LOCUS       Exported                8458 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T31.
ACCESSION   T31
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8458
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             5487..5576
                     /label=NANOGNB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=90.0"
                     /note="/match_length=16.0"
                     /note="/other=CDS"
     CDS             complement(5609..5764)
                     /label=C9orf85 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=71.2"
                     /note="/match_length=29.1"
                     /note="/other=CDS"
     CDS             complement(5805..7457)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7504..8003)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(8060..8343)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactgct gctgtggagc gggcatggcc
     4681 gggccctgct gaggaggggc ctgggcagag ggcctggacc tgggatcaag gcccacgcgc
     4741 ttccctgggg tttactgagg tgatgggtgc aggaaaggtt cacaaatgtg gagtgtctgc
     4801 gtccaataca cgcgtgtgct cctctcctta ctccatcgtg tgtgccttgg gtctcagctg
     4861 ctgacacgca gcctgctctg gagcctgcag atgagatccg ggagactgac acgaagccag
     4921 cagaggtcag aggggactct gaccacagcc cgctctctgg ctgtctgtct gcagtgcccg
     4981 gctgagggtg ggaggcaaac acgccttgtt cctgctcttc ccagttcagc ttggtgggag
     5041 aaagtcattc gcgtggctcg ggacgctcat gtaaatttgg ttttggtgct caagggttct
     5101 ttcctcccag gggcaggtgt ttctttcctg tttgtcttgt gtcttgagag cttggcctta
     5161 tgaccagtga gaactctctc cctggtctct gccagcccaa gcatcactgc ccgaggcgcc
     5221 agctcagttt caccgtccac gtccacaagg ggcttttccc accttcacct ttgtcgctgg
     5281 gtcagtgctg gaaagcgccc ctcactcctg cgctgacaag ggcccttctc tactgtctgt
     5341 ggggtggttc cgggctgggg gggctgcctc ctttgcacct gattttgaag gtgtctcttt
     5401 catccatggt taagtcataa aaagcttatt ggttttggtt ttgactcacc tgaaagtttt
     5461 tttggtttaa aagaagaata ggcggggcac ggtggctcat gcctgtaatc ccagcacttt
     5521 gggaggctga ggcaggtgga tcacgaggtc aggagatcga caccatcctg gctaacacgg
     5581 tgaaaccccg tctctactaa aaaatacaaa aaattagctg ggtgtggtgg tgggggtggg
     5641 cgcctgtagt cccagctacg tgggaggctg aggcagcaga ctggtgtgaa cccgggaggt
     5701 ggagcttgca gtgagccgag atcgcgccac tgcactccag cctgggcgac agagcgagac
     5761 tccatctcaa ctcgaggcta gcgagctcgt ttaaacaact agaattacac ggcgatcttg
     5821 ccgcccttct tggccttaat gagaatctcg cggatcttgc gggcgtccaa cttgccggtc
     5881 agtcctttag gcacctcgtc cacgaacaca acaccaccgc gcagcttctt ggcggttgta
     5941 acctggctgg ccacatagtc cacgatctcc ttctcggtca tggttttacc gtgttccagc
     6001 acgacgactg cggcgggcag ctcgccggca tcgtcgtcgg gcaggccggc gaccccggcg
     6061 tcgaagatgt tggggtgttg cagcaggatg ctctccagtt cggctggggc tacctggtag
     6121 cccttgtatt tgatcaggct cttcagccgg tccacgatga agaagtgctc gtcctcgtcc
     6181 cagtaggcga tgtcgccgct gtgcagccag ccgtccttgt cgatgagagc gtttgtagcc
     6241 tcggggttgt taacgtagcc gctcatgatc atggggccac ggacgcacag ctcgccgcgc
     6301 tggttcacac ccagtgtctt accggtgtcc aagtccacca ccttagcctc gaagaagggc
     6361 accaccttgc ctactgcgcc aggcttgtcg tccccttcgg gggtgatcag aatggcgctg
     6421 gttgtttctg tcaggccgta gccctggcgg atgcctggta ggtggaagcg tttggccacg
     6481 gcctcaccta cctccttgct gagcggcgcc ccgccgctgg cgatctcgtg caagttgctt
     6541 aggtcgtact tgtcgatgag agtgctctta gcgaagaagc taaatagtgt gggcaccagc
     6601 agggcagatt gaatcttata gtcttgcaag ctgcgcaaga atagctcctc ctcgaagcgg
     6661 tacatgagca cgacccgaaa gccgcagatc aagtagccca gcgtggtgaa catgccgaag
     6721 ccgtggtgaa atggcaccac gctgaggata gcggtgtcgg ggatgatctg gttgccgaag
     6781 atggggtcgc gggcatgact gaatcggaca caagcggtgc ggtgcggtag ggctacgccc
     6841 ttgggcaatc cggtactgcc actactgttc atgatcaggg cgatggtttt gtcccggtcg
     6901 aagctctcgg gcacgaagtc gtactcgttg aagccgggtg gcaaatggga agtcacgaag
     6961 gtgtacatgc tttggaagcc ctggtagtcg gtcttgctat ccatgatgat gatcttttgt
     7021 atgatcggta gcttcttttg cacgttgagg atcttttgca gccctttctt gctcacgaat
     7081 acgacggtgg gctggctgat gcccatgctg ttcagcagct cgcgctcgtt gtagatgtcg
     7141 ttagctgggg ccacagccac accgatgaac agggcaccca acacgggcat gaagaactgc
     7201 aagctattct cgctgcacac cacgatccga tggtttgtat tcagcccata gcgcttcata
     7261 gcttctgcca gccgaacgct catctcgaag tactcggcgt aggtaatgtc cacctcgata
     7321 tgtgcgtcgg taaaggcgat ggtgccgggc accagggcgt agcgcttcat ggctttgtgc
     7381 agctgctcgc cggcggtccc gtcttcgagt gggtagaatg gcgctgggcc cttcttaatg
     7441 tttttggcat cttccatggt ggctttacca acagtaccgg attgccaagc ttgggctgca
     7501 ggtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag
     7561 cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc
     7621 cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca
     7681 aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc
     7741 catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag
     7801 ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc
     7861 accaaagaac ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag
     7921 gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct
     7981 tgggaaaagc gcctccccta cccggtagat ctcgatcctc tacgccggac gcatcgtggc
     8041 cggagtactg cggtttatct cgatggctac gagggcagac agtaagtgga tttaccataa
     8101 tcccttaatt gtacgcaccg ctaaaacgcg ttcagcgcga tcacggcagc agacaggtaa
     8161 aaatggcaac aaaccacccg aaaaactgcc gcaatcgcgc ctgataaatt ttaaccgtat
     8221 gaatacctat gcaaccagag ggtacaggcc acattacccc cacttaatcc actgaagctg
     8281 ccatttttca tggtttcacc atcccagcga agggccatcc agcgtgcgtt cctgtatttc
     8341 cggactataa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8401 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
