LOCUS       Exported                9854 bp ds-DNA     circular SYN 10-JAN-2025
DEFINITION  Z6.
ACCESSION   Z6
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Mar 12, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..9854
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(747..1607)
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(1608..1712)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             1755..1847
                     /label=tnpA (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.8"
                     /note="/match_length=6.5"
                     /note="/other=CDS"
     polyA_signal    complement(1868..1916)
                     /label=poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1980..2772)
                     /label=NeoR/KanR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     CDS             complement(2823..3755)
                     /label=hRluc
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.7"
                     /note="/other=CDS"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4228..4279
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=36.9"
                     /note="/other=misc_feature"
     rep_origin      complement(4280..4312)
                     /direction=LEFT
                     /label=M13 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.0"
                     /note="/match_length=7.3"
                     /note="/other=rep_origin"
     CDS             4317..4385
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.1"
                     /note="/match_length=3.2"
                     /note="/other=CDS"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     terminator      complement(4551..4570)
                     /label=T7 terminator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=41.7"
                     /note="/other=terminator"
     CDS             complement(4578..4627)
                     /label=SET1 (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=39.7"
                     /note="/other=CDS"
     misc_feature    complement(4634..4657)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.1"
                     /note="/other=misc_feature"
     CDS             5962..6237
                     /label=C9orf85 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=37.0"
                     /note="/match_length=51.4"
                     /note="/other=CDS"
     CDS             complement(7201..8853)
                     /label=luciferase
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     promoter        complement(8900..9399)
                     /label=PGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    complement(9456..9739)
                     /label=cer region
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgacttgg ggtttttatt cctttattct
     4681 ttttccaaca tggaatgcaa atgtaatgac tgaagccaca gcagccatta tggactacaa
     4741 agtgatcttg agaaggaaac caaaatgatt gaagcagcat gggtcattca tatgcgggtc
     4801 attgatacgt gggtcattga tatgtgggtc attgatacac gggtcagtga tacattctct
     4861 accaccctgg atcactaact ccagacttct tttacataag aggtaaataa gcctctattt
     4921 gtataaaaca ctcgtcccat acaactgaac ataatctcaa ctgatacact taggaaatca
     4981 ttgaaggaat aagcattctg aaatattaag tatttggtgt actaaataca aaaggagaca
     5041 tacctctaat gcaattaaca atgccaagac tttatctttt taagggctct gagtgaccct
     5101 gtatatagag gtcacttgcg atggcttgtc attacagtac caaacaaacc cataggctca
     5161 ttaagcattt gtaagggtgc cgtccttaaa ggtgctggaa ggctcagaaa tcatgtttta
     5221 tactaaataa ttgccaaagt caaataatga cattatacat acttcccagt tttagagttt
     5281 tcagttgtgg ggatgtaaaa atgtttgtta aaggaattgt tttcaactag taaaacatca
     5341 tttttgagtg cctagtgtgc caagcactgt gctaaacact tacatcatct catttaacct
     5401 ttacaagcac atacaattat tcttctcact ttacagatgg gtaaagggat acataacttg
     5461 tccaaggtca catggcaaag ctggaaatca aatgaagttc agtcagattt caattctgtg
     5521 ttacaaaaca ccaaactaaa gcatcttcca tgtttatttt tttctagata gttttgagca
     5581 tccttaatgc ctttaaattc tccattgagt tacgttagaa tggttgtcct tgtttcagtg
     5641 aattagaagg ctgaaatcca gagagggctt gttcatcttt gaggtaagat caggacattt
     5701 agttatggca tcaagtttga cagagagaaa gccaaagcta tggctctgca aaccagctcg
     5761 gtcccttcct tgagtataat tttgtagtct agctcttttt tttttttttt tttcatagag
     5821 atggggtctc gctaaattgc ccaggttggt ctcgaattcc tggccctggc aatcctctca
     5881 cctcagcctt ccaaagtgct cagattacag acatgagcca ctgtgcctag cttagctctt
     5941 tttttttttt tttttttgag atggagtctt gcactgtcac ccaggctgga gtgcagtggc
     6001 acgatctcag ctcactgcaa cctccacctc ccaggttcaa gtgattctcc ttgcctcagc
     6061 ctcccaagta gctgggatta caggcgcccg ccaccgtgcc cggataattt tttgtatttt
     6121 tagtagagac agggtttcac tatgttggcc aggctgatct tgaactcctg accttgtgat
     6181 ccacccgcct cggcctccca aagtgctggg attacaggca tgagccacca cgcccggcca
     6241 tctagattag ttctttacat tgaaagtaac atccagggtc actgatattc tgagaaaatt
     6301 agaaccacct ttgagttaag acttctagaa agacacatac gttgttatat tttaattgga
     6361 atcagacatt taatggcata aaaacattgc atatatggct ttgctgttcc gaatgtcatg
     6421 aacttaaaat ccaaatatga cataagcagt tttaagactt attttggcca gcctccccaa
     6481 tcccaaagga gatttaaaag taataatgta aaaaagttaa ggtcaaggtg ttttaaaatc
     6541 aacatctcag ctaatgacta accctttgtt tcctggggac ttctgctcta ctgtgaaaac
     6601 tgctgagctt aacctgctgt tgaacaactg gttagttatt agttccctgg gtgattgtcc
     6661 ctgaagttaa gccatgctct ggggacacat gaggcacttt aattggggta gttacttttt
     6721 ccaggttggg tacaatgttt ggctctcaaa agcaaggtaa acagtttaca gtcaaaagga
     6781 atgctctgtg attgtgtgcc tttgggatca agggttgaga tcttagagta ttctcataaa
     6841 catctgtacc aggtgctcaa agatgtttga gggttgtcaa gcaagctgtt caacttctgc
     6901 attttctgtg gatatgggca caatgaccca ctcagatttt tctcctagtg tcctgaatga
     6961 cagctcatct acacagagtg aaactccaga tatgtgacaa gcagcatggg acagatgctg
     7021 acaatcagaa cagatgctgg atgtaaacaa ctggtaaggc tgcactggtg catgctggca
     7081 gaatcacctg ctatgcatcc agggagcaaa tgcgtctgca tgctcagcat gacaccagct
     7141 tacctccctg tgcaaatccc ctcccactcg aggctagcga gctcgtttaa acaactagaa
     7201 ttacacggcg atcttgccgc ccttcttggc cttaatgaga atctcgcgga tcttgcgggc
     7261 gtccaacttg ccggtcagtc ctttaggcac ctcgtccacg aacacaacac caccgcgcag
     7321 cttcttggcg gttgtaacct ggctggccac atagtccacg atctccttct cggtcatggt
     7381 tttaccgtgt tccagcacga cgactgcggc gggcagctcg ccggcatcgt cgtcgggcag
     7441 gccggcgacc ccggcgtcga agatgttggg gtgttgcagc aggatgctct ccagttcggc
     7501 tggggctacc tggtagccct tgtatttgat caggctcttc agccggtcca cgatgaagaa
     7561 gtgctcgtcc tcgtcccagt aggcgatgtc gccgctgtgc agccagccgt ccttgtcgat
     7621 gagagcgttt gtagcctcgg ggttgttaac gtagccgctc atgatcatgg ggccacggac
     7681 gcacagctcg ccgcgctggt tcacacccag tgtcttaccg gtgtccaagt ccaccacctt
     7741 agcctcgaag aagggcacca ccttgcctac tgcgccaggc ttgtcgtccc cttcgggggt
     7801 gatcagaatg gcgctggttg tttctgtcag gccgtagccc tggcggatgc ctggtaggtg
     7861 gaagcgtttg gccacggcct cacctacctc cttgctgagc ggcgccccgc cgctggcgat
     7921 ctcgtgcaag ttgcttaggt cgtacttgtc gatgagagtg ctcttagcga agaagctaaa
     7981 tagtgtgggc accagcaggg cagattgaat cttatagtct tgcaagctgc gcaagaatag
     8041 ctcctcctcg aagcggtaca tgagcacgac ccgaaagccg cagatcaagt agcccagcgt
     8101 ggtgaacatg ccgaagccgt ggtgaaatgg caccacgctg aggatagcgg tgtcggggat
     8161 gatctggttg ccgaagatgg ggtcgcgggc atgactgaat cggacacaag cggtgcggtg
     8221 cggtagggct acgcccttgg gcaatccggt actgccacta ctgttcatga tcagggcgat
     8281 ggttttgtcc cggtcgaagc tctcgggcac gaagtcgtac tcgttgaagc cgggtggcaa
     8341 atgggaagtc acgaaggtgt acatgctttg gaagccctgg tagtcggtct tgctatccat
     8401 gatgatgatc ttttgtatga tcggtagctt cttttgcacg ttgaggatct tttgcagccc
     8461 tttcttgctc acgaatacga cggtgggctg gctgatgccc atgctgttca gcagctcgcg
     8521 ctcgttgtag atgtcgttag ctggggccac agccacaccg atgaacaggg cacccaacac
     8581 gggcatgaag aactgcaagc tattctcgct gcacaccacg atccgatggt ttgtattcag
     8641 cccatagcgc ttcatagctt ctgccagccg aacgctcatc tcgaagtact cggcgtaggt
     8701 aatgtccacc tcgatatgtg cgtcggtaaa ggcgatggtg ccgggcacca gggcgtagcg
     8761 cttcatggct ttgtgcagct gctcgccggc ggtcccgtct tcgagtgggt agaatggcgc
     8821 tgggcccttc ttaatgtttt tggcatcttc catggtggct ttaccaacag taccggattg
     8881 ccaagcttgg gctgcaggtc gaaaggcccg gagatgagga agaggagaac agcgcggcag
     8941 acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc
     9001 cgcccctgag cccgcccctg agcccgcccc cggacccacc ccttcccagc ctctgagccc
     9061 agaaagcgaa ggagcaaagc tgctattggc cgctgcccca aaggcctacc cgcttccatt
     9121 gctcagcggt gctgtccatc tgcacgagac tagtgagacg tgctacttcc atttgtcacg
     9181 tcctgcacga cgcgagctgc ggggcggggg ggaacttcct gactagggga ggagtagaag
     9241 gtggcgcgaa ggggccacca aagaacggag ccggttggcg cctaccggtg gatgtggaat
     9301 gtgtgcgagg ccagaggcca cttgtgtagc gccaagtgcc cagcggggct gctaaagcgc
     9361 atgctccaga ctgccttggg aaaagcgcct cccctacccg gtagatctcg atcctctacg
     9421 ccggacgcat cgtggccgga gtactgcggt ttatctcgat ggctacgagg gcagacagta
     9481 agtggattta ccataatccc ttaattgtac gcaccgctaa aacgcgttca gcgcgatcac
     9541 ggcagcagac aggtaaaaat ggcaacaaac cacccgaaaa actgccgcaa tcgcgcctga
     9601 taaattttaa ccgtatgaat acctatgcaa ccagagggta caggccacat tacccccact
     9661 taatccactg aagctgccat ttttcatggt ttcaccatcc cagcgaaggg ccatccagcg
     9721 tgcgttcctg tatttccgga ctataaaagg atctaggtga agatcctttt tgataatctc
     9781 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9841 atcaaaggat cttc
//
