LOCUS       Exported                8663 bp ds-DNA     circular SYN 03-JUL-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    tagRFP-GPHN.FingR-HaloTag-updated
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Don Arnold
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 7, 2025 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    24..35
                     /note="CCR5ZFL BS"
     enhancer        36..394
                     /note="CMV Enhancer"
     promoter        395..1658
                     /note="beta-actin promoter"
     intron          1661..1705
     misc_feature    1766..1777
                     /note="CCR5ZFL BS"
     misc_feature    1784..2122
                     /note="CCR5 Left-handed ZF"
     CDS             2129..2890
                     /codon_start=1
                     /note="tagRFP-4GGGS"
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLNGGGSGGGSGGGSGGGSPW"
     CDS             2891..3158
                     /codon_start=1
                     /note="GPHN.FingR"
                     /translation="LEVKEASPTSIQISWGKYKVMVRYYRITYGETGGNSPVQEFTVPG
                     SKSTATISSLKPGVDYTITVYAVTIDHWNYQDPIPISINYRTGS"
     misc_feature    3164..3211
                     /note="4GGGS"
     misc_feature    3218..3640
                     /note="TPR3"
     CDS             3665..4546
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /note="HaloTag(R)"
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     intron          4582..4722
     enhancer        4725..5326
                     /note="WPRE"
     polyA_signal    5348..5488
                     /note="BGH PolyA"
     polyA_signal    5606..6332
                     /note="Beta-globin PolyA"
     rep_origin      complement(6334..6670)
                     /direction=LEFT
                     /note="SV40 rep ori"
     misc_feature    complement(7675..8532)
                     /note="AmpR"
ORIGIN
        1 gtcgacattg attattgact agagtcatcc tcatcgtcga cattgattat tgactagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggg
      421 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
      481 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      541 cgcgcgccag gcggggcggg gcgggggcga gggcggggcg gggcgaggcg gagaggtgcg
      601 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      661 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc
      721 cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
      781 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
      841 tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct
      901 ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
      961 gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
     1021 ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg
     1081 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
     1141 cgcggcggtc gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc
     1201 cggcttcggg tgcggggctc cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg
     1261 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     1321 gggaggggcg cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
     1381 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctggcg
     1441 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcgggcg aagcggtgcg
     1501 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
     1561 ttctccatct ccagcctcgg ggctgccgca gggggacggc tgccttcggg ggggacgggg
     1621 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     1681 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca
     1741 tcattttggc aaagaattcg gtaccgtcat cctcatcatt atgttccagt gccggatctg
     1801 catgcggaac ttcagcgacc ggtccaacct gagcaggcac atcagaaccc acaccggaga
     1861 aaagcccttc gcctgcgaca tttgcggccg gaagttcgcc atcagcagca acctgaacag
     1921 ccacaccaag atccacactg gcagccagaa acctttccag tgcagaattt gtatgagaaa
     1981 ctttagcaga agcgacaacc tggccagaca catccggaca catactggtg aaaaaccttt
     2041 tgcctgtgat atctgtggca gaaagtttgc cacctccggc aatctgaccc ggcacacaaa
     2101 gattcacctg cggggcagcc agggatccgt gtctaagggc gaagagctga ttaaggagaa
     2161 catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc
     2221 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg
     2281 cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcagaac
     2341 cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc ctgagggctt
     2401 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
     2461 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcccatc
     2521 caacggccct gtgatgcaga agaaaacact cggctgggag gccaacaccg agatgctgta
     2581 ccccgctgac ggcggcctgg aaggcagaag cgacatggcc ctgaagctcg tgggcggggg
     2641 ccacctgatc tgcaacttca agaccacata cagatccaag aaacccgcta agaacctcaa
     2701 gatgcccggc gtctactatg tggaccacag actggaaaga atcaaggagg ccgacaaaga
     2761 gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact
     2821 ggggcacaaa cttaatggtg gaggttctgg aggcggttca ggtggaggtt ctggaggcgg
     2881 ttctccatgg ctcgaagtca aggaagcatc accaaccagc atccagatca gctggggcaa
     2941 gtacaaggtc atggttcgct actaccgcat cacctacggt gaaactggtg gcaatagccc
     3001 tgtccaggaa ttcaccgtgc ctggcagcaa gtccactgct accatcagca gcctgaaacc
     3061 tggtgtcgac tataccatca cggtgtacgc cgtcacgatc gaccactgga actaccagga
     3121 cccgatcccg atctccatca actaccgcac cggttcagct agcggtggag gttctggtgg
     3181 aggttctgga ggtggatcag gaggaggttc gagatctaag ctctcaggtg gcggagggag
     3241 cggcggaggt ggctctggag gtggtggctc agctgaagca tggtacaatc tgggaaacgc
     3301 ttattacaag cagggcgact atcaaaaagc cattgagtat taccaaaagg ctcttgagct
     3361 agaccctaat aacgccgaag cgtggtataa cctaggcaac gcatattaca agcagggaga
     3421 ctaccaaaag gcaatcgaat attaccagaa ggcactcgaa ctggacccaa ataacgcaga
     3481 ggcttggtat aatctgggaa acgcctacta taaacagggt gactaccaaa aggcgataga
     3541 ggactatcag aaggccttgg agctcgatcc gaacaatctg caacgtagtg ctggtggcgg
     3601 agggtctggt ggaggaggct caggaggagg tggcgcatca atcgatgcat taagcgccac
     3661 gcgtgaaatc ggtactggct ttccattcga cccccattat gtggaagtcc tgggcgagcg
     3721 catgcactac gtcgatgttg gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa
     3781 cccgacctcc tcctacgtgt ggcgcaacat catcccgcat gttgcaccga cccatcgctg
     3841 cattgctcca gacctgatcg gtatgggcaa atccgacaaa ccagacctgg gttatttctt
     3901 cgacgaccac gtccgcttca tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt
     3961 cctggtcatt cacgactggg gctccgctct gggtttccac tgggccaagc gcaatccaga
     4021 gcgcgtcaaa ggtattgcat ttatggagtt catccgccct atcccgacct gggacgaatg
     4081 gccagaattt gcccgcgaga ccttccaggc cttccgcacc accgacgtcg gccgcaagct
     4141 gatcatcgat cagaacgttt ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct
     4201 gactgaagtc gagatggacc attaccgcga gccgttcctg aatcctgttg accgcgagcc
     4261 actgtggcgc ttcccaaacg agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct
     4321 ggtcgaagaa tacatggact ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg
     4381 caccccaggc gttctgatcc caccggccga agccgctcgc ctggccaaaa gcctgcctaa
     4441 ctgcaaggct gtggacatcg gcccgggtct gaatctgctg caagaagaca acccggacct
     4501 gatcggcagc gagatcgcgc gctggctgtc tactctggag atttcctaac aattgagtaa
     4561 agcggccccg gactcgaggc cgcaggtaag tatcaaggtt acaagacagg tttaaggaga
     4621 ccacatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta
     4681 ttggtcttac tgacatccac tttgcctttc tctccacagg tgtcgacaat caacctctgg
     4741 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4801 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     4861 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     4921 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     4981 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5041 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5101 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     5161 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5221 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5281 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtacgatc
     5341 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     5401 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     5461 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     5521 ggaggattgg gaagacaata gcaggccagc ttttgttccc tttagggtta attgcgcgct
     5581 tggcgctaat catggtcata gctgtttcca ctcctcaggt gcaggctgcc tatcagaagg
     5641 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatcgt tttccctctg
     5701 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     5761 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     5821 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     5881 atgccatatg ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa
     5941 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     6001 ttttatattt tgtttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     6061 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     6121 ttatgaagat ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc
     6181 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6241 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6301 cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc
     6361 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     6421 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     6481 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta
     6541 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6601 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6661 atcatgtctg gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6721 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6781 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6841 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6901 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6961 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7021 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7081 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     7141 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7201 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7261 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7321 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     7381 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7441 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7501 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7561 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7621 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7681 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7741 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7801 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7861 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7921 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7981 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8041 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8101 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8161 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8221 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8281 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8341 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8401 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8461 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8521 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8581 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8641 atttccccga aaagtgccac ctg
//
