LOCUS       JPZF6_CMV-TO-SCN1A-114- 6349 bp ds-DNA     circular     07-MAR-2025
DEFINITION  .
KEYWORDS    "creator:JS Paul"
FEATURES             Location/Qualifiers
     promoter        1..628
                     /label="CMV"
                     /note="label: CMV"
     TATA_signal     573..579
                     /label="TATA box"
                     /note="label: TATA box"
     misc_feature    589..607
                     /label="TetO"
                     /note="label: TetO"
     misc_feature    589..628
                     /label="TetR binding sites"
                     /note="label: TetR binding sites"
     misc_feature    610..628
                     /label="TetO"
                     /note="label: TetO"
     CDS             760..1932
                     /translation="MYQCGQCGKSFRQSSNLHQHHRLHHTGERPFICEVCHKSYTQFSNLCRHKRMHADCTGERPYQCGQCGKSFRQSSNLHQHHRLHHTGERPHECARCGKNFSWHSDLILHEQIHTGERPFVCGVCKMGFSLLTSLAQHHSSHTGERPYTCEICNKCFTRSAVLRRHKKMHCTGERPFECSKCGKACTRRCNLIQHQKVHTGERPFQCALCQKSFTQLAHLQKHHLVHAAAGGSGGSGGSEGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPKQDGGWGSMAEEFVTLKDVGMDFTLGDWEQLGLEQGDTFWDTALDNCQDLFLLDPPGGGDGLGLGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDS*"
     CDS             1951..1983
                     /label="c-myc epitope"
                     /note="label: c-myc epitope"
     misc_feature    1983..1985
                     /label="New Stop"
                     /note="label: New Stop"
     CDS             1996..2013
                     /label="Polyhistidine tag"
                     /note="label: Polyhistidine tag"
     misc_feature    2014..2016
                     /label="STOP (missing now)"
                     /note="label: STOP (missing now)"
     polyA_signal    2035..2249
                     /label="BGH polyadenylation sequence"
                     /note="label: BGH polyadenylation sequence"
     polyA_signal    2282..2305
                     /label="BGH pA"
                     /note="label: BGH pA"
     misc_feature    2589..2636
                     /label="FRT"
                     /note="label: FRT"
     gene            2644..3664
                     /label="Hygromycin"
                     /note="label: Hygromycin"
     polyA_signal    3796..3926
                     /label="SV40 pA"
                     /note="label: SV40 pA"
     rep_origin      4309..4982
                     /label="pUC ori"
                     /note="label: pUC ori"
     CDS             complement(5127..5987)
                     /label="Amp (bla)"
                     /note="label: Amp (bla)"
     promoter        complement(5988..6086)
                     /label="bla promoter"
                     /note="label: bla promoter"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg
      601 atagagatct ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga
      661 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      721 gcctccggac tCTAGCGTTT AAACTTAAGC TTCGCCACCA TGTATCAGTG TGGTCAGTGC
      781 GGTAAATCCT TCAGGCAATC TAGTAACTTA CATCAACACC ATAGGTTACA TCATACCGGG
      841 GAGAGGCCCT TTATCTGTGA GGTGTGCCAC AAGTCCTACA CTCAATTTTC CAACCTGTGT
      901 CGGCATAAAA GGATGCACGC GGATTGCACC GGAGAAAGGC CATATCAGTG CGGACAGTGT
      961 GGTAAATCTT TCCGTCAAAG CTCAAACCTG CACCAACATC ATAGGCTGCA TCACACGGGC
     1021 GAGCGGCCGC ATGAGTGTGC AAGGTGTGGT AAGAACTTTT CTTGGCATAG TGACCTCATA
     1081 CTGCATGAAC AGATTCATAC AGGGGAACGC CCGTTTGTTT GCGGCGTATG TAAAATGGGG
     1141 TTTAGCCTGT TAACTTCATT GGCTCAACAT CACAGTAGTC ACACCGGCGA AAGGCCGTAC
     1201 ACTTGCGAGA TATGTAACAA ATGTTTTACG AGGTCTGCCG TGTTGCGACG TCATAAGAAG
     1261 ATGCATTGCA CCGGAGAGCG TCCTTTTGAA TGTAGTAAAT GCGGGAAAGC ATGCACGAGA
     1321 AGATGCAACC TGATTCAACA CCAAAAGGTC CATACTGGTG AGAGGCCATT CCAATGTGCA
     1381 CTGTGTCAAA AGTCATTTAC TCAACTTGCT CATTTGCAGA AACACCATTT GGTACACGCT
     1441 GCTGCAGGCG GAAGCGGAGG CTCTGGCGGA TCtGAGGGCC AGAGCGACGA GAGAGCCCTG
     1501 CTGGACCAGC TGCACACCCT GCTGAGCAAC ACCGACGCCA CCGGCCTGGA GGAGATCGAC
     1561 AGAGCCCTGG GCATCCCCGA GCTGGTGAAC CAGGGCCAGG CCCTGGAGCC CAAGCAGGAC
     1621 GGAGGCTGGG GATCTATGGC CGAGGAATTC GTGACCCTGA AGGACGTGGG CATGGACTTC
     1681 ACCCTGGGCG ACTGGGAGCA GCTGGGCCTG GAGCAGGGCG ACACCTTCTG GGACACCGCC
     1741 CTGGACAACT GCCAGGACCT GTTCCTGCTG GACCCCCCCG GCGGAGGAGA CGGCCTTGGT
     1801 TTGGGACACG AGAAGTTCCC CAGCGACCTG GACCTGGACA TGTTCAACGG CAGCCTGGAG
     1861 TGCGACATGG AGAGCATCAT CAGAAGCGAG CTGATGGACG CCGACGGCCT GGACTTCAAC
     1921 TTCGACAGCt aggccgctcg aggagggccc gaacaaaaac tcatctcaga agaggatctg
     1981 aatagcgccg tcgaccatca tcataatcat cattgagttt aaacccgctg atcagcctcg
     2041 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     2101 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     2161 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     2221 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggagat ctatcgatgt
     2281 tctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     2341 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2401 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     2461 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     2521 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     2581 cgtacctaga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttccttg
     2641 gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     2701 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     2761 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     2821 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     2881 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     2941 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     3001 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     3061 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     3121 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     3181 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     3241 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     3301 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     3361 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     3421 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     3481 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     3541 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     3601 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     3661 gaatagcacg tactacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     3721 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     3781 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3841 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3901 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     3961 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     4021 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     4081 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     4141 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     4201 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4261 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4321 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4381 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4441 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4501 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4561 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4621 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4681 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4741 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4801 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4861 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4921 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4981 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     5041 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     5101 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     5161 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     5221 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     5281 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     5341 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     5401 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     5461 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     5521 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     5581 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     5641 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     5701 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     5761 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     5821 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5881 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     5941 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     6001 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     6061 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     6121 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     6181 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     6241 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     6301 tgcttagggt taggcgtttt gcgctgcttc gtacgggcca gatatacgc
//