LOCUS       Exported                8874 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  26_1.
ACCESSION   26_1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8874
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             5487..5576
                     /label=NANOGNB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=90.0"
                     /note="/match_length=16.0"
                     /note="/other=CDS"
     CDS             complement(5609..5764)
                     /label=C9orf85 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=71.2"
                     /note="/match_length=29.1"
                     /note="/other=CDS"
     CDS             complement(6221..7873)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7920..8419)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(8476..8759)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactgct gctgtggagc gggcatggcc
     4681 gggccctgct gaggaggggc ctgggcagag ggcctggacc tgggatcaag gcccacgcgc
     4741 ttccctgggg tttactgagg tgatgggtgc aggaaaggtt cacaaatgtg gagtgtctgc
     4801 gtccaataca cgcgtgtgct cctctcctta ctccatcgtg tgtgccttgg gtctcagctg
     4861 ctgacacgca gcctgctctg gagcctgcag atgagatccg ggagactgac acgaagccag
     4921 cagaggtcag aggggactct gaccacagcc cgctctctgg ctgtctgtct gcagtgcccg
     4981 gctgagggtg ggaggcaaac acgccttgtt cctgctcttc ccagttcagc ttggtgggag
     5041 aaagtcattc gcgtggctcg ggacgctcat gtaaatttgg ttttggtgct caagggttct
     5101 ttcctcccag gggcaggtgt ttctttcctg tttgtcttgt gtcttgagag cttggcctta
     5161 tgaccagtga gaactctctc cctggtctct gccagcccaa gcatcactgc ccgaggcgcc
     5221 agctcagttt caccgtccac gtccacaagg ggcttttccc accttcacct ttgtcgctgg
     5281 gtcagtgctg gaaagcgccc ctcactcctg cgctgacaag ggcccttctc tactgtctgt
     5341 ggggtggttc cgggctgggg gggctgcctc ctttgcacct gattttgaag gtgtctcttt
     5401 catccatggt taagtcataa aaagcttatt ggttttggtt ttgactcacc tgaaagtttt
     5461 tttggtttaa aagaagaata ggcggggcac ggtggctcat gcctgtaatc ccagcacttt
     5521 gggaggctga ggcaggtgga tcacgaggtc aggagatcga caccatcctg gctaacacgg
     5581 tgaaaccccg tctctactaa aaaatacaaa aaattagctg ggtgtggtgg tgggggtggg
     5641 cgcctgtagt cccagctacg tgggaggctg aggcagcaga ctggtgtgaa cccgggaggt
     5701 ggagcttgca gtgagccgag atcgcgccac tgcactccag cctgggcgac agagcgagac
     5761 tccatctcaa aaaaaaaaaa agaaagaaag aataataatt ttgggagttt ctggaaaggt
     5821 actaggattt ctcaaaagga tttgtcttct gccttgtgaa agacagatgt cagactaatc
     5881 aggcttatcc gatgtgctac atgagatgga aagcgtgtga aatagtaagt cacactaagt
     5941 cttctggagg ttctatttac gggtttggtt ttgatatgaa tgttgcagaa actctataaa
     6001 attcctagaa atctgatacg ttatcctatg atatatatcc taactcacat gctattccta
     6061 gaaatctggt gcaatgttat cagtcataat tttggttatt acattaaaat gctgcatgcc
     6121 acagaaataa ccaagtttcc ttgccaaccg tgtcacgaat ataataaact ctcatcagat
     6181 cctgaactcg aggctagcga gctcgtttaa acaactagaa ttacacggcg atcttgccgc
     6241 ccttcttggc cttaatgaga atctcgcgga tcttgcgggc gtccaacttg ccggtcagtc
     6301 ctttaggcac ctcgtccacg aacacaacac caccgcgcag cttcttggcg gttgtaacct
     6361 ggctggccac atagtccacg atctccttct cggtcatggt tttaccgtgt tccagcacga
     6421 cgactgcggc gggcagctcg ccggcatcgt cgtcgggcag gccggcgacc ccggcgtcga
     6481 agatgttggg gtgttgcagc aggatgctct ccagttcggc tggggctacc tggtagccct
     6541 tgtatttgat caggctcttc agccggtcca cgatgaagaa gtgctcgtcc tcgtcccagt
     6601 aggcgatgtc gccgctgtgc agccagccgt ccttgtcgat gagagcgttt gtagcctcgg
     6661 ggttgttaac gtagccgctc atgatcatgg ggccacggac gcacagctcg ccgcgctggt
     6721 tcacacccag tgtcttaccg gtgtccaagt ccaccacctt agcctcgaag aagggcacca
     6781 ccttgcctac tgcgccaggc ttgtcgtccc cttcgggggt gatcagaatg gcgctggttg
     6841 tttctgtcag gccgtagccc tggcggatgc ctggtaggtg gaagcgtttg gccacggcct
     6901 cacctacctc cttgctgagc ggcgccccgc cgctggcgat ctcgtgcaag ttgcttaggt
     6961 cgtacttgtc gatgagagtg ctcttagcga agaagctaaa tagtgtgggc accagcaggg
     7021 cagattgaat cttatagtct tgcaagctgc gcaagaatag ctcctcctcg aagcggtaca
     7081 tgagcacgac ccgaaagccg cagatcaagt agcccagcgt ggtgaacatg ccgaagccgt
     7141 ggtgaaatgg caccacgctg aggatagcgg tgtcggggat gatctggttg ccgaagatgg
     7201 ggtcgcgggc atgactgaat cggacacaag cggtgcggtg cggtagggct acgcccttgg
     7261 gcaatccggt actgccacta ctgttcatga tcagggcgat ggttttgtcc cggtcgaagc
     7321 tctcgggcac gaagtcgtac tcgttgaagc cgggtggcaa atgggaagtc acgaaggtgt
     7381 acatgctttg gaagccctgg tagtcggtct tgctatccat gatgatgatc ttttgtatga
     7441 tcggtagctt cttttgcacg ttgaggatct tttgcagccc tttcttgctc acgaatacga
     7501 cggtgggctg gctgatgccc atgctgttca gcagctcgcg ctcgttgtag atgtcgttag
     7561 ctggggccac agccacaccg atgaacaggg cacccaacac gggcatgaag aactgcaagc
     7621 tattctcgct gcacaccacg atccgatggt ttgtattcag cccatagcgc ttcatagctt
     7681 ctgccagccg aacgctcatc tcgaagtact cggcgtaggt aatgtccacc tcgatatgtg
     7741 cgtcggtaaa ggcgatggtg ccgggcacca gggcgtagcg cttcatggct ttgtgcagct
     7801 gctcgccggc ggtcccgtct tcgagtgggt agaatggcgc tgggcccttc ttaatgtttt
     7861 tggcatcttc catggtggct ttaccaacag taccggattg ccaagcttgg gctgcaggtc
     7921 gaaaggcccg gagatgagga agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg
     7981 cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg
     8041 agcccgcccc cggacccacc ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc
     8101 tgctattggc cgctgcccca aaggcctacc cgcttccatt gctcagcggt gctgtccatc
     8161 tgcacgagac tagtgagacg tgctacttcc atttgtcacg tcctgcacga cgcgagctgc
     8221 ggggcggggg ggaacttcct gactagggga ggagtagaag gtggcgcgaa ggggccacca
     8281 aagaacggag ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca
     8341 cttgtgtagc gccaagtgcc cagcggggct gctaaagcgc atgctccaga ctgccttggg
     8401 aaaagcgcct cccctacccg gtagatctcg atcctctacg ccggacgcat cgtggccgga
     8461 gtactgcggt ttatctcgat ggctacgagg gcagacagta agtggattta ccataatccc
     8521 ttaattgtac gcaccgctaa aacgcgttca gcgcgatcac ggcagcagac aggtaaaaat
     8581 ggcaacaaac cacccgaaaa actgccgcaa tcgcgcctga taaattttaa ccgtatgaat
     8641 acctatgcaa ccagagggta caggccacat tacccccact taatccactg aagctgccat
     8701 ttttcatggt ttcaccatcc cagcgaaggg ccatccagcg tgcgttcctg tatttccgga
     8761 ctataaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8821 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
