LOCUS       Exported                8260 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  31_1.
ACCESSION   31_1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             4696..4797
                     /label=C9orf85 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=73.5"
                     /note="/match_length=19.0"
                     /note="/other=CDS"
     CDS             complement(4876..5010)
                     /label=TINCR (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=64.4"
                     /note="/match_length=37.5"
                     /note="/other=CDS"
     CDS             complement(5607..7259)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7306..7805)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(7862..8145)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactatt ggtaggggtg gggaattttt
     4681 tttttttttt cgagatggag tcttgctctg ttgaccaggc tggagtgcag tggcacgatc
     4741 tcggctcact gcagcctccg ccttctgagt tcaagcaatt ctcctgcctc agcctccccg
     4801 ggtagctggg attacaggca tgtgccacta cgcccggcta acttttgtat ttttggcaga
     4861 gatggggttt cacaatgttg gccaggctgg tcgtgaactc ctgacctcaa gtaatcgacc
     4921 cgccttggcc tcccaaagtg ctgggattac aggggtgagc cactgagccc agccaggggt
     4981 gggggattta aaacagaaaa ctacactccc aggaggccaa cttcttccag ccaagaaaag
     5041 gacaggagaa aggtcagggt gagggatgag gagacgccta atgccagtat aggagcatat
     5101 ttgtgtgtgc acaactaaat gcatacacac aatacctgga cacccttttt ctggagtcca
     5161 ggctggtgcc ataagtgtgt gtgtgtcgct gatgggaagc ccagagggtg gggcccctac
     5221 ctataatagg aactcagacc caagaaggga cactgctgcg tggtgactct gcttcctgtc
     5281 tggggcagct ggaagggaca gtgacccaca actgccccag gagacagtgg agcagatcct
     5341 ctaaaagctg ggatggggag taggctggga gggctgagga aggggatgtc aaccaagagt
     5401 atttgcactt cccagaggtg agagggagca aagtggctgg ggcgtggcct ggcggggagc
     5461 tccctggctg actgggaaag acagcttgtc ctctactttg ccctcaacac atctaccaga
     5521 gccccagaca ctgaaatggg gctgggatcg tgaaagagga acggcagctc tactcgaggc
     5581 tagcgagctc gtttaaacaa ctagaattac acggcgatct tgccgccctt cttggcctta
     5641 atgagaatct cgcggatctt gcgggcgtcc aacttgccgg tcagtccttt aggcacctcg
     5701 tccacgaaca caacaccacc gcgcagcttc ttggcggttg taacctggct ggccacatag
     5761 tccacgatct ccttctcggt catggtttta ccgtgttcca gcacgacgac tgcggcgggc
     5821 agctcgccgg catcgtcgtc gggcaggccg gcgaccccgg cgtcgaagat gttggggtgt
     5881 tgcagcagga tgctctccag ttcggctggg gctacctggt agcccttgta tttgatcagg
     5941 ctcttcagcc ggtccacgat gaagaagtgc tcgtcctcgt cccagtaggc gatgtcgccg
     6001 ctgtgcagcc agccgtcctt gtcgatgaga gcgtttgtag cctcggggtt gttaacgtag
     6061 ccgctcatga tcatggggcc acggacgcac agctcgccgc gctggttcac acccagtgtc
     6121 ttaccggtgt ccaagtccac caccttagcc tcgaagaagg gcaccacctt gcctactgcg
     6181 ccaggcttgt cgtccccttc gggggtgatc agaatggcgc tggttgtttc tgtcaggccg
     6241 tagccctggc ggatgcctgg taggtggaag cgtttggcca cggcctcacc tacctccttg
     6301 ctgagcggcg ccccgccgct ggcgatctcg tgcaagttgc ttaggtcgta cttgtcgatg
     6361 agagtgctct tagcgaagaa gctaaatagt gtgggcacca gcagggcaga ttgaatctta
     6421 tagtcttgca agctgcgcaa gaatagctcc tcctcgaagc ggtacatgag cacgacccga
     6481 aagccgcaga tcaagtagcc cagcgtggtg aacatgccga agccgtggtg aaatggcacc
     6541 acgctgagga tagcggtgtc ggggatgatc tggttgccga agatggggtc gcgggcatga
     6601 ctgaatcgga cacaagcggt gcggtgcggt agggctacgc ccttgggcaa tccggtactg
     6661 ccactactgt tcatgatcag ggcgatggtt ttgtcccggt cgaagctctc gggcacgaag
     6721 tcgtactcgt tgaagccggg tggcaaatgg gaagtcacga aggtgtacat gctttggaag
     6781 ccctggtagt cggtcttgct atccatgatg atgatctttt gtatgatcgg tagcttcttt
     6841 tgcacgttga ggatcttttg cagccctttc ttgctcacga atacgacggt gggctggctg
     6901 atgcccatgc tgttcagcag ctcgcgctcg ttgtagatgt cgttagctgg ggccacagcc
     6961 acaccgatga acagggcacc caacacgggc atgaagaact gcaagctatt ctcgctgcac
     7021 accacgatcc gatggtttgt attcagccca tagcgcttca tagcttctgc cagccgaacg
     7081 ctcatctcga agtactcggc gtaggtaatg tccacctcga tatgtgcgtc ggtaaaggcg
     7141 atggtgccgg gcaccagggc gtagcgcttc atggctttgt gcagctgctc gccggcggtc
     7201 ccgtcttcga gtgggtagaa tggcgctggg cccttcttaa tgtttttggc atcttccatg
     7261 gtggctttac caacagtacc ggattgccaa gcttgggctg caggtcgaaa ggcccggaga
     7321 tgaggaagag gagaacagcg cggcagacgt gcgcttttga agcgtgcaga atgccgggcc
     7381 tccggaggac cttcgggcgc ccgccccgcc cctgagcccg cccctgagcc cgcccccgga
     7441 cccacccctt cccagcctct gagcccagaa agcgaaggag caaagctgct attggccgct
     7501 gccccaaagg cctacccgct tccattgctc agcggtgctg tccatctgca cgagactagt
     7561 gagacgtgct acttccattt gtcacgtcct gcacgacgcg agctgcgggg cgggggggaa
     7621 cttcctgact aggggaggag tagaaggtgg cgcgaagggg ccaccaaaga acggagccgg
     7681 ttggcgccta ccggtggatg tggaatgtgt gcgaggccag aggccacttg tgtagcgcca
     7741 agtgcccagc ggggctgcta aagcgcatgc tccagactgc cttgggaaaa gcgcctcccc
     7801 tacccggtag atctcgatcc tctacgccgg acgcatcgtg gccggagtac tgcggtttat
     7861 ctcgatggct acgagggcag acagtaagtg gatttaccat aatcccttaa ttgtacgcac
     7921 cgctaaaacg cgttcagcgc gatcacggca gcagacaggt aaaaatggca acaaaccacc
     7981 cgaaaaactg ccgcaatcgc gcctgataaa ttttaaccgt atgaatacct atgcaaccag
     8041 agggtacagg ccacattacc cccacttaat ccactgaagc tgccattttt catggtttca
     8101 ccatcccagc gaagggccat ccagcgtgcg ttcctgtatt tccggactat aaaaggatct
     8161 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8221 actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
