LOCUS       JPZF1_CMV-TO-SCN1A-377( 6343 bp ds-DNA     circular     07-MAR-2025
DEFINITION  .
KEYWORDS    "creator:JS Paul"
FEATURES             Location/Qualifiers
     promoter        1..628
                     /label="CMV"
                     /note="label: CMV"
     TATA_signal     573..579
                     /label="TATA box"
                     /note="label: TATA box"
     misc_feature    589..607
                     /label="TetO"
                     /note="label: TetO"
     misc_feature    589..628
                     /label="TetR binding sites"
                     /note="label: TetR binding sites"
     misc_feature    610..628
                     /label="TetO"
                     /note="label: TetO"
     CDS             760..1431
                     /label="A104K"
                     /translation="MYECKKCGRRFGRLSNCTRHEKTHSACTGERPHGCHLCGKAFTHCSDLRKHERTHTGERPYSCGICGKSFSDSSAKRRHCILHTGERPFVCGVCKMGFSLLTSLKQHHSIHTGERPYECKECGKTFRQCSHLKRHQRIHTGERPYQCGQCGKSFRQSSNLHQHHRLHHTGERPYECKECGKTFRQCSHLKRHQRIHTGERPFECAVCGESFVNPAELADHVTVH"
     misc_feature    763..765
                     /label="mut7 Y1C "
     misc_feature    763..1431
                     /label="jpzf1"
     misc_feature    766..768
                     /label="D2E"
     misc_feature    838..840
                     /label="mut2 C26H"
                     /note="codon_start: 1 transl_table: 1"
     misc_feature    952..954
                     /label="mut3 I64E"
                     /note="codon_start: 1 transl_table: 1"
     misc_feature    1042..1044
                     /label="mut1 K94G"
                     /note="codon_start: 1 transl_table: 1"
     misc_feature    1072..1074
                     /label="A104K"
     misc_feature    1074..1074
                     /label="mut4 A104K"
                     /note="codon_start: 1 transl_table: 1"
     misc_feature    1087..1089
                     /label="S109I"
     misc_feature    1089..1089
                     /label="M21 S109I"
     misc_feature    1258..1260
                     /label="mut6 H166I"
     misc_feature    1258..1260
                     /label="mut5 H166V"
     CDS             1432..1467
                     /translation="AAMGMSGGSDGS"
     misc_feature    1432..1467
                     /label="linker"
     CDS             1468..1926
                     /translation="EGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPKQDGGWGSMAEEFVTLKDVGMDFTLGDWEQLGLEQGDTFWDTALDNCQDLFLLDPPGGGDGLGLGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDS*"
     CDS             1945..1977
                     /label="c-myc epitope"
                     /note="label: c-myc epitope"
     misc_feature    1977..1979
                     /label="New Stop"
                     /note="label: New Stop"
     CDS             1990..2007
                     /label="Polyhistidine tag"
                     /note="label: Polyhistidine tag"
     misc_feature    2008..2010
                     /label="STOP (missing now)"
                     /note="label: STOP (missing now)"
     polyA_signal    2029..2243
                     /label="BGH polyadenylation sequence"
                     /note="label: BGH polyadenylation sequence"
     polyA_signal    2276..2299
                     /label="BGH pA"
                     /note="label: BGH pA"
     misc_feature    2583..2630
                     /label="FRT"
                     /note="label: FRT"
     gene            2638..3658
                     /label="Hygromycin"
                     /note="label: Hygromycin"
     polyA_signal    3790..3920
                     /label="SV40 pA"
                     /note="label: SV40 pA"
     rep_origin      4303..4976
                     /label="pUC ori"
                     /note="label: pUC ori"
     CDS             complement(5121..5981)
                     /label="Amp (bla)"
                     /note="label: Amp (bla)"
     promoter        complement(5982..6080)
                     /label="bla promoter"
                     /note="label: bla promoter"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg
      601 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
      661 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      721 gcctccggac tctagcgttt aaacttaagc ttcgccacca tgTATGAATG CAAGAAATGT
      781 GGGCGGCGTT TCGGCCGGCT TTCCAATTGT ACACGTCACG AGAAAACCCA CAGCGCTTGT
      841 ACGGGAGAGC GCCCGCATGG CTGTCACTTG TGTGGTAAAG CCTTTACACA CTGCTCAGAT
      901 TTGAGAAAAC ACGAAAGGAC ACATACAGGT GAGCGGCCGT ACTCATGTGG AATATGTGGG
      961 AAATCCTTTT CTGATAGCAG CGCAAAGAGA CGGCACTGTA TCCTGCACAC GGGAGAACGG
     1021 CCTTTCGTCT GTGGGGTCTG CAAAATGGGA TTTTCTCTGC TTACGAGCTT AAAGCAGCAT
     1081 CACTCCATAC ATACCGGGGA ACGACCATAT GAGTGCAAAG AGTGTGGAAA GACCTTTAGG
     1141 CAATGTAGTC ACCTGAAAAG GCATCAAAGG ATACATACTG GCGAGCGGCC CTACCAATGT
     1201 GGGCAATGTG GTAAGAGCTT CAGACAATCT TCCAATCTGC ATCAGCATCA CCGCCTGCAT
     1261 CACACCGGTG AACGCCCTTA TGAATGTAAG GAATGCGGAA AGACGTTCAG ACAATGTTCA
     1321 CATCTCAAGC GGCATCAACG CATCCATACG GGAGAGAGGC CGTTCGAGTG TGCCGTCTGC
     1381 GGAGAATCTT TTGTCAATCC GGCCGAACTC GCAGACCACG TTACCGTGCA TgctgctATG
     1441 ggcATGagcg gaggctctgA cggatctGAG GGCCAGAGCG ACGAGAGAGC CCTGCTGGAC
     1501 CAGCTGCACA CCCTGCTGAG CAACACCGAC GCCACCGGCC TGGAGGAGAT CGACAGAGCC
     1561 CTGGGCATCC CCGAGCTGGT GAACCAGGGC CAGGCCCTGG AGCCCAAGCA GGACGGAGGC
     1621 TGGGGATCTA TGGCCGAGGA ATTCGTGACC CTGAAGGACG TGGGCATGGA CTTCACCCTG
     1681 GGCGACTGGG AGCAGCTGGG CCTGGAGCAG GGCGACACCT TCTGGGACAC CGCCCTGGAC
     1741 AACTGCCAGG ACCTGTTCCT GCTGGACCCC CCCGGCGGAG GAGACGGCCT TGGTTTGGGA
     1801 CACGAGAAGT TCCCCAGCGA CCTGGACCTG GACATGTTCA ACGGCAGCCT GGAGTGCGAC
     1861 ATGGAGAGCA TCATCAGAAG CGAGCTGATG GACGCCGACG GCCTGGACTT CAACTTCGAC
     1921 AGCtaggccg ctcgaggagG GCCCgaacaa aaactcatct cagaagagga tctgaatagc
     1981 gccgtcgacc atcatcataa tcatcattga gtttaaaccc gctgatcagc ctcgactgtg
     2041 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     2101 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     2161 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     2221 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg agatctatcg atgttctggg
     2281 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     2341 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2401 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2461 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2521 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtacc
     2581 tagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc cttggccaaa
     2641 aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc
     2701 tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga
     2761 gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat
     2821 gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa
     2881 ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac
     2941 ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc
     3001 gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt
     3061 caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg
     3121 caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg
     3181 ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac
     3241 aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc
     3301 ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg
     3361 gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc
     3421 cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat
     3481 ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg
     3541 actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta
     3601 gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag
     3661 cacgtactac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     3721 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     3781 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3841 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3901 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     3961 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4021 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4081 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     4141 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     4201 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4261 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4321 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4381 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4441 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4501 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4561 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4621 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     4681 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     4741 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     4801 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     4861 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     4921 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     4981 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5041 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     5101 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5161 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     5221 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     5281 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5341 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5401 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5461 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5521 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5581 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5641 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5701 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5761 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5821 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     5881 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     5941 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6001 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6061 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     6121 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     6181 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     6241 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     6301 gggttaggcg ttttgcgctg cttcgtacgg gccagatata cgc
//