LOCUS       EW374_(PB)_(ZNF35/ZNF3 10338 bp ds-DNA     circular     27-FEB-2025
DEFINITION  .
KEYWORDS    "accession:urn.local...1367881019035" "marker:5'TR, 3'TR, 6, 730 R,
            Xenopus globin 5'-UTR"
FEATURES             Location/Qualifiers
     misc_feature    complement(170..1030)
                     /label="Amp"
     primer          complement(295..313)
                     /label="EKo152.2"
                     /note="sequence: tctcgcggtatcattgcag"
     primer          complement(1432..1450)
                     /label="FrtF"
                     /note="sequence: agggttccgatttagtgct"
     primer          1568..1583
                     /label="FlpInR"
                     /note="sequence: cgcgtaaccaccacac"
     primer          1738..1754
                     /label="M13-40FOR Genewiz"
                     /note="sequence: GTTTTCCCAGTCACGAC"
     primer          1758..1773
                     /label="M13F Genewiz"
                     /note="sequence: GTAAAACGACGGCCAG"
     primer_bind     1842..1861
                     /label="BII(R)"
     repeat_region   1904..1916
                     /label="5'TR"
     misc_feature    1917..1947
                     /label="31bp spacer"
     repeat_region   1917..1947
                     /label="5'TR(1)"
     repeat_region   1948..1949
                     /label="5'TR"
     repeat_region   1951..1966
                     /label="5'TR"
     enhancer        2142..2373
                     /label="core insul"
     primer          2324..2341
                     /label="EWXglobF NONSPECIFIC DONT USE"
                     /note="sequence: cttctcgctgctctttga"
     primer          2365..2380
                     /label="EWXglobF2"
                     /note="sequence: cggggaaaaggcctcc"
     primer_bind     2387..2387
                     /label="MluI-PGK-GF"
     misc_feature    2387..2387
                     /label="Added MluI site"
     misc_feature    2387..2387
                     /label="Gibson overlap"
     primer          2389..2462
                     /label="EWo107.1"
                     /note="sequence: aattgacgattgagtaacactgtatctagtctacctacagggctcATATATAAAGAGATATATAAAGAGctctt"
     primer          complement(2393..2458)
                     /label="EWo103.2"
                     /note="sequence: gCTCTTTATATATCTCTTTATATATgagccctgtaggtagactagatacagtgttactcaatcgtc"
     misc_feature    2434..2457
                     /label="ZNF35/ZNF35 site"
     primer          2463..2554
                     /label="EWo145.1"
                     /note="sequence: cATATATAAAGAGATATATAAAGAGgaggtcATATATAAAGAGATATATAAAGAGcagaacATATATAAAGAGATATATAAAGAGtatcagt"
     misc_feature    2464..2487
                     /label="ZNF35/ZNF35 site"
     misc_feature    2494..2517
                     /label="ZNF35/ZNF35 site"
     misc_feature    2524..2547
                     /label="ZNF35/ZNF35 site"
     5'UTR           2646..2688
                     /label="Xenopus globin 5'-UTR"
     primer          complement(2675..2689)
                     /label="EWXglobR"
                     /note="sequence: gccaaagttgagcgt"
     misc_feature    2724..2724
                     /label="added SpeI site"
     misc_feature    2725..2730
                     /label="Added XhoI site"
     misc_feature    2731..2736
                     /label="Kozak"
     CDS             2737..3114
                     /label="H2B"
     CDS             2737..3843
                     /label="H2B-BFP"
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAITKYTSAKDPRVPVVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLN*"
     CDS             3115..3132
                     /label="linker"
     misc_feature    3133..3840
                     /label="mtagBFP2"
     primer          3384..3401
                     /label="mTagBFP2.1"
                     /note="sequence: gcagtccttccctgaggg"
     misc_feature    3849..3871
                     /label="MCS"
     primer          complement(3868..3885)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
     polyA_signal    3872..4081
                     /label="BGH polyadenylation sequence"
     primer          complement(3947..3966)
                     /label="BghR1"
                     /note="sequence: attaggaaaggacagtggga"
     primer          4058..4076
                     /label="bghF"
                     /note="sequence: AGACAATAGCAGGCATGCT"
     misc_feature    4081..4082
                     /label="GATC"
     misc_feature    4083..4090
                     /label="NotI"
     primer_bind     4091..4119
                     /label="5, 299 F"
     misc_feature    4125..4125
                     /label="ES003-2 C->A"
     misc_feature    4132..4440
                     /label="pSV40"
     misc_feature    4497..5522
                     /label="Hygro"
     primer_bind     4688..4704
                     /label="2, 563 F"
     primer          4728..4749
                     /label="EKo152.1"
                     /note="sequence: cttgacattggggaattcagcg"
     primer_bind     5260..5276
                     /label="3, 135 F"
     primer          5448..5466
                     /label="Hyg_F"
                     /note="sequence: ACCGATGGCTGTGTAGAAG"
     misc_feature    complement(5502..5522)
                     /label="6, 730 R"
     misc_feature    5523..5528
                     /label="SalI"
     misc_feature    5529..5633
                     /label="WPRE"
     misc_feature    5639..5642
                     /label="SV40 pA(1)"
     misc_feature    5643..5646
                     /label="SV40 pA(1)"
     misc_feature    5644..5774
                     /label="SV40 pA"
     misc_feature    5647..5647
                     /label="SV40 pA(1)"
     misc_feature    5648..5648
                     /label="SV40 pA(1)"
     misc_feature    5649..5774
                     /label="SV40 pA(1)"
     primer          complement(5657..5682)
                     /label="SV40PA_R"
                     /note="sequence: GCTTTATTTGTAACCATTATAAGCTG"
     primer          complement(5681..5700)
                     /label="SV40pA-R Genewiz"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
     enhancer        complement(6023..6253)
                     /label="core insul(1) Geneious name: core insul(1)"
     primer          6242..6280
                     /label="EWo370.1"
                     /note="sequence: ggggctgtccctgatgttgacattgattattgactagtt"
     promoter        6257..6884
                     /label="CMV"
     primer          6794..6814
                     /label="CMV-Forward Genewiz"
                     /note="sequence: CGCAAATGGGCGGTAGGCGTG"
     TATA_signal     6829..6835
                     /label="TATA box"
     misc_feature    6845..6863
                     /label="TetO"
     misc_feature    6845..6884
                     /label="TetR binding sites"
     misc_feature    6866..6884
                     /label="TetO"
     primer          6945..6965
                     /label="CMVF1"
                     /note="sequence: tgacctccatagaagacaccg"
     primer          6966..6981
                     /label="ToFlpF"
                     /note="sequence: GGACCGATCCAGCCTC"
     misc_feature    7008..7015
                     /label="Kozak"
     CDS             7016..7738
                     /label="GFP"
     CDS             7016..8233
                     /label="GFP-NZF"
                     /translation="MGSVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKAAAWELGMSGGSEGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPKQDGGWGSMAEEFVTLKDVGMDFTLGDWEQLGLEQGDTFWDTALDNCQDLFLLDPPGGGDGLGLGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDS*"
     misc_feature    7019..7024
                     /label="for cloning"
     primer          7025..7043
                     /label="EKo147.1"
                     /note="sequence: GTCTCAAAGGGCGAGGAAC"
     primer          complement(7058..7078)
                     /label="GFP5"
                     /note="sequence: CAATTCGACGAGGATCGGAAC"
     primer          complement(7281..7297)
                     /label="EKo224.2"
                     /note="sequence: ACCTTCAGGCATAGCGG"
     primer          7389..7409
                     /label="pPYK028"
                     /note="sequence: GGATCGAACTGAAGGGCATAG"
     primer          7666..7685
                     /label="GFP6"
                     /note="sequence: ACGGGATCACATGGTCCTTC"
     primer          complement(7713..7737)
                     /label="CCp362 secGFP-R"
                     /note="sequence: TTATACAGTTCGTCCATCCCGAGTG"
     primer          complement(7717..7738)
                     /label="EKo460.1"
                     /note="sequence: CTTATACAGTTCGTCCATCCCG"
     primer          7733..7788
                     /label="EWo196.2"
                     /note="sequence: ATTCACgctgctgcaTGGGAGCTAggaATGtctggcggatctGAGGGCCAGAGCGA"
     primer          7775..7788
                     /label="EWo211"
                     /note="sequence: GAGGGCCAGAGCGA"
     misc_feature    7775..7922
                     /label="N (NCOA3) Activation Domain"
     misc_feature    7775..8230
                     /label="NZF Domain"
     primer          complement(7878..7892)
                     /label="EW_NZF_R"
                     /note="sequence: GGTTCACCAGCTCGG"
     misc_feature    7937..8080
                     /label="Z (ZNF473) Activation Domain"
     misc_feature    8105..8230
                     /label="F (FOXO3) Activation Domain"
     CDS             8252..8284
                     /label="c-myc epitope"
     misc_feature    8284..8286
                     /label="New Stop"
     CDS             8297..8314
                     /label="Polyhistidine tag"
     misc_feature    8315..8317
                     /label="STOP (missing now)"
     primer          complement(8318..8336)
                     /label="ToFlpR"
                     /note="sequence: CTGATCAGCGGGTTTAAAC"
     polyA_signal    8336..8550
                     /label="BGH polyadenylation sequence"
     primer          complement(8337..8354)
                     /label="BGHR Genewiz"
                     /note="sequence: TAGAAGGCACAGTCGAGG"
     primer          complement(8416..8435)
                     /label="BghR1"
                     /note="sequence: attaggaaaggacagtggga"
     primer          8527..8545
                     /label="bghF"
                     /note="sequence: AGACAATAGCAGGCATGCT"
     primer          complement(8537..8565)
                     /label="EWo370.2"
                     /note="sequence: tttcttgttatagattccccagcatgcct"
     repeat_region   8822..8856
                     /label="3'TR"
     misc_feature    8886..8886
                     /label="ES003-3 C->A"
     primer          complement(9347..9363)
                     /label="M13R Genewiz"
                     /note="sequence: CAGGAAACAGCTATGAC"
     primer          complement(9361..9380)
                     /label="M13-48REV Genewiz"
                     /note="sequence: CGGATAACAATTTCACACAG"
     primer_bind     complement(9375..9394)
                     /label="pLac(F)"
     primer          9639..9658
                     /label="pUCori5F"
                     /note="sequence: AAGGCGGTAATACGGTTATC"
     primer          complement(9810..9829)
                     /label="pUCoriR"
                     /note="sequence: tatagtcctgtcgggtttcg"
ORIGIN
        1 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
       61 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      181 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
      241 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
      301 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
      361 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
      421 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
      481 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
      541 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
      601 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
      661 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
      721 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
      781 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
      841 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      901 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      961 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     1021 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1081 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     1141 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     1201 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     1261 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     1321 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     1381 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     1441 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     1501 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     1561 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     1621 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     1681 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     1741 ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt
     1801 tgaacccgtg gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca
     1861 gatgaagatg ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca
     1921 tattgtgacg tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct
     1981 gtatatcgag gtttatttat taatttgaat agatattaag ttttattata tttacactta
     2041 catactaata ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac
     2101 aaaaactcaa aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca
     2161 aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg
     2221 ggctccgctc cggtccggcg ctccccccgc atcccagagc cggcagcgtg cggggacagc
     2281 ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg
     2341 agcctgcaga cacctggggg gatacgggga aaaggcctcc acggccacaa ttgacgattg
     2401 agtaacactg tatctagtct acctacaggg ctcATATATA AAGAGATATA TAAAGAGctc
     2461 ttcATATATA AAGAGATATA TAAAGAGgag gtcATATATA AAGAGATATA TAAAGAGcag
     2521 aacATATATA AAGAGATATA TAAAGAGtat cagtctagag ggtatataat ggatcccatc
     2581 gcgtctcagc ctcactttga gctcctccac acgaattccc cgataccgtc gattcaagga
     2641 gcttgcttgt tctttttgca gaagctcaga ataaacgctc aactttggca gatctaccaa
     2701 gcttggtacc gagctcggat ccaactcgag gccaccatgc cagagccagc gaagtctgct
     2761 cccgccccga aaaagggctc caagaaggcg gtgactaagg cgcagaagaa aggcggcaag
     2821 aagcgcaagc gcagccgcaa ggagagctat tccatctatg tgtacaaggt tctgaagcag
     2881 gtccaccctg acaccggcat ttcgtccaag gccatgggca tcatgaattc gtttgtgaac
     2941 gacattttcg agcgcatcgc aggtgaggct tcccgcctgg cgcattacaa caagcgctcg
     3001 accatcacct ccagggagat ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc
     3061 aagcacgccg tgtccgaggg tactaaggcc atcaccaagt acaccagcgc taaggatccc
     3121 cgggtaccgg tcgtgtctaa gggcgaagag ctgattaagg agaacatgca catgaagctg
     3181 tacatggagg gcaccgtgga caaccatcac ttcaagtgca catccgaggg cgaaggcaag
     3241 ccctacgagg gcacccagac catgagaatc aaggtggtcg agggcggccc tctccccttc
     3301 gccttcgaca tcctggctac tagcttcctc tacggcagca agaccttcat caaccacacc
     3361 cagggcatcc ccgacttctt caagcagtcc ttccctgagg gcttcacatg ggagagagtc
     3421 accacatacg aggacggggg cgtgctgacc gctacccagg acaccagcct ccaggacggc
     3481 tgcctcatct acaacgtcaa gatcagaggg gtgaacttca catccaacgg ccctgtgatg
     3541 cagaagaaaa cactcggctg ggaggccttc accgaaacgc tgtaccccgc tgacggcggc
     3601 ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac
     3661 gccaagacca catatagatc caagaaaccc gctaagaacc tcaagatgcc tggcgtctac
     3721 tatgtggact acagactgga aagaatcaag gaggccaaca acgaaaccta cgtcgagcag
     3781 cacgaggtgg cagtggccag atactgcgac ctccctagca aactggggca caagcttaat
     3841 tagctagcga attcgaattt aaatcggcct cgactgtgcc ttctagttgc cagccatctg
     3901 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     3961 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     4021 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     4081 acgcggccgc aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     4141 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4201 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4261 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4321 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4381 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4441 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgatga
     4501 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     4561 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     4621 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     4681 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     4741 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     4801 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     4861 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     4921 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     4981 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     5041 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     5101 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     5161 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     5221 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     5281 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     5341 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     5401 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     5461 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     5521 aggtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5581 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgcgttaa
     5641 ctaaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     5701 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     5761 tcttatcatg tctggaattg actcaaatga tgtcaattag tctatcagaa gctcatctgg
     5821 tctcccttcc gggggacaag acatccctgt ttaatattta aacagcagtg ttcccaaact
     5881 gggttcttat atcccttgct ctggtcaacc aggttgcagg gtttcctgtc ctcacaggaa
     5941 cgaagtccct aaagaaacag tggcagccag gtttagcccc ggaattgact ggattccttt
     6001 tttagggccc attggtatgg ctttttcccc gtatcccccc aggtgtctgc aggctcaaag
     6061 agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt
     6121 ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg
     6181 cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg ggctttgggg
     6241 gggggctgtc cctgatgttg acattgatta ttgactagtt attaatagta atcaattacg
     6301 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     6361 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     6421 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     6481 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     6541 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     6601 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     6661 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     6721 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     6781 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     6841 gctctcccta tcagtgatag agatctccct atcagtgata gagatcgtcg acgagctcgt
     6901 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     6961 caccgggacc gatccagcct ccggactcta gcgtttaaac ttaagcttcg ccaccATGgg
     7021 atccGTCTCA AAGGGCGAGG AACTTTTTAC GGGCGTTGTT CCGATCCTCG TCGAATTGGA
     7081 CGGGGACGTT AATGGGCATA AATTCTCAGT TAGTGGTGAG GGTGAGGGGG ACGCGACATA
     7141 TGGGAAACTT ACGCTGAAGT TCATATGTAC CACCGGAAAA TTGCCAGTGC CGTGGCCTAC
     7201 TCTCGTCACC ACCCTGACAT ATGGTGTTCA GTGTTTTAGC CGGTATCCAG ACCATATGAA
     7261 ACAGCACGAT TTTTTTAAAT CCGCTATGCC TGAAGGTTAC GTACAGGAGC GAACGATATT
     7321 TTTTAAAGAC GACGGAAACT ACAAAACTAG AGCCGAGGTA AAATTCGAGG GTGACACTCT
     7381 CGTGAACCGG ATCGAACTGA AGGGCATAGA TTTTAAGGAG GATGGCAATA TACTGGGCCA
     7441 CAAGCTCGAA TACAACTATA ATTCTCATAA TGTCTATATC ATGGCGGACA AACAGAAAAA
     7501 CGGCATCAAG GTAAACTTTA AAATCCGACA CAATATTGAG GACGGCTCTG TCCAGCTTGC
     7561 GGACCATTAC CAACAGAACA CGCCGATAGG GGATGGTCCA GTACTTCTCC CTGATAATCA
     7621 CTATTTGTCT ACTCAGTCAG CTCTTAGCAA GGACCCAAAC GAAAAACGGG ATCACATGGT
     7681 CCTTCTTGAG TTCGTCACTG CGGCTGGGAT AACACTCGGG ATGGACGAAC TGTATAAGgc
     7741 tgctgcaTGG GAGCTAggaA TGtctggcgg atctGAGGGC CAGAGCGACG AGAGAGCCCT
     7801 GCTGGACCAG CTGCACACCC TGCTGAGCAA CACCGACGCC ACCGGCCTGG AGGAGATCGA
     7861 CAGAGCCCTG GGCATCCCCG AGCTGGTGAA CCAGGGCCAG GCCCTGGAGC CCAAGCAGGA
     7921 CGGAGGCTGG GGATCTATGG CCGAGGAATT CGTGACCCTG AAGGACGTGG GCATGGACTT
     7981 CACCCTGGGC GACTGGGAGC AGCTGGGCCT GGAGCAGGGC GACACCTTCT GGGACACCGC
     8041 CCTGGACAAC TGCCAGGACC TGTTCCTGCT GGACCCCCCC GGCGGAGGAG ACGGCCTTGG
     8101 TTTGGGACAC GAGAAGTTCC CCAGCGACCT GGACCTGGAC ATGTTCAACG GCAGCCTGGA
     8161 GTGCGACATG GAGAGCATCA TCAGAAGCGA GCTGATGGAC GCCGACGGCC TGGACTTCAA
     8221 CTTCGACAGC taggccgctc gaggagggcc cgaacaaaaa ctcatctcag aagaggatct
     8281 gaatagcgcc gtcgaccatc atcataatca tcattgagtt taaacccgct gatcagcctc
     8341 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     8401 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     8461 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     8521 ttgggaagac aatagcaggc atgctgggga atctataaca agaaaatata tatataataa
     8581 gttatcacgt aagtagaaca tgaaataaca atataattat cgtatgagtt aaatcttaaa
     8641 agtcacgtaa aagataatca tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc
     8701 agtgacactt accgcattga caagcacgcc tcacgggagc tccaagcggc gactgagatg
     8761 tcctaaatgc acagcgacgg attcgcgcta tttagaaaga gagagcaata tttcaagaat
     8821 gcatgcgtca attttacgca gactatcttt ctagggttaa tctagctgca tcaggatcat
     8881 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
     8941 gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag
     9001 gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
     9061 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt
     9121 tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
     9181 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg
     9241 gcgctgggat attacgtcag cgaggacggg tatcctggct ggatgccgca gaaatggaca
     9301 tggatacccc gtgagttacc cggcgggcgc gcttggcgta atcatggtca tagctgtttc
     9361 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     9421 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     9481 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     9541 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9601 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9661 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9721 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9781 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9841 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     9901 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     9961 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    10021 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    10081 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    10141 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    10201 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    10261 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10321 gaaaaaaagg atctcaag
//