LOCUS       Exported                3765 bp ds-DNA     circular SYN 05-DEC-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Yvonne Nygard
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2025 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
     protein_bind    1..4
                     /label=BsmBI
                     /note="color: #00ff15"
     misc_feature    complement(7..115)
                     /label=CamR Terminator
                     /note="color: #66ccff; direction: LEFT"
     CDS             complement(116..775)
                     /label=CamR
                     /note="color: #0000ff; direction: LEFT"
     promoter        complement(776..880)
                     /label=CamR Promoter
                     /note="color: #66ccff; direction: LEFT"
     rep_origin      complement(881..1644)
                     /direction=LEFT
                     /label=ColE1
                     /note="color: #7f7f7f"
     misc_feature    complement(1659..2394)
                     /label=MCS
                     /note="poly-linker region"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    complement(1659)
                     /label=SGI-S1
                     /note="SGI-S1"
                     /note="color: #a6acb3; direction: LEFT"
     misc_feature    1660..2377
                     /label=PGK1 Promoter
                     /note="PGK1 Promoter"
                     /note="color: #a6acb3"
     misc_feature    2388..2397
                     /label=KOZAK
                     /note="KOZAK"
                     /note="color: #a6acb3"
     misc_feature    2398..2943
                     /label=QF BD
                     /note="color: #a6acb3"
     misc_feature    2944..3444
                     /label=QF AD
                     /note="color: #a6acb3"
     misc_feature    3445..3450
                     /label=STOP
                     /note="STOP"
                     /note="color: #a6acb3"
     misc_feature    3456..3457
                     /label=MCS
                     /note="MCS"
                     /note="color: #a6acb3"
     misc_feature    3457..3462
                     /label=STOP
                     /note="STOP"
                     /note="color: #a6acb3"
     misc_feature    3466..3758
                     /label=ACT1 t
                     /note="ACT1 t"
                     /note="color: #a6acb3"
ORIGIN
        1 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
       61 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
      121 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
      181 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      241 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
      301 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
      361 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
      421 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
      481 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
      541 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
      601 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
      661 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
      721 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
      781 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
      841 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa
      901 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      961 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1021 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1081 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1141 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1201 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1261 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1321 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1381 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1441 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1501 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1561 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1621 tcccctgatt ctgtggataa ccgtagtcgg tctcaaacgc aagaattact cgtgagtaag
     1681 gaaagagtga ggaactatcg catacctgca tttaaagatg ccgatttggg cgcgaatcct
     1741 ttattttggc ttcaccctca tactattatc agggccagaa aaaggaagtg tttccctcct
     1801 tcttgaattg atgttaccct cataaagcac gtggcctctt atcgagaaag aaattaccgt
     1861 cgctcgtgat ttgtttgcaa aaagaacaaa actgaaaaaa cccagacacg ctcgacttcc
     1921 tgtcttccta ttgattgcag cttccaattt cgtcacacaa caaggtccta gcgacggctc
     1981 acaggttttg taacaagcaa tcgaaggttc tggaatggcg ggaaagggtt tagtaccaca
     2041 tgctatgatg cccactgtga tctccagagc aaagttcgtt cgatcgtact gttactctct
     2101 ctctttcaaa cagaattgtc cgaatcgtgt gacaacaaca gcctgttctc acacactctt
     2161 ttcttctaac caagggggtg gtttagttta gtagaacctc gtgaaactta catttacata
     2221 tatataaact tgcataaatt ggtcaatgca agaaatacat atttggtctt ttctaattcg
     2281 tagtttttca agttcttaga tgctttcttt ttctcttttt tacagatcat caaggaagta
     2341 attatctact ttttacaaca aatataaaac accgcggtgg cggccgctaa aatcatgcca
     2401 cccaagagaa agacgctgaa cgcggcagcg gaagctaacg cccatgcaga tggccacgcg
     2461 gacggtaatg cggacggtca tgtagctaac accgcggctt cctctaacaa cgcacgtttc
     2521 gctgacttga cgaatatcga tacgccaggg ttgggaccga ctactaccac cttattggtg
     2581 gagcctgcca ggtctaaaag acaaagggtc agtcgtgcgt gtgaccaatg cagagccgct
     2641 cgtgaaaaat gtgacggtat tcagcctgca tgttttcctt gcgtgagtca gggcaggtct
     2701 tgcacgtatc aggcttcccc caagaagcgt ggcgttcaga ctggttatat taggaccttg
     2761 gaattggcgt tggcgtggat gtttgaaaac gtagcaaggt ccgaagacgc gctacataat
     2821 ctattagtca gggacgctgg ccaaggctca gcgttactgg tagggaagga ttcaccggcg
     2881 gcggaaagac tgcatgcaag gtgggcgacc tccagggtca ataaaagtat cacaaggctt
     2941 ctccgtcaat tagagctgcc gcccacggct actgccactg cgtcaatcat gcctcacgtc
     3001 atggaacaac ccttgagtac gtcaataaac cctgttaatg atcgttttaa tgggattcca
     3061 aatccgacgc cttacaattc tgacgctgct cttgacgcta taacccaaac caacgattac
     3121 ggttccgtga atacccacgg aatcctgagc acctaccccc ctccagcaac acaccttaac
     3181 gaggcgtcag tagctctagc tccgggaggg gcgcctccgc gtccaccccc accgtacgtg
     3241 gactcaacga ctaatcaccc tccctatcat agcaacctgg tccctatggc aaattttggt
     3301 tacagtacag ttgattatga tgccatggta gatgatttag cgagtattga atatactgat
     3361 gctgtagatg ttgacccaca atttatgacg aatcttgggt tcgtccctgg gtgcaatttc
     3421 agtgacatta acacgtatga acaataatga ctcgagtaat gatcatctct gcttttgtgc
     3481 gcgtatgttt atgtatgtac ctctctctct atttctattt ttaaaccacc ctctcaataa
     3541 aataaaaata ataaagtatt tttaaggaaa agacgtgttt aagcactgac tttatctact
     3601 ttttgtacgt tttcattgat ataatgtgtt ttgtctctcc cttttctacg aaaatttcaa
     3661 aaattgacca aaaaaaggaa tatatatacg aaaaactatt atatttatat atcatagtgt
     3721 tgataaaaaa tgtttatcca ttggaccgtg tatcatatgc tgtga
//
