LOCUS       Exported                3336 bp ds-DNA     circular SYN 01-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    ptz697_mE_eGFP_Viral2A.str
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 1, 2025 from SnapGene Viewer 4.0.2
            http://www.snapgene.com
COMMENT     Insert from New DNA 120 to 941
            Backbone from H:/Documents/Plasmids/pTZ 180 pDONOR221.ape 2896to651
            
            2A sequence is the P2A sequence with the GSG at the N terminus.
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    536..553
                     /locus_tag="M13Fw"
                     /label=M13Fw
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=M13Fw
                     ApEinfo_revcolor=green"
     misc_recomb     570..651
                     /locus_tag="attP1"
                     /label=attP1
                     /note="ApEinfo_fwdcolor=plum
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=attP1
                     ApEinfo_revcolor=plum"
     misc_recomb     570..651
                     /locus_tag="attL1"
                     /label=attL1
                     /note="ApEinfo_fwdcolor=plum
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=attL1
                     ApEinfo_revcolor=plum"
     misc_recomb     645..651
                     /locus_tag="attB1"
                     /label=attB1
                     /note="ApEinfo_fwdcolor=#dda0dd
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=attB1
                     ApEinfo_revcolor=#dda0dd"
     misc_recomb     645..651
                     /locus_tag="attR1"
                     /label=attR1
                     /note="ApEinfo_fwdcolor=plum
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=attR1
                     ApEinfo_revcolor=plum"
     misc_feature    652..668
                     /locus_tag="attB1seq"
                     /label=attB1seq
                     /note="ApEinfo_fwdcolor=#cccccc
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=attB1seq
                     ApEinfo_revcolor=#cccccc"
     misc_feature    join(676,669..673)
                     /locus_tag="F primer"
                     /label=F primer
                     /note="ApEinfo_fwdcolor=#26b2ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=F primer
                     ApEinfo_revcolor=green"
     misc_feature    676
                     /locus_tag="Rabbit B-globin intron"
                     /label=Rabbit B-globin intron
                     /note="ApEinfo_fwdcolor=#ffa61b
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=Rabbit B-globin intron
                     ApEinfo_revcolor=#ff9c15"
     misc_feature    677..1393
                     /locus_tag="EGFP"
                     /label=EGFP
                     /note="ApEinfo_fwdcolor=#34ff49
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=EGFP
                     ApEinfo_revcolor=green"
     misc_feature    910..929
                     /locus_tag="4809_FWD"
                     /label=4809_FWD
                     /note="ApEinfo_fwdcolor=#808080
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=4809_FWD
                     ApEinfo_revcolor=green"
     misc_feature    1129..1150
                     /locus_tag="6492_REV"
                     /label=6492_REV
                     /note="ApEinfo_fwdcolor=#808080
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=6492_REV
                     ApEinfo_revcolor=green"
     misc_feature    1312..1330
                     /locus_tag="6488_FWD"
                     /label=6488_FWD
                     /note="ApEinfo_fwdcolor=#808080
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=6488_FWD
                     ApEinfo_revcolor=green"
     misc_feature    1374..1459
                     /locus_tag="GFPnostop_viral2A primer R"
                     /label=GFPnostop_viral2A primer R
                     /note="ApEinfo_fwdcolor=#66ccff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=GFPnostop_viral2A primer R
                     ApEinfo_revcolor=#66ccff"
     misc_feature    1383..1393
                     /locus_tag="GFP no stop"
                     /label=GFP no stop
                     /note="ApEinfo_fwdcolor=#00ff80
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=GFP no stop
                     ApEinfo_revcolor=#00ff80"
     misc_feature    complement(1384..1393)
                     /locus_tag="R Primer"
                     /label=R Primer
                     /note="ApEinfo_fwdcolor=#00ffff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=R Primer
                     ApEinfo_revcolor=#00ffff"
     misc_feature    1394..1459
                     /locus_tag="viral 2A"
                     /label=viral 2A
                     /note="ApEinfo_fwdcolor=#ffcc66
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=viral 2A
                     ApEinfo_revcolor=#ffcc66"
     misc_feature    1460..1470
                     /locus_tag="attB2 Reverse sequence"
                     /label=attB2 Reverse sequence
                     /note="ApEinfo_fwdcolor=#cccccc
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=attB2 Reverse sequence
                     ApEinfo_revcolor=#cccccc"
     misc_recomb     complement(1471..1559)
                     /locus_tag="attP2"
                     /label=attP2
                     /note="ApEinfo_fwdcolor=plum
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=attP2
                     ApEinfo_revcolor=plum"
     misc_recomb     complement(1471..1559)
                     /locus_tag="attL2"
                     /label=attL2
                     /note="ApEinfo_fwdcolor=plum
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=attL2
                     ApEinfo_revcolor=plum"
     misc_recomb     complement(1471..1484)
                     /locus_tag="attB2"
                     /label=attB2
                     /note="ApEinfo_fwdcolor=#dda0dd
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=attB2
                     ApEinfo_revcolor=#dda0dd"
     misc_recomb     complement(1471..1484)
                     /locus_tag="attR2"
                     /label=attR2
                     /note="ApEinfo_fwdcolor=plum
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=attR2
                     ApEinfo_revcolor=plum"
     misc_feature    complement(1471..1477)
                     /locus_tag="Gateway Recomb2"
                     /label=Gateway Recomb2
                     /note="ApEinfo_fwdcolor=#ff0000
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=Gateway Recomb2
                     ApEinfo_revcolor=#ff0000"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      721 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      781 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      841 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
      901 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
      961 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     1021 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     1081 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     1141 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     1201 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     1261 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     1321 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     1381 cgagctgtac aagggatccg gagccacgaa cttctctctg ttaaagcaag caggagacgt
     1441 ggaagaaaac cccggtcctg acccagcttt cttgtacaaa gttggcatta taagaaagca
     1501 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     1561 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     1621 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     1681 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     1741 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     1801 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     1861 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     1921 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     1981 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     2041 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     2101 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     2161 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     2221 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     2281 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     2341 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     2401 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     2461 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     2521 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     2581 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     2641 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2701 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2761 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2821 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2881 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2941 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3001 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3061 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3121 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3181 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3241 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3301 gttcctggcc ttttgctggc cttttgctca catgtt
//
