LOCUS       EW378(ZNF35/ZNF250tarv3 8058 bp ds-DNA     circular     25-FEB-2025
DEFINITION  .
KEYWORDS    "accession:urn.local...1367881019035" "marker:5'TR, 3'TR, 6, 730 R,
            Xenopus globin 5'-UTR"
FEATURES             Location/Qualifiers
     misc_feature    complement(169..1029)
                     /label="Amp"
     primer          complement(294..312)
                     /label="EKo152.2"
                     /note="sequence: tctcgcggtatcattgcag"
     primer          complement(1431..1449)
                     /label="FrtF"
                     /note="sequence: agggttccgatttagtgct"
     primer          1567..1582
                     /label="FlpInR"
                     /note="sequence: cgcgtaaccaccacac"
     primer          1737..1753
                     /label="M13-40FOR Genewiz"
                     /note="sequence: GTTTTCCCAGTCACGAC"
     primer          1757..1772
                     /label="M13F Genewiz"
                     /note="sequence: GTAAAACGACGGCCAG"
     primer_bind     1841..1860
                     /label="BII(R)"
     repeat_region   1903..1915
                     /label="5'TR"
     repeat_region   1916..1946
                     /label="5'TR(1)"
     misc_feature    1916..1946
                     /label="31bp spacer"
     repeat_region   1947..1948
                     /label="5'TR"
     repeat_region   1950..1965
                     /label="5'TR"
     enhancer        2141..2372
                     /label="core insul"
     primer          2323..2340
                     /label="EWXglobF NONSPECIFIC DONT USE"
                     /note="sequence: cttctcgctgctctttga"
     primer          2364..2379
                     /label="EWXglobF2"
                     /note="sequence: cggggaaaaggcctcc"
     primer_bind     2386..2386
                     /label="MluI-PGK-GF"
     misc_feature    2386..2386
                     /label="Added MluI site"
     misc_feature    2386..2386
                     /label="Gibson overlap"
     primer          2388..2467
                     /label="EWo119.1"
                     /note="sequence: aattgacgattgagtaacactgtatctagtctacctacagggctcAAAATTGCAAGGATATATAAAGAGctcttcAAAAT"
     primer          complement(2392..2471)
                     /label="EWo119.2"
                     /note="sequence: TGCAATTTTgaagagCTCTTTATATATCCTTGCAATTTTgagccctgtaggtagactagatacagtgttactcaatcgtc"
     misc_feature    2433..2456
                     /label="ZNF35/250 site"
     misc_feature    2463..2486
                     /label="ZNF35/250 site"
     primer          2468..2561
                     /label="EWo137.1"
                     /note="sequence: TGCAAGGATATATAAAGAGgaggtcAAAATTGCAAGGATATATAAAGAGcagaacAAAATTGCAAGGATATATAAAGAGgccttccgtatcagt"
     primer          complement(2472..2565)
                     /label="EWo137.2"
                     /note="sequence: ctagactgatacggaaggcCTCTTTATATATCCTTGCAATTTTgttctgCTCTTTATATATCCTTGCAATTTTgacctcCTCTTTATATATCCT"
     misc_feature    2493..2516
                     /label="ZNF35/250 site"
     misc_feature    2523..2546
                     /label="ZNF35/250 site"
     5'UTR           2653..2695
                     /label="Xenopus globin 5'-UTR"
     primer          complement(2682..2696)
                     /label="EWXglobR"
                     /note="sequence: gccaaagttgagcgt"
     misc_feature    2731..2731
                     /label="added SpeI site"
     misc_feature    2732..2737
                     /label="Added XhoI site"
     misc_feature    2738..2743
                     /label="Kozak"
     CDS             2744..3121
                     /label="H2B"
     CDS             2744..3853
                     /label="Translation 2744-3853"
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAITKYTSAKDPRVPVVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             3122..3139
                     /label="linker"
     primer          3123..3155
                     /label="EWo378.1"
                     /note="sequence: atccccgggtaccggtcGTCTCAAAGGGCGAGG"
     primer          3140..3158
                     /label="EKo147.1"
                     /note="sequence: GTCTCAAAGGGCGAGGAAC"
     CDS             3140..3853
                     /label="GFP"
     CDS             3140..3853
                     /label="Translation 3140-3853"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer          complement(3173..3193)
                     /label="GFP5"
                     /note="sequence: CAATTCGACGAGGATCGGAAC"
     primer          complement(3202..3231)
                     /label="NK-gfp-seq-r"
                     /note="sequence: CCACTAACTGAGAATTTATGCCCATTAACG"
     primer          3288..3306
                     /label="pNSK084"
                     /note="sequence: CCGGAAAATTGCCAGTGCC"
     primer          complement(3396..3412)
                     /label="EKo224.2"
                     /note="sequence: ACCTTCAGGCATAGCGG"
     primer          3504..3524
                     /label="pPYK028"
                     /note="sequence: GGATCGAACTGAAGGGCATAG"
     primer          3781..3800
                     /label="GFP6"
                     /note="sequence: ACGGGATCACATGGTCCTTC"
     primer          complement(3834..3871)
                     /label="EWo378.2"
                     /note="sequence: attcgaattcgctagctaCTTATACAGTTCGTCCATCC"
     CDS             3854..3856
                     /label="Translation 3854-3856"
                     /translation="*"
     misc_feature    3862..3884
                     /label="MCS"
     primer          complement(3881..3898)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
     polyA_signal    3885..4094
                     /label="BGH polyadenylation sequence"
     primer          complement(3960..3979)
                     /label="BghR1"
                     /note="sequence: attaggaaaggacagtggga"
     primer          4071..4089
                     /label="bghF"
                     /note="sequence: AGACAATAGCAGGCATGCT"
     misc_feature    4094..4095
                     /label="GATC"
     misc_feature    4096..4103
                     /label="NotI"
     primer_bind     4104..4132
                     /label="5, 299 F"
     misc_feature    4138..4138
                     /label="ES003-2 C->A"
     misc_feature    4145..4453
                     /label="pSV40"
     misc_feature    4510..5535
                     /label="Hygro"
     primer_bind     4701..4717
                     /label="2, 563 F"
     primer          4741..4762
                     /label="EKo152.1"
                     /note="sequence: cttgacattggggaattcagcg"
     primer_bind     5273..5289
                     /label="3, 135 F"
     primer          5461..5479
                     /label="Hyg_F"
                     /note="sequence: ACCGATGGCTGTGTAGAAG"
     misc_feature    complement(5515..5535)
                     /label="6, 730 R"
     misc_feature    5536..5541
                     /label="SalI"
     misc_feature    5542..5646
                     /label="WPRE"
     misc_feature    5652..5655
                     /label="SV40 pA(1)"
     misc_feature    5656..5659
                     /label="SV40 pA(1)"
     misc_feature    5657..5787
                     /label="SV40 pA"
     misc_feature    5660..5660
                     /label="SV40 pA(1)"
     misc_feature    5661..5661
                     /label="SV40 pA(1)"
     misc_feature    5662..5787
                     /label="SV40 pA(1)"
     primer          complement(5670..5695)
                     /label="SV40PA_R"
                     /note="sequence: GCTTTATTTGTAACCATTATAAGCTG"
     primer          complement(5694..5713)
                     /label="SV40pA-R Genewiz"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
     enhancer        complement(6036..6266)
                     /label="core insul(1) Geneious name: core insul(1)"
     repeat_region   6541..6575
                     /label="3'TR"
     misc_feature    6605..6605
                     /label="ES003-3 C->A"
     primer          complement(7066..7082)
                     /label="M13R Genewiz"
                     /note="sequence: CAGGAAACAGCTATGAC"
     primer          complement(7080..7099)
                     /label="M13-48REV Genewiz"
                     /note="sequence: CGGATAACAATTTCACACAG"
     primer_bind     complement(7094..7113)
                     /label="pLac(F)"
     primer          7358..7377
                     /label="pUCori5F"
                     /note="sequence: AAGGCGGTAATACGGTTATC"
     primer          complement(7529..7548)
                     /label="pUCoriR"
                     /note="sequence: tatagtcctgtcgggtttcg"
ORIGIN
        1 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
       61 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      181 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      241 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      301 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      361 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      421 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      481 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      541 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      601 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
      661 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
      721 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
      781 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
      841 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      901 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      961 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1021 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1081 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1141 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     1201 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     1261 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     1321 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     1381 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     1441 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     1501 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     1561 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     1621 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     1681 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1741 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cctcgttcat tcacgttttt
     1801 gaacccgtgg aggacgggca gactcgcggt gcaaatgtgt tttacagcgt gatggagcag
     1861 atgaagatgc tcgacacgct gcagaacacg cagctagatt aaccctagaa agataatcat
     1921 attgtgacgt acgttaaaga taatcatgtg taaaattgac gcatgtgttt tatcggtctg
     1981 tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat ttacacttac
     2041 atactaataa taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaca
     2101 aaaactcaaa atttcttcta taaagtaaca aaacttttat gagggacagc ccccccccaa
     2161 agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg
     2221 gctccgctcc ggtccggcgc tccccccgca tcccagagcc ggcagcgtgc ggggacagcc
     2281 cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga
     2341 gcctgcagac acctgggggg atacggggaa aaggcctcca cggccacaat tgacgattga
     2401 gtaacactgt atctagtcta cctacagggc tcAAAATTGC AAGGATATAT AAAGAGctct
     2461 tcAAAATTGC AAGGATATAT AAAGAGgagg tcAAAATTGC AAGGATATAT AAAGAGcaga
     2521 acAAAATTGC AAGGATATAT AAAGAGgcct tccgtatcag tctagagggt atataatgga
     2581 tcccatcgcg tctcagcctc actttgagct cctccacacg aattccccga taccgtcgat
     2641 tcaaggagct tgcttgttct ttttgcagaa gctcagaata aacgctcaac tttggcagat
     2701 ctaccaagct tggtaccgag ctcggatcca actcgaggcc accatgccag agccagcgaa
     2761 gtctgctccc gccccgaaaa agggctccaa gaaggcggtg actaaggcgc agaagaaagg
     2821 cggcaagaag cgcaagcgca gccgcaagga gagctattcc atctatgtgt acaaggttct
     2881 gaagcaggtc caccctgaca ccggcatttc gtccaaggcc atgggcatca tgaattcgtt
     2941 tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc attacaacaa
     3001 gcgctcgacc atcacctcca gggagatcca gacggccgtg cgcctgctgc tgcctgggga
     3061 gttggccaag cacgccgtgt ccgagggtac taaggccatc accaagtaca ccagcgctaa
     3121 ggatccccgg gtaccggtcG TCTCAAAGGG CGAGGAACTT TTTACGGGCG TTGTTCCGAT
     3181 CCTCGTCGAA TTGGACGGGG ACGTTAATGG GCATAAATTC TCAGTTAGTG GTGAGGGTGA
     3241 GGGGGACGCG ACATATGGGA AACTTACGCT GAAGTTCATA TGTACCACCG GAAAATTGCC
     3301 AGTGCCGTGG CCTACTCTCG TCACCACCCT GACATATGGT GTTCAGTGTT TTAGCCGGTA
     3361 TCCAGACCAT ATGAAACAGC ACGATTTTTT TAAATCCGCT ATGCCTGAAG GTTACGTACA
     3421 GGAGCGAACG ATATTTTTTA AAGACGACGG AAACTACAAA ACTAGAGCCG AGGTAAAATT
     3481 CGAGGGTGAC ACTCTCGTGA ACCGGATCGA ACTGAAGGGC ATAGATTTTA AGGAGGATGG
     3541 CAATATACTG GGCCACAAGC TCGAATACAA CTATAATTCT CATAATGTCT ATATCATGGC
     3601 GGACAAACAG AAAAACGGCA TCAAGGTAAA CTTTAAAATC CGACACAATA TTGAGGACGG
     3661 CTCTGTCCAG CTTGCGGACC ATTACCAACA GAACACGCCG ATAGGGGATG GTCCAGTACT
     3721 TCTCCCTGAT AATCACTATT TGTCTACTCA GTCAGCTCTT AGCAAGGACC CAAACGAAAA
     3781 ACGGGATCAC ATGGTCCTTC TTGAGTTCGT CACTGCGGCT GGGATAACAC TCGGGATGGA
     3841 CGAACTGTAT AAGtagctag cgaattcgaa tttaaatcgg cctcgactgt gccttctagt
     3901 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3961 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4021 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4081 aggcatgctg gggacgcggc cgcaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     4141 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4201 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4261 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     4321 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4381 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     4441 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     4501 cagcacgtga tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     4561 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     4621 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     4681 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     4741 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     4801 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     4861 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     4921 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     4981 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     5041 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     5101 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     5161 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     5221 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     5281 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     5341 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     5401 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     5461 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     5521 agggcaaagg aataggtcga caatcaacct ctggattaca aaatttgtga aagattgact
     5581 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5641 tatcatgcgt taactaaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5701 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5761 actcatcaat gtatcttatc atgtctggaa ttgactcaaa tgatgtcaat tagtctatca
     5821 gaagctcatc tggtctccct tccgggggac aagacatccc tgtttaatat ttaaacagca
     5881 gtgttcccaa actgggttct tatatccctt gctctggtca accaggttgc agggtttcct
     5941 gtcctcacag gaacgaagtc cctaaagaaa cagtggcagc caggtttagc cccggaattg
     6001 actggattcc ttttttaggg cccattggta tggctttttc cccgtatccc cccaggtgtc
     6061 tgcaggctca aagagcagcg agaagcgttc agaggaaagc gatcccgtgc caccttcccc
     6121 gtgcccgggc tgtccccgca cgctgccggc tcggggatgc ggggggagcg ccggaccgga
     6181 gcggagcccc gggcggctcg ctgctgcccc ctagcggggg agggacgtaa ttacatccct
     6241 gggggctttg ggggggggct gtccctgata tctataacaa gaaaatatat atataataag
     6301 ttatcacgta agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa
     6361 gtcacgtaaa agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca
     6421 gtgacactta ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt
     6481 cctaaatgca cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg
     6541 catgcgtcaa ttttacgcag actatctttc tagggttaat ctagctgcat caggatcata
     6601 tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg gcgctatctg ggcatcgggg
     6661 aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag tttgccgagg
     6721 atgactgctg ctgcattgac gttgagcgaa aacgcacgtt taccatgatg attcgggaag
     6781 gtgtggccat gcacgccttt aacggtgaac tgttcgttca ggccacctgg gataccagtt
     6841 cgtcgcggct tttccggaca cagttccgga tggtcagccc gaagcgcatc agcaacccga
     6901 acaataccgg cgacagccgg aactgccgtg ccggtgtgca gattaatgac agcggtgcgg
     6961 cgctgggata ttacgtcagc gaggacgggt atcctggctg gatgccgcag aaatggacat
     7021 ggataccccg tgagttaccc ggcgggcgcg cttggcgtaa tcatggtcat agctgtttcc
     7081 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7141 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7201 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7261 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7321 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7381 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7441 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7501 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7561 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7621 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7681 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7741 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7801 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7861 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7921 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7981 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8041 aaaaaaagga tctcaaga
//