LOCUS       pQdcas9-sggfp           9784 bp    DNA     circular SYN 06-MAR-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 10, 2025 from SnapGene 8.0.1
            https://www.snapgene.com
COMMENT     Sequence Label: pQdcas9-sggfp
FEATURES             Location/Qualifiers
     source          1..9784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          982..2534
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4113."
                     /note="color: #ffffff"
                     /db_xref="taxon:1944684"
                     /organism="Bacterial expression vector pJE202"
     source          2541..2595
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4113."
                     /note="color: #ffffff"
                     /db_xref="taxon:1944684"
                     /organism="Bacterial expression vector pJE202"
     source          2541..2595
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent 
                     University,Belgium) accession number LMBP 4113."
                     /note="color: #ffffff"
                     /db_xref="taxon:1944684"
                     /organism="Bacterial expression vector pJE202"
     CDS             64..858
                     /codon_start=1
                     /label=KanR/NeoR
                     /note="color: #00ff00"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     608..628
                     /label=tIRF
                     /note="color: black; sequence: AGGATCTCGTCGTGACCCATG"
     misc_feature    661..2100
                     /label=twist
                     /note="color: #a6acb3"
     misc_feature    699..1488
                     /label=F
                     /note="color: #a6acb3"
     primer_bind     876..892
                     /label=pIdcas9tF
                     /note="color: black; sequence: GAAATGACCGACCAAGC"
     primer_bind     961..1008
                     /label=luxIRF
                     /note="color: black; sequence: 
                     TTCATCCTGAACTTAtctagattaatttaagactgcttttttaaactg"
     CDS             complement(982..1563)
                     /codon_start=1
                     /transl_table=11
                     /label=luxI
                     /note="unnamed protein product; luxI"
                     /note="color: #993366"
                     /protein_id="SJL88051.1"
                     /translation="MTIMIKKSDFLAIPSEEYKGILSLRYQVFKQRLEWDLVVENNLES
                     DEYDNSNAEYIYACDDTENVSGCWRLLPTTGDYMLKSVFPELLGQQSAPKDPNIVELSR
                     FAVGKNSSKINNSASEITMKLFEAIYKHAVSQGITEYVTVTSTAIERFLKRIKVPCHRI
                     GDKEIHVLGDTKSVVLSMPINEQFKKAVLN"
     primer_bind     complement(1056..1121)
                     /label=luxirmutbacR
                     /note="color: black; sequence: 
                     caatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaa
                     attcatg"
     primer_bind     1079..1130
                     /label=luxirbackf
                     /note="color: black; sequence: 
                     cgatgacaaggaactttaatacgctttaaaaatcgctctattgctgttgatg"
     primer_bind     1351..1372
                     /label=tIRf2
                     /note="color: black; sequence: taaacgccagcatccacttaca"
     misc_feature    1419..2068
                     /label=BF2
                     /note="color: #a6acb3"
     misc_feature    1580..1634
                     /label=yl pluxI
                     /note="color: #a6acb3"
     primer_bind     1620..1634
                     /label=luxIRR
                     /note="color: black; sequence: 
                     tacgatcctacaggtagatctttaatttttaaagtatgggcaatcaa"
     CDS             1782..2534
                     /codon_start=1
                     /transl_table=11
                     /label=luxR
                     /note="unnamed protein product; luxR"
                     /note="color: #993366"
                     /protein_id="SJL88050.1"
                     /translation="MKNINADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLAI
                     IYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSNSNHSPINWNIFENNAVNK
                     KSPNVIKEAKTSGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPLIVP
                     SLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSWDISKILGCSERTVTFHLTNAQ
                     MKLNTTNRCQSISKAILTGAIDCPYFKN"
     primer_bind     2032..2053
                     /label=luxIRIF
                     /note="color: black; sequence: attctaactccaatcattcacc"
     primer_bind     complement(2119..2139)
                     /label=luxIRIR
                     /note="color: black; sequence: tgataagacctgatgttttcg"
     primer_bind     complement(2503..2534)
                     /label=qCRISPRi_Del_dCas9_UNS1_Rev
                     /note="color: black; sequence: 
                     GAGACGAGACGAGACAGCCTGAGAATGGATGCGAGTAATGttaatttttaaagtatggg
                     caatcaattgctc; added: 2020-11-04"
     primer_bind     complement(2509..2555)
                     /label=luxIRR
                     /note="color: black; sequence: 
                     tacgatcctacaggtagatctttaatttttaaagtatgggcaatcaa"
     primer_bind     2534..2617
                     /label=pIdca9F
                     /note="color: black; sequence: 
                     GAagatctacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaa
                     taaaaaagaggagaaaggatctatgg"
     misc_feature    2541..2595
                     /label=yl pluxI
                     /note="color: #a6acb3"
     misc_feature    2541..2560
                     /label=lux box
                     /note="color: #a6acb3"
     misc_feature    2593
                     /label=+1
                     /note="color: #a6acb3"
     RBS             2596..2607
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
                     /note="color: #a6acb3"
     CDS             2614..6720
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /note="color: #993366"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(2815..2826)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(3205..3223)
                     /label=pIdcas9R
                     /note="color: black; sequence: ttgcgttaatagggttttc"
     primer_bind     6512..6534
                     /label=IR9S6
                     /note="color: black; sequence: aaccaatacgtgaacaagcagaa"
     primer_bind     6721..6749
                     /label=sgxhol
                     /note="color: black; sequence: 
                     cttcccctcgagttgacagctagctcagtcctagg"
     CDS             6727..6755
                     /codon_start=1
                     /gene="pJ23119"
                     /label=pJ23119
                     /note="color: #ff9966"
                     /translation="LTASSVLGI"
     misc_feature    6762..6781
                     /label=GFPORF1
                     /note="color: #a6acb3"
     CDS             6782..6823
                     /codon_start=1
                     /gene="cas9BIRE"
                     /label=cas9BIRE
                     /note="color: #ff9966"
                     /translation="VLELEIAS*NKASP"
     CDS             6824..6863
                     /codon_start=1
                     /gene="spy-tem"
                     /label=spy-tem
                     /note="color: #ff9966"
                     /translation="LST*KSGTESVLF"
     primer_bind     6843..6856
                     /label=sghpai
                     /note="color: black; sequence: 
                     cccgggTTAACaaaaaaagcaccgactcggtg"
     primer_bind     complement(6844..6870)
                     /label=sghpai
                     /note="color: black; sequence: 
                     cccgggTTAACaaaaaaagcaccgactcggtg"
     primer_bind     complement(6870..6896)
                     /label=qCRISPRi_Ins_sspB_UNS1_Rev
                     /note="color: black; sequence: 
                     GAGACGAGACGAGACAGCCTGAGAATGGATGCGAGTAATGACTATACTAGACTGCAGGT
                     CCTGAAG; added: 2020-11-04"
     primer_bind     6916..6934
                     /label=qCRISPRi_Del_dCas9_UNS2_Fwd
                     /note="color: black; sequence: 
                     GCTGGGAGTTCGTAGACGGAAACAAACGCAGAATCCAAGCGTTGCTGCGCAACCCAAGT
                     ; added: 2020-11-04"
     CDS             complement(6936..7610)
                     /codon_start=1
                     /product="part of replication origin"
                     /label=REP
                     /note="color: #00ff00"
                     /translation="MEISMATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAA
                     MLLHHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVS
                     AYVVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGE*QLPTG
                     PGEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                     RR"
     primer_bind     complement(7036..7054)
                     /label=IR9s7
                     /note="color: black; sequence: AAACGGAGGAATGGGAACG"
     misc_feature    7139
                     /label=mut from G
                     /note="color: #a6acb3"
     CDS             8199..9590
                     /codon_start=1
                     /product="part of replication origin"
                     /label=mob
                     /note="color: #00ff00"
                     /translation="MGQGRRPRSWSRWVCGRRLGSRWAIPVVKLVGRRSLSISLSSRVV
                     HGPSEASQPVAARGHRPHIHGLARERSDRAGRSPESKPVGRRSRRRRSRLCEAKSSEYT
                     QALSGPPEAPPCAAPVEPVGHQKGGAGMAAYAIMRCKKLAKMGNVAASLKHAYRERETP
                     NADASRTPENEHWAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQ
                     QAAFFEKAHKWLADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQ
                     MTRDQTTFAAAVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYA
                     PQGLAEKLGISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDL
                     RERLKPVLDALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPE
                     KCHLG"
     promoter        9710..9759
                     /label=KanR/NeoR_promoter
                     /note="color: #ffc0cb"
ORIGIN
        1 ccaaggatct gatggcgcag gggatcaaga tttgatcaag agacaggatg aggatcgttt
       61 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
      121 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
      181 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
      241 ctacaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
      301 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
      361 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
      421 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
      481 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
      541 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
      601 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
      661 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
      721 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
      781 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
      841 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
      901 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg gactagagaa
      961 ttcatcctga acttatctag attaatttaa gactgctttt ttaaactgtt cattaatagg
     1021 catagacaat acaaccgatt tagtatcacc taatacatga atttctttgt ctccaatacg
     1081 atgacaagga actttaatac gctttaaaaa tcgctctatt gctgttgatg ttactgttac
     1141 atattctgta ataccttgac taacagcgtg tttatatata gcttcaaata gtttcattgt
     1201 aatttcacta gcagagttat ttatctttga gctattttta cctacagcaa aacgacttaa
     1261 ttcgactata ttaggatctt tgggagcact ctgttgacca agcaattcag gaaaaacact
     1321 tttcagcata taatcacctg ttgtaggtaa taaacgccag catccactta cattttcagt
     1381 atcatcacaa gcataaatat attctgcatt tgagttatca tactcatctg attcaaggtt
     1441 attttctaca actaagtccc actcaagtct ttgcttaaac acttgataac gaagacttag
     1501 aataccttta tactcctccg atggaattgc caaaaaatcc gattttttta tcattatagt
     1561 cataccaacc tcccttgcgt ttattcgact ataacaaacc attttcttgc gtaaacctgt
     1621 acgatcctac aggtgcttat gttaagtaat tgtattccca gcgatacaat agtgtgacaa
     1681 aaatccaatt tattagaatc aaatgtcaat ccattaccgt tttaatgata tataacacgc
     1741 aaaacttgcg acaaacaata ggtaaggata aagagatggg tatgaaaaac ataaatgccg
     1801 acgacacata cagaataatt aataaaatta aagcttgtag aagcaataat gatattaatc
     1861 aatgcttatc tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt
     1921 atcctcattc tatggttaaa tctgatattt caattctaga taattaccct aaaaaatgga
     1981 ggcaatatta tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact
     2041 ccaatcattc accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc
     2101 caaatgtaat taaagaagcg aaaacatcag gtcttatcac tgggtttagt ttccctattc
     2161 atacggctaa caatggcttc ggaatgctta gttttgcaca ttcagaaaaa gacaactata
     2221 tagatagttt atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg
     2281 ataattatcg aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag
     2341 aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat
     2401 taggctgcag tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata
     2461 caacaaaccg ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat
     2521 actttaaaaa ttaaagatct acctgtagga tcgtacaggt ttacgcaaga aaatggtttg
     2581 ttatagtcga ataaaaaaga ggagaaagga tctatggata agaaatactc aataggctta
     2641 gctatcggca caaatagcgt cggatgggcg gtgatcactg atgaatataa ggttccgtct
     2701 aaaaagttca aggttctggg aaatacagac cgccacagta tcaaaaaaaa tcttataggg
     2761 gctcttttat ttgacagtgg agagacagcg gaagcgactc gtctcaaacg gacagctcgt
     2821 agaaggtata cacgtcggaa gaatcgtatt tgttatctac aggagatttt ttcaaatgag
     2881 atggcgaaag tagatgatag tttctttcat cgacttgaag agtctttttt ggtggaagaa
     2941 gacaagaagc atgaacgtca tcctattttt ggaaatatag tagatgaagt tgcttatcat
     3001 gagaaatatc caactatcta tcatctgcga aaaaaattgg tagattctac tgataaagcg
     3061 gatttgcgct taatctattt ggccttagcg catatgatta agtttcgtgg tcattttttg
     3121 attgagggag atttaaatcc tgataatagt gatgtggaca aactatttat ccagttggta
     3181 caaacctaca atcaattatt tgaagaaaac cctattaacg caagtggagt agatgctaaa
     3241 gcgattcttt ctgcacgatt gagtaaatca agacgattag aaaatctcat tgctcagctc
     3301 cccggtgaga agaaaaatgg cttatttggg aatctcattg ctttgtcatt gggtttgacc
     3361 cctaatttta aatcaaattt tgatttggca gaagatgcta aattacagct ttcaaaagat
     3421 acttacgatg atgatttaga taatttattg gcgcaaattg gagatcaata tgctgatttg
     3481 tttttggcag ctaagaattt atcagatgct attttacttt cagatatcct aagagtaaat
     3541 actgaaataa ctaaggctcc cctatcagct tcaatgatta aacgctacga tgaacatcat
     3601 caagacttga ctcttttaaa agctttagtt cgacaacaac ttccagaaaa gtataaagaa
     3661 atcttttttg atcaatcaaa aaacggatat gcaggttata ttgatggggg agctagccaa
     3721 gaagaatttt ataaatttat caaaccaatt ttagaaaaaa tggatggtac tgaggaatta
     3781 ttggtgaaac taaatcgtga agatttgctg cgcaagcaac ggacctttga caacggctct
     3841 attccccatc aaattcactt gggtgagctg catgctattt tgagaagaca agaagacttt
     3901 tatccatttt taaaagacaa tcgtgagaag attgaaaaaa tcttgacttt tcgaattcct
     3961 tattatgttg gtccattggc gcgtggcaat agtcgttttg catggatgac tcggaagtct
     4021 gaagaaacaa ttaccccatg gaattttgaa gaagttgtcg ataaaggtgc ttcagctcaa
     4081 tcatttattg aacgcatgac aaactttgat aaaaatcttc caaatgaaaa agtactacca
     4141 aaacatagtt tgctttatga gtattttacg gtttataacg aattgacaaa ggtcaaatat
     4201 gttactgaag gaatgcgaaa accagcattt ctttcaggtg aacagaagaa agccattgtt
     4261 gatttactct tcaaaacaaa tcgaaaagta accgttaagc aattaaaaga agattatttc
     4321 aaaaaaatag aatgttttga tagtgttgaa atttcaggag ttgaagatag atttaatgct
     4381 tcattaggta cctaccatga tttgctaaaa attattaaag ataaagattt tttggataat
     4441 gaagaaaatg aagatatctt agaggatatt gttttaacat tgaccttatt tgaagatagg
     4501 gagatgattg aggaaagact taaaacatat gctcacctct ttgatgataa ggtgatgaaa
     4561 cagcttaaac gtcgccgtta tactggttgg ggacgtttgt ctcgaaaatt gattaatggt
     4621 attagggata agcaatctgg caaaacaata ttagattttt tgaaatcaga tggttttgcc
     4681 aatcgcaatt ttatgcagct gatccatgat gatagtttga catttaaaga agacattcaa
     4741 aaagcacaag tgtctggaca aggcgatagt ttacatgaac atattgcaaa tttagctggt
     4801 agccctgcta ttaaaaaagg tattttacag actgtaaaag ttgttgatga attggtcaaa
     4861 gtaatggggc ggcataagcc agaaaatatc gttattgaaa tggcacgtga aaatcagaca
     4921 actcaaaagg gccagaaaaa ttcgcgagag cgtatgaaac gaatcgaaga aggtatcaaa
     4981 gaattaggaa gtcagattct taaagagcat cctgttgaaa atactcaatt gcaaaatgaa
     5041 aagctctatc tctattatct ccaaaatgga agagacatgt atgtggacca agaattagat
     5101 attaatcgtt taagtgatta tgatgtcgat gccattgttc cacaaagttt ccttaaagac
     5161 gattcaatag acaataaggt cttaacgcgt tctgataaaa atcgtggtaa atcggataac
     5221 gttccaagtg aagaagtagt caaaaagatg aaaaactatt ggagacaact tctaaacgcc
     5281 aagttaatca ctcaacgtaa gtttgataat ttaacgaaag ctgaacgtgg aggtttgagt
     5341 gaacttgata aagctggttt tatcaaacgc caattggttg aaactcgcca aatcactaag
     5401 catgtggcac aaattttgga tagtcgcatg aatactaaat acgatgaaaa tgataaactt
     5461 attcgagagg ttaaagtgat taccttaaaa tctaaattag tttctgactt ccgaaaagat
     5521 ttccaattct ataaagtacg tgagattaac aattaccatc atgcccatga tgcgtatcta
     5581 aatgccgtcg ttggaactgc tttgattaag aaatatccaa aacttgaatc ggagtttgtc
     5641 tatggtgatt ataaagttta tgatgttcgt aaaatgattg ctaagtctga gcaagaaata
     5701 ggcaaagcaa ccgcaaaata tttcttttac tctaatatca tgaacttctt caaaacagaa
     5761 attacacttg caaatggaga gattcgcaaa cgccctctaa tcgaaactaa tggggaaact
     5821 ggagaaattg tctgggataa agggcgagat tttgccacag tgcgcaaagt attgtccatg
     5881 ccccaagtca atattgtcaa gaaaacagaa gtacagacag gcggattctc caaggagtca
     5941 attttaccaa aaagaaattc ggacaagctt attgctcgta aaaaagactg ggatccaaaa
     6001 aaatatggtg gttttgatag tccaacggta gcttattcag tcctagtggt tgctaaggtg
     6061 gaaaaaggga aatcgaagaa gttaaaatcc gttaaagagt tactagggat cacaattatg
     6121 gaaagaagtt cctttgaaaa aaatccgatt gactttttag aagctaaagg atataaggaa
     6181 gttaaaaaag acttaatcat taaactacct aaatatagtc tttttgagtt agaaaacggt
     6241 cgtaaacgga tgctggctag tgccggagaa ttacaaaaag gaaatgagct ggctctgcca
     6301 agcaaatatg tgaatttttt atatttagct agtcattatg aaaagttgaa gggtagtcca
     6361 gaagataacg aacaaaaaca attgtttgtg gagcagcata agcattattt agatgagatt
     6421 attgagcaaa tcagtgaatt ttctaagcgt gttattttag cagatgccaa tttagataaa
     6481 gttcttagtg catataacaa acatagagac aaaccaatac gtgaacaagc agaaaatatt
     6541 attcatttat ttacgttgac gaatcttgga gctcccgctg cttttaaata ttttgataca
     6601 acaattgatc gtaaacgata tacgtctaca aaagaagttt tagatgccac tcttatccat
     6661 caatccatca ctggtcttta tgaaacacgc attgatttga gtcagctagg aggtgactaa
     6721 ctcgagttga cagctagctc agtcctaggt ataatactag ttgctcgagc cctggaagta
     6781 cgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
     6841 tggcaccgag tcggtgcttt ttttgttaac ttcaggacct gcagtctagt atagtctgga
     6901 acagcgcact tacgggttgc tgcgcaaccc aagtgctacc ggcgcggcag cgtgacccgt
     6961 gtcggcggct ccaacggctc gccatcgtcc agaaaacacg gctcatcggg catcggcagg
     7021 cgctgctgcc cgcgccgttc ccattcctcc gtttcggtca aggctggcag gtctggttcc
     7081 atgcccggaa tgccgggctg gctgggcggc tcctcgccgg ggccggtcgg tagttgctac
     7141 tcgcccggat acagggtcgg gatgcggcgc aggtcgccat gccccaacag cgattcgtcc
     7201 tggtcgtcgt gatcaaccac cacggcggca ctgaacaccg acaggcgcaa ctggtcgcgg
     7261 ggctggcccc acgccacgcg gtcattgacc acgtaggccg acacggtgcc ggggccgttg
     7321 agcttcacga cggagatcca gcgctcggcc accaagtcct tgactgcgta ttggaccgtc
     7381 cgcaaagaac gtccgatgag cttggaaagt gtcttctggc tgaccaccac ggcgttctgg
     7441 tggcccatct gcgccacgag gtgatgcagc agcattgccg ccgtgggttt cctcgcaata
     7501 agcccggccc acgcctcatg cgctttgcgt tccgtttgca cccagtgacc gggcttgttc
     7561 ttggcttgaa tgccgatttc tctggactgc gtggccatgc ttatctccat gcggtagggt
     7621 gccgcacggt tgcggcacca tgcgcaatca gctgcaactt ttcggcagcg cgacaacaat
     7681 tatgcgttgc gtaaaagtgg cagtcaatta cagattttct ttaacctacg caatgagcta
     7741 ttgcgggggg tgccgcaatg agctgttgcg tacccccctt ttttaagttg ttgattttta
     7801 agtctttcgc atttcgccct atatctagtt ctttggtgcc caaagaaggg cacccctgcg
     7861 gggttccccc acgccttcgg cgcggctccc cctccggcaa aaagtggccc ctccggggct
     7921 tgttgatcga ctgcgcggcc ttcggccttg cccaaggtgg cgctgccccc ttggaacccc
     7981 cgcactcgcc gccgtgaggc tcggggggca ggcgggcggg cttcgccttc gactgccccc
     8041 actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca agccgctgcg cggtcgctgc
     8101 gcgagccttg acccgccttc cacttggtgt ccaaccggca agcgaagcgc gcaggccgca
     8161 ggccggaggc ttttccccag agaaaattaa aaaaattgat ggggcaaggc cgcaggccgc
     8221 gcagttggag ccggtgggta tgtggtcgaa ggctgggtag ccggtgggca atccctgtgg
     8281 tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc cagcagggtt gtccacgggc
     8341 cgagcgaagc gagccagccg gtggccgctc gcggccatcg tccacatatc cacgggctgg
     8401 caagggagcg cagcgaccgc gcagggcgaa gcccggagag caagcccgta gggcgccgca
     8461 gccgccgtag gcggtcacga ctttgcgaag caaagtctag tgagtatact caagcattga
     8521 gtggcccgcc ggaggcaccg ccttgcgctg cccccgtcga gccggttgga caccaaaagg
     8581 gaggggcagg catggcggca tacgcgatca tgcgatgcaa gaagctggcg aaaatgggca
     8641 acgtggcggc cagtctcaag cacgcctacc gcgagcgcga gacgcccaac gctgacgcca
     8701 gcaggacgcc agagaacgag cactgggcgg ccagcagcac cgatgaagcg atgggccgac
     8761 tgcgcgagtt gctgccagag aagcggcgca aggacgctgt gttggcggtc gagtacgtca
     8821 tgacggccag cccggaatgg tggaagtcgg ccagccaaga acagcaggcg gcgttcttcg
     8881 agaaggcgca caagtggctg gcggacaagt acggggcgga tcgcatcgtg acggccagca
     8941 tccaccgtga cgaaaccagc ccgcacatga ccgcgttcgt ggtgccgctg acgcaggacg
     9001 gcaggctgtc ggccaaggag ttcatcggca acaaagcgca gatgacccgc gaccagacca
     9061 cgtttgcggc tgctgtggcc gatctagggc tgcaacgggg catcgagggc agcaaggcac
     9121 gtcacacgcg cattcaggcg ttctacgagg ccctggagcg gccaccagtg ggccacgtca
     9181 ccatcagccc gcaagcggtc gagccacgcg cctatgcacc gcagggattg gccgaaaagc
     9241 tgggaatctc aaagcgcgtt gagacgccgg aagccgtggc cgaccggctg acaaaagcgg
     9301 ttcggcaggg gtatgagcct gccctacagg ccgccgcagg agcgcgtgag atgcgcaaga
     9361 aggccgatca agcccaagag acggcccgag accttcggga gcgcctgaag cccgttctgg
     9421 acgccctggg gccgttgaat cgggatatgc aggccaaggc cgccgcgatc atcaaggccg
     9481 tgggcgaaaa gctgctgacg gaacagcggg aagtccagcg ccagaaacag gcccagcgcc
     9541 agcaggaacg cgggcgcgca catttccccg aaaagtgcca cctgggatga atgtcagcta
     9601 ctgggctatc tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg
     9661 gcttacatgg cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc
     9721 agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt
     9781 gccg
//
