LOCUS       Exported                8172 bp ds-DNA     circular SYN 04-OCT-2024
DEFINITION  T3.
ACCESSION   T3
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..8172
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4658)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=43.9"
                     /note="/other=misc_feature"
     CDS             complement(5519..7171)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(7218..7717)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(7774..8057)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactcgt tgttatattt taattggaat
     4681 cagacattta atggcataaa aacattgcat atatggcttt gctgttccga atgtcatgaa
     4741 cttaaaatcc aaatatgaca taagcagttt taagacttat tttggccagc ctccccaatc
     4801 ccaaaggaga tttaaaagta ataatgtaaa aaagttaagg tcaaggtgtt ttaaaatcaa
     4861 catctcagct aatgactaac cctttgtttc ctggggactt ctgctctact gtgaaaactg
     4921 ctgagcttaa cccgctgttg aacaactggt tagttattag ttccctgggt gattgtccct
     4981 gaagttaagc catgctctgg ggacacatga ggcactttaa ttggggtagt tactttttcc
     5041 aggttgggta caatgtttgg ctctcaaaag caaggtaaac agtttacagt caaaaggaat
     5101 gctctgtgat tgtgtgcctt tgggatcaag ggttgagatc ttagagtatt ctcataaaca
     5161 tctgtaccag gtgctcaaag atgtttgagg gttgtcaagc aagctgttca acttctgcat
     5221 tttctgtgga tatgggcaca atgacccact cagatttttc tcctagtgtc ctgaatgaca
     5281 gctcatctac acagagtgaa actccagata tgtgacaagc agcatgggac agatgctgac
     5341 aatcagaaca gatgctggat gtaaacaact ggtaaggctg cactggtgca tgctggcaga
     5401 atcacctgct atgcatccag ggagcaaatg catctgcatg ctcagcatga caccagctta
     5461 cctccctgtg caaatcccct cccactcgag gctagcgagc tcgtttaaac aactagaatt
     5521 acacggcgat cttgccgccc ttcttggcct taatgagaat ctcgcggatc ttgcgggcgt
     5581 ccaacttgcc ggtcagtcct ttaggcacct cgtccacgaa cacaacacca ccgcgcagct
     5641 tcttggcggt tgtaacctgg ctggccacat agtccacgat ctccttctcg gtcatggttt
     5701 taccgtgttc cagcacgacg actgcggcgg gcagctcgcc ggcatcgtcg tcgggcaggc
     5761 cggcgacccc ggcgtcgaag atgttggggt gttgcagcag gatgctctcc agttcggctg
     5821 gggctacctg gtagcccttg tatttgatca ggctcttcag ccggtccacg atgaagaagt
     5881 gctcgtcctc gtcccagtag gcgatgtcgc cgctgtgcag ccagccgtcc ttgtcgatga
     5941 gagcgtttgt agcctcgggg ttgttaacgt agccgctcat gatcatgggg ccacggacgc
     6001 acagctcgcc gcgctggttc acacccagtg tcttaccggt gtccaagtcc accaccttag
     6061 cctcgaagaa gggcaccacc ttgcctactg cgccaggctt gtcgtcccct tcgggggtga
     6121 tcagaatggc gctggttgtt tctgtcaggc cgtagccctg gcggatgcct ggtaggtgga
     6181 agcgtttggc cacggcctca cctacctcct tgctgagcgg cgccccgccg ctggcgatct
     6241 cgtgcaagtt gcttaggtcg tacttgtcga tgagagtgct cttagcgaag aagctaaata
     6301 gtgtgggcac cagcagggca gattgaatct tatagtcttg caagctgcgc aagaatagct
     6361 cctcctcgaa gcggtacatg agcacgaccc gaaagccgca gatcaagtag cccagcgtgg
     6421 tgaacatgcc gaagccgtgg tgaaatggca ccacgctgag gatagcggtg tcggggatga
     6481 tctggttgcc gaagatgggg tcgcgggcat gactgaatcg gacacaagcg gtgcggtgcg
     6541 gtagggctac gcccttgggc aatccggtac tgccactact gttcatgatc agggcgatgg
     6601 ttttgtcccg gtcgaagctc tcgggcacga agtcgtactc gttgaagccg ggtggcaaat
     6661 gggaagtcac gaaggtgtac atgctttgga agccctggta gtcggtcttg ctatccatga
     6721 tgatgatctt ttgtatgatc ggtagcttct tttgcacgtt gaggatcttt tgcagccctt
     6781 tcttgctcac gaatacgacg gtgggctggc tgatgcccat gctgttcagc agctcgcgct
     6841 cgttgtagat gtcgttagct ggggccacag ccacaccgat gaacagggca cccaacacgg
     6901 gcatgaagaa ctgcaagcta ttctcgctgc acaccacgat ccgatggttt gtattcagcc
     6961 catagcgctt catagcttct gccagccgaa cgctcatctc gaagtactcg gcgtaggtaa
     7021 tgtccacctc gatatgtgcg tcggtaaagg cgatggtgcc gggcaccagg gcgtagcgct
     7081 tcatggcttt gtgcagctgc tcgccggcgg tcccgtcttc gagtgggtag aatggcgctg
     7141 ggcccttctt aatgtttttg gcatcttcca tggtggcttt accaacagta ccggattgcc
     7201 aagcttgggc tgcaggtcga aaggcccgga gatgaggaag aggagaacag cgcggcagac
     7261 gtgcgctttt gaagcgtgca gaatgccggg cctccggagg accttcgggc gcccgccccg
     7321 cccctgagcc cgcccctgag cccgcccccg gacccacccc ttcccagcct ctgagcccag
     7381 aaagcgaagg agcaaagctg ctattggccg ctgccccaaa ggcctacccg cttccattgc
     7441 tcagcggtgc tgtccatctg cacgagacta gtgagacgtg ctacttccat ttgtcacgtc
     7501 ctgcacgacg cgagctgcgg ggcggggggg aacttcctga ctaggggagg agtagaaggt
     7561 ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc taccggtgga tgtggaatgt
     7621 gtgcgaggcc agaggccact tgtgtagcgc caagtgccca gcggggctgc taaagcgcat
     7681 gctccagact gccttgggaa aagcgcctcc cctacccggt agatctcgat cctctacgcc
     7741 ggacgcatcg tggccggagt actgcggttt atctcgatgg ctacgagggc agacagtaag
     7801 tggatttacc ataatccctt aattgtacgc accgctaaaa cgcgttcagc gcgatcacgg
     7861 cagcagacag gtaaaaatgg caacaaacca cccgaaaaac tgccgcaatc gcgcctgata
     7921 aattttaacc gtatgaatac ctatgcaacc agagggtaca ggccacatta cccccactta
     7981 atccactgaa gctgccattt ttcatggttt caccatccca gcgaagggcc atccagcgtg
     8041 cgttcctgta tttccggact ataaaaggat ctaggtgaag atcctttttg ataatctcat
     8101 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8161 caaaggatct tc
//
