LOCUS       EK0007-PB-4XnrUAS-H2B-B 8017 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
KEYWORDS    "accession:urn.local...1367881019035" "marker:5'TR, 3'TR, 6, 730 R,
            Xenopus globin 5'-UTR"
FEATURES             Location/Qualifiers
     primer          complement(620..638)
                     /label="FrtF"
                     /note="sequence: agggttccgatttagtgct"
     primer          756..771
                     /label="FlpInR"
                     /note="sequence: cgcgtaaccaccacac"
     primer          926..942
                     /label="M13-40FOR Genewiz"
                     /note="sequence: GTTTTCCCAGTCACGAC"
     primer          946..961
                     /label="M13F Genewiz"
                     /note="sequence: GTAAAACGACGGCCAG"
     primer_bind     1030..1049
                     /label="BII(R)"
     repeat_region   1092..1104
                     /label="5'TR"
     repeat_region   1105..1135
                     /label="5'TR(1)"
     misc_feature    1105..1135
                     /label="31bp spacer"
     repeat_region   1136..1137
                     /label="5'TR"
     repeat_region   1139..1154
                     /label="5'TR"
     enhancer        1330..1561
                     /label="core insul"
     primer_bind     1575..1575
                     /label="MluI-PGK-GF"
     misc_feature    1575..1575
                     /label="Gibson overlap"
     misc_feature    1575..1575
                     /label="Added MluI site"
     primer          1577..1653
                     /label="EKonrUAS.1"
                     /note="sequence: aattggcaaaaagcttaggcctccaaggCGGTGGCTTCTAATCCGTGAGTCCTAGCGGGTGACAGCCCTCCGTCTTC"
     primer          complement(1581..1658)
                     /label="EKonrUAS.3"
                     /note="sequence: CCTGTGAAGACGGAGGGCTGTCACCCGCTAGGACTCACGGATTAGAAGCCACCGccttggaggcctaagctttttgcc"
     misc_feature    1593..1859
                     /label="UAS"
     misc_feature    1605..1621
                     /label="UAS"
     misc_feature    1605..1702
                     /label="4x non-repetitve UAS from Akitake et al. Developmental Biology 2011"
     misc_feature    1632..1648
                     /label="UAS"
     primer          1654..1715
                     /label="EKonrUAS.2"
                     /note="sequence: ACAGGCGGAGGAGAGTCTTCCGTAGGGTTCCTCGGAGTACTGTCCTCCGgcaagggtcgact"
     misc_feature    1659..1675
                     /label="UAS"
     primer          complement(1659..1719)
                     /label="EKonrUAS.4"
                     /note="sequence: ctagagtcgacccttgcCGGAGGACAGTACTCCGAGGAACCCTACGGAAGACTCTCCTCCG"
     misc_feature    1686..1702
                     /label="UAS"
     5'UTR           1807..1849
                     /label="Xenopus globin 5'-UTR"
     misc_feature    1885..1885
                     /label="added SpeI site"
     misc_feature    1886..1891
                     /label="Added XhoI site"
     misc_feature    1892..1897
                     /label="Kozak"
     CDS             1898..2275
                     /label="H2B"
     CDS             1898..3001
                     /label="Translation 1898-3001"
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAITKYTSAKDPRVPVVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLN"
     CDS             2276..2293
                     /label="linker"
     misc_feature    2294..3001
                     /label="mtagBFP2"
     primer          2545..2562
                     /label="mTagBFP2.1"
                     /note="sequence: gcagtccttccctgaggg"
     CDS             3002..3004
                     /label="Translation 3002-3004"
                     /translation="*"
     misc_feature    3010..3032
                     /label="MCS"
     primer          complement(3029..3046)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
     polyA_signal    3033..3242
                     /label="BGH polyadenylation sequence"
     primer          complement(3108..3127)
                     /label="BghR1"
                     /note="sequence: attaggaaaggacagtggga"
     primer          3219..3237
                     /label="bghF"
                     /note="sequence: AGACAATAGCAGGCATGCT"
     misc_feature    3242..3243
                     /label="GATC"
     misc_feature    3244..3251
                     /label="NotI"
     primer_bind     3252..3280
                     /label="5, 299 F"
     misc_feature    3286..3286
                     /label="ES003-2 C->A"
     misc_feature    3293..3601
                     /label="pSV40"
     misc_feature    3658..4683
                     /label="Hygro"
     primer_bind     3849..3865
                     /label="2, 563 F"
     primer          3889..3910
                     /label="EKo152.1"
                     /note="sequence: cttgacattggggaattcagcg"
     primer_bind     4421..4437
                     /label="3, 135 F"
     primer          4609..4627
                     /label="Hyg_F"
                     /note="sequence: ACCGATGGCTGTGTAGAAG"
     misc_feature    complement(4663..4683)
                     /label="6, 730 R"
     misc_feature    4684..4689
                     /label="SalI"
     misc_feature    4690..4794
                     /label="WPRE"
     misc_feature    4800..4803
                     /label="SV40 pA(1)"
     misc_feature    4804..4807
                     /label="SV40 pA(1)"
     misc_feature    4805..4935
                     /label="SV40 pA"
     misc_feature    4808..4808
                     /label="SV40 pA(1)"
     misc_feature    4809..4809
                     /label="SV40 pA(1)"
     misc_feature    4810..4935
                     /label="SV40 pA(1)"
     primer          complement(4818..4843)
                     /label="SV40PA_R"
                     /note="sequence: GCTTTATTTGTAACCATTATAAGCTG"
     primer          complement(4842..4861)
                     /label="SV40pA-R Genewiz"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
     enhancer        complement(5184..5414)
                     /label="core insul(1) Geneious name: core insul(1)"
     repeat_region   5689..5723
                     /label="3'TR"
     misc_feature    5753..5753
                     /label="ES003-3 C->A"
     primer          complement(6214..6230)
                     /label="M13R Genewiz"
                     /note="sequence: CAGGAAACAGCTATGAC"
     primer          complement(6228..6247)
                     /label="M13-48REV Genewiz"
                     /note="sequence: CGGATAACAATTTCACACAG"
     primer_bind     complement(6242..6261)
                     /label="pLac(F)"
     primer          6506..6525
                     /label="pUCori5F"
                     /note="sequence: AAGGCGGTAATACGGTTATC"
     primer          complement(6677..6696)
                     /label="pUCoriR"
                     /note="sequence: tatagtcctgtcgggtttcg"
     misc_feature    7057..7057
                     /label="g->a"
     misc_feature    complement(7375..218)
                     /label="Amp"
     primer          complement(7500..7518)
                     /label="EKo152.2"
                     /note="sequence: tctcgcggtatcattgcag"
ORIGIN
        1 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
       61 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
      121 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
      181 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
      241 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
      301 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
      361 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
      421 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
      481 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
      541 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
      601 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
      661 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
      721 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
      781 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
      841 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      901 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      961 gtgagcgcgc ctcgttcatt cacgtttttg aacccgtgga ggacgggcag actcgcggtg
     1021 caaatgtgtt ttacagcgtg atggagcaga tgaagatgct cgacacgctg cagaacacgc
     1081 agctagatta accctagaaa gataatcata ttgtgacgta cgttaaagat aatcatgtgt
     1141 aaaattgacg catgtgtttt atcggtctgt atatcgaggt ttatttatta atttgaatag
     1201 atattaagtt ttattatatt tacacttaca tactaataat aaattcaaca aacaatttat
     1261 ttatgtttat ttatttatta aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa
     1321 aacttttatg agggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc
     1381 cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat
     1441 cccagagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt
     1501 tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa
     1561 aggcctccac ggccacaatt ggcaaaaagc ttaggcctcc aaggCGGTGG CTTCTAATCC
     1621 GTGAGTCCTA GCGGGTGACA GCCCTCCGTC TTCACAGGCG GAGGAGAGTC TTCCGTAGGG
     1681 TTCCTCGGAG TACTGTCCTC CGgcaagggt cgactctaga gggtatataa tggatcccat
     1741 cgcgtctcag cctcactttg agctcctcca cacgaattcc ccgataccgt cgattcaagg
     1801 agcttgcttg ttctttttgc agaagctcag aataaacgct caactttggc agatctacca
     1861 agcttggtac cgagctcgga tccaactcga ggccaccatg ccagagccag cgaagtctgc
     1921 tcccgccccg aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa
     1981 gaagcgcaag cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca
     2041 ggtccaccct gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa
     2101 cgacattttc gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc
     2161 gaccatcacc tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc
     2221 caagcacgcc gtgtccgagg gtactaaggc catcaccaag tacaccagcg ctaaggatcc
     2281 ccgggtaccg gtcgtgtcta agggcgaaga gctgattaag gagaacatgc acatgaagct
     2341 gtacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa
     2401 gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt
     2461 cgccttcgac atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac
     2521 ccagggcatc cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt
     2581 caccacatac gaggacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg
     2641 ctgcctcatc tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat
     2701 gcagaagaaa acactcggct gggaggcctt caccgaaacg ctgtaccccg ctgacggcgg
     2761 cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa
     2821 cgccaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta
     2881 ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgaaacct acgtcgagca
     2941 gcacgaggtg gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa
     3001 ttagctagcg aattcgaatt taaatcggcc tcgactgtgc cttctagttg ccagccatct
     3061 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3121 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3181 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3241 gacgcggccg caatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     3301 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3361 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3421 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3481 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3541 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     3601 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgatg
     3661 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     3721 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     3781 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     3841 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     3901 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     3961 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     4021 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     4081 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     4141 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     4201 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     4261 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     4321 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     4381 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     4441 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     4501 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     4561 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     4621 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     4681 taggtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4741 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgcgtta
     4801 actaaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4861 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4921 atcttatcat gtctggaatt gactcaaatg atgtcaatta gtctatcaga agctcatctg
     4981 gtctcccttc cgggggacaa gacatccctg tttaatattt aaacagcagt gttcccaaac
     5041 tgggttctta tatcccttgc tctggtcaac caggttgcag ggtttcctgt cctcacagga
     5101 acgaagtccc taaagaaaca gtggcagcca ggtttagccc cggaattgac tggattcctt
     5161 ttttagggcc cattggtatg gctttttccc cgtatccccc caggtgtctg caggctcaaa
     5221 gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt gcccgggctg
     5281 tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc ggagccccgg
     5341 gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg gggctttggg
     5401 ggggggctgt ccctgatatc tataacaaga aaatatatat ataataagtt atcacgtaag
     5461 tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt cacgtaaaag
     5521 ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt gacacttacc
     5581 gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc taaatgcaca
     5641 gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca tgcgtcaatt
     5701 ttacgcagac tatctttcta gggttaatct agctgcatca ggatcatatc gtcgggtctt
     5761 ttttccggct cagtcatcgc ccaagctggc gctatctggg catcggggag gaagaagccc
     5821 gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat gactgctgct
     5881 gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt gtggccatgc
     5941 acgcctttaa cggtgaactg ttcgttcagg ccacctggga taccagttcg tcgcggcttt
     6001 tccggacaca gttccggatg gtcagcccga agcgcatcag caacccgaac aataccggcg
     6061 acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg ctgggatatt
     6121 acgtcagcga ggacgggtat cctggctgga tgccgcagaa atggacatgg ataccccgtg
     6181 agttacccgg cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6241 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6301 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6361 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6421 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6481 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6541 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6601 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6661 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6721 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6781 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6841 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6901 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6961 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7021 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7081 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7141 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7201 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7261 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7321 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7381 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7441 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7501 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7561 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7621 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7681 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7741 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7801 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7861 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7921 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7981 cccggcgtca atacgggata ataccgcgcc acatagc
//