LOCUS       Exported                9519 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T28.
ACCESSION   T28
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             4672..4821
                     /label=C9orf85 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=74.0"
                     /note="/match_length=27.9"
                     /note="/other=CDS"
     CDS             complement(4857..4934)
                     /label=NANOGNB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=92.3"
                     /note="/match_length=13.8"
                     /note="/other=CDS"
     CDS             5818..5919
                     /label=CCNJL (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=73.5"
                     /note="/match_length=7.8"
                     /note="/other=CDS"
     CDS             5924..6094
                     /label=GVQW1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=78.9"
                     /note="/match_length=29.2"
                     /note="/other=CDS"
     CDS             complement(6866..8518)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(8565..9064)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(9121..9404)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactttt tttttttgag atggagtctt
     4681 gctctgtcgc ccaggctgga gtgcagtggc gcgatcttgg ctcactgcaa gctccgcctc
     4741 ccaggttcac accattctcc tgccgcagcc tcccaagtag ctgggactac aggcgcctgc
     4801 cactgcgccc ggctaatttt tttttcgtat ttttagtaga gacggggttt caccgcggtc
     4861 tcgatctcct gacctcgtga tccgctcgcc tcggcctccc aaagtgctgg gattacaggc
     4921 gtgagccacc ttgccggcct tatcagataa tttatttaaa agaaaagaga gagagagaga
     4981 taaatgggta gattttcaca atgtatacac caaaagagga catcttttcc gtgagtcagg
     5041 gaatatttaa agagatatca aaagtcccta ttttcatgag ttccacatca catattttgt
     5101 gctttctttg aatagccaaa aaattttctt ggggaaaata cgagagtaaa accagagcta
     5161 aggagctata agaatttgga atggcttctt gaaatcatgc cgctccaagt atgtcataac
     5221 ctcgatcact caaaaataca taactcccaa accaagaatt tctgagggca aaagcacacc
     5281 agcgtgtata ggaaaggcag gggaagggat ggaagggaga gaaagaggga acaatgtaga
     5341 tttcctgttt tataccagga aaactaggcc aaactcaatt taaatgaata gctccaaagc
     5401 agtgcagtca gaaaaagttc tcagtgtgac atagacttca gggaaacaac acgtcctgaa
     5461 agaaacatga ttcccctcaa gccacaaagg attttctcat caagtgtttt cacctctgca
     5521 ttagatttgg acacaagaag aggagagcat ttactcaggt aaaaatagtt ctcttagtct
     5581 cttcctctag ttactaattt ttaatttaaa aatacaatta actgatctag ctgataaaag
     5641 tcacagacag aaataagcta agttctctct tcctttaggg aacgctggtg gcaattcacc
     5701 atataaactg gatggaagaa ttctcccagg gacactggaa atagcatctt cttatcaaat
     5761 ggaaggaggt acgccctaaa atttgggtgg gacactcaat tttcttttat attttgagac
     5821 ggagtttcac tcttgttgcc ctgactggag tgcaatggcg cgatcttggc tcactgcaac
     5881 ctccacctcc tgggttcaag tgaatctcct gcctcagcct ctgagtagct gggattacag
     5941 gcctgcacca ccacgcccgg ctaattttgc atatttttta gtagaggcgg ggtttcacca
     6001 tgttggccag gctgatctcg aactcctgac ctcaggtgat ccacccgcct cagcctccca
     6061 aactactggc attacaggcg tgagccacca tgcccgctca aaactcaatt ttctaggctc
     6121 ttttatctga ggaatctctg gccagcaact cataacatct aaatcaatgt ttctatgcca
     6181 agcttctatt tcattaaaaa tatatatctc tctctagatt ctttttgtat ctcttcatgt
     6241 taagtgcatg gaaattcatc ctattacagg gtctgatcat gcaagcacac aaggcttcat
     6301 tcaaagggca acagcaagaa gaaagagaaa gcaagtttgc aagggtacaa aacacatagc
     6361 gcttaaaatt agactaatgc tggcccattc caaaagagct gcgaggcacc tggactgaaa
     6421 acaaaatgaa aaaactaaaa caaggacaag gaaataaaac ttggtcattg gtgaaactgg
     6481 acttccagcc ccctctggag aggagctgct aaaacaatcc ctctccagga agacctcaga
     6541 atagttagaa tagaataggt agcttcctct ccactgagta tgcctacagt tctaacctag
     6601 tgacttcccc aaattaaata cactgcgttg atattacaca cacacacaca cacacacaca
     6661 cacacacaca cagcccagct ggatgggccc ctccctccca aagagcatcc acaggaatat
     6721 gagtgacata aaggcctctg actaccctcc aacaaggtag agaaactgag tcacagcctc
     6781 cttctctcaa gtcctccctc cctccctccc tcccatggca ctgtgctcca actcgaggct
     6841 agcgagctcg tttaaacaac tagaattaca cggcgatctt gccgcccttc ttggccttaa
     6901 tgagaatctc gcggatcttg cgggcgtcca acttgccggt cagtccttta ggcacctcgt
     6961 ccacgaacac aacaccaccg cgcagcttct tggcggttgt aacctggctg gccacatagt
     7021 ccacgatctc cttctcggtc atggttttac cgtgttccag cacgacgact gcggcgggca
     7081 gctcgccggc atcgtcgtcg ggcaggccgg cgaccccggc gtcgaagatg ttggggtgtt
     7141 gcagcaggat gctctccagt tcggctgggg ctacctggta gcccttgtat ttgatcaggc
     7201 tcttcagccg gtccacgatg aagaagtgct cgtcctcgtc ccagtaggcg atgtcgccgc
     7261 tgtgcagcca gccgtccttg tcgatgagag cgtttgtagc ctcggggttg ttaacgtagc
     7321 cgctcatgat catggggcca cggacgcaca gctcgccgcg ctggttcaca cccagtgtct
     7381 taccggtgtc caagtccacc accttagcct cgaagaaggg caccaccttg cctactgcgc
     7441 caggcttgtc gtccccttcg ggggtgatca gaatggcgct ggttgtttct gtcaggccgt
     7501 agccctggcg gatgcctggt aggtggaagc gtttggccac ggcctcacct acctccttgc
     7561 tgagcggcgc cccgccgctg gcgatctcgt gcaagttgct taggtcgtac ttgtcgatga
     7621 gagtgctctt agcgaagaag ctaaatagtg tgggcaccag cagggcagat tgaatcttat
     7681 agtcttgcaa gctgcgcaag aatagctcct cctcgaagcg gtacatgagc acgacccgaa
     7741 agccgcagat caagtagccc agcgtggtga acatgccgaa gccgtggtga aatggcacca
     7801 cgctgaggat agcggtgtcg gggatgatct ggttgccgaa gatggggtcg cgggcatgac
     7861 tgaatcggac acaagcggtg cggtgcggta gggctacgcc cttgggcaat ccggtactgc
     7921 cactactgtt catgatcagg gcgatggttt tgtcccggtc gaagctctcg ggcacgaagt
     7981 cgtactcgtt gaagccgggt ggcaaatggg aagtcacgaa ggtgtacatg ctttggaagc
     8041 cctggtagtc ggtcttgcta tccatgatga tgatcttttg tatgatcggt agcttctttt
     8101 gcacgttgag gatcttttgc agccctttct tgctcacgaa tacgacggtg ggctggctga
     8161 tgcccatgct gttcagcagc tcgcgctcgt tgtagatgtc gttagctggg gccacagcca
     8221 caccgatgaa cagggcaccc aacacgggca tgaagaactg caagctattc tcgctgcaca
     8281 ccacgatccg atggtttgta ttcagcccat agcgcttcat agcttctgcc agccgaacgc
     8341 tcatctcgaa gtactcggcg taggtaatgt ccacctcgat atgtgcgtcg gtaaaggcga
     8401 tggtgccggg caccagggcg tagcgcttca tggctttgtg cagctgctcg ccggcggtcc
     8461 cgtcttcgag tgggtagaat ggcgctgggc ccttcttaat gtttttggca tcttccatgg
     8521 tggctttacc aacagtaccg gattgccaag cttgggctgc aggtcgaaag gcccggagat
     8581 gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct
     8641 ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc gcccccggac
     8701 ccaccccttc ccagcctctg agcccagaaa gcgaaggagc aaagctgcta ttggccgctg
     8761 ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac gagactagtg
     8821 agacgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc gggggggaac
     8881 ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa cggagccggt
     8941 tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa
     9001 gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag cgcctcccct
     9061 acccggtaga tctcgatcct ctacgccgga cgcatcgtgg ccggagtact gcggtttatc
     9121 tcgatggcta cgagggcaga cagtaagtgg atttaccata atcccttaat tgtacgcacc
     9181 gctaaaacgc gttcagcgcg atcacggcag cagacaggta aaaatggcaa caaaccaccc
     9241 gaaaaactgc cgcaatcgcg cctgataaat tttaaccgta tgaataccta tgcaaccaga
     9301 gggtacaggc cacattaccc ccacttaatc cactgaagct gccatttttc atggtttcac
     9361 catcccagcg aagggccatc cagcgtgcgt tcctgtattt ccggactata aaaggatcta
     9421 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     9481 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
