LOCUS       Exported                8018 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T38.
ACCESSION   T38
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             4687..4854
                     /label=C9orf85 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=75.0"
                     /note="/match_length=31.3"
                     /note="/other=CDS"
     CDS             4850..4957
                     /label=YP007_HUMAN (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=71.1"
                     /note="/match_length=17.8"
                     /note="/other=CDS"
     CDS             complement(5365..7017)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7064..7563)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(7620..7903)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactagg aaaaaaattt tttttttgag
     4681 atgggtcttg ctctgttgcc caggctggag tacaacggcg caatcttggc tcactgcaac
     4741 ctctgcctcc caggttcaag caaatctcct gcttcagcct cctgagtagc tgggattact
     4801 ggtgtgtgcc accacacccg gctaattttt gtatttttag tagagatggg gttttcacca
     4861 tgttggtcag gctggtctcg aactcctgac ctcgtgatct gcctgcctca gcctcccaaa
     4921 gtgttgggat tacaggcgtg agccaccatg cctggccaac tcaggaaaaa tttttatggg
     4981 gtcacatctt ggggatggaa gagggacagg aaaagaaacc taagacaaaa gaactgatgg
     5041 tcttcccaac ccagaggctt cctgagttat cacccctgag agaggaagaa cacaatctct
     5101 ctccttaagg cacccagata agagtacagg caaactcttc acttgcccag tgcctgggtg
     5161 agggagcagg cttaagaggc agaagaggcc cagccctgta ggcactgggt actccctgct
     5221 accaaccctg aacccccggc agttgtccct gaggtctggg gagtagcaga acccagtgta
     5281 gaagccagca gacccagtga tgcccttgga gaccctctgc tgccttccca ctcgaggcta
     5341 gcgagctcgt ttaaacaact agaattacac ggcgatcttg ccgcccttct tggccttaat
     5401 gagaatctcg cggatcttgc gggcgtccaa cttgccggtc agtcctttag gcacctcgtc
     5461 cacgaacaca acaccaccgc gcagcttctt ggcggttgta acctggctgg ccacatagtc
     5521 cacgatctcc ttctcggtca tggttttacc gtgttccagc acgacgactg cggcgggcag
     5581 ctcgccggca tcgtcgtcgg gcaggccggc gaccccggcg tcgaagatgt tggggtgttg
     5641 cagcaggatg ctctccagtt cggctggggc tacctggtag cccttgtatt tgatcaggct
     5701 cttcagccgg tccacgatga agaagtgctc gtcctcgtcc cagtaggcga tgtcgccgct
     5761 gtgcagccag ccgtccttgt cgatgagagc gtttgtagcc tcggggttgt taacgtagcc
     5821 gctcatgatc atggggccac ggacgcacag ctcgccgcgc tggttcacac ccagtgtctt
     5881 accggtgtcc aagtccacca ccttagcctc gaagaagggc accaccttgc ctactgcgcc
     5941 aggcttgtcg tccccttcgg gggtgatcag aatggcgctg gttgtttctg tcaggccgta
     6001 gccctggcgg atgcctggta ggtggaagcg tttggccacg gcctcaccta cctccttgct
     6061 gagcggcgcc ccgccgctgg cgatctcgtg caagttgctt aggtcgtact tgtcgatgag
     6121 agtgctctta gcgaagaagc taaatagtgt gggcaccagc agggcagatt gaatcttata
     6181 gtcttgcaag ctgcgcaaga atagctcctc ctcgaagcgg tacatgagca cgacccgaaa
     6241 gccgcagatc aagtagccca gcgtggtgaa catgccgaag ccgtggtgaa atggcaccac
     6301 gctgaggata gcggtgtcgg ggatgatctg gttgccgaag atggggtcgc gggcatgact
     6361 gaatcggaca caagcggtgc ggtgcggtag ggctacgccc ttgggcaatc cggtactgcc
     6421 actactgttc atgatcaggg cgatggtttt gtcccggtcg aagctctcgg gcacgaagtc
     6481 gtactcgttg aagccgggtg gcaaatggga agtcacgaag gtgtacatgc tttggaagcc
     6541 ctggtagtcg gtcttgctat ccatgatgat gatcttttgt atgatcggta gcttcttttg
     6601 cacgttgagg atcttttgca gccctttctt gctcacgaat acgacggtgg gctggctgat
     6661 gcccatgctg ttcagcagct cgcgctcgtt gtagatgtcg ttagctgggg ccacagccac
     6721 accgatgaac agggcaccca acacgggcat gaagaactgc aagctattct cgctgcacac
     6781 cacgatccga tggtttgtat tcagcccata gcgcttcata gcttctgcca gccgaacgct
     6841 catctcgaag tactcggcgt aggtaatgtc cacctcgata tgtgcgtcgg taaaggcgat
     6901 ggtgccgggc accagggcgt agcgcttcat ggctttgtgc agctgctcgc cggcggtccc
     6961 gtcttcgagt gggtagaatg gcgctgggcc cttcttaatg tttttggcat cttccatggt
     7021 ggctttacca acagtaccgg attgccaagc ttgggctgca ggtcgaaagg cccggagatg
     7081 aggaagagga gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat gccgggcctc
     7141 cggaggacct tcgggcgccc gccccgcccc tgagcccgcc cctgagcccg cccccggacc
     7201 caccccttcc cagcctctga gcccagaaag cgaaggagca aagctgctat tggccgctgc
     7261 cccaaaggcc tacccgcttc cattgctcag cggtgctgtc catctgcacg agactagtga
     7321 gacgtgctac ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg ggggggaact
     7381 tcctgactag gggaggagta gaaggtggcg cgaaggggcc accaaagaac ggagccggtt
     7441 ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg tagcgccaag
     7501 tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc gcctccccta
     7561 cccggtagat ctcgatcctc tacgccggac gcatcgtggc cggagtactg cggtttatct
     7621 cgatggctac gagggcagac agtaagtgga tttaccataa tcccttaatt gtacgcaccg
     7681 ctaaaacgcg ttcagcgcga tcacggcagc agacaggtaa aaatggcaac aaaccacccg
     7741 aaaaactgcc gcaatcgcgc ctgataaatt ttaaccgtat gaatacctat gcaaccagag
     7801 ggtacaggcc acattacccc cacttaatcc actgaagctg ccatttttca tggtttcacc
     7861 atcccagcga agggccatcc agcgtgcgtt cctgtatttc cggactataa aaggatctag
     7921 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7981 tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
