LOCUS       Exported                8507 bp ds-DNA     circular SYN 03-JUL-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    tagRFP-GPHN.FingR-PhoCl-updated
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Don Arnold
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 7, 2025 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8507
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    24..35
                     /note="CCR5ZFL BS"
     enhancer        36..394
                     /note="CMV Enhancer"
     promoter        395..1658
                     /note="beta-actin promoter"
     intron          1661..1705
     misc_feature    1766..1777
                     /note="ILR2G1 ZFBS"
     CDS             1781..2123
                     /codon_start=1
                     /note="IL2RG1 Left Zinc Finger"
                     /translation="FQCQICMRNFSRSDTLSEHIRTHTGEKPFACDICGRKFAARSTRT
                     THTKIHTGSQKPFQCRICMRNFSRSDSLSKHIRTHTGEKPFACDICGRKFAQRSNLKVH
                     TKIHLRGSQL"
     CDS             2129..2890
                     /codon_start=1
                     /note="tagRFP-4GGGS"
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLNGGGSGGGSGGGSGGGSPW"
     CDS             2891..3158
                     /codon_start=1
                     /note="GPHN.FingR"
                     /translation="LEVKEASPTSIQISWGKYKVMVRYYRITYGETGGNSPVQEFTVPG
                     SKSTATISSLKPGVDYTITVYAVTIDHWNYQDPIPISINYRTGS"
     misc_feature    3164..3211
                     /note="4GGGS"
     misc_feature    3218..3640
                     /note="TPR3"
     misc_feature    3647..4375
                     /note="PhoCl.2C"
     CDS             3791..3793
                     /codon_start=1
                     /note="K49Q"
                     /translation="Q"
     CDS             3941..3943
                     /codon_start=1
                     /note="C99G"
                     /translation="G"
     CDS             3995..3997
                     /codon_start=1
                     /note="Y117C"
                     /translation="C"
     CDS             4079..4081
                     /codon_start=1
                     /note="R145K"
                     /translation="K"
     CDS             4082..4084
                     /codon_start=1
                     /note="N146T"
                     /translation="T"
     intron          4426..4566
     enhancer        4569..5170
                     /note="WPRE"
     polyA_signal    5192..5332
                     /note="BGH PolyA"
     polyA_signal    5450..6176
                     /note="Beta-globin PolyA"
     rep_origin      complement(6178..6514)
                     /direction=LEFT
                     /note="SV40 rep ori"
     misc_feature    complement(7519..8376)
                     /note="AmpR"
ORIGIN
        1 gtcgacattg attattgact agagtcatcc tcatcgtcga cattgattat tgactagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggg
      421 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
      481 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      541 cgcgcgccag gcggggcggg gcgggggcga gggcggggcg gggcgaggcg gagaggtgcg
      601 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      661 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc
      721 cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
      781 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
      841 tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct
      901 ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
      961 gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
     1021 ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg
     1081 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
     1141 cgcggcggtc gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc
     1201 cggcttcggg tgcggggctc cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg
     1261 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     1321 gggaggggcg cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
     1381 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctggcg
     1441 gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcgggcg aagcggtgcg
     1501 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
     1561 ttctccatct ccagcctcgg ggctgccgca gggggacggc tgccttcggg ggggacgggg
     1621 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     1681 tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca
     1741 tcattttggc aaagaattcg gtacccttcc acagagtatg tttcagtgcc agatttgcat
     1801 gcgcaacttt agccgctcag atacactctc agaacatata cgaacccata cgggagaaaa
     1861 gccattcgca tgtgatatat gtgggcgcaa attcgcggct cgaagtaccc ggacaaccca
     1921 tacgaaaatt cacactggtt cgcaaaagcc ctttcagtgt cgaatttgta tgcggaattt
     1981 ctcccggtca gatagtctta gcaaacacat ccgaacacat acgggggaaa agccattcgc
     2041 ctgcgatatt tgtggacgaa aattcgctca acgctcaaat ctaaaggtac acaccaaaat
     2101 tcatctgcgc ggcagccagc tgcccggggt gtctaagggc gaagagctga ttaaggagaa
     2161 catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc
     2221 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg
     2281 cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcagaac
     2341 cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc ctgagggctt
     2401 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
     2461 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcccatc
     2521 caacggccct gtgatgcaga agaaaacact cggctgggag gccaacaccg agatgctgta
     2581 ccccgctgac ggcggcctgg aaggcagaag cgacatggcc ctgaagctcg tgggcggggg
     2641 ccacctgatc tgcaacttca agaccacata cagatccaag aaacccgcta agaacctcaa
     2701 gatgcccggc gtctactatg tggaccacag actggaaaga atcaaggagg ccgacaaaga
     2761 gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact
     2821 ggggcacaaa cttaatggtg gaggttctgg aggcggttca ggtggaggtt ctggaggcgg
     2881 ttctccatgg ctcgaagtca aggaagcatc accaaccagc atccagatca gctggggcaa
     2941 gtacaaggtc atggttcgct actaccgcat cacctacggt gaaactggtg gcaatagccc
     3001 tgtccaggaa ttcaccgtgc ctggcagcaa gtccactgct accatcagca gcctgaaacc
     3061 tggtgtcgac tataccatca cggtgtacgc cgtcacgatc gaccactgga actaccagga
     3121 cccgatcccg atctccatca actaccgcac cggttcagct agcggtggag gttctggtgg
     3181 aggttctgga ggtggatcag gaggaggttc gagatctaag ctctcaggtg gcggagggag
     3241 cggcggaggt ggctctggag gtggtggctc agctgaagca tggtacaatc tgggaaacgc
     3301 ttattacaag cagggcgact atcaaaaagc cattgagtat taccaaaagg ctcttgagct
     3361 agaccctaat aacgccgaag cgtggtataa cctaggcaac gcatattaca agcagggaga
     3421 ctaccaaaag gcaatcgaat attaccagaa ggcactcgaa ctggacccaa ataacgcaga
     3481 ggcttggtat aatctgggaa acgcctacta taaacagggt gactaccaaa aggcgataga
     3541 ggactatcag aaggccttgg agctcgatcc gaacaatctg caacgtagtg ctggtggcgg
     3601 agggtctggt ggaggaggct caggaggagg tggcgcatca atcgatatgg tgatccctga
     3661 ctacttcaag cagagcttcc ccgagggcta cagctgggag cgcagcatga cctacgagga
     3721 cggcggcatc tgcatcgcca ccaacgacat cacaatggag ggggacagct tcatcaacaa
     3781 gatccacttc cagggcacga acttcccccc caacgggccc gtgatgcaga agaggaccgt
     3841 gggctgggag gccagcaccg agaagatgta cgagcgcgac ggcgtgctga agggcgacgt
     3901 gaagatgaag ctgctgctga agggcggcgg ccactatcgc ggcgactacc gcaccaccta
     3961 caaggtcaag cagaaaccgg taaagctgcc cgactgccac ttcgtggacc accgcatcga
     4021 gatcctgagc cacgacaagg actacaacaa ggtgaagctg tacgagcacg ccgtggccaa
     4081 gacctccacc gacagcatgg acgagctgta caagggtggc agcggtggca tggtgagcaa
     4141 gggcgaggag accattacaa gcgtgatcaa gcctgacatg aagaacaagc tgcgcatgga
     4201 gggcaacgtg aacggccacg ccttcgtgat cgagggcgag ggcagcggca agcccttcga
     4261 gggcatccag acgattgatt tggaggtgaa ggagggcgcc ccgctgccct tcgcctacga
     4321 catcctgacc accgccttcc actacggcaa ccgcgtgttc accaagtacc cacggacgcg
     4381 ttaagagagc ctgcaattga gtaaagcggc cccggactcg aggccgcagg taagtatcaa
     4441 ggttacaaga caggtttaag gagaccacat agaaactggg cttgtcgaga cagagaagac
     4501 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca
     4561 caggtgtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4621 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4681 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4741 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4801 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4861 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4921 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     4981 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5041 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5101 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5161 ctggaattcg agctcggtac gatcagcctc gactgtgcct tctagttgcc agccatctgt
     5221 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     5281 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     5341 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc cagcttttgt
     5401 tccctttagg gttaattgcg cgcttggcgc taatcatggt catagctgtt tccactcctc
     5461 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
     5521 accactgaga tcgttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     5581 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     5641 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     5701 atttggttta gagtttggca acatatgcca tatgctggct gccatgaaca aaggtggcta
     5761 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     5821 gccttgactt gaggttagat ttttttttat attttgtttg tgttattttt ttctttaaca
     5881 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     5941 ccagtcatag ctgtccctct tctcttatga agatccctcg acctgcagcc caagcttggc
     6001 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6061 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat
     6181 ccgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     6241 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6301 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6361 ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa
     6421 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6481 tgtccaaact catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca
     6541 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     6961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     7201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7501 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7561 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7621 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7681 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7741 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7801 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7861 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7921 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7981 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8041 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8101 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8161 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8221 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8281 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8341 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8401 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8461 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
