LOCUS       Exported                7128 bp ds-DNA     circular SYN 06-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          2745..3128
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   670..983
                     /label=5' ITR
     promoter        1010..2734
                     /label=CAGGS
     primer_bind     2716..2759
                     /label=25-F
     misc_feature    2716..2734
                     /label=Overlap #1
     misc_feature    2735..2740
                     /label=Overlap #1
     misc_feature    2740
                     /label=BglII overhang
     misc_feature    2741..2744
                     /label=Kozak sequence
     CDS             2745..3119
                     /codon_start=1
                     /locus_tag="s25"
                     /label=RPS25
                     /label=s25
                     /translation="MPPKDDKKKKDAGKSAKKDKDPVNKSGGKAKKKKWSKGKVRDKLN
                     NLVLFDKATYDKLCKEVPNYKLITPAVVSERLKIRGSLARAALQELLSKGLIKLVSKHR
                     AQVIYTRNTKGGDAPAAGEDA"
     primer_bind     complement(3111..3139)
                     /label=25-R
     primer_bind     3120..3150
                     /label=Ruby-F
     misc_feature    3120..3139
                     /label=Overlap #3
     CDS             3129..4127
                     /codon_start=1
                     /label=smRuby2-HA
                     /translation="VYPYDVPDYAGYPYDVPDYAGYPYDVPDYAGGNSLIKENMRMKVV
                     LEGSVNGHQFKCTGEGEGNPYMGTQTMRIKVIEGGPLPFAFDILATSFMYGSRTFIKYP
                     KGIPDFFKQSFPEGFTWERVTRYEDGGVITVMQDTSLEDGCLVYHAQVRGVNFPSNGAV
                     MQKKTKGWEPNTEMMYPADGGLRGYTHMALKVDGGGYPYDVPDYAGGYPYDVPDYAGGG
                     GYPYDVPDYAGGHPYDVPDYAGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEE
                     SDNEMFVVQREHAVAKFAGLGGGYPYDVPDYAGYPYDVPDYAGYPYDVPDYA"
     CDS             3132..3158
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3162..3188
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3192..3218
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    3408..3416
                     /label=Bright chromophore
     CDS             3726..3752
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3759..3785
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3798..3824
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4041..4067
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4071..4097
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4101..4127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4110..4150)
                     /label=Ruby-R
     misc_feature    4131..4150
                     /label=Overlap #2
     misc_feature    4131..4134
                     /label=PmeI overhang
     polyA_site      4145..4424
                     /label=BGH pA
                     /label=BGPH pA
     repeat_region   4652..4893
                     /label=3' ITR
     misc_feature    4979..4995
                     /label=M13
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct ccccccccct
     1441 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
     1501 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
     1561 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     1621 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
     1681 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     1741 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     1801 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
     1861 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
     1921 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
     1981 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
     2041 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
     2101 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
     2161 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
     2221 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     2281 gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc
     2341 gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct
     2401 ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg
     2461 cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
     2521 tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc
     2581 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct
     2641 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
     2701 ctggttattg tgctgtctca tcattttggc aaagaattca caccatgcct cccaaagacg
     2761 acaagaagaa gaaagatgcc ggaaagtcgg ccaaaaagga taaagaccca gtaaataaat
     2821 ctggtggcaa ggccaagaag aagaagtggt ccaaaggcaa agttcgggac aagttgaaca
     2881 atcttgtcct gttcgacaaa gcgacatacg acaagctctg taaggaggtt ccgaactata
     2941 agcttattac tccagccgtg gtctctgaga gactgaagat tcgcggttcc ttggccaggg
     3001 cagcccttca ggagctcctt agtaaaggac ttatcaagct ggtttcaaag cacagagccc
     3061 aagtaattta caccagaaac acaaagggtg gggacgctcc agctgctggc gaagatgcag
     3121 gatcgggcgt ttatccctat gatgtgccgg attatgctgg atacccatac gatgtgcctg
     3181 attatgcagg atatccgtac gatgtgccag attacgcggg tggaaattct cttataaaag
     3241 aaaatatgag gatgaaggtg gttctggagg gctcagtcaa tgggcaccag ttcaaatgca
     3301 ctggagaagg agaaggtaat ccttatatgg gcacccaaac aatgaggatc aaggttattg
     3361 aaggtggacc gttgcccttt gctttcgata tcctggcgac gagttttatg tacggaagtc
     3421 ggacttttat aaagtatcca aaaggcattc ctgatttttt caagcaatcc tttccggaag
     3481 gctttacatg ggagagagtg acccggtacg aggatggcgg tgtgatcaca gtcatgcaag
     3541 atacgagctt ggaagacgga tgcttggtgt accacgccca agtacgagga gttaatttcc
     3601 catctaacgg cgccgtcatg caaaagaaaa ctaaaggatg ggagcccaac actgagatga
     3661 tgtatcctgc agatggcggt ttgagaggct atactcacat ggcgcttaaa gtagacggcg
     3721 gaggttatcc ttatgatgta cctgattatg ctggtggcta cccttacgat gtaccagact
     3781 acgccggtgg cggcggttat ccgtacgacg tgccagacta cgcaggaggg cacccttatg
     3841 acgtgcccga ctacgcggga ggacaccttt catgttcttt cgttaccact tatcgctcta
     3901 agaagaccgt gggcaatata aaaatgcccg gaatacatgc agtggaccat aggctcgaac
     3961 ggctcgaaga atctgataac gaaatgtttg ttgttcaacg cgaacacgcc gtggctaagt
     4021 tcgcaggtct cggaggcggc tatccttacg acgtccctga ctacgcagga tacccgtacg
     4081 atgtgcccga ttacgcaggt tacccgtatg atgtaccgga ctatgcgtaa aaacgatgat
     4141 gcatcgatga tctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     4201 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4261 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     4321 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     4381 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc tgcagcccaa gcttggcgta
     4441 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4501 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4561 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatcca
     4621 ttcatgaatg aattcatgtc gacatactag ttaaaagttt tgttacttta tagaagaaat
     4681 tttgagtttt tgtttttttt taataaataa ataaacataa ataaattgtt tgttgaattt
     4741 attattagta tgtaagtgta aatataataa aacttaatat ctattcaaat taataaataa
     4801 acctcgatat acagaccgat aaaacacatg cgtcaatttt acgcatgatt atctttaacg
     4861 tacgtcacaa tatgattatc tttctagggt taatctagta tacgcgtatg cggccgctta
     4921 attaatccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     4981 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     5041 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     5101 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     5161 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     5221 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5281 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5341 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5401 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5461 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5521 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5581 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5641 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5701 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5761 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5821 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5881 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5941 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6001 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6061 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6121 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6181 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6241 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6301 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6361 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6421 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6481 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6541 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6601 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6661 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6721 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6781 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6841 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6901 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6961 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7021 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7081 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
