LOCUS       EW370_(PB)_(ZNF35/ZNF2 10283 bp ds-DNA     circular     25-FEB-2025
DEFINITION  .
KEYWORDS    "accession:urn.local...1367881019035" "marker:5'TR, 3'TR, 6, 730 R,
            Xenopus globin 5'-UTR"
FEATURES             Location/Qualifiers
     misc_feature    complement(169..1029)
                     /label="Amp"
     primer          complement(294..312)
                     /label="EKo152.2"
                     /note="sequence: tctcgcggtatcattgcag"
     primer          complement(1431..1449)
                     /label="FrtF"
                     /note="sequence: agggttccgatttagtgct"
     primer          1567..1582
                     /label="FlpInR"
                     /note="sequence: cgcgtaaccaccacac"
     primer          1737..1753
                     /label="M13-40FOR Genewiz"
                     /note="sequence: GTTTTCCCAGTCACGAC"
     primer          1757..1772
                     /label="M13F Genewiz"
                     /note="sequence: GTAAAACGACGGCCAG"
     primer_bind     1841..1860
                     /label="BII(R)"
     repeat_region   1903..1915
                     /label="5'TR"
     repeat_region   1916..1946
                     /label="5'TR(1)"
     misc_feature    1916..1946
                     /label="31bp spacer"
     repeat_region   1947..1948
                     /label="5'TR"
     repeat_region   1950..1965
                     /label="5'TR"
     primer          1966..1988
                     /label="EW_PB_F"
                     /note="sequence: tgttttatcggtctgtatatcga"
     enhancer        2141..2372
                     /label="core insul"
     primer          2177..2198
                     /label="YX021-seq-3"
                     /note="sequence: attacgtccctcccccgctagg"
     primer          2323..2340
                     /label="EWXglobF NONSPECIFIC DONT USE"
                     /note="sequence: cttctcgctgctctttga"
     primer          2364..2379
                     /label="EWXglobF2"
                     /note="sequence: cggggaaaaggcctcc"
     primer_bind     2386..2386
                     /label="MluI-PGK-GF"
     misc_feature    2386..2386
                     /label="Gibson overlap"
     misc_feature    2386..2386
                     /label="Added MluI site"
     primer          2388..2467
                     /label="EWo119.1"
                     /note="sequence: aattgacgattgagtaacactgtatctagtctacctacagggctcAAAATTGCAAGGATATATAAAGAGctcttcAAAAT"
     primer          complement(2392..2471)
                     /label="EWo119.2"
                     /note="sequence: TGCAATTTTgaagagCTCTTTATATATCCTTGCAATTTTgagccctgtaggtagactagatacagtgttactcaatcgtc"
     misc_feature    2433..2456
                     /label="ZNF35/250 site"
     misc_feature    2463..2486
                     /label="ZNF35/250 site"
     primer          2468..2561
                     /label="EWo137.1"
                     /note="sequence: TGCAAGGATATATAAAGAGgaggtcAAAATTGCAAGGATATATAAAGAGcagaacAAAATTGCAAGGATATATAAAGAGgccttccgtatcagt"
     primer          complement(2472..2565)
                     /label="EWo137.2"
                     /note="sequence: ctagactgatacggaaggcCTCTTTATATATCCTTGCAATTTTgttctgCTCTTTATATATCCTTGCAATTTTgacctcCTCTTTATATATCCT"
     misc_feature    2493..2516
                     /label="ZNF35/250 site"
     misc_feature    2523..2546
                     /label="ZNF35/250 site"
     5'UTR           2653..2695
                     /label="Xenopus globin 5'-UTR"
     primer          complement(2682..2696)
                     /label="EWXglobR"
                     /note="sequence: gccaaagttgagcgt"
     misc_feature    2731..2731
                     /label="added SpeI site"
     misc_feature    2732..2737
                     /label="Added XhoI site"
     misc_feature    2738..2743
                     /label="Kozak"
     CDS             2744..3121
                     /label="H2B"
     CDS             2744..3847
                     /label="Translation 2744-3847"
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAITKYTSAKDPRVPVVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLN"
     CDS             2744..3850
                     /label="H2B-BFP"
     CDS             2744..3850
                     /label="H2B-GFP"
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAITKYTSAKDPRVPVVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLN*"
     CDS             3122..3139
                     /label="linker"
     misc_feature    3140..3847
                     /label="mtagBFP2"
     primer          3391..3408
                     /label="mTagBFP2.1"
                     /note="sequence: gcagtccttccctgaggg"
     CDS             3848..3850
                     /label="Translation 3848-3850"
                     /translation="*"
     misc_feature    3856..3878
                     /label="MCS"
     primer          complement(3875..3892)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
     polyA_signal    3879..4088
                     /label="BGH polyadenylation sequence"
     primer          complement(3954..3973)
                     /label="BghR1"
                     /note="sequence: attaggaaaggacagtggga"
     primer          4065..4083
                     /label="bghF"
                     /note="sequence: AGACAATAGCAGGCATGCT"
     misc_feature    4088..4089
                     /label="GATC"
     misc_feature    4090..4097
                     /label="NotI"
     primer_bind     4098..4126
                     /label="5, 299 F"
     misc_feature    4132..4132
                     /label="ES003-2 C->A"
     misc_feature    4139..4447
                     /label="pSV40"
     misc_feature    4504..5529
                     /label="Hygro"
     primer_bind     4695..4711
                     /label="2, 563 F"
     primer          4735..4756
                     /label="EKo152.1"
                     /note="sequence: cttgacattggggaattcagcg"
     primer_bind     5267..5283
                     /label="3, 135 F"
     primer          5455..5473
                     /label="Hyg_F"
                     /note="sequence: ACCGATGGCTGTGTAGAAG"
     misc_feature    complement(5509..5529)
                     /label="6, 730 R"
     misc_feature    5530..5535
                     /label="SalI"
     misc_feature    5536..5640
                     /label="WPRE"
     misc_feature    5646..5649
                     /label="SV40 pA(1)"
     misc_feature    5650..5653
                     /label="SV40 pA(1)"
     misc_feature    5651..5781
                     /label="SV40 pA"
     misc_feature    5654..5654
                     /label="SV40 pA(1)"
     misc_feature    5655..5655
                     /label="SV40 pA(1)"
     misc_feature    5656..5781
                     /label="SV40 pA(1)"
     primer          complement(5664..5689)
                     /label="SV40PA_R"
                     /note="sequence: GCTTTATTTGTAACCATTATAAGCTG"
     primer          complement(5688..5707)
                     /label="SV40pA-R Genewiz"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
     enhancer        complement(6030..6260)
                     /label="core insul(1) Geneious name: core insul(1)"
     primer          6249..6287
                     /label="EWo370.1"
                     /note="sequence: ggggctgtccctgatgttgacattgattattgactagtt"
     enhancer        6264..6356
                     /label="CMV promoter (with TetOs)"
     promoter        6264..6356
                     /label="CMV"
     promoter        6357..6891
                     /label="CMV"
     enhancer        6357..6990
                     /label="CMV promoter (with TetOs)"
     primer          6419..6438
                     /label="EK_CMVFb"
                     /note="sequence: tatgttcccatagtaacgcc"
     primer          6495..6515
                     /label="EK_CMVFc"
                     /note="sequence: tggcagtacatcaagtgtatc"
     primer          6571..6589
                     /label="EK_CMVFd"
                     /note="sequence: ttatgcccagtacatgacc"
     primer          6647..6664
                     /label="EK_CMVFe"
                     /note="sequence: gtgatgcggttttggcag"
     misc_feature    6749..6749
                     /label="A"
     primer          6801..6821
                     /label="CMV-Forward Genewiz"
                     /note="sequence: CGCAAATGGGCGGTAGGCGTG"
     TATA_signal     6836..6842
                     /label="TATA box"
     misc_feature    6852..6870
                     /label="TetO"
     misc_feature    6852..6891
                     /label="TetR binding sites"
     misc_feature    6873..6891
                     /label="TetO"
     primer          6952..6972
                     /label="CMVF1"
                     /note="sequence: tgacctccatagaagacaccg"
     primer          6973..6988
                     /label="ToFlpF"
                     /note="sequence: GGACCGATCCAGCCTC"
     primer          complement(6995..7025)
                     /label="EKo272.2"
                     /note="sequence: catggtggcgAAGCTTAAGTTTAAACGCTAG"
     primer          complement(7000..7014)
                     /label="EKo283.2"
                     /note="sequence: agcttaagtttaaac"
     misc_feature    7015..7022
                     /label="Kozak"
     misc_feature    7023..7025
                     /label="start codon"
     primer          7023..7043
                     /label="EWo50.1"
                     /note="sequence: atgTACGAGTGTAATGAGTGC"
     CDS             7023..7682
                     /translation="MYECNECGKTFTRSSNLIVHQRIHTGEKPFACNDCGKAFTQSANLIVHQRSHTGEKPYECKECGKAFSCFSHLIVHQRIHTAEKPYDCSECGKAFSQLSCLIVHQRIHTGERPYMCVECGKCFGRSSHLLQHQRIHTGEKPYVCSVCGKAFSQSSVLSKHRRIHTGEKPYECNECGKAFRVSSDLAQHHKIHTGEKPHECLECRKAFTQLSHLIQHQRIH"
     CDS             7023..8177
                     /label="ZNF35/ZNF250 TF"
     CDS             7023..8177
                     /label="ZNF35/ZNF250 TF"
                     /translation="MYECNECGKTFTRSSNLIVHQRIHTGEKPFACNDCGKAFTQSANLIVHQRSHTGEKPYECKECGKAFSCFSHLIVHQRIHTAEKPYDCSECGKAFSQLSCLIVHQRIHTGERPYMCVECGKCFGRSSHLLQHQRIHTGEKPYVCSVCGKAFSQSSVLSKHRRIHTGEKPYECNECGKAFRVSSDLAQHHKIHTGEKPHECLECRKAFTQLSHLIQHQRIHAAAWELGMSGGSEGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPKQDGGWGSMAEEFVTLKDVGMDFTLGDWEQLGLEQGDTFWDTALDNCQDLFLLDPPGGGDGLGLGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDS*"
     misc_feature    7026..7346
                     /label="ZNF35 ZF Domains 5-8"
     primer          7233..7250
                     /label="EWo51"
                     /note="sequence: AGCCACCTCATTGTACAC"
     primer          complement(7243..7259)
                     /label="EWo126"
                     /note="sequence: TATGCGCTGGTGTACAA"
     misc_feature    7347..7361
                     /label="Canonical ZF Linker"
     primer          complement(7349..7363)
                     /label="EWo100.2"
                     /note="sequence: TAGGGGCGTTCGCCC"
     primer          7349..7381
                     /label="EWo100.3"
                     /note="sequence: GGGCGAACGCCCCTATATGTGCGTAGAGTGCGG"
     misc_feature    7362..7682
                     /label="ZNF250 ZF Domains 1-4"
     primer          complement(7677..7691)
                     /label="EWo196.1"
                     /note="sequence: tgcagcagcGTGAAT"
     primer          7677..7732
                     /label="EWo196.2"
                     /note="sequence: ATTCACgctgctgcaTGGGAGCTAggaATGtctggcggatctGAGGGCCAGAGCGA"
     CDS             7683..7718
                     /label="Translation 7683-7718"
                     /translation="AAAWELGMSGGS"
     misc_feature    7719..7866
                     /label="N (NCOA3) Activation Domain"
     misc_feature    7719..8174
                     /label="NZF Domain"
     CDS             7719..8174
                     /label="Translation 7719-8174"
                     /translation="EGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPKQDGGWGSMAEEFVTLKDVGMDFTLGDWEQLGLEQGDTFWDTALDNCQDLFLLDPPGGGDGLGLGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDS"
     primer          complement(7822..7836)
                     /label="EW_NZF_R"
                     /note="sequence: GGTTCACCAGCTCGG"
     misc_feature    7881..8024
                     /label="Z (ZNF473) Activation Domain"
     misc_feature    8049..8174
                     /label="F (FOXO3) Activation Domain"
     CDS             8175..8177
                     /label="Translation 8175-8177"
                     /translation="*"
     primer          complement(8191..8212)
                     /label="pNK018"
                     /note="sequence: gagatgagtttttgttcGGGCC"
     CDS             8196..8228
                     /label="c-myc epitope"
     misc_feature    8228..8230
                     /label="New Stop"
     CDS             8241..8258
                     /label="Polyhistidine tag"
     misc_feature    8259..8261
                     /label="cya"
     primer          complement(8262..8280)
                     /label="ToFlpR"
                     /note="sequence: CTGATCAGCGGGTTTAAAC"
     polyA_signal    8280..8494
                     /label="BGH polyadenylation sequence"
     primer          complement(8281..8298)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
     primer          complement(8360..8379)
                     /label="BghR1"
                     /note="sequence: attaggaaaggacagtggga"
     primer          8471..8489
                     /label="bghF"
                     /note="sequence: AGACAATAGCAGGCATGCT"
     primer          complement(8481..8509)
                     /label="EWo370.2"
                     /note="sequence: tttcttgttatagattccccagcatgcct"
     primer          complement(8742..8765)
                     /label="EW_PB_R"
                     /note="sequence: cattcttgaaatattgctctctct"
     repeat_region   8766..8800
                     /label="3'TR"
     primer          complement(9291..9307)
                     /label="M13R Genewiz"
                     /note="sequence: CAGGAAACAGCTATGAC"
     primer          complement(9305..9324)
                     /label="M13-48REV Genewiz"
                     /note="sequence: CGGATAACAATTTCACACAG"
     primer_bind     complement(9319..9338)
                     /label="pLac(F)"
     primer          9583..9602
                     /label="pUCori5F"
                     /note="sequence: AAGGCGGTAATACGGTTATC"
     primer          complement(9754..9773)
                     /label="pUCoriR"
                     /note="sequence: tatagtcctgtcgggtttcg"
ORIGIN
        1 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
       61 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      181 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      241 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      301 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      361 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      421 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      481 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      541 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      601 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
      661 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
      721 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
      781 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
      841 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      901 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      961 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1021 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1081 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1141 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     1201 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     1261 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     1321 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     1381 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     1441 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     1501 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     1561 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     1621 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     1681 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1741 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cctcgttcat tcacgttttt
     1801 gaacccgtgg aggacgggca gactcgcggt gcaaatgtgt tttacagcgt gatggagcag
     1861 atgaagatgc tcgacacgct gcagaacacg cagctagatt aaccctagaa agataatcat
     1921 attgtgacgt acgttaaaga taatcatgtg taaaattgac gcatgtgttt tatcggtctg
     1981 tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat ttacacttac
     2041 atactaataa taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaca
     2101 aaaactcaaa atttcttcta taaagtaaca aaacttttat gagggacagc ccccccccaa
     2161 agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg
     2221 gctccgctcc ggtccggcgc tccccccgca tcccagagcc ggcagcgtgc ggggacagcc
     2281 cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga
     2341 gcctgcagac acctgggggg atacggggaa aaggcctcca cggccacaat tgacgattga
     2401 gtaacactgt atctagtcta cctacagggc tcAAAATTGC AAGGATATAT AAAGAGctct
     2461 tcAAAATTGC AAGGATATAT AAAGAGgagg tcAAAATTGC AAGGATATAT AAAGAGcaga
     2521 acAAAATTGC AAGGATATAT AAAGAGgcct tccgtatcag tctagagggt atataatgga
     2581 tcccatcgcg tctcagcctc actttgagct cctccacacg aattccccga taccgtcgat
     2641 tcaaggagct tgcttgttct ttttgcagaa gctcagaata aacgctcaac tttggcagat
     2701 ctaccaagct tggtaccgag ctcggatcca actcgaggcc accatgccag agccagcgaa
     2761 gtctgctccc gccccgaaaa agggctccaa gaaggcggtg actaaggcgc agaagaaagg
     2821 cggcaagaag cgcaagcgca gccgcaagga gagctattcc atctatgtgt acaaggttct
     2881 gaagcaggtc caccctgaca ccggcatttc gtccaaggcc atgggcatca tgaattcgtt
     2941 tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc attacaacaa
     3001 gcgctcgacc atcacctcca gggagatcca gacggccgtg cgcctgctgc tgcctgggga
     3061 gttggccaag cacgccgtgt ccgagggtac taaggccatc accaagtaca ccagcgctaa
     3121 ggatccccgg gtaccggtcg tgtctaaggg cgaagagctg attaaggaga acatgcacat
     3181 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
     3241 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
     3301 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
     3361 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
     3421 gagagtcacc acatacgagg acgggggcgt gctgaccgct acccaggaca ccagcctcca
     3481 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
     3541 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gaaacgctgt accccgctga
     3601 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
     3661 cgcaaacgcc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
     3721 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg aaacctacgt
     3781 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
     3841 gcttaattag ctagcgaatt cgaatttaaa tcggcctcga ctgtgccttc tagttgccag
     3901 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     3961 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4021 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4081 gctggggacg cggccgcaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     4141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4201 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4261 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4321 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4381 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4441 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     4501 gtgatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     4561 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     4621 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     4681 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     4741 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     4801 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     4861 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     4921 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     4981 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     5041 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     5101 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     5161 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     5221 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     5281 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     5341 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     5401 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     5461 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     5521 aaggaatagg tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5581 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5641 gcgttaacta aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5701 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5761 caatgtatct tatcatgtct ggaattgact caaatgatgt caattagtct atcagaagct
     5821 catctggtct cccttccggg ggacaagaca tccctgttta atatttaaac agcagtgttc
     5881 ccaaactggg ttcttatatc ccttgctctg gtcaaccagg ttgcagggtt tcctgtcctc
     5941 acaggaacga agtccctaaa gaaacagtgg cagccaggtt tagccccgga attgactgga
     6001 ttcctttttt agggcccatt ggtatggctt tttccccgta tccccccagg tgtctgcagg
     6061 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc
     6121 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag
     6181 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc
     6241 tttggggggg ggctgtccct gatgttgaca ttgattattg actagttatt aatagtaatc
     6301 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     6361 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     6421 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     6481 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     6541 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     6601 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     6661 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     6721 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     6781 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     6841 aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg
     6901 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     6961 tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta agcttcgcca
     7021 ccatgTACGA GTGTAATGAG TGCGGTAAGA CCTTCACACG ATCTTCTAAC CTTATTGTGC
     7081 ATCAACGAAT CCATACAGGC GAGAAGCCCT TTGCCTGCAA TGACTGTGGG AAGGCTTTTA
     7141 CTCAGAGTGC CAACTTGATC GTGCATCAGA GAAGCCATAC GGGTGAGAAG CCCTATGAAT
     7201 GCAAGGAATG CGGAAAGGCC TTCTCCTGCT TCAGCCACCT CATTGTACAC CAGCGCATAC
     7261 ACACGGCTGA GAAGCCATAC GATTGCTCTG AATGTGGAAA GGCCTTTAGT CAGCTGTCTT
     7321 GCTTGATTGT GCATCAGAGA ATTCACACGG GCGAACGCCC CTATATGTGC GTAGAGTGCG
     7381 GTAAATGTTT TGGGCGGAGT TCACATCTTT TGCAGCACCA ACGTATTCAC ACAGGCGAGA
     7441 AACCCTATGT TTGCAGCGTG TGCGGTAAGG CGTTTAGCCA GTCCTCAGTC CTTTCCAAAC
     7501 ACCGCCGCAT ACATACTGGT GAGAAGCCAT ATGAATGTAA TGAATGTGGC AAGGCTTTTA
     7561 GAGTGAGTTC AGATCTCGCT CAACATCATA AGATCCATAC AGGAGAGAAG CCACACGAAT
     7621 GTTTGGAGTG TCGAAAGGCT TTTACTCAGC TCAGTCACCT CATTCAGCAT CAGAGAATTC
     7681 ACgctgctgc aTGGGAGCTA ggaATGtctg gcggatctGA GGGCCAGAGC GACGAGAGAG
     7741 CCCTGCTGGA CCAGCTGCAC ACCCTGCTGA GCAACACCGA CGCCACCGGC CTGGAGGAGA
     7801 TCGACAGAGC CCTGGGCATC CCCGAGCTGG TGAACCAGGG CCAGGCCCTG GAGCCCAAGC
     7861 AGGACGGAGG CTGGGGATCT ATGGCCGAGG AATTCGTGAC CCTGAAGGAC GTGGGCATGG
     7921 ACTTCACCCT GGGCGACTGG GAGCAGCTGG GCCTGGAGCA GGGCGACACC TTCTGGGACA
     7981 CCGCCCTGGA CAACTGCCAG GACCTGTTCC TGCTGGACCC CCCCGGCGGA GGAGACGGCC
     8041 TTGGTTTGGG ACACGAGAAG TTCCCCAGCG ACCTGGACCT GGACATGTTC AACGGCAGCC
     8101 TGGAGTGCGA CATGGAGAGC ATCATCAGAA GCGAGCTGAT GGACGCCGAC GGCCTGGACT
     8161 TCAACTTCGA CAGCtaggcc gctcgaggag ggcccgaaca aaaactcatc tcagaagagg
     8221 atctgaatag cgccgtcgac catcatcata atcatcattg agtttaaacc cgctgatcag
     8281 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     8341 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     8401 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     8461 aggattggga agacaatagc aggcatgctg gggaatctat aacaagaaaa tatatatata
     8521 ataagttatc acgtaagtag aacatgaaat aacaatataa ttatcgtatg agttaaatct
     8581 taaaagtcac gtaaaagata atcatgcgtc attttgactc acgcggtcgt tatagttcaa
     8641 aatcagtgac acttaccgca ttgacaagca cgcctcacgg gagctccaag cggcgactga
     8701 gatgtcctaa atgcacagcg acggattcgc gctatttaga aagagagagc aatatttcaa
     8761 gaatgcatgc gtcaatttta cgcagactat ctttctaggg ttaatctagc tgcatcagga
     8821 tcatatcgtc gggtcttttt tccggctcag tcatcgccca agctggcgct atctgggcat
     8881 cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga aagagtttgc
     8941 cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca tgatgattcg
     9001 ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca cctgggatac
     9061 cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc gcatcagcaa
     9121 cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta atgacagcgg
     9181 tgcggcgctg ggatattacg tcagcgagga cgggtatcct ggctggatgc cgcagaaatg
     9241 gacatggata ccccgtgagt tacccggcgg gcgcgcttgg cgtaatcatg gtcatagctg
     9301 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9361 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     9421 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9481 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     9541 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9601 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9661 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9721 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9781 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9841 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9901 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9961 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    10021 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    10081 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    10141 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10201 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    10261 cgcagaaaaa aaggatctca aga
//