LOCUS       2200_to_460_of_C        6495 bp    DNA     circular SYN 19-Oct-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..453
                     /label="IR DR (L)"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="0.667,0.000,0.000,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="IR DR (L)"
                     /mv_font_color="1.000,1.000,1.000,1.000"
                     /mv_label_position=Center
                     /mv_visible=1
     frag            461..975
                     /date="19-Oct-2020"
                     /left_cutter="ClaI (2)"
                     /left_end="5CG"
                     /note="3 to 517 of mpGK promoter"
                     /right_cutter="NotI (517)"
                     /right_end="5GGCC"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.413,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     frag            <465..>973
                     /date="13-Sep-2020"
                     /note="360 to 870 of pBP_Capricious4 copy"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.467,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     regulatory      <465..>973
                     /regulatory_class=""
                     /label="mPGK"
                     /mv_symbol_type=HollowArrow
                     /mv_fill_color="0.600,1.000,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="mPGK"
     frag            join(976..6495,1..975)
                     /date="19-Oct-2020"
                     /left_cutter="NotI (2199)"
                     /left_end="5GGCC"
                     /left_join="keep end"
                     /note="2200 to 460 of CamVNGI-X plus 3 to 517 of mpGK
                     promoter"
                     /right_cutter="NotI (517)"
                     /right_end="5GGCC"
                     /right_join="keep end"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.413,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     frag            join(976..6495,1..460)
                     /date="19-Oct-2020"
                     /left_cutter="NotI (2199)"
                     /left_end="5GGCC"
                     /note="2200 to 460 of CamVNGI-X"
                     /right_cutter="ClaI (460)"
                     /right_end="5CG"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.413,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     frag            join(976..>6495,1..>460)
                     /date="29-Jul-2020"
                     /left_cutter="NotI (3)"
                     /left_end="5GGCC"
                     /left_join="keep end"
                     /note="4 to 1363 of mVenus-P2A-NRasG12V plus 2207 to 2199
                     of pCaI-X"
                     /right_cutter="NotI (2199)"
                     /right_end="5GGCC"
                     /right_join="keep end"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.426,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     frag            976..2335
                     /date="29-Jul-2020"
                     /left_cutter="NotI (3)"
                     /left_end="5GGCC"
                     /note="4 to 1363 of mVenus-P2A-NRasG12V"
                     /right_cutter="AgeI (1363)"
                     /right_end="5CCGG"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.426,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     frag            982..>984
                     /date="27-Jul-2020"
                     /note="4751 to 5572 of NLS_mVenus_P2A_Lenti [Split]"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.453,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     CDS             982..1698
                     /label="mVenus"
                     /mv_fill_color="0.275,1.000,0.502,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="mVenus"
     frag            <985..>1698
     frag            <1699..1764
     CDS             1699..1707
                     /label="GSG linker"
                     /mv_fill_color="0.267,0.302,1.000,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="GSG linker"
     CDS             1708..1764
                     /label="P2A"
                     /mv_symbol_type=HollowArrow
                     /mv_fill_color="0.267,0.311,1.000,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="P2A"
                     /mv_fontsize=10.000000
                     /mv_show_residues_if_room=ShowAsGraphics
     frag            1765..2334
                     /date="27-Jul-2020"
                     /left_end="0"
                     /note="1 to 570 of Untitled"
                     /right_end="0"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.453,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     gene            1765..2334
                     /label="NRAS G12V"
                     /mv_symbol_type=HollowArrow
                     /mv_fill_color="0.000,0.000,0.867,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="NRAS G12V"
                     /mv_font_color="1.000,1.000,1.000,1.000"
                     /mv_label_position=Center
     frag            join(2336..>6495,1..>460)
                     /date="29-Jul-2020"
                     /left_cutter="AgeI (2206)"
                     /left_end="5CCGG"
                     /note="2207 to 2199 of pCaI-X"
                     /right_cutter="NotI (2199)"
                     /right_end="5GGCC"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.426,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     frag            join(2336..>6495,1..>460)
                     /date="27-Jul-2020"
                     /left_cutter="NotI (2823)"
                     /left_end="5GGCC"
                     /left_join="keep end"
                     /right_cutter="NotI (3735)"
                     /right_end="5GGCC"
                     /right_join="keep end"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.439,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     frag            join(<2336..6495,<1..460)
                     /date="27-Jul-2020"
                     /left_cutter="NotI (2823)"
                     /left_end="5GGCC"
                     /right_cutter="ClaI (460)"
                     /right_end="5CG"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.439,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     misc_feature    2377..2951
                     /label="IRES"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="0.200,1.000,1.000,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="IRES"
                     /mv_label_position=Center
                     /mv_visible=1
     polyA_signal    3204..3431
                     /label="pA"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.769,0.000,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="pA"
                     /mv_font_color="1.000,1.000,1.000,1.000"
                     /mv_label_position=Center
                     /mv_visible=1
     misc_feature    3526..3830
                     /label="IR DR (R)"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="0.667,0.000,0.000,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="IR DR (R)"
                     /mv_font_color="1.000,1.000,1.000,1.000"
                     /mv_label_position=Center
                     /mv_visible=1
     gene            complement(5042..5701)
                     /label="AmpR"
                     /mv_symbol_type=HollowArrow
                     /mv_fill_color="0.467,0.598,0.467,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="AmpR"
                     /mv_font_color="1.000,1.000,1.000,1.000"
                     /mv_label_position=Center
BASE COUNT  1650 A  1630 C  1688 G  1527 T  0 OTHER
ORIGIN
        1 AATTCGAGCT CGGTACCCGC AGTTGAAGTC GGAAGTTTAC ATACACTTAA GTTGGAGTCA
       61 TTAAAACTCG TTTTTCAACT ACACCACAAA TTTCTTGTTA ACAAACAATA GTTTTGGCAA
      121 GTCAGTTAGG ACATCTACTT TGTGCATGAC ACAAGTCATT TTTCCAACAA TTGTTTACAG
      181 ACAGATTATT TCACTTATAA TTCACTGTAT CACAATTCCA GTGGGTCAGA AGTTTACATA
      241 CACTAAGTTG ACTGTGCCTT TAAACAGCTT GGAAAATTCC AGAAAATGAT GTCATGGCTT
      301 TAGAAGCTTC TGATAGACTA ATTGACATCA TTTGAGTCAA TTGGAGGTGT ACCTGTGGAT
      361 GTATTTCAAG GCGATGTACG GGCCAGATAT ACGCGTTGAC ATTGATTATT GACTAGTAAC
      421 GGCCGCCAGT GTGCTGGAAT TCTGCAGATA TCAAGCTTAT CGATTCTACC GGGTAGGGGA
      481 GGCGCTTTTC CCAAGGCAGT CTGGAGCATG CGCTTTAGCA GCCCCGCTGG GCACTTGGCG
      541 CTACACAAGT GGCCTCTGGC CTCGCACACA TTCCACATCC ACCGGTAGGC GCCAACCGGC
      601 TCCGTTCTTT GGTGGCCCCT TCGCGCCACC TTCTACTCCT CCCCTAGTCA GGAAGTTCCC
      661 CCCCGCCCCG CAGCTCGCGT CGTGCAGGAC GTGACAAATG GAAGTAGCAC GTCTCACTAG
      721 TCTCGTGCAG ATGGACAGCA CCGCTGAGCA ATGGAAGCGG GTAGGCCTTT GGGGCAGCGG
      781 CCAATAGCAG CTTTGCTCCT TCGCTTTCTG GGCTCAGAGG CTGGGAAGGG GTGGGTCCGG
      841 GGGCGGGCTC AGGGGCGGGC TCAGGGGCGG GGCGGGCGCC CGAAGGTCCT CCGGAGGCCC
      901 GGCATTCTGC ACGCTTCAAA AGCGCACGTC TGCCGCGCTG TTCTCCTCTT CCTCATCTCC
      961 GGGCCTTTCG ACCGCGGCCG CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG
     1021 CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG
     1081 GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGC TGATCTGCAC CACCGGCAAG
     1141 CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGGGCT ACGGCCTGCA GTGCTTCGCC
     1201 CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC
     1261 GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG
     1321 AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG
     1381 GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC
     1441 ACCGCCGACA AGCAGAAGAA CGGCATCAAG GCCAACTTCA AGATCCGCCA CAACATCGAG
     1501 GACGGCGGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC
     1561 GTGCTGCTGC CCGACAACCA CTACCTGAGC TACCAGTCCA AGCTGAGCAA AGACCCCAAC
     1621 GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC
     1681 ATGGACGAGC TGTACAAAGG AAGCGGAGCT ACTAACTTCA GCCTGCTGAA GCAGGCTGGA
     1741 GACGTGGAGG AGAACCCTGG ACCTATGACT GAGTACAAAC TGGTGGTGGT TGGAGCAGTT
     1801 GGTGTTGGGA AAAGCGCACT GACAATCCAG CTAATCCAGA ACCACTTTGT AGATGAATAT
     1861 GATCCCACCA TAGAGGATTC TTACAGAAAA CAAGTGGTTA TAGATGGTGA AACCTGTTTG
     1921 TTGGACATAC TGGATACAGC TGGACAAGAA GAGTACAGTG CCATGAGAGA CCAATACATG
     1981 AGGACAGGCG AAGGCTTCCT CTGTGTATTT GCCATCAATA ATAGCAAGTC ATTTGCGGAT
     2041 ATTAACCTCT ACAGGGAACA GATTAAGCGA GTAAAAGACT CGGATGATGT ACCTATGGTG
     2101 CTAGTGGGAA ACAAGTGTGA TTTGCCAACA AGGACAGTTG ATACAAAACA AGCCCACGAA
     2161 CTGGCCAAGA GTTACGGGAT TCCATTCATT GAAACCTCAG CCAAGACCAG ACAGGGTGTT
     2221 GAAGATGCTT TTTACACACT GGTAAGAGAA ATACGCCAGT ACCGAATGAA AAAACTCAAC
     2281 AGCAGTGATG ATGGGACTCA GGGTTGTATG GGATTGCCAT GTGTGGTGAT GTAAACCGGT
     2341 AGGCCTCGTA CGCTTAATTA ACGGATCCGG AATTCCGCCC CTCTCCCTCC CCCCCCCCTA
     2401 ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC GTTTGTCTAT ATGTTATTTT
     2461 CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA ACCTGGCCCT GTCTTCTTGA
     2521 CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG TTGAATGTCG
     2581 TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC AACGTCTGTA GCGACCCTTT
     2641 GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG CGGCCAAAAG CCACGTGTAT
     2701 AAGATACACC TGCAAAGGCG GCACAACCCC AGTGCCACGT TGTGAGTTGG ATAGTTGTGG
     2761 AAAGAGTCAA ATGGCTCTCC TCAAGCGTAT TCAACAAGGG GCTGAAGGAT GCCCAGAAGG
     2821 TACCCCATTG TATGGGATCT GATCTGGGGC CTCGGTGCAC ATGCTTTACA TGTGTTTAGT
     2881 CGAGGTTAAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA CGTGGTTTTC CTTTGAAAAA
     2941 CACGATGATA AGCTTGATCA CGCGTCTCGA GATATCAGTG GTCCAGGCTC TAGTTTTGAC
     3001 TCAACAATAT CACCAGCTGA AGCCTATAGA GTACGAGCCA TAGATAAAAT AAAAGATTTT
     3061 ATTTAGTCTC CAGAAAAAGG GGGGAATGAA AGACCCCACC TGTAGGTTTG GCAAGCTAGC
     3121 ATTCCCGGGA AAACTCAACA GCAGTGATGA TGGGACTCAG GGTTGTATGG GATTGCCATG
     3181 TGTGGTGATG TAAGGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG
     3241 CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA
     3301 AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT
     3361 GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT
     3421 GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCCATCAC AAAGCTCTGA CCTCAATCCT
     3481 ATAGAAAGGA GGAATGAGCC AAAATTCACC CAACTTATTG TGGGAAGCTT GTGGAAGGCT
     3541 ACTCGAAATG TTTGACCCAA GTTAAACAAT TTAAAGGCAA TGCTACCAAA TACTAATTGA
     3601 GTGTATGTTA ACTTCTGACC CACTGGGAAT GTGATGAAAG AAATAAAAGC TGAAATGAAT
     3661 CATTCTCTCT ACTATTATTC TGATATTTCA CATTCTTAAA ATAAAGTGGT GATCCTAACT
     3721 GACCTTAAGA CAGGGAATCT TTACTCGGAT TAAATGTCAG GAATTGTGAA AAAGTGAGTT
     3781 TAAATGTATT TGGCTAAGGT GTATGTAAAC TTCCGACTTC AACTGTAGGG GATCCTCTAG
     3841 AGTCGACCTG CAGGCATGCA AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA
     3901 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT
     3961 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC
     4021 AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
     4081 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
     4141 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
     4201 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA
     4261 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
     4321 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
     4381 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
     4441 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
     4501 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
     4561 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
     4621 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
     4681 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
     4741 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
     4801 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
     4861 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
     4921 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
     4981 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
     5041 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
     5101 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
     5161 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
     5221 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
     5281 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
     5341 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
     5401 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
     5461 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
     5521 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
     5581 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
     5641 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
     5701 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
     5761 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
     5821 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
     5881 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
     5941 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC
     6001 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT
     6061 TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG
     6121 GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG
     6181 CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC
     6241 TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC
     6301 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCCA TTCGCCATTC AGGCTGCGCA
     6361 ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG
     6421 GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA
     6481 AAACGACGGC CAGTG
//
