LOCUS       Exported                5239 bp ds-DNA     circular SYN 05-DEC-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Yvonne Nygard
  TITLE     Direct Submission
  JOURNAL   Exported Dec 5, 2025 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     protein_bind    1..4
                     /label=BsaI
                     /note="color: #00ff2c"
     terminator      complement(13..39)
                     /label=KanR Terminator
                     /note="color: #93ff35; direction: LEFT"
     CDS             complement(40..855)
                     /label=KanR
                     /note="color: #008000; direction: LEFT"
     promoter        complement(856..1003)
                     /label=KanR Promoter
                     /note="color: #93ff35; direction: LEFT"
     rep_origin      complement(1004..1767)
                     /direction=LEFT
                     /label=ColE1
                     /note="color: #7f7f7f"
     protein_bind    1776..1779
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    1780..2279
                     /label=HO 5' Homology
                     /note="color: #6685ca"
     misc_feature    complement(2280..2299)
                     /label=HO 5' barcode
                     /note="color: #8c3031; direction: LEFT"
     protein_bind    2300..2303
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    2319..2343
                     /label=ConS scar
                     /note="color: #fffd6c"
     protein_bind    2319..2322
                     /label=BsmBI
                     /note="color: #00ff15"
     misc_feature    2347
                     /label=Type 2
                     /note="color: #a6acb3"
     promoter        2348..2373
                     /label=CYC1 promoter
                     /label=CYC1
                     /note="vntifkey=29"
                     /note="color: #ffffff"
     misc_feature    2374..2467
                     /label=5xQUAS fixed
                     /note="color: #a6acb3"
     promoter        2469..2650
                     /label=CYC1 promoter
                     /label=CYC1
                     /note="vntifkey=29"
                     /note="color: #ffffff"
     TATA_signal     2474..2482
                     /label=TATA-1
                     /note="vntifkey=41"
                     /note="color: #a6acb3"
     TATA_signal     2528..2536
                     /label=TATA-2
                     /note="vntifkey=41"
                     /note="color: #a6acb3"
     protein_bind    2651..2654
                     /label=BsaI
                     /note="color: #00ff2c"
     CDS             2655..3368
                     /label=mTurquoise2
                     /note="color: #40e0d0; direction: RIGHT"
     protein_bind    3371..3374
                     /label=BsaI
                     /note="color: #00ff2c"
     terminator      3384..3608
                     /label=ScADH1 Terminator
                     /note="color: #ff8eff"
     protein_bind    3609..3612
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    3613..3637
                     /label=ConE scar
                     /note="color: #fffd6c"
     protein_bind    3613..3616
                     /label=BsmBI
                     /note="color: #00ff15"
     protein_bind    3653..3656
                     /label=BsaI
                     /note="color: #00ff2c"
     promoter        3657..3833
                     /label=ScURA3 Promoter
                     /note="color: #ffb334"
     CDS             3834..4637
                     /label=URA3
                     /note="color: #ff9127; direction: RIGHT"
     terminator      4638..4715
                     /label=ScURA3 Terminator
                     /note="color: #ffcb72"
     protein_bind    4716..4719
                     /label=BsaI
                     /note="color: #00ff2c"
     misc_feature    4720..4739
                     /label=HO 3' barcode
                     /note="color: #0e7002"
     misc_feature    4740..5239
                     /label=HO 3' Homology
                     /note="color: #6685ca"
ORIGIN
        1 ccgagcggcc gcgtgttaca accaattaac caattctgat tagaaaaact catcgagcat
       61 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      121 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      181 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      241 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      301 aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      361 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga ggcgaaatac
      421 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      481 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
      541 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
      601 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
      661 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
      721 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
      781 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg
      841 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
      901 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
      961 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagtcatgac caaaatccct
     1021 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1081 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1141 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1201 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1261 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1321 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1381 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1441 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1501 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1561 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1621 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1681 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1741 ttatcccctg attctgtgga taaccgtgcg gccgccaatt ctaattgtat cgagatcact
     1801 tttcgtgatc cgctaatcag cgacggtcac attaggtttg ccaagtcagg gtatgaacca
     1861 tacgatcagt tttcgtgaac ctggtacgta tattgtggcg tttgtgtata ttttcattct
     1921 ttgacaacaa tcaataccaa cctcaaatag gaaaagtaat aagtttggcg ttacacccca
     1981 aaagacgcca aacggatcga acttactcaa tagcaattag cgagacaaaa cctacgttaa
     2041 gacctgtaac cgatttatca aagcactctg cggttctttc ttgggaatat tacctggaca
     2101 ttttgtgccc tcaagaaacg aggctctacg agcctgttgg agcccctcag acattagccg
     2161 ccacgaatca aactttttac gcgattcggc ccaaatcagt ttctcacaga tcattcgtag
     2221 agtgaaaaag cacatcgatt atttgatacc cctttgggtt aattactgtt gaggtctttg
     2281 ccgtaagttc catcgctgtc cctgaattcg catctagact gaactggccg ataattgcag
     2341 acgaacgcca gcttaagaat aaattaaact agtgggtaat cgcttatcct aggataaaca
     2401 attatcctca agggtaatcg cttatcctct agggtaatcg cttatcctag ggtaatcgct
     2461 tatcctttct agatatatat atgtgtgcga cgacacatga tcatatggca tgcatgtgct
     2521 ctgtatgtat ataaaactct tgttttcttc ttttctctaa atattctttc cttatacatt
     2581 aggacctttg cagcataaat tactatactt ctatagacac acaaacacaa atacacacac
     2641 taaattaata tatggtttct aaaggtgaag aattattcac tggtgttgtc ccaattttgg
     2701 ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg
     2761 atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa ttgccagttc
     2821 catggccaac cttagtcact actttatctt ggggtgttca atgttttgca agatacccag
     2881 atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa
     2941 gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag
     3001 gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca
     3061 ttttaggtca caaattggaa tacaattatt tctctgacaa tgtttacatc actgctgaca
     3121 aacaaaagaa tggtatcaaa gctaacttca aaattagaca caacattgaa gatggtggtg
     3181 ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac
     3241 cagacaacca ttacttatcc actcaatcta agttatccaa agatccaaac gaaaagaggg
     3301 accacatggt cttgttagaa tttgttactg ctgctggtat taccttgggt atggatgaat
     3361 tgtacaaagg atcctaactc gaggcgaatt tcttatgatt tatgattttt attattaaat
     3421 aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa
     3481 aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg
     3541 aggtcgctct tattgaccac acctctaccg gcatgccgag caaatgcctg caaatcgctc
     3601 cccatttcgc tgagcagtta cagagatgtt acgaaccact agtgcactgc agtacacggt
     3661 ttccttgaaa tttttttgat tcggtaatct ccgaacagaa ggaagaacga aggaaggagc
     3721 acagacttag attggtatat atacgcatat gtagtgttga agaaacatga aattgcccag
     3781 tattcttaac ccaactgcac agaacaaaaa cgtgcaggaa acgaagataa atcatgtcga
     3841 aagctacata taaggaacgt gctgctactc atcctagtcc tgttgctgcc aagctattta
     3901 atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg
     3961 aattactgga gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg
     4021 atatcttgac tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca
     4081 agtacaattt tttactcttc gaggacagaa aatttgctga cattggtaat acagtcaaat
     4141 tgcagtactc tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg
     4201 gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg
     4261 aacctagagg ccttttgatg ttagcagaat tgtcatgcaa gggctcccta tctactggag
     4321 aatatactaa gggtactgtt gacattgcga agagcgacaa agattttgtt atcggcttta
     4381 ttgctcaaag agacatgggt ggaagagatg aaggttacga ttggttgatt atgacacccg
     4441 gtgtgggttt agatgacaag ggagatgcat tgggtcaaca gtatagaacc gtggatgatg
     4501 tggtttctac aggatctgac attattattg ttggaagagg actatttgca aagggaaggg
     4561 atgctaaggt agagggtgaa cgttacagaa aagcaggctg ggaagcatat ttgagaagat
     4621 gcggccagca aaactaaaaa actgtattat aagtaaatgc atgtatacta aactcacaaa
     4681 ttagagcttc aatttaatta tatcagttat tacccgagtt atcgtgttgc atctgcggct
     4741 ttaaattgat gtatctcatc gcaggcacgg gcagtacagt gccctgagcg tagggaaaaa
     4801 tgaaaaaaag gatgtaactt ttaacataat tccagcacgc agcgattggg tataatgaag
     4861 attgttaagt tcaacaacat gtgaaattct tagctccgca acttgcttga attatgagct
     4921 ctaagattca agaagtaaat aggtcatata atggaatgat agtagtactt gataaaggaa
     4981 ccatgtgatc ttacgttgat atgaaaataa ttctctcaca gaagtcagtt tgtatgacta
     5041 acatagagag taattgcttt tcggataaaa tcgcacctaa caaagattac cttttgtaaa
     5101 gcctccagaa cagctatgaa tgttcgttat tctacgcact ttctttcata catcttgatc
     5161 cgcctttttt gatgtaggtt ttaacgcggc aaaacatatt aatttttctg atttggaaaa
     5221 ataaagagtt agttctgcc
//
