LOCUS       Exported                6129 bp ds-DNA     circular SYN 03-JUL-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    PBP-E3-HA-updated
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Don Arnold
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 7, 2025 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..359
                     /note="CMV Enhancer"
     promoter        360..1623
                     /note="beta-actin promoter"
     misc_feature    1626..1670
                     /note="intron"
     misc_feature    1731..1732
                     /note="T7 promoter"
     misc_feature    1755..1757
                     /note="Start"
     misc_feature    1758..1787
                     /note="Ph4.48"
     CDS             1794..1820
                     /codon_start=1
                     /note="HA"
                     /translation="YPYDVPDYA"
     misc_feature    1821..1832
                     /note="splint"
     CDS             1834..1838
                     /codon_start=1
                     /note="3HA"
                     /translation="L"
     CDS             1839..2009
                     /codon_start=1
                     /note="RING"
                     /translation="LRRLQEEKLCKICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCC
                     TVITFKQKIFMS"
     enhancer        2191..2792
                     /note="WPRE"
     polyA_signal    2814..2954
                     /note="BGH PolyA"
     polyA_signal    3072..3798
                     /note="beta-globin PolyA"
     misc_feature    3565..3584
                     /note="T7 promoter"
     misc_feature    3607..3609
                     /note="Start"
     misc_feature    3640..3645
                     /note="GS"
     CDS             3646..3672
                     /codon_start=1
                     /note="HA"
                     /translation="LCEIVIRSQ"
     misc_feature    3673..3684
                     /note="splint"
     misc_feature    3691..4398
                     /note="TaqRFP"
     rep_origin      complement(3800..4136)
                     /direction=LEFT
                     /note="SV40 rep ori"
     misc_feature    complement(5141..5998)
                     /note="AmpR"
     CDS             complement(5839..543)
                     /note="Amp resistance"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg ggcgagggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attcggtacc ggacaattac
     1741 tatttacaat tacaatgggc ggcccattca taaaatggat caataccgga ccctacccat
     1801 acgatgttcc agattacgct ggtagcggct ccgctagcct aaggcgccta caagaggaga
     1861 agctttgcaa aatctgtatg gatagaaata ttgctatagt ttttgttcct tgtggacatc
     1921 tggtcacttg taaacagtgt gcggaagcag ttgacaaatg tcccatgtgc tgcacagtca
     1981 ttacgttcaa gcaaaaaatt tttatgtctt aataatgtac aagtaaagcg gccccggact
     2041 cgaggccgca ggtaagtatc aaggttacaa gacaggttta aggagaccac atagaaactg
     2101 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
     2161 atccactttg cctttctctc cacaggtgtc gacaatcaac ctctggatta caaaatttgt
     2221 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2281 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2341 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2401 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2461 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2521 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2581 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     2641 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2701 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2761 tccctttggg ccgcctcccc gcctggaatt cgagctcggt acgatcagcc tcgactgtgc
     2821 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     2881 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     2941 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     3001 acaatagcag gccagctttt gttcccttta gggttaattg cgcgcttggc gctaatcatg
     3061 gtcatagctg tttccactcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     3121 gccaatgccc tggctcacaa ataccactga gatcgttttc cctctgccaa aaattatggg
     3181 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     3241 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
     3301 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc catatgctgg
     3361 ctgccatgaa caaaggtggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     3421 ccattcctta ttccatagaa aagccttgac ttgaggttag attttttttt atattttgtt
     3481 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     3541 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat gaagatccct
     3601 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3661 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     3721 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3781 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
     3841 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3901 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3961 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
     4021 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4081 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     4141 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     4201 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4261 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4321 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4381 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4441 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4501 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4561 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4621 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4681 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4741 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4801 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4861 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4921 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4981 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5041 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5101 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5161 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5221 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5281 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5341 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5401 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     5461 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5521 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5581 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5641 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5701 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     5761 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5821 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5881 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5941 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6001 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6061 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6121 tgccacctg
//
