LOCUS       AD100_(CHIP-myc)        6338 bp ds-DNA     circular     08-NOV-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(13..695)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(793..1452)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1787..1786
                     /label="source"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        2401..2604
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     2649..2668
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2697..3692
                     /label="CHIP-myc-HIS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2697..3692
                     /label="Translation 2697-3692"
                     /translation="MKGKEEKEGGARLGAGGGSPEKSPSAQELKEQGNRLFVGRKYPEAAACYGRAITRNPLVAVYYTNRALCYLKMQQHEQALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAKEQRLNFGDDIPSALRIAKKKRWNSIEERRIHQESELHSYLSRLIAAERERELEECQRNHEGDEDDSHVRAQQACIEAKHDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGWVEDYYLEGPRFEQKLISEEDLNMHTGHHHHHH*"
     CDS             2697..3692
                     /label="Translation 2697-3692"
                     /translation="MKGKEEKEGGARLGAGGGSPEKSPSAQELKEQGNRLFVGRKYPEAAACYGRAITRNPLVAVYYTNRALCYLKMQQHEQALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAKEQRLNFGDDIPSALRIAKKKRWNSIEERRIHQESELHSYLSRLIAAERERELEECQRNHEGDEDDSHVRAQQACIEAKHDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGWVEDYYLEGPRFEQKLISEEDLNMHTGHHHHHH*"
     misc_feature    2697..3692
                     /label="AD100 (CHIP-myc) [2697-3692]"
     misc_feature    2772..2873
                     /label="TPR1 (CHIP)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2874..2975
                     /label="TPR2 (CHIP)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2979..3077
                     /label="TPR3 (CHIP)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    3078..3371
                     /label="mixed charged"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3372..3596
                     /label="UBOX (CHIP)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             3627..3656
                     /label="MYC tag"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3672..3689
                     /label="HIS tag"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     rep_origin      4005..4311
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             4830..5621
                     /label="Kan/neoR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(5963..5983)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_binding    complement(5989..6011)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(6015..6045)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
       61 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      121 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      181 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
      241 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
      301 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
      361 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
      421 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
      481 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
      541 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
      601 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
      661 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
      721 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
      781 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
      841 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
      901 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
      961 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     1021 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     1081 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     1141 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     1201 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     1261 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     1321 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     1381 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     1441 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     1501 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     1561 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     1621 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     1681 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     1741 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     1801 tctcccgatc ccctatggtc gactctcagt acaatctgct ctgatgccgc atagttaagc
     1861 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     1921 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     1981 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     2041 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     2101 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     2161 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     2221 gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     2281 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     2341 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     2401 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     2461 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     2521 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     2581 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
     2641 atcgaaatta atacgactca ctatagggag acccaagctg gctagttaag cttgccatga
     2701 agggcaagga ggagaaggag ggcggcgcac ggctgggcgc tggcggcgga agccccgaga
     2761 agagcccgag cgcgcaggag ctcaaggagc agggcaatcg tctgttcgtg ggccgaaagt
     2821 acccggaggc ggcggcctgc tacggccgcg cgatcacccg gaacccgctg gtggccgtgt
     2881 attacaccaa ccgggccttg tgctacctga agatgcagca gcacgagcag gccctggccg
     2941 actgccggcg cgccctggag ctggacgggc agtctgtgaa ggcgcacttc ttcctggggc
     3001 agtgccagct ggagatggag agctatgatg aggccatcgc caatctgcag cgagcttaca
     3061 gcctggccaa ggagcagcgg ctgaacttcg gggacgacat ccccagcgct cttcgaatcg
     3121 cgaagaagaa gcgctggaac agcattgagg agcggcgcat ccaccaggag agcgagctgc
     3181 actcctacct ctccaggctc attgccgcgg agcgtgagag ggagctggaa gagtgccagc
     3241 gaaaccacga gggtgatgag gacgacagcc acgtccgggc ccagcaggcc tgcattgagg
     3301 ccaagcacga caagtacatg gcggacatgg acgagctttt ttctcaggtg gatgagaaga
     3361 ggaagaagcg agacatcccc gactacctgt gtggcaagat cagctttgag ctgatgcggg
     3421 agccgtgcat cacgcccagt ggcatcacct acgaccgcaa ggacatcgag gagcacctgc
     3481 agcgtgtggg tcattttgac cccgtgaccc ggagccccct gacccaggaa cagctcatcc
     3541 ccaacttggc tatgaaggag gttattgacg cattcatctc tgagaatggc tgggtggagg
     3601 actactatct agagggcccg cggttcgaac aaaaactcat ctcagaagag gatctgaata
     3661 tgcataccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg
     3721 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     3781 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     3841 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     3901 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     3961 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     4021 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4081 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4141 ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4201 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4261 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4321 ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa
     4381 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     4441 agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca
     4501 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4561 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     4621 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     4681 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     4741 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     4801 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     4861 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     4921 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     4981 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     5041 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     5101 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     5161 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     5221 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     5281 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     5341 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     5401 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     5461 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     5521 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     5581 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc
     5641 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     5701 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     5761 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     5821 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5881 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     5941 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6001 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6061 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6121 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6181 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6241 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6301 ggaaagaaca tgtgagcaaa aggccagcaa aaggccag
//