LOCUS       pCNDIiR2Ax              7618 bp ss-DNA     circular SYN 12-Feb-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     frag            join(1..>53,<624..>629,<631,<632..>957,<2043..7618)
                     /note="1 to 7861 of pCNIiR2Ax [Split]"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.439,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     frag            join(1..>53,<624..>629,<631,<632..>957,<2048..7618)
                     /note="1 to 7799 of pCNIiR_x [Split]"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.439,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     frag            join(1..>53,<624..>629,<631,<632..>957,<2054..7618)
                     /note="1 to 6990 of CaN_pCAGGsNRas_G12V_IRES [Split]"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.439,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     gene            54..623
                     /label="NRas G12V"
                     /mv_symbol_type=HollowArrow
                     /mv_fill_color="0.867,0.000,0.000,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="NRas G12V"
                     /mv_font_color="1.000,1.000,1.000,1.000"
                     /mv_label_position=Center
     misc_feature    640..1202
                     /label="IRES"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="0.200,1.000,1.000,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="IRES"
                     /mv_label_position=Center
                     /mv_visible=1
     enhancer        <958..1220
                     /label="IRES"
                     /note="/vntifkey=9 [Split]"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.439,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     frag            958..2042
                     /note="1981 to 3065 of Caggs-Nras-mV"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.439,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     frag            join(<958..>1226,<1227..2042)
                     /note="1 to 2642 of Untitled [Split]"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.439,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     frag            join(<958..>1226,<1227..2042)
                     /note="1 to 2642 of MSCV_IRES_dnMAML1-mVenus [Split]"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.439,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     frag            join(1225..>1226,<1227..1952)
                     /note="121 to 1061 of 417669901_DNB_IRES_406_B06 [Split]"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.439,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     CDS             <1227..1946
                     /note="mVenus"
                     /mv_symbol_type=HollowArrow
                     /mv_fill_color="0.200,1.000,0.200,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label_position=Center
     frag            <1227..1946
                     /note="1 to 933 of dnMAML-mVenus"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.439,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
     polyA_signal    2128..2355
                     /label="pA"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="1.000,0.658,0.000,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="pA"
                     /mv_font_color="1.000,1.000,1.000,1.000"
                     /mv_label_position=Center
                     /mv_visible=1
     misc_feature    2451..2755
                     /label="IR-DR(R)"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="0.800,0.658,1.000,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="IR-DR(R)"
                     /mv_font_color="1.000,1.000,1.000,1.000"
                     /mv_label_position=Center
                     /mv_visible=1
     gene            complement(3967..4626)
                     /label="AmpR"
                     /mv_symbol_type=HollowArrow
                     /mv_fill_color="0.467,0.512,0.467,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="AmpR"
                     /mv_font_color="1.000,1.000,1.000,1.000"
                     /mv_label_position=Center
     misc_feature    5421..5873
                     /label="IR-DR(L)"
                     /mv_symbol_type=HollowBox
                     /mv_fill_color="0.800,0.658,1.000,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="IR-DR(L)"
                     /mv_font_color="1.000,1.000,1.000,1.000"
                     /mv_label_position=Center
                     /mv_visible=1
     promoter        5894..7618
                     /label="CAG promoter"
                     /mv_symbol_type=HollowArrow
                     /mv_fill_color="1.000,0.439,0.400,1.000"
                     /mv_pen_color="0.000,0.000,0.000,1.000"
                     /mv_label="CAG promoter"
                     /mv_font_color="1.000,1.000,1.000,1.000"
                     /mv_label_position=Center
                     /mv_level=32767
BASE COUNT  1786 A  1943 C  2142 G  1747 T  0 OTHER
ORIGIN
        1 CTCGATACCG GGGCGCGTCC ACCATGGAGG AAGAGGAATA TATGCCTATG GAAATGACTG
       61 AGTACAAACT GGTGGTGGTT GGAGCAGTTG GTGTTGGGAA AAGCGCACTG ACAATCCAGC
      121 TAATCCAGAA CCACTTTGTA GATGAATATG ATCCCACCAT AGAGGCTTCT TACAGAAAAC
      181 AAGTGGTTAT AGATGGTGAA ACCTGTTTGT TGGACATACT GGATACAGCT GGACAAGAAG
      241 AGTACAGTGC CATGAGAGAC CAATACATGA GGACAGGCGA AGGCTTCCTC TGTGTATTTG
      301 CCATCAATAA TAGCAAGTCA TTTGCGGATA TTAACCTCTA CAGGGAACAG ATTAAGCGAG
      361 TAAAAGACTC GGATGATGTA CCTATGGTGC TAGTGGGAAA CAAGTGTGAT TTGCCAACAA
      421 GGACAGTTGA TACAAAACAA GCCCACGAAC TGGCCAAGAG TTACGGGATT CCATTCATTG
      481 AAACCTCAGC CAAGACCAGA CAGGGTGTTG AAGATGCTTT TTACACACTG GTAAGAGAAA
      541 TACGCCAGTA CCGAATGAAA AAACTCAACA GCAGTGATGA TGGGACTCAG GGTTGTATGG
      601 GATTGCCATG TGTGGTGATG TAAGCGGCCA ACGAATTCCG CCCCTCTCCC TCCCCCCCCC
      661 CTAACGTTAC TGGCCGAAGC CGCTTGGAAT AAGGCCGGTG TGCGTTTGTC TATATGTTAT
      721 TTTCCACCAT ATTGCCGTCT TTTGGCAATG TGAGGGCCCG GAAACCTGGC CCTGTCTTCT
      781 TGACGAGCAT TCCTAGGGGT CTTTCCCCTC TCGCCAAAGG AATGCAAGGT CTGTTGAATG
      841 TCGTGAAGGA AGCAGTTCCT CTGGAAGCTT CTTGAAGACA AACAACGTCT GTAGCGACCC
      901 TTTGCAGGCA GCGGAACCCC CCACCTGGCG ACAGGTGCCT CTGCGGCCAA AAGCCACGTG
      961 TATAAGATAC ACCTGCAAAG GCGGCACAAC CCCAGTGCCA CGTTGTGAGT TGGATAGTTG
     1021 TGGAAAGAGT CAAATGGCTC TCCTCAAGCG TATTCAACAA GGGGCTGAAG GATGCCCAGA
     1081 AGGTACCCCA TTGTATGGGA TCTGATCTGG GGCCTCGGTG CACATGCTTT ACATGTGTTT
     1141 AGTCGAGGTT AAAAAACGTC TAGGCCCCCC GAACCACGGG GACGTGGTTT TCCTTTGAAA
     1201 AACACGATGA TAATATGGCC ACAACCATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG
     1261 TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG
     1321 GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGCTGATC TGCACCACCG
     1381 GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GGGCTACGGC CTGCAGTGCT
     1441 TCGCCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG
     1501 GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG
     1561 AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA
     1621 AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT
     1681 ATATCACCGC CGACAAGCAG AAGAACGGCA TCAAGGCCAA CTTCAAGATC CGCCACAACA
     1741 TCGAGGACGG CGGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG
     1801 GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCTACCA GTCCAAGCTG AGCAAAGACC
     1861 CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC
     1921 TCGGCATGGA CGAGCTGTAC AAGTAAGTCG ACCTGCAGCC AAGCTTATCG ATAAAATAAA
     1981 AGATTTTATT TAGTCTCCAG AAAAAGGGGG GAATGAAAGA CCCCACCTGT AGGTTTGGCA
     2041 AGCTAGCCCC GGGAAAACTC AACAGCAGTG ATGATGGGAC TCAGGGTTGT ATGGGATTGC
     2101 CATGTGTGGT GATGTAAGGA TCAGCCTCGA CTGTGCCTTC TAGTTGCCAG CCATCTGTTG
     2161 TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC CACTCCCACT GTCCTTTCCT
     2221 AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG TCATTCTATT CTGGGGGGTG
     2281 GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG
     2341 CGGTGGGCTC TATGGCTTCT GAGGCGGAAA GAACCAGCCC ATCACAAAGC TCTGACCTCA
     2401 ATCCTATAGA AAGGAGGAAT GAGCCAAAAT TCACCCAACT TATTGTGGGA AGCTTGTGGA
     2461 AGGCTACTCG AAATGTTTGA CCCAAGTTAA ACAATTTAAA GGCAATGCTA CCAAATACTA
     2521 ATTGAGTGTA TGTTAACTTC TGACCCACTG GGAATGTGAT GAAAGAAATA AAAGCTGAAA
     2581 TGAATCATTC TCTCTACTAT TATTCTGATA TTTCACATTC TTAAAATAAA GTGGTGATCC
     2641 TAACTGACCT TAAGACAGGG AATCTTTACT CGGATTAAAT GTCAGGAATT GTGAAAAAGT
     2701 GAGTTTAAAT GTATTTGGCT AAGGTGTATG TAAACTTCCG ACTTCAACTG TAGGGGATCC
     2761 TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT
     2821 GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA
     2881 AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC
     2941 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG
     3001 AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT
     3061 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA
     3121 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG
     3181 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     3241 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     3301 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     3361 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     3421 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     3481 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     3541 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     3601 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT
     3661 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     3721 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     3781 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA
     3841 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT
     3901 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA
     3961 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC
     4021 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG
     4081 CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT
     4141 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT
     4201 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG
     4261 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC
     4321 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA
     4381 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC
     4441 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT
     4501 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG
     4561 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT
     4621 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG
     4681 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC
     4741 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC
     4801 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA
     4861 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG
     4921 GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT
     4981 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA
     5041 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC
     5101 GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG
     5161 CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA TGCGGTGTGA
     5221 AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCCATTCGC CATTCAGGCT
     5281 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA
     5341 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG
     5401 TTGTAAAACG ACGGCCAGTG AATTCGAGCT CGGTACCCGC AGTTGAAGTC GGAAGTTTAC
     5461 ATACACTTAA GTTGGAGTCA TTAAAACTCG TTTTTCAACT ACACCACAAA TTTCTTGTTA
     5521 ACAAACAATA GTTTTGGCAA GTCAGTTAGG ACATCTACTT TGTGCATGAC ACAAGTCATT
     5581 TTTCCAACAA TTGTTTACAG ACAGATTATT TCACTTATAA TTCACTGTAT CACAATTCCA
     5641 GTGGGTCAGA AGTTTACATA CACTAAGTTG ACTGTGCCTT TAAACAGCTT GGAAAATTCC
     5701 AGAAAATGAT GTCATGGCTT TAGAAGCTTC TGATAGACTA ATTGACATCA TTTGAGTCAA
     5761 TTGGAGGTGT ACCTGTGGAT GTATTTCAAG GCGATGTACG GGCCAGATAT ACGCGTTGAC
     5821 ATTGATTATT GACTAGTAAC GGCCGCCAGT GTGCTGGAAT TCTGCAGATA TCAAGCTTAT
     5881 CGATACCGTC GACCTCGACA TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG
     5941 TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG
     6001 CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA
     6061 GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG ACTATTTACG GTAAACTGCC
     6121 CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC
     6181 GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG
     6241 CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGGTC GAGGTGAGCC CCACGTTCTG
     6301 CTTCACTCTC CCCATCTCCC CCCCCTCCCC ACCCCCAATT TTGTATTTAT TTATTTTTTA
     6361 ATTATTTTGT GCAGCGATGG GGGCGGGGGG GGGGGGGGCG CGCGCCAGGC GGGGCGGGGC
     6421 GGGGCGAGGG GCGGGGCGGG GCGAGGCGGA GAGGTGCGGC GGCAGCCAAT CAGAGCGGCG
     6481 CGCTCCGAAA GTTTCCTTTT ATGGCGAGGC GGCGGCGGCG GCGGCCCTAT AAAAAGCGAA
     6541 GCGCGCGGCG GGCGGGAGTC GCTGCGTTGC CTTCGCCCCG TGCCCCGCTC CGCGCCGCCT
     6601 CGCGCCGCCC GCCCCGGCTC TGACTGACCG CGTTACTCCC ACAGGTGAGC GGGCGGGACG
     6661 GCCCTTCTCC TCCGGGCTGT AATTAGCGCT TGGTTTAATG ACGGCTCGTT TCTTTTCTGT
     6721 GGCTGCGTGA AAGCCTTAAA GGGCTCCGGG AGGGCCCTTT GTGCGGGGGG GAGCGGCTCG
     6781 GGGGGTGCGT GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCCCGCG CTGCCCGGCG
     6841 GCTGTGAGCG CTGCGGGCGC GGCGCGGGGC TTTGTGCGCT CCGCGTGTGC GCGAGGGGAG
     6901 CGCGGCCGGG GGCGGTGCCC CGCGGTGCGG GGGGGCTGCG AGGGGAACAA AGGCTGCGTG
     6961 CGGGGTGTGT GCGTGGGGGG GTGAGCAGGG GGTGTGGGCG CGGCGGTCGG GCTGTAACCC
     7021 CCCCCTGCAC CCCCCTCCCC GAGTTGCTGA GCACGGCCCG GCTTCGGGTG CGGGGCTCCG
     7081 TGCGGGGCGT GGCGCGGGGC TCGCCGTGCC GGGCGGGGGG TGGCGGCAGG TGGGGGTGCC
     7141 GGGCGGGGCG GGGCCGCCTC GGGCCGGGGA GGGCTCGGGG GAGGGGCGCG GCGGCCCCGG
     7201 AGCGCCGGCG GCTGTCGAGG CGCGGCGAGC CGCAGCCATT GCCTTTTATG GTAATCGTGC
     7261 GAGAGGGCGC AGGGACTTCC TTTGTCCCAA ATCTGGCGGA GCCGAAATCT GGGAGGCGCC
     7321 GCCGCACCCC CTCTAGCGGG CGCGGGCGAA GCGGTGCGGC GCCGGCAGGA AGGAAATGGG
     7381 CGGGGAGGGC CTTCGTGCGT CGCCGCGCCG CCGTCCCCTT CTCCATCTCC AGCCTCGGGG
     7441 CTGCCGCAGG GGGACGGCTG CCTTCGGGGG GGACGGGGCA GGGCGGGGTT CGGCTTCTGG
     7501 CGTGTGACCG GCGGCTCTAG AGCCTCTGCT AACCATGTTC ATGCCTTCTT CTTTTTCCTA
     7561 CAGCTCCTGG GCAACGTGCT GGTTGTTGTG CTGTCTCATC ATTTTGGCAA AGAATTCC
//
