LOCUS       SCN1Aprom(335-406)-H2B- 7959 bp ds-DNA     circular     07-MAR-2025
DEFINITION  .
KEYWORDS    "creator:JS Paul" "marker:5'TR, 5'TR, 5'TR, 3'TR, 6, 730 R, Xenopus
            globin 5'-UTR"
FEATURES             Location/Qualifiers
     enhancer        1..232
                     /label="core insul"
                     /note="label: core insul"
     primer_bind     246..246
                     /label="MluI-PGK-GF"
                     /note="label: MluI-PGK-GF"
     misc_feature    246..246
                     /label="Added MluI site"
                     /note="label: Added MluI site"
     misc_feature    246..246
                     /label="Gibson overlap"
                     /note="label: Gibson overlap"
     misc_feature    complement(256..279)
                     /label="JPZF1 Site"
     5'UTR           414..456
                     /label="Xenopus globin 5'-UTR"
                     /note="label: Xenopus globin 5'-UTR"
     misc_feature    492..492
                     /label="added SpeI site"
                     /note="label: added SpeI site"
     misc_feature    493..498
                     /label="Added XhoI site"
                     /note="label: Added XhoI site"
     misc_feature    499..504
                     /label="Kozak"
                     /note="label: Kozak"
     CDS             505..882
                     /label="H2B"
                     /note="label: H2B"
     CDS             505..894
                     /label="Translation 504-893"
                     /note="codon_start: 1 label: Translation 504-893 transl_table: 1 translation: MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAITKYTSAKDPRV"
     CDS             883..894
                     /label="linker"
                     /note="label: linker"
     CDS             895..900
                     /label="linker"
                     /note="label: linker"
     CDS             895..1614
                     /label="Translation 894-1613"
                     /note="codon_start: 1 label: Translation 894-1613 transl_table: 1 translation: PVVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             901..1614
                     /label="GFP"
                     /note="label: GFP"
     misc_feature    1062..1062
                     /label="a->g"
                     /note="label: a->g"
     CDS             1615..1617
                     /label="Translation 1614-1616"
                     /note="codon_start: 1 label: Translation 1614-1616 transl_table: 1 translation: *"
     misc_feature    1623..1645
                     /label="MCS"
                     /note="label: MCS"
     polyA_signal    1646..1855
                     /label="BGH polyadenylation sequence"
                     /note="label: BGH polyadenylation sequence"
     misc_feature    1855..1856
                     /label="GATC"
                     /note="label: GATC"
     misc_feature    1857..1864
                     /label="NotI"
                     /note="label: NotI"
     primer_bind     1865..1893
                     /label="5, 299 F"
                     /note="label: 5, 299 F"
     misc_feature    1899..1899
                     /label="ES003-2 C->A"
                     /note="label: ES003-2 C->A"
     misc_feature    1906..2214
                     /label="pSV40"
                     /note="label: pSV40"
     misc_feature    2271..3296
                     /label="Hygro"
                     /note="label: Hygro"
     primer_bind     2462..2478
                     /label="2, 563 F"
                     /note="label: 2, 563 F"
     primer_bind     3034..3050
                     /label="3, 135 F"
                     /note="label: 3, 135 F"
     misc_feature    complement(3276..3296)
                     /label="6, 730 R"
                     /note="label: 6, 730 R"
     misc_feature    3297..3302
                     /label="SalI"
                     /note="label: SalI"
     misc_feature    3303..3407
                     /label="WPRE"
                     /note="label: WPRE"
     misc_feature    3413..3416
                     /label="SV40 pA(1)"
                     /note="label: SV40 pA(1)"
     misc_feature    3417..3420
                     /label="SV40 pA(1)"
                     /note="label: SV40 pA(1)"
     misc_feature    3418..3548
                     /label="SV40 pA"
                     /note="label: SV40 pA"
     misc_feature    3421..3421
                     /label="SV40 pA(1)"
                     /note="label: SV40 pA(1)"
     misc_feature    3422..3422
                     /label="SV40 pA(1)"
                     /note="label: SV40 pA(1)"
     misc_feature    3423..3548
                     /label="SV40 pA(1)"
                     /note="label: SV40 pA(1)"
     enhancer        complement(3797..4027)
                     /label="core insul(1) Geneious name: core insul(1)"
                     /note="label: core insul(1) Geneious name: core insul(1)"
     repeat_region   4302..4336
                     /label="3'TR"
                     /note="label: 3'TR"
     misc_feature    4366..4366
                     /label="ES003-3 C->A"
                     /note="label: ES003-3 C->A"
     primer_bind     complement(4855..4874)
                     /label="pLac(F)"
                     /note="label: pLac(F)"
     misc_feature    complement(5988..6848)
                     /label="Amp"
                     /note="label: Amp"
     primer_bind     7660..7679
                     /label="BII(R)"
                     /note="label: BII(R)"
     repeat_region   7722..7734
                     /label="5'TR"
                     /note="label: 5'TR"
     repeat_region   7735..7765
                     /label="5'TR(1)"
                     /note="label: 5'TR(1)"
     misc_feature    7735..7765
                     /label="31bp spacer"
                     /note="label: 31bp spacer"
     repeat_region   7766..7767
                     /label="5'TR"
                     /note="label: 5'TR"
     repeat_region   7769..7784
                     /label="5'TR"
                     /note="label: 5'TR"
ORIGIN
        1 gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg
       61 gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca tcccagagcc
      121 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa
      181 cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa aaggcctcca
      241 cggccacAAT TTCCTCTCAG AGACAATTCC TTTTCCCCTC ATCCTTGTTA AATGAGCTAT
      301 CCTTTGCCTT TATACGCACA GTctagaggg tatataatgg atcccatcgc gtctcagcct
      361 cactttgagc tcctccacac gaattccccg ataccgtcga ttcaaggagc ttgcttgttc
      421 tttttgcaga agctcagaat aaacgctcaa ctttggcaga tctaccaagc ttggtaccga
      481 gctcggatcc aactcgaggc caccatgcca gagccagcga agtctgctcc cgccccgaaa
      541 aagggctcca agaaggcggt gactaaggcg cagaagaaag gcggcaagaa gcgcaagcgc
      601 agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt ccaccctgac
      661 accggcattt cgtccaaggc catgggcatc atgaattcgt ttgtgaacga cattttcgag
      721 cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac catcacctcc
      781 agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg
      841 tccgagggta ctaaggccat caccaagtac accagcgcta aggatccccg ggtaccggtc
      901 GTCTCAAAGG GCGAGGAACT TTTTACGGGC GTTGTTCCGA TCCTCGTCGA ATTGGACGGG
      961 GACGTTAATG GGCATAAATT CTCAGTTAGT GGTGAGGGTG AGGGGGACGC GACATATGGG
     1021 AAACTTACGC TGAAGTTCAT ATGTACCACC GGAAAATTGC CgGTGCCGTG GCCTACTCTC
     1081 GTCACCACCC TGACATATGG TGTTCAGTGT TTTAGCCGGT ATCCAGACCA TATGAAACAG
     1141 CACGATTTTT TTAAATCCGC TATGCCTGAA GGTTACGTAC AGGAGCGAAC GATATTTTTT
     1201 AAAGACGACG GAAACTACAA AACTAGAGCC GAGGTAAAAT TCGAGGGTGA CACTCTCGTG
     1261 AACCGGATCG AACTGAAGGG CATAGATTTT AAGGAGGATG GCAATATACT GGGCCACAAG
     1321 CTCGAATACA ACTATAATTC TCATAATGTC TATATCATGG CGGACAAACA GAAAAACGGC
     1381 ATCAAGGTAA ACTTTAAAAT CCGACACAAT ATTGAGGACG GCTCTGTCCA GCTTGCGGAC
     1441 CATTACCAAC AGAACACGCC GATAGGGGAT GGTCCAGTAC TTCTCCCTGA TAATCACTAT
     1501 TTGTCTACTC AGTCAGCTCT TAGCAAGGAC CCAAACGAAA AACGGGATCA CATGGTCCTT
     1561 CTTGAGTTCG TCACTGCGGC TGGGATAACA CTCGGGATGG ACGAACTGTA TAAGtagcta
     1621 gcgaattcga atttaaatcg gcctcgactg tgccttctag ttgccagcca tctgttgttt
     1681 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     1741 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     1801 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggacgcgg
     1861 ccgcaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     1921 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     1981 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2041 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     2101 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     2161 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     2221 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg atgaaaaagc
     2281 ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg
     2341 acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc
     2401 gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt
     2461 atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca
     2521 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
     2581 ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg
     2641 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
     2701 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
     2761 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
     2821 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
     2881 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
     2941 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
     3001 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
     3061 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
     3121 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
     3181 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
     3241 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaataggtcg
     3301 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     3361 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgcg ttaactaaac
     3421 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3481 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3541 catgtctgga attgactcaa atgatgtcaa ttagtctatc agaagctcat ctggtctccc
     3601 ttccggggga caagacatcc ctgtttaata tttaaacagc agtgttccca aactgggttc
     3661 ttatatccct tgctctggtc aaccaggttg cagggtttcc tgtcctcaca ggaacgaagt
     3721 ccctaaagaa acagtggcag ccaggtttag ccccggaatt gactggattc cttttttagg
     3781 gcccattggt atggcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc
     3841 gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc
     3901 acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc
     3961 gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt gggggggggc
     4021 tgtccctgat atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca
     4081 tgaaataaca atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca
     4141 tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga
     4201 caagcacgcc tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg
     4261 attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca
     4321 gactatcttt ctagggttaa tctagctgca tcaggatcat atcgtcgggt cttttttccg
     4381 gctcagtcat cgcccaagct ggcgctatct gggcatcggg gaggaagaag cccgtgcctt
     4441 ttcccgcgag gttgaagcgg catggaaaga gtttgccgag gatgactgct gctgcattga
     4501 cgttgagcga aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt
     4561 taacggtgaa ctgttcgttc aggccacctg ggataccagt tcgtcgcggc ttttccggac
     4621 acagttccgg atggtcagcc cgaagcgcat cagcaacccg aacaataccg gcgacagccg
     4681 gaactgccgt gccggtgtgc agattaatga cagcggtgcg gcgctgggat attacgtcag
     4741 cgaggacggg tatcctggct ggatgccgca gaaatggaca tggatacccc gtgagttacc
     4801 cggcgggcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     4861 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     4921 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     4981 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5041 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5101 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5161 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5221 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5281 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5341 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5401 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5461 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5521 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5581 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5641 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5701 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5761 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5821 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5881 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5941 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6001 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6061 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6121 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6181 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6241 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6301 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6361 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6421 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6481 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6541 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6601 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6661 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6721 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6781 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     6841 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6901 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6961 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     7021 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     7081 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     7141 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     7201 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     7261 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     7321 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     7381 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
     7441 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     7501 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     7561 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc ctcgttcatt cacgtttttg
     7621 aacccgtgga ggacgggcag actcgcggtg caaatgtgtt ttacagcgtg atggagcaga
     7681 tgaagatgct cgacacgctg cagaacacgc agctagatta accctagaaa gataatcata
     7741 ttgtgacgta cgttaaagat aatcatgtgt aaaattgacg catgtgtttt atcggtctgt
     7801 atatcgaggt ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca
     7861 tactaataat aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaacaa
     7921 aaactcaaaa tttcttctat aaagtaacaa aacttttat
//