LOCUS       EW373_(PB)_(ZNF35/ZNF2 10346 bp ds-DNA     circular     26-FEB-2025
DEFINITION  .
KEYWORDS    "accession:urn.local...1367881019035" "marker:5'TR, 3'TR, 6, 730 R,
            Xenopus globin 5'-UTR"
FEATURES             Location/Qualifiers
     misc_feature    complement(169..1029)
                     /label="Amp"
     primer          complement(294..312)
                     /label="EKo152.2"
                     /note="sequence: tctcgcggtatcattgcag"
     primer          complement(1431..1449)
                     /label="FrtF"
                     /note="sequence: agggttccgatttagtgct"
     primer          1567..1582
                     /label="FlpInR"
                     /note="sequence: cgcgtaaccaccacac"
     primer          1737..1753
                     /label="M13-40FOR Genewiz"
                     /note="sequence: GTTTTCCCAGTCACGAC"
     primer          1757..1772
                     /label="M13F Genewiz"
                     /note="sequence: GTAAAACGACGGCCAG"
     primer_bind     1841..1860
                     /label="BII(R)"
     repeat_region   1903..1915
                     /label="5'TR"
     misc_feature    1916..1946
                     /label="31bp spacer"
     repeat_region   1916..1946
                     /label="5'TR(1)"
     repeat_region   1947..1948
                     /label="5'TR"
     repeat_region   1950..1965
                     /label="5'TR"
     enhancer        2141..2372
                     /label="core insul"
     primer          2323..2340
                     /label="EWXglobF NONSPECIFIC DONT USE"
                     /note="sequence: cttctcgctgctctttga"
     primer          2364..2379
                     /label="EWXglobF2"
                     /note="sequence: cggggaaaaggcctcc"
     primer_bind     2386..2386
                     /label="MluI-PGK-GF"
     misc_feature    2386..2386
                     /label="Gibson overlap"
     misc_feature    2386..2386
                     /label="Added MluI site"
     primer          2388..2467
                     /label="EWo119.1"
                     /note="sequence: aattgacgattgagtaacactgtatctagtctacctacagggctcAAAATTGCAAGGATATATAAAGAGctcttcAAAAT"
     primer          complement(2392..2471)
                     /label="EWo119.2"
                     /note="sequence: TGCAATTTTgaagagCTCTTTATATATCCTTGCAATTTTgagccctgtaggtagactagatacagtgttactcaatcgtc"
     misc_feature    2433..2456
                     /label="ZNF35/250 site"
     misc_feature    2463..2486
                     /label="ZNF35/250 site"
     primer          2468..2561
                     /label="EWo137.1"
                     /note="sequence: TGCAAGGATATATAAAGAGgaggtcAAAATTGCAAGGATATATAAAGAGcagaacAAAATTGCAAGGATATATAAAGAGgccttccgtatcagt"
     primer          complement(2472..2565)
                     /label="EWo137.2"
                     /note="sequence: ctagactgatacggaaggcCTCTTTATATATCCTTGCAATTTTgttctgCTCTTTATATATCCTTGCAATTTTgacctcCTCTTTATATATCCT"
     misc_feature    2493..2516
                     /label="ZNF35/250 site"
     misc_feature    2523..2546
                     /label="ZNF35/250 site"
     5'UTR           2653..2695
                     /label="Xenopus globin 5'-UTR"
     primer          complement(2682..2696)
                     /label="EWXglobR"
                     /note="sequence: gccaaagttgagcgt"
     misc_feature    2731..2731
                     /label="added SpeI site"
     misc_feature    2732..2737
                     /label="Added XhoI site"
     misc_feature    2738..2743
                     /label="Kozak"
     CDS             2744..3121
                     /label="H2B"
     CDS             2744..3850
                     /label="H2B-BFP"
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAITKYTSAKDPRVPVVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLN*"
     CDS             3122..3139
                     /label="linker"
     misc_feature    3140..3847
                     /label="mtagBFP2"
     primer          3391..3408
                     /label="mTagBFP2.1"
                     /note="sequence: gcagtccttccctgaggg"
     misc_feature    3856..3878
                     /label="MCS"
     primer          complement(3875..3892)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
     polyA_signal    3879..4088
                     /label="BGH polyadenylation sequence"
     primer          complement(3954..3973)
                     /label="BghR1"
                     /note="sequence: attaggaaaggacagtggga"
     primer          4065..4083
                     /label="bghF"
                     /note="sequence: AGACAATAGCAGGCATGCT"
     misc_feature    4088..4089
                     /label="GATC"
     misc_feature    4090..4097
                     /label="NotI"
     primer_bind     4098..4126
                     /label="5, 299 F"
     misc_feature    4132..4132
                     /label="ES003-2 C->A"
     misc_feature    4139..4447
                     /label="pSV40"
     misc_feature    4504..5529
                     /label="Hygro"
     primer_bind     4695..4711
                     /label="2, 563 F"
     primer          4735..4756
                     /label="EKo152.1"
                     /note="sequence: cttgacattggggaattcagcg"
     primer_bind     5267..5283
                     /label="3, 135 F"
     primer          5455..5473
                     /label="Hyg_F"
                     /note="sequence: ACCGATGGCTGTGTAGAAG"
     misc_feature    complement(5509..5529)
                     /label="6, 730 R"
     misc_feature    5530..5535
                     /label="SalI"
     misc_feature    5536..5640
                     /label="WPRE"
     misc_feature    5646..5649
                     /label="SV40 pA(1)"
     misc_feature    5650..5653
                     /label="SV40 pA(1)"
     misc_feature    5651..5781
                     /label="SV40 pA"
     misc_feature    5654..5654
                     /label="SV40 pA(1)"
     misc_feature    5655..5655
                     /label="SV40 pA(1)"
     misc_feature    5656..5781
                     /label="SV40 pA(1)"
     primer          complement(5664..5689)
                     /label="SV40PA_R"
                     /note="sequence: GCTTTATTTGTAACCATTATAAGCTG"
     primer          complement(5688..5707)
                     /label="SV40pA-R Genewiz"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
     enhancer        complement(6030..6260)
                     /label="core insul(1) Geneious name: core insul(1)"
     primer          6249..6287
                     /label="EWo370.1"
                     /note="sequence: ggggctgtccctgatgttgacattgattattgactagtt"
     promoter        6264..6356
                     /label="CMV"
     enhancer        6264..6356
                     /label="CMV promoter (with TetOs)"
     promoter        6357..6891
                     /label="CMV"
     enhancer        6357..6990
                     /label="CMV promoter (with TetOs)"
     primer          6419..6438
                     /label="EK_CMVFb"
                     /note="sequence: tatgttcccatagtaacgcc"
     primer          6495..6515
                     /label="EK_CMVFc"
                     /note="sequence: tggcagtacatcaagtgtatc"
     primer          6571..6589
                     /label="EK_CMVFd"
                     /note="sequence: ttatgcccagtacatgacc"
     primer          6647..6664
                     /label="EK_CMVFe"
                     /note="sequence: gtgatgcggttttggcag"
     misc_feature    6749..6749
                     /label="A"
     primer          6801..6821
                     /label="CMV-Forward Genewiz"
                     /note="sequence: CGCAAATGGGCGGTAGGCGTG"
     TATA_signal     6836..6842
                     /label="TATA box"
     misc_feature    6852..6870
                     /label="TetO"
     misc_feature    6852..6891
                     /label="TetR binding sites"
     misc_feature    6873..6891
                     /label="TetO"
     primer          6952..6972
                     /label="CMVF1"
                     /note="sequence: tgacctccatagaagacaccg"
     primer          6973..6988
                     /label="ToFlpF"
                     /note="sequence: GGACCGATCCAGCCTC"
     primer          complement(6995..7025)
                     /label="EKo272.2"
                     /note="sequence: catggtggcgAAGCTTAAGTTTAAACGCTAG"
     primer          complement(7000..7014)
                     /label="EKo283.2"
                     /note="sequence: agcttaagtttaaac"
     misc_feature    7015..7022
                     /label="Kozak"
     CDS             7023..8240
                     /label="GFP-NZF"
                     /translation="MGSVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKAAAWELGMSGGSEGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPKQDGGWGSMAEEFVTLKDVGMDFTLGDWEQLGLEQGDTFWDTALDNCQDLFLLDPPGGGDGLGLGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDS*"
     misc_feature    7782..7929
                     /label="N (NCOA3) Activation Domain"
     misc_feature    7782..8237
                     /label="NZF Domain"
     primer          complement(7885..7899)
                     /label="EW_NZF_R"
                     /note="sequence: GGTTCACCAGCTCGG"
     misc_feature    7944..8087
                     /label="Z (ZNF473) Activation Domain"
     misc_feature    8112..8237
                     /label="F (FOXO3) Activation Domain"
     misc_feature    8246..8264
                     /label="EWf10"
     primer          complement(8254..8275)
                     /label="pNK018"
                     /note="sequence: gagatgagtttttgttcGGGCC"
     CDS             8259..8291
                     /label="c-myc epitope"
     misc_feature    8291..8293
                     /label="New Stop"
     CDS             8304..8321
                     /label="Polyhistidine tag"
     misc_feature    8322..8324
                     /label="cya"
     primer          complement(8325..8343)
                     /label="ToFlpR"
                     /note="sequence: CTGATCAGCGGGTTTAAAC"
     polyA_signal    8343..8557
                     /label="BGH polyadenylation sequence"
     primer          complement(8344..8361)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
     primer          complement(8423..8442)
                     /label="BghR1"
                     /note="sequence: attaggaaaggacagtggga"
     primer          8534..8552
                     /label="bghF"
                     /note="sequence: AGACAATAGCAGGCATGCT"
     primer          complement(8544..8572)
                     /label="EWo370.2"
                     /note="sequence: tttcttgttatagattccccagcatgcct"
     repeat_region   8829..8863
                     /label="3'TR"
     misc_feature    8893..8893
                     /label="ES003-3 C->A"
     primer          complement(9354..9370)
                     /label="M13R Genewiz"
                     /note="sequence: CAGGAAACAGCTATGAC"
     primer          complement(9368..9387)
                     /label="M13-48REV Genewiz"
                     /note="sequence: CGGATAACAATTTCACACAG"
     primer_bind     complement(9382..9401)
                     /label="pLac(F)"
     primer          9646..9665
                     /label="pUCori5F"
                     /note="sequence: AAGGCGGTAATACGGTTATC"
     primer          complement(9817..9836)
                     /label="pUCoriR"
                     /note="sequence: tatagtcctgtcgggtttcg"
ORIGIN
        1 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
       61 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
      121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
      181 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
      241 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
      301 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
      361 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
      421 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
      481 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
      541 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
      601 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
      661 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
      721 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
      781 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
      841 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      901 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
      961 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     1021 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     1081 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     1141 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     1201 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     1261 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     1321 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     1381 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     1441 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     1501 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     1561 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     1621 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     1681 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1741 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cctcgttcat tcacgttttt
     1801 gaacccgtgg aggacgggca gactcgcggt gcaaatgtgt tttacagcgt gatggagcag
     1861 atgaagatgc tcgacacgct gcagaacacg cagctagatt aaccctagaa agataatcat
     1921 attgtgacgt acgttaaaga taatcatgtg taaaattgac gcatgtgttt tatcggtctg
     1981 tatatcgagg tttatttatt aatttgaata gatattaagt tttattatat ttacacttac
     2041 atactaataa taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaca
     2101 aaaactcaaa atttcttcta taaagtaaca aaacttttat gagggacagc ccccccccaa
     2161 agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga gccgcccggg
     2221 gctccgctcc ggtccggcgc tccccccgca tcccagagcc ggcagcgtgc ggggacagcc
     2281 cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc tgctctttga
     2341 gcctgcagac acctgggggg atacggggaa aaggcctcca cggccacaat tgacgattga
     2401 gtaacactgt atctagtcta cctacagggc tcAAAATTGC AAGGATATAT AAAGAGctct
     2461 tcAAAATTGC AAGGATATAT AAAGAGgagg tcAAAATTGC AAGGATATAT AAAGAGcaga
     2521 acAAAATTGC AAGGATATAT AAAGAGgcct tccgtatcag tctagagggt atataatgga
     2581 tcccatcgcg tctcagcctc actttgagct cctccacacg aattccccga taccgtcgat
     2641 tcaaggagct tgcttgttct ttttgcagaa gctcagaata aacgctcaac tttggcagat
     2701 ctaccaagct tggtaccgag ctcggatcca actcgaggcc accatgccag agccagcgaa
     2761 gtctgctccc gccccgaaaa agggctccaa gaaggcggtg actaaggcgc agaagaaagg
     2821 cggcaagaag cgcaagcgca gccgcaagga gagctattcc atctatgtgt acaaggttct
     2881 gaagcaggtc caccctgaca ccggcatttc gtccaaggcc atgggcatca tgaattcgtt
     2941 tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc attacaacaa
     3001 gcgctcgacc atcacctcca gggagatcca gacggccgtg cgcctgctgc tgcctgggga
     3061 gttggccaag cacgccgtgt ccgagggtac taaggccatc accaagtaca ccagcgctaa
     3121 ggatccccgg gtaccggtcg tgtctaaggg cgaagagctg attaaggaga acatgcacat
     3181 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
     3241 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
     3301 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
     3361 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
     3421 gagagtcacc acatacgagg acgggggcgt gctgaccgct acccaggaca ccagcctcca
     3481 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
     3541 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gaaacgctgt accccgctga
     3601 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
     3661 cgcaaacgcc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
     3721 cgtctactat gtggactaca gactggaaag aatcaaggag gccaacaacg aaacctacgt
     3781 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
     3841 gcttaattag ctagcgaatt cgaatttaaa tcggcctcga ctgtgccttc tagttgccag
     3901 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     3961 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4021 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4081 gctggggacg cggccgcaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     4141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4201 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4261 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     4321 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     4381 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     4441 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     4501 gtgatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     4561 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     4621 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     4681 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     4741 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     4801 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     4861 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     4921 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     4981 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     5041 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     5101 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     5161 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     5221 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     5281 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     5341 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     5401 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     5461 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     5521 aaggaatagg tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5581 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5641 gcgttaacta aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5701 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5761 caatgtatct tatcatgtct ggaattgact caaatgatgt caattagtct atcagaagct
     5821 catctggtct cccttccggg ggacaagaca tccctgttta atatttaaac agcagtgttc
     5881 ccaaactggg ttcttatatc ccttgctctg gtcaaccagg ttgcagggtt tcctgtcctc
     5941 acaggaacga agtccctaaa gaaacagtgg cagccaggtt tagccccgga attgactgga
     6001 ttcctttttt agggcccatt ggtatggctt tttccccgta tccccccagg tgtctgcagg
     6061 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc
     6121 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag
     6181 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc
     6241 tttggggggg ggctgtccct gatgttgaca ttgattattg actagttatt aatagtaatc
     6301 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     6361 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     6421 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     6481 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     6541 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     6601 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     6661 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     6721 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     6781 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     6841 aagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag atcgtcgacg
     6901 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     6961 tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta agcttcgcca
     7021 ccATGggatc cGTCTCAAAG GGCGAGGAAC TTTTTACGGG CGTTGTTCCG ATCCTCGTCG
     7081 AATTGGACGG GGACGTTAAT GGGCATAAAT TCTCAGTTAG TGGTGAGGGT GAGGGGGACG
     7141 CGACATATGG GAAACTTACG CTGAAGTTCA TATGTACCAC CGGAAAATTG CCAGTGCCGT
     7201 GGCCTACTCT CGTCACCACC CTGACATATG GTGTTCAGTG TTTTAGCCGG TATCCAGACC
     7261 ATATGAAACA GCACGATTTT TTTAAATCCG CTATGCCTGA AGGTTACGTA CAGGAGCGAA
     7321 CGATATTTTT TAAAGACGAC GGAAACTACA AAACTAGAGC CGAGGTAAAA TTCGAGGGTG
     7381 ACACTCTCGT GAACCGGATC GAACTGAAGG GCATAGATTT TAAGGAGGAT GGCAATATAC
     7441 TGGGCCACAA GCTCGAATAC AACTATAATT CTCATAATGT CTATATCATG GCGGACAAAC
     7501 AGAAAAACGG CATCAAGGTA AACTTTAAAA TCCGACACAA TATTGAGGAC GGCTCTGTCC
     7561 AGCTTGCGGA CCATTACCAA CAGAACACGC CGATAGGGGA TGGTCCAGTA CTTCTCCCTG
     7621 ATAATCACTA TTTGTCTACT CAGTCAGCTC TTAGCAAGGA CCCAAACGAA AAACGGGATC
     7681 ACATGGTCCT TCTTGAGTTC GTCACTGCGG CTGGGATAAC ACTCGGGATG GACGAACTGT
     7741 ATAAGgctgc tgcaTGGGAG CTAggaATGt ctggcggatc tGAGGGCCAG AGCGACGAGA
     7801 GAGCCCTGCT GGACCAGCTG CACACCCTGC TGAGCAACAC CGACGCCACC GGCCTGGAGG
     7861 AGATCGACAG AGCCCTGGGC ATCCCCGAGC TGGTGAACCA GGGCCAGGCC CTGGAGCCCA
     7921 AGCAGGACGG AGGCTGGGGA TCTATGGCCG AGGAATTCGT GACCCTGAAG GACGTGGGCA
     7981 TGGACTTCAC CCTGGGCGAC TGGGAGCAGC TGGGCCTGGA GCAGGGCGAC ACCTTCTGGG
     8041 ACACCGCCCT GGACAACTGC CAGGACCTGT TCCTGCTGGA CCCCCCCGGC GGAGGAGACG
     8101 GCCTTGGTTT GGGACACGAG AAGTTCCCCA GCGACCTGGA CCTGGACATG TTCAACGGCA
     8161 GCCTGGAGTG CGACATGGAG AGCATCATCA GAAGCGAGCT GATGGACGCC GACGGCCTGG
     8221 ACTTCAACTT CGACAGCtag gccgctcgag gagggcccga acaaaaactc atctcagaag
     8281 aggatctgaa tagcgccgtc gaccatcatc ataatcatca ttgagtttaa acccgctgat
     8341 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     8401 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     8461 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     8521 gggaggattg ggaagacaat agcaggcatg ctggggaatc tataacaaga aaatatatat
     8581 ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa
     8641 tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt
     8701 caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac
     8761 tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt
     8821 caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaatct agctgcatca
     8881 ggatcatatc gtcgggtctt ttttccggct cagtcatcgc ccaagctggc gctatctggg
     8941 catcggggag gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt
     9001 tgccgaggat gactgctgct gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat
     9061 tcgggaaggt gtggccatgc acgcctttaa cggtgaactg ttcgttcagg ccacctggga
     9121 taccagttcg tcgcggcttt tccggacaca gttccggatg gtcagcccga agcgcatcag
     9181 caacccgaac aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag
     9241 cggtgcggcg ctgggatatt acgtcagcga ggacgggtat cctggctgga tgccgcagaa
     9301 atggacatgg ataccccgtg agttacccgg cgggcgcgct tggcgtaatc atggtcatag
     9361 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     9421 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     9481 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     9541 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     9601 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9661 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9721 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9781 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9841 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9901 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9961 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    10021 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10081 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    10141 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    10201 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    10261 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    10321 acgcgcagaa aaaaaggatc tcaaga
//