LOCUS       Exported               10509 bp ds-DNA     circular SYN 06-MAR-2025
DEFINITION  Invitrogen T-Rex system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Mar 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10509
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        join(233..699,720..909)
                     /label=Pcmv/2xTetO2
                     /note="/vntifkey=29"
     misc_feature    941..943
                     /label=ATG
     misc_feature    995..1009
                     /label=New Feature
     CDS             1010..2737
                     /codon_start=1
                     /label=24xGCN4_v4
                     /translation="EELLSKNYHLENEVARLKKGSGSGEELLSKNYHLENEVARLKKGS
                     GSGEELLSKNYHLENEVARLKKGSGSGEELLSKNYHLENEVARLKKGSGSGEELLSKNY
                     HLENEVARLKKGSGSGEELLSKNYHLENEVARLKKGSGSGEELLSKNYHLENEVARLKK
                     GSGSGEELLSKNYHLENEVARLKKGSGSGEELLSKNYHLENEVARLKKGSGSGEELLSK
                     NYHLENEVARLKKGSGSGEELLSKNYHLENEVARLKKGSGSGEELLSKNYHLENEVARL
                     KKGSGSGEELLSKNYHLENEVARLKKGSGSGEELLSKNYHLENEVARLKKGSGSGEELL
                     SKNYHLENEVARLKKGSGSGEELLSKNYHLENEVARLKKGSGSGEELLSKNYHLENEVA
                     RLKKGSGSGEELLSKNYHLENEVARLKKGSGSGEELLSKNYHLENEVARLKKGSGSGEE
                     LLSKNYHLENEVARLKKGSGSGEELLSKNYHLENEVARLKKGSGSGEELLSKNYHLENE
                     VARLKKGSGSGEELLSKDYHLENEVARLKKGSGSGEELLSKNYHLENEVARLKKGSGSG
                     "
     primer_bind     2732..2764
                     /label=Pcc-F
     misc_feature    2732..2748
                     /label=Overlap #1
     misc_feature    2748
                     /label=AgeI scar
     misc_feature    2749
                     /label=Spacer
     CDS             2750..4918
                     /codon_start=1
                     /label=PCC
                     /translation="AGQWVRTVALLAARRHWRRSSQQQLLGTLKHAPVYSYQCLVVSRS
                     LSSVEYEPKEKTFDKILIANRGEIACRVIKTCKKMGIKTVAIHSDVDASSVHVKMADEA
                     VCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFAKRLAAEDVTFIGPDT
                     HAIQAMGDKIESKLLAKRAKVNTIPGFDGVVKDADEAVRIAREIGYPVMIKASAGGGGK
                     GMRIAWDDEETRDGFRFSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLN
                     ERECSIQRRNQKVVEEAPSIFLDPETRQAMGEQAVALAKAVKYSSAGTVEFLVDSQKNF
                     YFLEMNTRLQVEHPVTECITGLDLVQEMILVAKGYPLRHKQEDIPISGWAVECRVYAED
                     PYKSFGLPSIGRLSQYQEPIHLPGVRVDSGIQPGSDISIYYDPMISKLVTYGSDRAEAL
                     KRMEDALDNYVIRGVTHNIPLLREVIINTRFVKGDISTKFLSDVYPDGFKGHTLTLSER
                     NQLLAIASSVFVASQLRAQRFQEHSRVPVIRPDVAKWELSVKLHDEDHTVVASNNGPAF
                     TVEVDGSKLNVTSTWNLASPLLSVNVDGTQRTVQCLSREAGGNMSIQFLGTVYKVHILT
                     KLAAELNKFMLEKVPKDTSSTLCSPMPGVVVAVSVKPGDMVAEGQEICVIEAMKMQNSM
                     TAGKMGKVKLVHCKAGDTVGEGDLLVELE"
     misc_feature    3797..3816
                     /label=Overlap #3
     primer_bind     complement(4898..4940)
                     /label=Pcc-R
     misc_feature    4919..4940
                     /label=Overlap #2
     misc_feature    4919..4921
                     /label=EcoRV scar
     misc_feature    4922..4924
                     /label=STOP
     misc_feature    4972..6427
                     /label=24xPP7 sites
     misc_feature    6523..6525
                     /label=BGH polyA
                     /label=BGH polyA(1)
     polyA_site      6526..6750
                     /label=BGHpA
                     /note="/vntifkey=26"
     misc_feature    6526..6750
                     /label=BGH polyA
     misc_feature    7235..7574
                     /label=SV40 early promoter and origin
     misc_feature    7615..7680
                     /label=EM-7 promoter - bacteria
     misc_feature    7681..8055
                     /label=Zeocin
     misc_feature    8186..8316
                     /label=SV40 poly adenylation
     CDS             complement(9513..10373)
                     /label=AmpR
                     /note="/vntifkey=4"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcag ctcagctttc tagaccgcgc
      721 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tccctatcag
      781 tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca
      841 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      901 cagcctccgg actctagcgt ttaaacttaa gcttgccacc atgggcgcca tcatacacgc
      961 ggccgcaatc atcggtccgg gtggatctgg aggtggaggt tctggaggag aagaactttt
     1021 gagcaagaat tatcatcttg agaacgaagt ggctcgtctt aagaaaggtt ctggcagtgg
     1081 agaagaactg ctttcaaaga attaccacct ggaaaatgag gtagctagac tgaaaaaggg
     1141 gagcggaagt ggggaggagt tgctgagcaa aaattatcat ttggagaacg aagtagcacg
     1201 actaaagaaa gggtccggat cgggtgagga gttactctcg aaaaattatc atctcgaaaa
     1261 cgaagtggct cggctaaaaa agggcagtgg ttctggagaa gagctattat ctaaaaacta
     1321 ccacctcgaa aatgaggtgg cacgcttaaa aaagggaagt ggcagtggtg aagagctact
     1381 atccaagaat tatcatcttg agaacgaggt agcgcgtttg aagaagggtt ccggctcagg
     1441 agaggaactg ctctcgaaga actatcatct tgaaaatgag gtcgctcgat taaaaaaggg
     1501 atcgggcagt ggtgaggaac tactttcaaa gaattaccac ctcgaaaacg aagtagctcg
     1561 attaaagaaa ggttcagggt cgggtgaaga attactgagt aaaaattatc atctggaaaa
     1621 tgaggtagcg agactaaaaa aggggagtgg ttctggcgaa gagttgctat cgaaaaatta
     1681 tcatcttgag aacgaagttg ctaggctcaa aaagggctca ggctcaggcg aggagttgct
     1741 ctcgaaaaac taccacttgg aaaatgaggt cgcgaggttg aaaaagggga gcgggtcggg
     1801 cgaggagtta ttgagcaaaa actatcattt agagaacgaa gtcgcgcgct taaagaaagg
     1861 ctcgggctcg ggcgaagaac tcttatcgaa gaactaccac ctcgaaaatg aggtcgccag
     1921 gttgaaaaag ggcagtggca gcggggagga actcttgagc aagaactacc acttggagaa
     1981 tgaggtcgcg agattgaaga aagggtcggg gagcggcgag gaattgctca gcaagaatta
     2041 tcatttggag aacgaagtcg ccaggctcaa gaaaggctcg gggtcggggg aggaattgtt
     2101 gagtaaaaac taccacttgg aaaatgaagt cgccaggctc aaaaaaggga gtgggagcgg
     2161 cgaagagtta ttgagcaaaa attaccactt ggagaacgaa gtggcaaggc tcaagaaagg
     2221 gagcggcagc ggggaggagc tcttatcgaa gaactaccac ttagagaatg aagtcgcccg
     2281 cttgaagaaa ggctcgggga gcggggaaga gctcttgagc aagaactacc acttggaaaa
     2341 tgaggtggcg cgcttgaaga aagggagcgg gagcggggaa gagttactat ctaagaatta
     2401 tcatctcgag aacgaggtgg ctcgactaaa gaagggctcc ggcagtgggg aggaactcct
     2461 gtcgaagaac tatcatcttg aaaatgaggt tgcaagactt aaaaaggggt ccggatcagg
     2521 tgaggaacta ctcagtaaga attaccacct ggaaaacgaa gttgcacgtt tgaagaaagg
     2581 atcaggatca ggcgaagaac tgctctcaaa agattatcat ttggaaaatg aggttgcacg
     2641 tttaaaaaag ggaagtggca gtggtgagga acttctgtcg aaaaattatc atctcgagaa
     2701 tgaagtagcc cgacttaaaa agggttctgg ctcgggtcag cggccgcagg cggggcagtg
     2761 ggtcaggacc gtggcgctgt tggcggccag gcggcattgg cggcggtcct cgcagcagca
     2821 attgctgggg acgctgaagc atgctccagt ctattcatac caatgcctag tggtgtccag
     2881 aagtctcagt tctgtggaat atgagcctaa agaaaagact tttgataaaa ttctcattgc
     2941 taacagagga gaaattgcct gtagggttat taaaacttgc aagaagatgg gcatcaagac
     3001 agttgccatt cacagtgatg ttgatgccag ttctgttcac gtgaaaatgg cggatgaggc
     3061 tgtctgtgtt ggcccagctc ccaccagtaa aagctacctc aacatggatg ccatcatgga
     3121 agccattaag aaaaccaggg cccaagctgt acacccaggg tatggattcc tgtcagaaaa
     3181 caaagagttt gcaaagcgtc tggcagcaga agatgtcact ttcattggac ctgatactca
     3241 tgctattcaa gccatgggtg acaagataga aagcaaacta ttagccaaga gagcaaaggt
     3301 caacacaatc cctggttttg atggggtagt aaaggatgca gatgaagctg tcagaattgc
     3361 aagggaaatt ggctaccctg tgatgatcaa ggcctcagca ggcggtggtg ggaaaggcat
     3421 gcgcatcgcc tgggatgacg aagagaccag ggatggcttt agattttcat cccaggaagc
     3481 tgcttctagt tttggtgatg atagactact aatagaaaaa tttatcgata accctcgtca
     3541 tatagaaatc caggttttag gggataaaca tggcaatgct ctgtggctca atgagaggga
     3601 gtgctcgatc cagagaagga accagaaggt ggtagaggag gcgccaagca tttttctgga
     3661 tcctgaaact cgccaagcaa tgggagagca ggccgtggct ttggctaaag ccgtgaagta
     3721 ttcttctgct ggaactgtgg aatttcttgt ggactcccag aagaattttt acttcttgga
     3781 gatgaataca agactacagg tcgaacatcc tgtcacagag tgcattactg gcctggactt
     3841 agtccaagaa atgatccttg ttgctaaggg ttacccactc aggcacaagc aagaggatat
     3901 tcccatcagt ggctgggcag ttgaatgtcg ggtttatgct gaggacccct acaagtcttt
     3961 cggtttaccg tctattggga ggctgtccca gtaccaagag ccgatacatc tacctggtgt
     4021 ccgagttgac agtggcatcc aaccaggaag tgacatcagc atctattatg atcctatgat
     4081 ttcaaagcta gtcacatatg ggtctgacag agcagaagcc ctgaagagga tggaagacgc
     4141 actggacaat tatgtgatcc ggggtgttac acacaacatc ccattgctcc gggaggtgat
     4201 aatcaacaca cgttttgtga aaggagacat cagcactaag tttctctctg atgtgtatcc
     4261 tgatggcttc aaagggcaca cgttaacact gagtgagaga aaccagttat tggccattgc
     4321 atcatctgta tttgtggcat cccagctacg agctcagcgc ttccaagaac attcaagagt
     4381 accagttatt aggcctgatg tggctaagtg ggagctctcg gtaaagttac atgatgaaga
     4441 tcatactgtc gtggcatcta acaatgggcc ggcatttacc gtggaagttg atggctcgaa
     4501 actaaatgtg accagtacgt ggaacctggc gtcaccctta ttgtctgtca acgttgatgg
     4561 cacgcagagg actgtgcagt gtctttctcg ggaagcaggt ggaaacatga gcatccagtt
     4621 tcttggcaca gtgtacaaag tgcacatttt aaccaagctt gctgcagagc tgaacaaatt
     4681 catgcttgaa aaagtgccca aggacaccag cagcactctg tgctccccga tgcctggagt
     4741 ggtggtggcc gtttctgtca agcctggaga catggtagca gaaggtcagg aaatctgtgt
     4801 gattgaagct atgaaaatgc agaacagtat gacagctggg aaaatgggca aggtgaaatt
     4861 ggtgcactgc aaagctggag acacagttgg tgaaggagac ctgcttgtgg agctggaaat
     4921 ctaacgacac gattcgaaga tcgcatcgct agcccggttg tacaggtacc ggatcctaag
     4981 gtacctaatt gcctagaaag gagcagacga tatggcgtcg ctccctgcag gtcgactcta
     5041 gaaaccagca gagcatatgg gctcgctggc tgcagtattc ccgggttcat tagatcctaa
     5101 ggtacctaat tgcctagaaa ggagcagacg atatggcgtc gctccctgca ggtcgactct
     5161 agaaaccagc agagcatatg ggctcgctgg ctgcagtatt cccgggttca ttagatccta
     5221 aggtacctaa ttgcctagaa aggagcagac gatatggcgt cgctccctgc aggtcgactc
     5281 tagaaaccag cagagcatat gggctcgctg gctgcagtat tcccgggttc attagatcct
     5341 aaggtaccta attgcctaga aaggagcaga cgatatggcg tcgctccctg caggtcgact
     5401 ctagaaacca gcagagcata tgggctcgct ggctgcagta ttcccgggtt cattagatcc
     5461 taaggtacct aattgcctag aaaggagcag acgatatggc gtcgctccct gcaggtcgac
     5521 tctagaaacc agcagagcat atgggctcgc tggctgcagt attcccgggt tcattagatc
     5581 ctaaggtacc taattgccta gaaaggagca gacgatatgg cgtcgctccc tgcaggtcga
     5641 ctctagaaac cagcagagca tatgggctcg ctggctgcag tattcccggg ttcattagat
     5701 cctaaggtac ctaattgcct agaaaggagc agacgatatg gcgtcgctcc ctgcaggtcg
     5761 actctagaaa ccagcagagc atatgggctc gctggctgca gtattcccgg gttcattaga
     5821 tcctaaggta cctaattgcc tagaaaggag cagacgatat ggcgtcgctc cctgcaggtc
     5881 gactctagaa accagcagag catatgggct cgctggctgc agtattcccg ggttcattag
     5941 atcctaaggt acctaattgc ctagaaagga gcagacgata tggcgtcgct ccctgcaggt
     6001 cgactctaga aaccagcaga gcatatgggc tcgctggctg cagtattccc gggttcatta
     6061 gatcctaagg tacctaattg cctagaaagg agcagacgat atggcgtcgc tccctgcagg
     6121 tcgactctag aaaccagcag agcatatggg ctcgctggct gcagtattcc cgggttcatt
     6181 agatcctaag gtacctaatt gcctagaaag gagcagacga tatggcgtcg ctccctgcag
     6241 gtcgactcta gaaaccagca gagcatatgg gctcgctggc tgcagtattc ccgggttcat
     6301 tagatcctaa ggtacctaat tgcctagaaa ggagcagacg atatggcgtc gctccctgca
     6361 ggtcgactct agaaaccagc agagcatatg ggctcgctgg ctgcagtatt cccgggttca
     6421 ttagatctcg cgaagggcga attcacgagg cgcgcccgga tcgcgatcgc aatcttaatt
     6481 aactcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     6541 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6601 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     6661 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     6721 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     6781 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6841 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6901 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6961 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7021 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7081 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7141 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7201 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     7261 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7321 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7381 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7441 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7501 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7561 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     7621 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     7681 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     7741 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     7801 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     7861 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     7921 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     7981 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     8041 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     8101 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     8161 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     8221 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8281 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     8341 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8401 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8461 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8521 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8581 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8641 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8701 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8761 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8821 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8881 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8941 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9001 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9061 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9121 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9181 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9241 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt
     9301 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9361 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9421 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9481 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9541 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9601 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9661 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9721 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9781 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9841 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9901 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9961 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10021 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10081 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10141 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10201 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    10261 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10321 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10381 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10441 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10501 cctgacgtc
//
