LOCUS       Exported                8993 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T15.
ACCESSION   T15
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4658)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=43.9"
                     /note="/other=misc_feature"
     CDS             complement(6340..7992)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(8039..8538)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(8595..8878)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactcaa atttaagacc ctctcaaact
     4681 aacaggctta gtgatgtaat tatggttagt agaggtacac ttgtgaataa agagggtggg
     4741 tgggtataga tgttgctaac agcaacacaa agcttttgca tattgcatac tattaaacat
     4801 gctgtacata ctttttgggt ttatttggaa aggaatgcaa agatgaaggt ctgttttgtg
     4861 tacttttaag actttggtta ttttactttt tggaaaagaa taaaccaaga attgattggg
     4921 cacatcattt caagaagtcc cctctcctcc acatttgttt tgccaatttg cacattaaat
     4981 gactcttccc tcaaatgtgt actatggggt aaaaggggta gggtttaaag atgtagacag
     5041 ttgggttttt taagggccct ttttcaataa ctggaacact ctataacaaa ggatacttat
     5101 ttaaatagat gacattgact atttttgttt ttattaaaag gaagcttaca tgcctaccaa
     5161 tatttaatct tttatgattg cctttttata actttttata ttctcagcag agtgctttac
     5221 caattgaagt aaaatgtggc aggctggagt tattgaagca gagtggcagt cttcagtttg
     5281 cagagtaggg gtctgtcttt taaactctga gtgcaaactt cagagttctt gccttggctg
     5341 cagttttttt ccttcaagaa tgcagtacta acatttattt gagtggagtt actgaacagt
     5401 aacatagctg tgatttttgg tatttgaaac actggtttta aatattttga cttgttgagg
     5461 gtatgtttta tatagcaaga cattatatag cagtaaaaaa tggtgtttta tcttctatat
     5521 aattcctgtt tttattatta acaaaacagt cctaaatagc agccctcaat tgtgaaaaaa
     5581 tttactttaa actacattag gttgtgaatg caggttttat cagaactatg tttttgttca
     5641 gtttatctgt tcatatggat aaatattggt tgggatgact tggtgtctaa tgtgtagtgc
     5701 tacacaccta acttatgggg ccaaaatagc atgtcctaat gcttgctgct gatttaaaca
     5761 cattaaaggt actttgcagg aaatccttgc accatgggat taatatccaa ttgctgcttg
     5821 tacactcatt cattactaaa agttttgaga aatttttttt tccagtaatg agcttaagaa
     5881 atttgtggaa aataactcac ctggcatctt acatctgaaa taaggaatga tataaggttt
     5941 ttttttctca cagaagatga agcacacagg aacctaatgg gccaactggg atgaggtgac
     6001 tattctgaga tgactattca gtggctaact tgggttagga agaaaataat taggtatttt
     6061 ctccaaatgt tcactggtac tctgccactt tatttctctc atctgttaca caaagaacca
     6121 ccaggaaagc aaatcagttt ggttggtaac tctgtaattc ctaactatca ctggtttggt
     6181 tctggactaa aactacattg acagattgaa tttgcctaat atgatgactg tttttaatat
     6241 ggatctgtat gtgttctatt cagcacaagg aaataaaatt ttagttgagg attcagcact
     6301 aaaaactcga ggctagcgag ctcgtttaaa caactagaat tacacggcga tcttgccgcc
     6361 cttcttggcc ttaatgagaa tctcgcggat cttgcgggcg tccaacttgc cggtcagtcc
     6421 tttaggcacc tcgtccacga acacaacacc accgcgcagc ttcttggcgg ttgtaacctg
     6481 gctggccaca tagtccacga tctccttctc ggtcatggtt ttaccgtgtt ccagcacgac
     6541 gactgcggcg ggcagctcgc cggcatcgtc gtcgggcagg ccggcgaccc cggcgtcgaa
     6601 gatgttgggg tgttgcagca ggatgctctc cagttcggct ggggctacct ggtagccctt
     6661 gtatttgatc aggctcttca gccggtccac gatgaagaag tgctcgtcct cgtcccagta
     6721 ggcgatgtcg ccgctgtgca gccagccgtc cttgtcgatg agagcgtttg tagcctcggg
     6781 gttgttaacg tagccgctca tgatcatggg gccacggacg cacagctcgc cgcgctggtt
     6841 cacacccagt gtcttaccgg tgtccaagtc caccacctta gcctcgaaga agggcaccac
     6901 cttgcctact gcgccaggct tgtcgtcccc ttcgggggtg atcagaatgg cgctggttgt
     6961 ttctgtcagg ccgtagccct ggcggatgcc tggtaggtgg aagcgtttgg ccacggcctc
     7021 acctacctcc ttgctgagcg gcgccccgcc gctggcgatc tcgtgcaagt tgcttaggtc
     7081 gtacttgtcg atgagagtgc tcttagcgaa gaagctaaat agtgtgggca ccagcagggc
     7141 agattgaatc ttatagtctt gcaagctgcg caagaatagc tcctcctcga agcggtacat
     7201 gagcacgacc cgaaagccgc agatcaagta gcccagcgtg gtgaacatgc cgaagccgtg
     7261 gtgaaatggc accacgctga ggatagcggt gtcggggatg atctggttgc cgaagatggg
     7321 gtcgcgggca tgactgaatc ggacacaagc ggtgcggtgc ggtagggcta cgcccttggg
     7381 caatccggta ctgccactac tgttcatgat cagggcgatg gttttgtccc ggtcgaagct
     7441 ctcgggcacg aagtcgtact cgttgaagcc gggtggcaaa tgggaagtca cgaaggtgta
     7501 catgctttgg aagccctggt agtcggtctt gctatccatg atgatgatct tttgtatgat
     7561 cggtagcttc ttttgcacgt tgaggatctt ttgcagccct ttcttgctca cgaatacgac
     7621 ggtgggctgg ctgatgccca tgctgttcag cagctcgcgc tcgttgtaga tgtcgttagc
     7681 tggggccaca gccacaccga tgaacagggc acccaacacg ggcatgaaga actgcaagct
     7741 attctcgctg cacaccacga tccgatggtt tgtattcagc ccatagcgct tcatagcttc
     7801 tgccagccga acgctcatct cgaagtactc ggcgtaggta atgtccacct cgatatgtgc
     7861 gtcggtaaag gcgatggtgc cgggcaccag ggcgtagcgc ttcatggctt tgtgcagctg
     7921 ctcgccggcg gtcccgtctt cgagtgggta gaatggcgct gggcccttct taatgttttt
     7981 ggcatcttcc atggtggctt taccaacagt accggattgc caagcttggg ctgcaggtcg
     8041 aaaggcccgg agatgaggaa gaggagaaca gcgcggcaga cgtgcgcttt tgaagcgtgc
     8101 agaatgccgg gcctccggag gaccttcggg cgcccgcccc gcccctgagc ccgcccctga
     8161 gcccgccccc ggacccaccc cttcccagcc tctgagccca gaaagcgaag gagcaaagct
     8221 gctattggcc gctgccccaa aggcctaccc gcttccattg ctcagcggtg ctgtccatct
     8281 gcacgagact agtgagacgt gctacttcca tttgtcacgt cctgcacgac gcgagctgcg
     8341 gggcgggggg gaacttcctg actaggggag gagtagaagg tggcgcgaag gggccaccaa
     8401 agaacggagc cggttggcgc ctaccggtgg atgtggaatg tgtgcgaggc cagaggccac
     8461 ttgtgtagcg ccaagtgccc agcggggctg ctaaagcgca tgctccagac tgccttggga
     8521 aaagcgcctc ccctacccgg tagatctcga tcctctacgc cggacgcatc gtggccggag
     8581 tactgcggtt tatctcgatg gctacgaggg cagacagtaa gtggatttac cataatccct
     8641 taattgtacg caccgctaaa acgcgttcag cgcgatcacg gcagcagaca ggtaaaaatg
     8701 gcaacaaacc acccgaaaaa ctgccgcaat cgcgcctgat aaattttaac cgtatgaata
     8761 cctatgcaac cagagggtac aggccacatt acccccactt aatccactga agctgccatt
     8821 tttcatggtt tcaccatccc agcgaagggc catccagcgt gcgttcctgt atttccggac
     8881 tataaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8941 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
