LOCUS       Exported                8573 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  6_1.
ACCESSION   6_1
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             complement(5920..7572)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7619..8118)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(8175..8458)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgacttga gctcaaagta tgattttttt
     4681 tttggcaccg ggaggtagct tgaacaaatg caggggtgtg ggttggagtg gcgtgtctct
     4741 actaaaggct cctgggaagg agctcagagc ctggttgggg gtgctgcact ccagcgcaga
     4801 agccagggct tctgttggga gggagacagc tagtcacctg ggccctaaca tggaggtagg
     4861 agccaaggtg cctgtgcacc cctaacccca acctccaaat gagcagaatt gtggagagct
     4921 caaccactca acaggcttgt ggagaggctc acaccaggaa gaaatgctac acagcagcac
     4981 tgcagcctcc ccacactcgt ggctcccgtg gcccgctcac tcctcccctc tggtggtcaa
     5041 acatggcact gctctccaaa gtcaggggcc aggccagttt cgagagagac gatgggaaaa
     5101 cctgctcaac agggtggctg aagcctgaac cagctgaaca ggacggtccc atcacccccc
     5161 agcaacctag tccctctcct ggcccacaga acagagccag aaactccaca aaccactttt
     5221 attacccagt gggcgggctg ggctgtgatg ttggagaacc ttgggggtgg gggctgcgga
     5281 atgcagctga gcttctcctg gctctgtctg ctggtctagg ccagggtggg gctgatcaag
     5341 ggcagagagc tcaatcttgg gggaagagga agagaggata gagaggccaa acaggcctct
     5401 tcccctcctc ttcacccatg ccacagcatt aaataaacaa aaagcaactc tttacagcac
     5461 aaactacaca gggaagtcct tcctcccagc cctgggcgca cagcatggaa gaggagctcg
     5521 ggaggacagg gttggggcct ccagggtctg acaccacccc actcctcttc ctagcaccat
     5581 ggggtaggga caggcagctt tgaacagagg aaacaaaact ccaacttcct ccaaactcct
     5641 atgcaggcag gtccatctag tctggtactt ctggcccaag ggttttctgc tccttccaaa
     5701 cctttgagct ttctcaggac gtgagcttag ctgagacttc tccaagaggg gctggagccc
     5761 tggtctctga ggcacagtgg gcctgggaaa caggcaagag tctggttcca aagtttttcc
     5821 cagcccaggc ctctgccttg gcaagcagga agcagggcca ggtgagagcc ggtgagaccc
     5881 aggcactcga ggctagcgag ctcgtttaaa caactagaat tacacggcga tcttgccgcc
     5941 cttcttggcc ttaatgagaa tctcgcggat cttgcgggcg tccaacttgc cggtcagtcc
     6001 tttaggcacc tcgtccacga acacaacacc accgcgcagc ttcttggcgg ttgtaacctg
     6061 gctggccaca tagtccacga tctccttctc ggtcatggtt ttaccgtgtt ccagcacgac
     6121 gactgcggcg ggcagctcgc cggcatcgtc gtcgggcagg ccggcgaccc cggcgtcgaa
     6181 gatgttgggg tgttgcagca ggatgctctc cagttcggct ggggctacct ggtagccctt
     6241 gtatttgatc aggctcttca gccggtccac gatgaagaag tgctcgtcct cgtcccagta
     6301 ggcgatgtcg ccgctgtgca gccagccgtc cttgtcgatg agagcgtttg tagcctcggg
     6361 gttgttaacg tagccgctca tgatcatggg gccacggacg cacagctcgc cgcgctggtt
     6421 cacacccagt gtcttaccgg tgtccaagtc caccacctta gcctcgaaga agggcaccac
     6481 cttgcctact gcgccaggct tgtcgtcccc ttcgggggtg atcagaatgg cgctggttgt
     6541 ttctgtcagg ccgtagccct ggcggatgcc tggtaggtgg aagcgtttgg ccacggcctc
     6601 acctacctcc ttgctgagcg gcgccccgcc gctggcgatc tcgtgcaagt tgcttaggtc
     6661 gtacttgtcg atgagagtgc tcttagcgaa gaagctaaat agtgtgggca ccagcagggc
     6721 agattgaatc ttatagtctt gcaagctgcg caagaatagc tcctcctcga agcggtacat
     6781 gagcacgacc cgaaagccgc agatcaagta gcccagcgtg gtgaacatgc cgaagccgtg
     6841 gtgaaatggc accacgctga ggatagcggt gtcggggatg atctggttgc cgaagatggg
     6901 gtcgcgggca tgactgaatc ggacacaagc ggtgcggtgc ggtagggcta cgcccttggg
     6961 caatccggta ctgccactac tgttcatgat cagggcgatg gttttgtccc ggtcgaagct
     7021 ctcgggcacg aagtcgtact cgttgaagcc gggtggcaaa tgggaagtca cgaaggtgta
     7081 catgctttgg aagccctggt agtcggtctt gctatccatg atgatgatct tttgtatgat
     7141 cggtagcttc ttttgcacgt tgaggatctt ttgcagccct ttcttgctca cgaatacgac
     7201 ggtgggctgg ctgatgccca tgctgttcag cagctcgcgc tcgttgtaga tgtcgttagc
     7261 tggggccaca gccacaccga tgaacagggc acccaacacg ggcatgaaga actgcaagct
     7321 attctcgctg cacaccacga tccgatggtt tgtattcagc ccatagcgct tcatagcttc
     7381 tgccagccga acgctcatct cgaagtactc ggcgtaggta atgtccacct cgatatgtgc
     7441 gtcggtaaag gcgatggtgc cgggcaccag ggcgtagcgc ttcatggctt tgtgcagctg
     7501 ctcgccggcg gtcccgtctt cgagtgggta gaatggcgct gggcccttct taatgttttt
     7561 ggcatcttcc atggtggctt taccaacagt accggattgc caagcttggg ctgcaggtcg
     7621 aaaggcccgg agatgaggaa gaggagaaca gcgcggcaga cgtgcgcttt tgaagcgtgc
     7681 agaatgccgg gcctccggag gaccttcggg cgcccgcccc gcccctgagc ccgcccctga
     7741 gcccgccccc ggacccaccc cttcccagcc tctgagccca gaaagcgaag gagcaaagct
     7801 gctattggcc gctgccccaa aggcctaccc gcttccattg ctcagcggtg ctgtccatct
     7861 gcacgagact agtgagacgt gctacttcca tttgtcacgt cctgcacgac gcgagctgcg
     7921 gggcgggggg gaacttcctg actaggggag gagtagaagg tggcgcgaag gggccaccaa
     7981 agaacggagc cggttggcgc ctaccggtgg atgtggaatg tgtgcgaggc cagaggccac
     8041 ttgtgtagcg ccaagtgccc agcggggctg ctaaagcgca tgctccagac tgccttggga
     8101 aaagcgcctc ccctacccgg tagatctcga tcctctacgc cggacgcatc gtggccggag
     8161 tactgcggtt tatctcgatg gctacgaggg cagacagtaa gtggatttac cataatccct
     8221 taattgtacg caccgctaaa acgcgttcag cgcgatcacg gcagcagaca ggtaaaaatg
     8281 gcaacaaacc acccgaaaaa ctgccgcaat cgcgcctgat aaattttaac cgtatgaata
     8341 cctatgcaac cagagggtac aggccacatt acccccactt aatccactga agctgccatt
     8401 tttcatggtt tcaccatccc agcgaagggc catccagcgt gcgttcctgt atttccggac
     8461 tataaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
