LOCUS       Exported                8347 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T30.
ACCESSION   T30
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8347
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             4616..4945
                     /label=GVQW1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=58.8"
                     /note="/match_length=56.4"
                     /note="/other=CDS"
     CDS             complement(5694..7346)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7393..7892)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(7949..8232)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactatt ctgtattttt ttttgagatg
     4681 gagtttcact cttgttgccc aggctggagt acagtggcgc aatcttggct gactgcaacc
     4741 tccgcctccc gggttcaagt gattctcctg cctcagcctc ccgagtagct gggattacag
     4801 gcacatgcca ccatgcctgg ctaattttgt atttttagta gagacaggat ttcaccatgt
     4861 tggccaggct ggtctcgaac tcctgacctc aggtgattca cccgcctcgg cctcccaaag
     4921 tgctgggatt tcaagcatga gccactgcac ccggccgtaa tgattcttgg agggtgccaa
     4981 gagtgacatc tccttggcag ctcatcctat ccacagggaa cccaggggtc ctggtttttg
     5041 tacaaggggc tctgctgctc tctgcacatg atggtctagt aagccccttg ccaaaccacc
     5101 ccagcctgga tgctcccaga ggaccacgac tgaagccttg actggagcca gcatttggga
     5161 gcccaggagc aggtcttggc ccaagagagg ccagggcaga ggaagcatgc tcacagatta
     5221 cagtgtagtg aaggaagttt atttacggat gtggagagaa catggtaaag gcccctgggc
     5281 tggccagcaa gggggcaatc agtggagatt gaaaacagat tctccaagcc agggccaggg
     5341 aaaagggctg gggaaccagc aggagggggg cggaggggca gagctgacct gaacctagag
     5401 aagagagaac atggccaaga attatccagg tagaagaaac agaggaatct aaagggggtg
     5461 tatgtccaac cccaagggtg ccccaaaggc acagtggcag tgaggcttgc tctggcctag
     5521 tcctccagca aattccatca ctgctgatgg actgggcacc atttcagccg ttctcagagg
     5581 tagcccgccc aggccaggca tgctggacca caggctcttg accttgactg ccctctgctt
     5641 ctggggctgg cggtctccac tcgaggctag cgagctcgtt taaacaacta gaattacacg
     5701 gcgatcttgc cgcccttctt ggccttaatg agaatctcgc ggatcttgcg ggcgtccaac
     5761 ttgccggtca gtcctttagg cacctcgtcc acgaacacaa caccaccgcg cagcttcttg
     5821 gcggttgtaa cctggctggc cacatagtcc acgatctcct tctcggtcat ggttttaccg
     5881 tgttccagca cgacgactgc ggcgggcagc tcgccggcat cgtcgtcggg caggccggcg
     5941 accccggcgt cgaagatgtt ggggtgttgc agcaggatgc tctccagttc ggctggggct
     6001 acctggtagc ccttgtattt gatcaggctc ttcagccggt ccacgatgaa gaagtgctcg
     6061 tcctcgtccc agtaggcgat gtcgccgctg tgcagccagc cgtccttgtc gatgagagcg
     6121 tttgtagcct cggggttgtt aacgtagccg ctcatgatca tggggccacg gacgcacagc
     6181 tcgccgcgct ggttcacacc cagtgtctta ccggtgtcca agtccaccac cttagcctcg
     6241 aagaagggca ccaccttgcc tactgcgcca ggcttgtcgt ccccttcggg ggtgatcaga
     6301 atggcgctgg ttgtttctgt caggccgtag ccctggcgga tgcctggtag gtggaagcgt
     6361 ttggccacgg cctcacctac ctccttgctg agcggcgccc cgccgctggc gatctcgtgc
     6421 aagttgctta ggtcgtactt gtcgatgaga gtgctcttag cgaagaagct aaatagtgtg
     6481 ggcaccagca gggcagattg aatcttatag tcttgcaagc tgcgcaagaa tagctcctcc
     6541 tcgaagcggt acatgagcac gacccgaaag ccgcagatca agtagcccag cgtggtgaac
     6601 atgccgaagc cgtggtgaaa tggcaccacg ctgaggatag cggtgtcggg gatgatctgg
     6661 ttgccgaaga tggggtcgcg ggcatgactg aatcggacac aagcggtgcg gtgcggtagg
     6721 gctacgccct tgggcaatcc ggtactgcca ctactgttca tgatcagggc gatggttttg
     6781 tcccggtcga agctctcggg cacgaagtcg tactcgttga agccgggtgg caaatgggaa
     6841 gtcacgaagg tgtacatgct ttggaagccc tggtagtcgg tcttgctatc catgatgatg
     6901 atcttttgta tgatcggtag cttcttttgc acgttgagga tcttttgcag ccctttcttg
     6961 ctcacgaata cgacggtggg ctggctgatg cccatgctgt tcagcagctc gcgctcgttg
     7021 tagatgtcgt tagctggggc cacagccaca ccgatgaaca gggcacccaa cacgggcatg
     7081 aagaactgca agctattctc gctgcacacc acgatccgat ggtttgtatt cagcccatag
     7141 cgcttcatag cttctgccag ccgaacgctc atctcgaagt actcggcgta ggtaatgtcc
     7201 acctcgatat gtgcgtcggt aaaggcgatg gtgccgggca ccagggcgta gcgcttcatg
     7261 gctttgtgca gctgctcgcc ggcggtcccg tcttcgagtg ggtagaatgg cgctgggccc
     7321 ttcttaatgt ttttggcatc ttccatggtg gctttaccaa cagtaccgga ttgccaagct
     7381 tgggctgcag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg cagacgtgcg
     7441 cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct
     7501 gagcccgccc ctgagcccgc ccccggaccc accccttccc agcctctgag cccagaaagc
     7561 gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc
     7621 ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc acgtcctgca
     7681 cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag aaggtggcgc
     7741 gaaggggcca ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg
     7801 aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag cgcatgctcc
     7861 agactgcctt gggaaaagcg cctcccctac ccggtagatc tcgatcctct acgccggacg
     7921 catcgtggcc ggagtactgc ggtttatctc gatggctacg agggcagaca gtaagtggat
     7981 ttaccataat cccttaattg tacgcaccgc taaaacgcgt tcagcgcgat cacggcagca
     8041 gacaggtaaa aatggcaaca aaccacccga aaaactgccg caatcgcgcc tgataaattt
     8101 taaccgtatg aatacctatg caaccagagg gtacaggcca cattaccccc acttaatcca
     8161 ctgaagctgc catttttcat ggtttcacca tcccagcgaa gggccatcca gcgtgcgttc
     8221 ctgtatttcc ggactataaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8281 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8341 gatcttc
//
