LOCUS       Exported                7869 bp ds-DNA     circular SYN 06-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7869
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3624..3632
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   670..983
                     /label=5' ITR
     promoter        1010..2734
                     /label=CAGGS
     primer_bind     2716..2757
                     /label=2-F
     primer_bind     2716..2747
                     /label=7a-F
     misc_feature    2716..2734
                     /label=Overlap #1
     misc_feature    2735..2740
                     /label=Overlap #1
     misc_feature    2740
                     /label=BglII overhang
     misc_feature    2741..2744
                     /label=Kozak sequence
     CDS             2745..3623
                     /codon_start=1
                     /label=RPS2
                     /translation="MADDAGAAGGPGGPGGPGLGGRGGFRGGFGSGLRGRGRGRGRGRG
                     RGRGARGGKAEDKEWIPVTKLGRLVKDMKIKSLEEIYLFSLPIKESEIIDFFLGASLKD
                     EVLKIMPVQKQTRAGQRTRFKAFVAIGDYNGHVGLGVKCSKEVATAIRGAIILAKLSIV
                     PVRRGYWGNKIGKPHTVPCKVTGRCGSVLVRLIPAPRGTGIVSAPVPKKLLMMAGIDDC
                     YTSARGCTATLGNFAKATFDAISKTYSYLTPDLWKETVFTKSPYQEFTDHLVKTHTRVS
                     VQRTQAPAVATT"
     primer_bind     complement(3615..3643)
                     /label=2-R
     primer_bind     3624..3656
                     /label=GFP-F
     misc_feature    3624..3643
                     /label=Overlap #3
     CDS             3633..4868
                     /codon_start=1
                     /label=smGFP-V5
                     /translation="GKPIQNPLLGLDSTQQQGKPIPNPLLGLDSTQQQGKPIPNPLLGL
                     DSTGQGVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFICTTGKL
                     PVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDGTYKTRAEV
                     KFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFKIRHNV
                     EGGGKPIPNPLLGLDSTQQQGKPIPNPLLGLDSTGGQQGGGKPIPNPLLGLDSTQQQGK
                     PIPNPLLGLDSTGGDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGQGGKPIPNPLLGLDSTQQQGKPIPNPLLGLDSTQQQG
                     KPIPNPLLGLDST"
     CDS             3633..3674
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIQNPLLGLDST"
     CDS             3684..3725
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3735..3776
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3978..3986
                     /label=bright chromophore
     CDS             4308..4349
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4359..4400
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4419..4460
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4470..4511
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4725..4766
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4776..4817
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4827..4868
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4850..4891)
                     /label=GFP-R
     misc_feature    4872..4891
                     /label=Overlap #2
     misc_feature    4872..4875
                     /label=PmeI overhang
     polyA_site      4886..5165
                     /label=BGH pA
                     /label=BGPH pA
     repeat_region   5393..5634
                     /label=3' ITR
     misc_feature    5720..5736
                     /label=M13
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct ccccccccct
     1441 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
     1501 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
     1561 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     1621 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
     1681 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     1741 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     1801 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
     1861 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
     1921 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
     1981 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
     2041 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
     2101 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
     2161 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
     2221 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     2281 gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc
     2341 gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct
     2401 ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg
     2461 cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
     2521 tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc
     2581 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct
     2641 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
     2701 ctggttattg tgctgtctca tcattttggc aaagaattca caccatggcg gatgacgccg
     2761 gtgcagcggg agggcccgga ggacccgggg gcccaggatt aggtggccgc ggcggcttcc
     2821 gcggaggatt cggcagcggt cttaggggcc gtggtcgtgg ccgaggccgt ggccgtggtc
     2881 gaggccgcgg ggctcgtgga ggtaaagctg aagacaagga gtggatcccc gtcaccaagc
     2941 tgggccgtct ggttaaggac atgaagatca agtccttgga ggagatctac ctgttctccc
     3001 tgcccattaa ggagtctgag atcattgatt tcttcctggg tgcgtcccta aaggatgagg
     3061 ttctgaaaat catgccagtg cagaagcaga ctcgggctgg ccagcggacc aggttcaagg
     3121 ctttcgtcgc tattggggac tacaatggtc acgttggtct tggtgttaag tgctccaagg
     3181 aggttgccac tgccatccga ggggccatca tcttggccaa gctttccatc gtccctgtgc
     3241 ggagaggcta ctgggggaac aagattggca agccccacac tgttccatgc aaggtgacag
     3301 gccgctgtgg ctctgtgctg gtgcgtctca tccctgcccc cagaggcact ggcattgtct
     3361 ctgctcctgt gcccaagaag ctcctgatga tggccggtat agatgactgc tacacttcag
     3421 ccagaggctg cactgccacc ctgggcaact ttgctaaggc cacctttgat gccatctcca
     3481 agacttacag ctacctgact cccgacctct ggaaagagac tgtcttcacc aagtctcctt
     3541 atcaggaatt cacggatcat cttgtgaaaa cccacaccag agtctctgtt cagaggaccc
     3601 aggctccagc tgtggctacc acaggatcgg gcggtaagcc tatccaaaat ccactgttgg
     3661 ggctggatag cactcaacag caaggaaaac caatacccaa tccactactg ggccttgatt
     3721 caacgcagca gcagggtaaa ccgatcccga atcctcttct tggcctcgat agcaccggtc
     3781 aaggggttag taagggagaa gaactcttca caggtgttgt cccgatactt gtggaactcg
     3841 atggggacgt caatggccat aagtttagcg tgagaggtga gggcgagggc gacgccacga
     3901 acggtaagct gacacttaaa tttatatgca ctacaggaaa actccccgtg ccttggccca
     3961 ctctcgtgac aaccctcact tacggcgttc aatgtttttc aagatatcct gatcacatga
     4021 agcagcatga cttttttaaa tccgcgatgc ccgaaggata cgtccaagaa cgaactataa
     4081 gttttaagga cgatggtact tataaaactc gcgcagaggt aaagttcgaa ggggatactc
     4141 ttgttaacag gattgaactc aaaggaatcg atttcaaaga ggacgggaat attcttgggc
     4201 acaagctgga gtacaacttt aacagccaca acgtgtacat caccgctgac aagcaaaaaa
     4261 atggtatcaa ggcgaacttt aagatacggc acaatgtaga gggtggcgga aagcccatcc
     4321 ctaatccgct gctgggtctg gatagtacgc agcaacaagg taagccaatc cctaacccat
     4381 tgctcggact cgactctacc ggtgggcaac aaggtggtgg aaagcctatt ccaaacccgc
     4441 tgcttggact ggatagcacg caacagcagg ggaagccgat tcctaatcct ctcctggggt
     4501 tggactccac aggcggggat ggaagtgtgc agcttgcgga ccattatcag cagaacactc
     4561 caattgggga cggccccgta ttgcttcctg acaatcatta tctcagtact cagagtgttc
     4621 tctctaaaga ccccaatgag aagcgagatc acatggtgct ccttgagttt gtgaccgccg
     4681 caggaattac tcttggaatg gacgagttgt ataaggggca aggaggcaag ccaataccta
     4741 atcccctcct ggggctcgat tctacgcagc agcaaggtaa acccataccc aaccctcttt
     4801 tgggactgga ctcaactcag cagcaaggaa aacctatacc gaacccgttg ctgggtctcg
     4861 attctacttg aaaacgatga tgcatcgatg atctagagct cgctgatcag cctcgactgt
     4921 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     4981 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5041 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5101 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     5161 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5221 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5281 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5341 cctgtcgtgc cagcggatcc attcatgaat gaattcatgt cgacatacta gttaaaagtt
     5401 ttgttacttt atagaagaaa ttttgagttt ttgttttttt ttaataaata aataaacata
     5461 aataaattgt ttgttgaatt tattattagt atgtaagtgt aaatataata aaacttaata
     5521 tctattcaaa ttaataaata aacctcgata tacagaccga taaaacacat gcgtcaattt
     5581 tacgcatgat tatctttaac gtacgtcaca atatgattat ctttctaggg ttaatctagt
     5641 atacgcgtat gcggccgctt aattaatcca gcttttgttc cctttagtga gggttaattg
     5701 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     5761 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     5821 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     5881 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5941 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6001 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6061 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6121 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6181 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6241 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6301 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6361 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6421 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6481 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6541 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6601 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6661 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6721 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6781 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6841 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6901 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6961 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7021 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7081 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7141 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7201 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7261 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7321 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7381 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7441 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7501 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7561 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7621 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7681 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7741 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7801 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7861 aagtgccac
//
