LOCUS       AD000_(CHIP-K30A-myc)   6338 bp ds-DNA     circular     30-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..996
                     /label="CHIP-myc-HIS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             1..996
                     /label="Translation 1-996"
                     /translation="MKGKEEKEGGARLGAGGGSPEKSPSAQELAEQGNRLFVGRKYPEAAACYGRAITRNPLVAVYYTNRALCYLKMQQHEQALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAKEQRLNFGDDIPSALRIAKKKRWNSIEERRIHQESELHSYLSRLIAAERERELEECQRNHEGDEDDSHVRAQQACIEAKHDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGWVEDYYLEGPRFEQKLISEEDLNMHTGHHHHHH*"
     misc_feature    76..177
                     /label="TPR1 (CHIP)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    88..90
                     /label="K30A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    178..279
                     /label="TPR2 (CHIP)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    283..381
                     /label="TPR3 (CHIP)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    382..675
                     /label="mixed charged"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    676..900
                     /label="UBOX (CHIP)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             931..960
                     /label="MYC tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    976..993
                     /label="HIS tag"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      1309..1615
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             2134..2925
                     /label="Kan/neoR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(3267..3287)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_binding    complement(3293..3315)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(3319..3349)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      complement(3655..4337)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(4435..5094)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    5429..5428
                     /label="source"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        6043..6246
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     6291..6310
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 atgaagggca aggaggagaa ggagggcggc gcacggctgg gcgctggcgg cggaagcccc
       61 gagaagagcc cgagcgcgca ggagctcGCG gagcagggca atcgtctgtt cgtgggccga
      121 aagtacccgg aggcggcggc ctgctacggc cgcgcgatca cccggaaccc gctggtggcc
      181 gtgtattaca ccaaccgggc cttgtgctac ctgaagatgc agcagcacga gcaggccctg
      241 gccgactgcc ggcgcgccct ggagctggac gggcagtctg tgaaggcgca cttcttcctg
      301 gggcagtgcc agctggagat ggagagctat gatgaggcca tcgccaatct gcagcgagct
      361 tacagcctgg ccaaggagca gcggctgaac ttcggggacg acatccccag cgctcttcga
      421 atcgcgaaga agaagcgctg gaacagcatt gaggagcggc gcatccacca ggagagcgag
      481 ctgcactcct acctctccag gctcattgcc gcggagcgtg agagggagct ggaagagtgc
      541 cagcgaaacc acgagggtga tgaggacgac agccacgtcc gggcccagca ggcctgcatt
      601 gaggccaagc acgacaagta catggcggac atggacgagc ttttttctca ggtggatgag
      661 aagaggaaga agcgagacat ccccgactac ctgtgtggca agatcagctt tgagctgatg
      721 cgggagccgt gcatcacgcc cagtggcatc acctacgacc gcaaggacat cgaggagcac
      781 ctgcagcgtg tgggtcattt tgaccccgtg acccggagcc ccctgaccca ggaacagctc
      841 atccccaact tggctatgaa ggaggttatt gacgcattca tctctgagaa tggctgggtg
      901 gaggactact atctagaggg cccgcggttc gaacaaaaac tcatctcaga agaggatctg
      961 aatatgcata ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg
     1021 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     1081 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     1141 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     1201 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     1261 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     1321 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     1381 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     1441 aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     1501 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     1561 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     1621 aaccctatct cggtctattc ttttgattta taagggattt tggggatttc ggcctattgg
     1681 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     1741 agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat
     1801 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     1861 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     1921 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     1981 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     2041 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     2101 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     2161 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     2221 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     2281 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     2341 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     2401 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     2461 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     2521 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     2581 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     2641 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     2701 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     2761 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     2821 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     2881 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     2941 tcgcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     3001 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     3061 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     3121 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3181 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     3241 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     3301 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     3361 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3421 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3481 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3541 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3601 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3661 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3721 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3781 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3841 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     3901 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3961 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4021 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4081 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     4141 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4201 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4261 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4321 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4381 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4441 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4501 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4561 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4621 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4681 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4741 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4801 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4861 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4921 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4981 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     5041 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     5101 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     5161 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5221 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5281 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5341 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5401 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     5461 tcgactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     5521 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     5581 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     5641 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     5701 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     5761 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     5821 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggacta tttacggtaa
     5881 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     5941 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     6001 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     6061 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     6121 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     6181 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     6241 agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact
     6301 cactataggg agacccaagc tggctagtta agcttgcc
//