaaca aatagcattt aaactgctgg catatagatg
     6061 agatattgta cttttgtgca atgtttatta cctttgatta aattgtaatg tgaagctttt
     6121 actaggtgaa tagttcatta tgtagtggag gcttcgtggt tgtccattga attgtcacag
     6181 caaaatctat aagtttcttc aattctacaa gatagatcca tatacctttg atcacttgga
     6241 gactcttttt ttgctggttt ctagataact caggtaaatc agacctttac agagtacagg
     6301 gctaggtgaa agaattactg aaaaatcacc ttgaaaatcc gaagggctga tatacccttt
     6361 atgttcctga ctgatgcgca gaacctgggg gaaatctaca gcaatataca ggttgcaatg
     6421 ctgataacac aacagcaatc ctctcctcta cgtggactta ctgttgtttt tttaattatt
     6481 attggaatgg gattttagaa aatagaagtt acctttgtgt gtgttttagg gaaggtagag
     6541 aagaatctgc tctttctctg aatactgttt tgaccccagg caggaccttg gaaaggccaa
     6601 aacattaaca gtagtacttc tgttcactga agagttatgt tacatgaaga taaaatggtt
     6661 ttgtcgtgtt tattattgta ttttgtgttg atataaataa acatggtaat ttaaacaatg
     6721 aaccttactc gaggctagcg agctcgttta aacaactaga attacacggc gatcttgccg
     6781 cccttcttgg ccttaatgag aatctcgcgg atcttgcggg cgtccaactt gccggtcagt
     6841 cctttaggca cctcgtccac gaacacaaca ccaccgcgca gcttcttggc ggttgtaacc
     6901 tggctggcca catagtccac gatctccttc tcggtcatgg ttttaccgtg ttccagcacg
     6961 acgactgcgg cgggcagctc gccggcatcg tcgtcgggca ggccggcgac cccggcgtcg
     7021 aagatgttgg ggtgttgcag caggatgctc tccagttcgg ctggggctac ctggtagccc
     7081 ttgtatttga tcaggctctt cagccggtcc acgatgaaga agtgctcgtc ctcgtcccag
     7141 taggcgatgt cgccgctgtg cagccagccg tccttgtcga tgagagcgtt tgtagcctcg
     7201 gggttgttaa cgtagccgct catgatcatg gggccacgga cgcacagctc gccgcgctgg
     7261 ttcacaccca gtgtcttacc ggtgtccaag tccaccacct tagcctcgaa gaagggcacc
     7321 accttgccta ctgcgccagg cttgtcgtcc ccttcggggg tgatcagaat ggcgctggtt
     7381 gtttctgtca ggccgtagcc ctggcggatg cctggtaggt ggaagcgttt ggccacggcc
     7441 tcacctacct ccttgctgag cggcgccccg ccgctggcga tctcgtgcaa gttgcttagg
     7501 tcgtacttgt cgatgagagt gctcttagcg aagaagctaa atagtgtggg caccagcagg
     7561 gcagattgaa tcttatagtc ttgcaagctg cgcaagaata gctcctcctc gaagcggtac
     7621 atgagcacga cccgaaagcc gcagatcaag tagcccagcg tggtgaacat gccgaagccg
     7681 tggtgaaatg gcaccacgct gaggatagcg gtgtcgggga tgatctggtt gccgaagatg
     7741 gggtcgcggg catgactgaa tcggacacaa gcggtgcggt gcggtagggc tacgcccttg
     7801 ggcaatccgg tactgccact actgttcatg atcagggcga tggttttgtc ccggtcgaag
     7861 ctctcgggca cgaagtcgta ctcgttgaag ccgggtggca aatgggaagt cacgaaggtg
     7921 tacatgcttt ggaagccctg gtagtcggtc ttgctatcca tgatgatgat cttttgtatg
     7981 atcggtagct tcttttgcac gttgaggatc ttttgcagcc ctttcttgct cacgaatacg
     8041 acggtgggct ggctgatgcc catgctgttc agcagctcgc gctcgttgta gatgtcgtta
     8101 gctggggcca cagccacacc gatgaacagg gcacccaaca cgggcatgaa gaactgcaag
     8161 ctattctcgc tgcacaccac gatccgatgg tttgtattca gcccatagcg cttcatagct
     8221 tctgccagcc gaacgctcat ctcgaagtac tcggcgtagg taatgtccac ctcgatatgt
     8281 gcgtcggtaa aggcgatggt gccgggcacc agggcgtagc gcttcatggc tttgtgcagc
     8341 tgctcgccgg cggtcccgtc ttcgagtggg tagaatggcg ctgggccctt cttaatgttt
     8401 ttggcatctt ccatggtggc tttaccaaca gtaccggatt gccaagcttg ggctgcaggt
     8461 cgaaaggccc ggagatgagg aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt
     8521 gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga gcccgcccct
     8581 gagcccgccc ccggacccac cccttcccag cctctgagcc cagaaagcga aggagcaaag
     8641 ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg tgctgtccat
     8701 ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg acgcgagctg
     8761 cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga aggggccacc
     8821 aaagaacgga gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc
     8881 acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag actgccttgg
     8941 gaaaagcgcc tcccctaccc ggtagatctc gatcctctac gccggacgca tcgtggccgg
     9001 agtactgcgg tttatctcga tggctacgag ggcagacagt aagtggattt accataatcc
     9061 cttaattgta cgcaccgcta aaacgcgttc agcgcgatca cggcagcaga caggtaaaaa
     9121 tggcaacaaa ccacccgaaa aactgccgca atcgcgcctg ataaatttta accgtatgaa
     9181 tacctatgca accagagggt acaggccaca ttacccccac ttaatccact gaagctgcca
     9241 tttttcatgg tttcaccatc ccagcgaagg gccatccagc gtgcgttcct gtatttccgg
     9301 actataaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     9361 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttc
//
